FastQCFastQC Report
Thu 26 May 2016
SRR937983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937983_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3271995
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170960.5224946859637621No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141190.4315104393496934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91670.2801654648005269No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA63220.19321545418009503No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA62530.191106649001603No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA58230.17796481962839186No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA57540.17585601444989982No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC54200.1656481748902428No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG46570.14232906835126583No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA45190.1381114579942818No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA42290.12924836376583704No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA39590.1209965174152161No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA36110.11036080434108242No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG33910.10363707768502092No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT33800.10330089135221784No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33750.10314807938276188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71950.056.4491
GTATCAA128350.041.90791
TATCAAC163850.032.6539152
ATCAACG165400.032.1144373
TCAACGC168800.031.5224824
CAACGCA170050.031.2338835
AACGCAG175550.030.3018116
TAAGGTG23550.027.8636255
TGGTATC30050.027.221572
ACGCAGA198600.026.6414187
CGCAGAG199400.026.4868978
AGGTAAG21500.026.3231412
GTGGTAT33150.025.9671481
GTAAGGT24650.025.0777854
AAGGTAA22250.024.367111
GCAGAGT218450.024.0683869
GTACATG111550.023.1504481
TACATGG109850.023.1190722
GAGTACT151200.022.60114312-13
GGTAAGG26750.022.2212723
Produced by FastQC (version 0.11.5)