FastQCFastQC Report
Thu 26 May 2016
SRR937983_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937983_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3271995
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174960.5347196435202376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138180.42231115878844555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89270.2728304902666416No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA63840.19511032260134872No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA63100.19284870545340074No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA56300.17206627760739243No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA55780.1704770331250506No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC48270.14752467531276792No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA46610.14245131792683058No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG45360.13863101869043198No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA41990.1283314919491014No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA39620.12108820459688965No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34320.10489013583455965No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG34110.10424832556284469No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT33800.10330089135221784No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA32910.10058083829590203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73050.057.6701281
GTATCAA137100.043.7081681
TATCAAC174900.034.157442
ATCAACG176400.033.8939253
TCAACGC179950.033.2252774
CAACGCA180400.033.116055
AACGCAG185900.032.1810236
GTGGTAT32200.030.0781141
TGGTATC31350.028.9558072
ACGCAGA209900.028.2455087
CGCAGAG210900.028.0440038
GCAGAGT225700.025.5919139
AGGTAAG20100.025.3004072
GTGTAAG12800.024.973261
GTACATG112500.024.6396241
TACATGG111500.024.2536562
TAAGGTG23000.023.9701065
GAGTACT156000.023.53760512-13
ACATGGG113300.023.1552243
CAGAGTA215900.022.38665210-11
Produced by FastQC (version 0.11.5)