##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937983_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3271995 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50142680535881 33.0 31.0 34.0 31.0 34.0 2 32.722137411579176 34.0 31.0 34.0 31.0 34.0 3 32.78486183505782 34.0 31.0 34.0 31.0 34.0 4 36.24314004147317 37.0 35.0 37.0 35.0 37.0 5 36.16801187043379 37.0 37.0 37.0 35.0 37.0 6 36.2247283385213 37.0 37.0 37.0 35.0 37.0 7 36.19741075398954 37.0 37.0 37.0 35.0 37.0 8 36.1890064012934 37.0 37.0 37.0 35.0 37.0 9 37.888462237870165 39.0 38.0 39.0 35.0 39.0 10-11 37.86031350903653 39.0 38.0 39.0 35.0 39.0 12-13 37.783000279645904 39.0 38.0 39.0 35.0 39.0 14-15 39.03218449294697 40.0 38.0 41.0 36.0 41.0 16-17 38.942949332135285 40.0 38.0 41.0 36.0 41.0 18-19 39.19967588581278 40.0 39.0 41.0 36.0 41.0 20-21 39.170536935417076 40.0 39.0 41.0 36.0 41.0 22-23 39.06967324216571 40.0 39.0 41.0 35.5 41.0 24-25 38.95444430691367 40.0 38.0 41.0 35.0 41.0 26-27 38.7893480888571 40.0 38.0 41.0 35.0 41.0 28-29 38.661840711859284 40.0 38.0 41.0 34.5 41.0 30-31 38.39757288748913 40.0 38.0 41.0 34.0 41.0 32-33 38.14955860262623 40.0 38.0 41.0 34.0 41.0 34-35 38.030821715803356 40.0 38.0 41.0 33.0 41.0 36-37 37.95487065230846 40.0 37.5 41.0 33.0 41.0 38-39 37.74827574614264 40.0 37.0 41.0 33.0 41.0 40-41 37.51109613553811 40.0 37.0 41.0 33.0 41.0 42-43 37.23009463645269 40.0 36.5 41.0 32.0 41.0 44-45 36.94637170900322 40.0 36.0 41.0 31.0 41.0 46-47 36.54893757478236 39.0 35.5 41.0 30.5 41.0 48-49 36.09222110669485 39.0 35.0 41.0 29.5 41.0 50-51 35.68031185866727 39.0 35.0 40.0 28.0 41.0 52-53 35.12816278753482 38.0 34.5 40.0 27.0 41.0 54-55 34.73277954275602 38.0 34.0 40.0 26.0 41.0 56-57 34.33616295257175 38.0 34.0 40.0 25.0 41.0 58-59 33.81368140843736 37.0 33.0 40.0 23.5 41.0 60-61 33.32126317430192 36.5 33.0 39.5 22.0 41.0 62-63 32.75726460462195 36.0 32.0 39.0 20.0 40.0 64-65 32.18251296227531 35.0 31.0 39.0 18.0 40.0 66-67 31.606175590121623 35.0 31.0 38.0 13.0 40.0 68-69 30.97475515702194 35.0 30.0 37.5 8.0 40.0 70-71 30.342512289902643 34.0 29.0 37.0 6.5 39.0 72-73 29.677272734218725 34.0 28.5 36.0 2.0 39.0 74-75 29.038837162037225 34.0 28.0 36.0 2.0 38.5 76-77 27.736629334702528 31.5 26.0 34.5 2.0 36.5 78-79 28.3928011809309 33.5 27.0 35.0 2.0 37.0 80-81 28.13034295590305 33.5 27.0 35.0 2.0 37.0 82-83 27.57802472192042 33.0 26.0 35.0 2.0 36.0 84-85 26.92652112854696 33.0 25.0 35.0 2.0 36.0 86-87 26.231846931306436 32.0 23.5 35.0 2.0 35.0 88-89 25.420971456252225 32.0 20.5 34.0 2.0 35.0 90-91 24.76681565833689 31.0 18.5 34.0 2.0 35.0 92-93 23.840501742820514 31.0 8.5 34.0 2.0 35.0 94-95 22.943809969147264 30.5 2.0 34.0 2.0 35.0 96-97 21.903658012924836 30.0 2.0 34.0 2.0 35.0 98-99 20.475044430080118 29.0 2.0 34.0 2.0 35.0 100-101 16.578983158592848 14.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21563645478525473 1101 2 0.18872502454322415 1101 3 0.18247389739467934 1101 4 0.06402666944386226 1101 5 -0.04162395179962175 1101 6 -0.04337905794627517 1101 7 0.0441398821157577 1101 8 0.11285001179027176 1101 9 0.08987852540645491 1101 10-11 0.16147027624639776 1101 12-13 0.25137326065159726 1101 14-15 0.40891221324609717 1101 16-17 0.40310241793167023 1101 18-19 0.35586668536664945 1101 20-21 0.2392955806493191 1101 22-23 0.31032535875266376 1101 24-25 0.3561464277399793 1101 26-27 0.400110919778065 1101 28-29 0.3543699394574773 1101 30-31 0.4608153634664802 1101 32-33 0.5117339356195245 1101 34-35 0.4148109374782294 1101 36-37 0.3598809996149015 1101 38-39 0.4851054539973916 1101 40-41 0.6420662097340681 1101 42-43 0.6155045961339312 1101 44-45 0.702122948820417 1101 46-47 0.6533150069579179 1101 48-49 0.7292032037260157 1101 50-51 0.5573972047245235 1101 52-53 0.5375601707139523 1101 54-55 0.8064832241555422 1101 56-57 0.9025779934819766 1101 58-59 0.9763225449657114 1101 60-61 0.981311760975764 1101 62-63 1.1125256775952082 1101 64-65 1.2911220922112463 1101 66-67 1.3261604509371523 1101 68-69 1.640735669979474 1101 70-71 1.6975974462073395 1101 72-73 1.7339649519854383 1101 74-75 1.8169235857001418 1101 76-77 1.8284396682269133 1101 78-79 1.9337529742741815 1101 80-81 2.002122608496329 1101 82-83 2.1089800943836075 1101 84-85 2.287362875532228 1101 86-87 2.405780142033116 1101 88-89 2.845911324107888 1101 90-91 3.179306141851999 1101 92-93 3.3418321658356156 1101 94-95 3.495660759746375 1101 96-97 3.692645569163961 1101 98-99 4.183827145353035 1101 100-101 4.0098875379218235 1102 1 0.16705718439452255 1102 2 0.18148441138763616 1102 3 0.17927742247916711 1102 4 0.07325280990844618 1102 5 -0.05798854838602807 1102 6 -0.02389987210849398 1102 7 0.07874817790704469 1102 8 0.14663398300493213 1102 9 0.14878805908493575 1102 10-11 0.20239250353645843 1102 12-13 0.2354851079334921 1102 14-15 0.284206548198469 1102 16-17 0.3129356325707988 1102 18-19 0.2556008903688749 1102 20-21 0.22163061571077947 1102 22-23 0.31765439242180094 1102 24-25 0.31035253689063325 1102 26-27 0.25276146803859234 1102 28-29 0.25973378386937895 1102 30-31 0.34732823445255434 1102 32-33 0.3835483965744615 1102 34-35 0.441063094645358 1102 36-37 0.48654085136716674 1102 38-39 0.4894615312739887 1102 40-41 0.6016440984709206 1102 42-43 0.6932492328896629 1102 44-45 0.7484328260452671 1102 46-47 0.705103088459488 1102 48-49 0.690090996360567 1102 50-51 0.9388909779465777 1102 52-53 0.9941252938225134 1102 54-55 1.0485153756146772 1102 56-57 1.1748956378421411 1102 58-59 1.2768188601108221 1102 60-61 1.2486134416323367 1102 62-63 1.2994531114265229 1102 64-65 1.387056549005223 1102 66-67 1.3946606096406384 1102 68-69 1.6714121202492613 1102 70-71 1.7132648810522255 1102 72-73 1.9898057070406132 1102 74-75 1.9817720972214836 1102 76-77 2.002001801308456 1102 78-79 1.94413391598599 1102 80-81 2.208244895533589 1102 82-83 2.2023320853788526 1102 84-85 2.5449154277243373 1102 86-87 2.677866666106702 1102 88-89 2.7874280893307564 1102 90-91 3.0294512421819455 1102 92-93 3.2292599157402613 1102 94-95 3.4660451555928766 1102 96-97 3.7282105535736783 1102 98-99 4.138859846272982 1102 100-101 3.8196250636944704 1103 1 0.16745446643736983 1103 2 0.17016872285675788 1103 3 0.13267212903843273 1103 4 0.08109798863970497 1103 5 0.04226352022047308 1103 6 0.10641777086738102 1103 7 0.14245029857119107 1103 8 0.11063657675435934 1103 9 0.20788718780431736 1103 10-11 0.23361567558241347 1103 12-13 0.21485840692704983 1103 14-15 0.33447642599999483 1103 16-17 0.3430651373838458 1103 18-19 0.23041686202028444 1103 20-21 0.20040799164005563 1103 22-23 0.22474290868753144 1103 24-25 0.3039115446901519 1103 26-27 0.24980011855826234 1103 28-29 0.30499882122303035 1103 30-31 0.409977786026154 1103 32-33 0.3991748235932988 1103 34-35 0.45812110695653985 1103 36-37 0.5267211352183381 1103 38-39 0.5135336265486075 1103 40-41 0.5759623259537108 1103 42-43 0.5793411648676638 1103 44-45 0.5139633573985805 1103 46-47 0.6355444825084788 1103 48-49 0.6996371815396643 1103 50-51 0.9008203987367551 1103 52-53 0.8968459908470052 1103 54-55 1.0404921049331861 1103 56-57 1.0816964671113212 1103 58-59 1.1977871323449136 1103 60-61 1.2772816791613337 1103 62-63 1.2882721334733986 1103 64-65 1.3332522309603974 1103 66-67 1.4316124060861029 1103 68-69 1.6148176949839517 1103 70-71 1.5573088469316332 1103 72-73 1.7596880750242434 1103 74-75 1.9396168193067105 1103 76-77 1.9948301754659425 1103 78-79 2.004162319368824 1103 80-81 2.105278066300933 1103 82-83 2.2360439782679613 1103 84-85 2.41622572783211 1103 86-87 2.5696187255802805 1103 88-89 2.640634657727194 1103 90-91 2.9088373272642336 1103 92-93 3.113703355563448 1103 94-95 3.189319946905645 1103 96-97 3.607477910689134 1103 98-99 3.8580522221109277 1103 100-101 3.7853606291135797 1104 1 0.19093586632894954 1104 2 0.1596412345478626 1104 3 0.16039038761660862 1104 4 0.14465636669609694 1104 5 0.09973611987569342 1104 6 0.1456086539601955 1104 7 0.1395283600332533 1104 8 0.16569685607868223 1104 9 0.1864289626333857 1104 10-11 0.2051248625740456 1104 12-13 0.2924918805249064 1104 14-15 0.3080257590503095 1104 16-17 0.3231470013817628 1104 18-19 0.2973243304607749 1104 20-21 0.279415785069709 1104 22-23 0.2446700084245066 1104 24-25 0.27121112274922154 1104 26-27 0.38290369343914676 1104 28-29 0.43771994341570775 1104 30-31 0.35000406530649286 1104 32-33 0.37020271778010994 1104 34-35 0.4351251829460665 1104 36-37 0.49941174246716 1104 38-39 0.4682334203109946 1104 40-41 0.5575573879158213 1104 42-43 0.6286235402048206 1104 44-45 0.6796431766131974 1104 46-47 0.8016398604721289 1104 48-49 0.741154094075597 1104 50-51 0.8421013618673641 1104 52-53 0.9007147388212005 1104 54-55 0.9524871120133369 1104 56-57 0.9960918428758703 1104 58-59 1.0626115005091776 1104 60-61 1.1776985286881896 1104 62-63 1.3144524541790972 1104 64-65 1.4801040238507923 1104 66-67 1.4910682619626314 1104 68-69 1.6765896604369992 1104 70-71 1.6377180904306528 1104 72-73 1.9044458199302134 1104 74-75 1.9306625262274792 1104 76-77 1.9707384049872765 1104 78-79 1.933179219953118 1104 80-81 2.1190038152150095 1104 82-83 2.213794554098289 1104 84-85 2.4630366221881594 1104 86-87 2.676527279230264 1104 88-89 2.8832355128914067 1104 90-91 3.0742411829610106 1104 92-93 3.1996585215909406 1104 94-95 3.3805680430083562 1104 96-97 4.020605097242861 1104 98-99 4.225932745803394 1104 100-101 3.8098378401920883 1105 1 0.21384394783710547 1105 2 0.20243562817489646 1105 3 0.20240722407351086 1105 4 0.13299278799585323 1105 5 0.04114621405387453 1105 6 0.05759121686470792 1105 7 0.17947193185136 1105 8 0.19138322277694186 1105 9 0.23387233431234478 1105 10-11 0.20811345972911255 1105 12-13 0.3084626526904799 1105 14-15 0.4311160888132832 1105 16-17 0.3759817457145118 1105 18-19 0.3392945046415363 1105 20-21 0.2736038990601273 1105 22-23 0.2964299723685002 1105 24-25 0.3401766399906023 1105 26-27 0.339896997513101 1105 28-29 0.3892275551165696 1105 30-31 0.3959436290341145 1105 32-33 0.5079400264150209 1105 34-35 0.47686481009900206 1105 36-37 0.44946629973503605 1105 38-39 0.4569768649246413 1105 40-41 0.5183298940604288 1105 42-43 0.5245345979258431 1105 44-45 0.5618996581360349 1105 46-47 0.5589577659153377 1105 48-49 0.6278761111951283 1105 50-51 0.6319331432561484 1105 52-53 0.7925871875999562 1105 54-55 0.903910576813395 1105 56-57 0.960441753332951 1105 58-59 1.106431911340664 1105 60-61 1.073655032370091 1105 62-63 1.1356861782223788 1105 64-65 1.2689563397371515 1105 66-67 1.233241605020595 1105 68-69 1.6680177339661348 1105 70-71 1.7494294592629913 1105 72-73 1.8386577604307242 1105 74-75 1.9021000162481663 1105 76-77 1.8605076235423184 1105 78-79 1.9186544254772215 1105 80-81 2.0930313932836526 1105 82-83 2.178393588034371 1105 84-85 2.428793477672656 1105 86-87 2.67526032103126 1105 88-89 2.8472799070713144 1105 90-91 2.9963427147663246 1105 92-93 3.0058216573312784 1105 94-95 3.314537162754373 1105 96-97 3.481482538221716 1105 98-99 3.4410028254654073 1105 100-101 3.2370945583435393 1106 1 0.13807918888932846 1106 2 0.10233111466111922 1106 3 0.07492199176390102 1106 4 0.1256453267361124 1106 5 -0.10347653320455663 1106 6 0.022271994147054386 1106 7 0.07394521703713508 1106 8 0.09994206497410119 1106 9 0.15321401434771076 1106 10-11 0.10534490910392691 1106 12-13 0.11595267616257132 1106 14-15 0.015412174278573332 1106 16-17 0.05850828400119212 1106 18-19 0.005328963318703472 1106 20-21 -0.08410438615392479 1106 22-23 -0.07078294894440518 1106 24-25 -0.07132186727331202 1106 26-27 -0.123293033015905 1106 28-29 0.023990621870986217 1106 30-31 0.07699884278424918 1106 32-33 0.019303332015589092 1106 34-35 -0.02834133867120414 1106 36-37 0.0953514387342409 1106 38-39 0.05300061887164986 1106 40-41 0.10431257132768224 1106 42-43 0.14786661690035174 1106 44-45 0.09694943031501424 1106 46-47 0.22938175029045027 1106 48-49 0.28198265885892226 1106 50-51 0.3663783557477558 1106 52-53 0.29925670355643064 1106 54-55 0.29392408127443304 1106 56-57 0.31251911385473363 1106 58-59 0.5461180755397521 1106 60-61 0.5065005452307432 1106 62-63 0.49858202760565007 1106 64-65 0.5983579742187786 1106 66-67 0.9073624591625702 1106 68-69 0.9119653838150796 1106 70-71 0.8136517675269772 1106 72-73 1.142926470517022 1106 74-75 1.2994663741207795 1106 76-77 1.2564040703550745 1106 78-79 1.4809547195431954 1106 80-81 1.2828518901196624 1106 82-83 1.7189351359777199 1106 84-85 1.8982782434302514 1106 86-87 1.8065986384579205 1106 88-89 1.9039437795912129 1106 90-91 2.0172891829487547 1106 92-93 2.1077473046879014 1106 94-95 2.4509015939011967 1106 96-97 2.894292437751794 1106 98-99 3.185630776412115 1106 100-101 3.178073739611765 1107 1 0.2254618439571061 1107 2 0.22658969386828431 1107 3 0.17181781514821637 1107 4 0.1044929324962709 1107 5 0.05633183692181376 1107 6 0.086112627096675 1107 7 0.10335369640127112 1107 8 0.12676370461100817 1107 9 0.15928741463093132 1107 10-11 0.18576810813271294 1107 12-13 0.21635959512030212 1107 14-15 0.3723938826466622 1107 16-17 0.3359108704061242 1107 18-19 0.32055533301710426 1107 20-21 0.30035431597677587 1107 22-23 0.3531317362780442 1107 24-25 0.38435039582738284 1107 26-27 0.36918537953327046 1107 28-29 0.42510394228392556 1107 30-31 0.45091452700635415 1107 32-33 0.5043795194560872 1107 34-35 0.47678544473728124 1107 36-37 0.5224487401618418 1107 38-39 0.48090157152392976 1107 40-41 0.531368152873334 1107 42-43 0.5922825866914323 1107 44-45 0.671205851061778 1107 46-47 0.7142279607638429 1107 48-49 0.8000577200696526 1107 50-51 0.6701286522125542 1107 52-53 0.7693825764776747 1107 54-55 0.9756233807220482 1107 56-57 0.970979610872071 1107 58-59 0.9844319690989778 1107 60-61 1.1111247955798476 1107 62-63 1.2613439376225912 1107 64-65 1.3572413845406501 1107 66-67 1.6993836197351442 1107 68-69 1.7272259679576045 1107 70-71 1.7853891355904352 1107 72-73 1.925104741125427 1107 74-75 1.9682481939420349 1107 76-77 2.0484396206636113 1107 78-79 1.9475621467381181 1107 80-81 2.0373039346682127 1107 82-83 2.355413292617346 1107 84-85 2.4514220228768018 1107 86-87 2.5012921282825644 1107 88-89 2.629982255260604 1107 90-91 2.627085559903108 1107 92-93 2.8349872715480267 1107 94-95 2.8557172173862 1107 96-97 3.099419831947639 1107 98-99 3.2446575768294785 1107 100-101 3.1878414716744707 1108 1 0.23808208660913266 1108 2 0.24235680087738842 1108 3 0.13870773850112528 1108 4 0.104377098267463 1108 5 0.12570878322127044 1108 6 0.1271661394076773 1108 7 0.16498908954988423 1108 8 0.14916480338141014 1108 9 0.1303276163479623 1108 10-11 0.13848111124885776 1108 12-13 0.17246317987941495 1108 14-15 0.27547140160158534 1108 16-17 0.24063592441900283 1108 18-19 0.27857918038896656 1108 20-21 0.2817069184719756 1108 22-23 0.2676177374012241 1108 24-25 0.32715967580388394 1108 26-27 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0.15783758883768684 2316 56-57 0.22038749181581352 2316 58-59 0.4454581007161096 2316 60-61 0.45491456820195 2316 62-63 0.45023264665706364 2316 64-65 0.5992578940924389 2316 66-67 0.7050872940138646 2316 68-69 1.0696626243268774 2316 70-71 1.0387330660496623 2316 72-73 1.124709099257995 2316 74-75 1.5372271499676522 2316 76-77 1.52364439073337 2316 78-79 1.6564767207318774 2316 80-81 1.6411002889295538 2316 82-83 1.8047617777134342 2316 84-85 2.068795312186932 2316 86-87 2.1215462207602513 2316 88-89 2.4644166203705744 2316 90-91 2.678901873151961 2316 92-93 2.782266157164514 2316 94-95 3.3082426716707225 2316 96-97 3.64232490926641 2316 98-99 3.8505695354387264 2316 100-101 4.239692017502094 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 24.0 5 47.0 6 127.0 7 324.0 8 850.0 9 1632.0 10 2950.0 11 4810.0 12 7586.0 13 11418.0 14 15233.0 15 20885.0 16 27378.0 17 38064.0 18 39329.0 19 32541.0 20 30680.0 21 31871.0 22 34455.0 23 37914.0 24 42215.0 25 47651.0 26 53299.0 27 62777.0 28 74771.0 29 89903.0 30 107719.0 31 129633.0 32 157411.0 33 197420.0 34 258257.0 35 354125.0 36 484877.0 37 572031.0 38 289243.0 39 12537.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32662488787422 18.587375591955364 14.911942102601012 23.174057417569404 2 15.887984447548686 19.72006810310835 39.60085709657009 24.79109035277288 3 19.36142263603572 23.414758039523235 29.91914784629594 27.304671478145103 4 13.075708790948504 16.54777204384548 36.14642055413523 34.23009861107079 5 13.60089274868127 37.15267758817102 34.19093426810428 15.055495395043433 6 33.00865863710589 35.97947795120589 16.37806071101008 14.63380270067815 7 29.05456762617302 32.97401126835462 19.710299068305424 18.261122037166928 8 27.1139136207101 34.35458809751136 19.03925075519837 19.49224752658017 9 26.450376605098725 16.483246459728697 19.827872597604827 37.238504337567754 10-11 25.33452832293448 26.244309664287385 26.520777079427077 21.90038493335106 12-13 26.43825861592087 23.589308663368985 27.13618144282005 22.836251277890092 14-15 22.76079272737275 24.970728867250713 25.74126794203536 26.527210463341174 16-17 22.175434585846645 28.255747373658487 26.83399987377729 22.73481816671758 18-19 22.273444794383852 26.276705801812046 29.509641671212822 21.94020773259128 20-21 22.320572005764067 25.40764885031915 30.304829316670716 21.96694982724607 22-23 22.360330643173498 25.34197497453 30.19159767315666 22.106096709139845 24-25 22.264476636310928 25.593685045044605 30.07380242966107 22.068035888983395 26-27 21.509036974505076 26.08893521732705 29.870207868217225 22.531819939950644 28-29 21.883988209028438 26.496327164314128 29.686261745510002 21.933422881147436 30-31 21.84408900380349 25.872655673373586 29.61712961052813 22.666125712294793 32-33 21.465726567430572 25.96819371667744 29.680072860747035 22.88600685514495 34-35 22.501662776665864 26.134126779384992 29.375104687685305 21.98910575626384 36-37 22.69187429113871 25.447826128149593 29.362574202170983 22.497725378540714 38-39 21.99758479059933 25.898306908256885 29.26653373132431 22.83757456981948 40-41 22.60064985527951 25.754469875535335 29.347930242570154 22.296950026614997 42-43 22.187161788990867 25.828279812006254 29.1617700411179 22.822788357884978 44-45 22.167640953769844 26.157259153339847 29.43106553893565 22.24403435395466 46-47 22.793066615321845 26.579197095350082 28.321146578769223 22.30658971055885 48-49 22.38213994825787 26.640383007920242 28.24561162226715 22.73186542155474 50-51 22.69551145402117 26.90283756546083 28.21556878907211 22.18608219144589 52-53 23.18884961621274 26.334927162174758 28.19718550914656 22.279037712465943 54-55 22.355703477541987 26.8456858888843 28.412039138201617 22.386571495372092 56-57 22.487274583243554 26.952165269201206 28.096467140078147 22.46409300747709 58-59 22.47537053082294 26.36692598857883 28.061992759768888 23.09571072082934 60-61 21.924024945025895 26.86883690225688 28.376831260438966 22.83030689227826 62-63 22.18441654097882 26.838671819486276 28.53890363524394 22.43800800429096 64-65 21.788373759739855 26.97326860218307 28.290584184878032 22.94777345319904 66-67 21.664807962608723 26.901539826032632 28.344727510113866 23.088924701244775 68-69 22.485044159994327 26.432716599133364 28.514950949979305 22.567288290892996 70-71 22.191552859952417 26.750514594307145 27.813413529054902 23.244519016685537 72-73 22.077677380313844 26.93190240205135 27.81654617442875 23.173874043206055 74-75 22.053701182306206 27.316484285581122 27.76480404157097 22.865010490541703 76-77 22.171473367165902 26.69735131013342 28.171024099975707 22.960151222724974 78-79 22.265819477108003 27.01940253576182 28.012512244059053 22.702265743071123 80-81 22.485301771733617 27.073056085388934 27.680453472852783 22.76118867002466 82-83 22.246911995501133 27.167272577236407 27.827631050127827 22.758184377134633 84-85 22.157501945377824 27.340627252160697 27.73780219068187 22.764068611779606 86-87 22.469511920238237 27.307597267453847 27.28030420305551 22.94258660925241 88-89 22.164703796919007 27.567401539427784 27.034622607919633 23.233272055733583 90-91 22.079286704205387 27.36955977249378 27.33551238641766 23.215641136883175 92-93 22.161892056681015 26.896755649076482 27.60380135055219 23.337550943690317 94-95 21.90914775516092 26.627982814149416 27.78849838966697 23.67437104102269 96-97 22.166888931981923 26.292695531970523 27.7602884238203 23.78012711222725 98-99 21.84204132341278 26.65836897672521 27.302517271572846 24.19707242828916 100-101 22.07180940068674 25.904089095490672 27.775393911054262 24.248707592768326 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1227.0 1 847.0 2 735.0 3 1032.5 4 843.5 5 779.0 6 1544.0 7 3501.5 8 5797.0 9 6523.0 10 5200.5 11 3879.5 12 3682.0 13 3844.0 14 4202.0 15 4526.5 16 4780.5 17 5057.0 18 5406.0 19 6006.0 20 6840.0 21 7995.0 22 9445.5 23 11346.0 24 13979.0 25 17501.0 26 21950.0 27 27018.5 28 31251.0 29 35109.5 30 39296.0 31 46273.0 32 58137.5 33 61714.0 34 58290.0 35 60229.0 36 65969.5 37 71636.0 38 75505.0 39 78804.0 40 83557.0 41 90670.0 42 95060.5 43 98525.0 44 102773.5 45 108970.5 46 111906.0 47 115515.0 48 117808.5 49 114662.0 50 114992.0 51 114112.0 52 112725.0 53 115091.5 54 119317.5 55 114005.5 56 104432.5 57 97285.5 58 91318.0 59 81403.5 60 64885.5 61 54122.0 62 47372.5 63 35371.0 64 24512.5 65 19049.0 66 14198.0 67 10861.5 68 8406.5 69 6234.0 70 4778.0 71 3272.5 72 2190.0 73 1671.0 74 1272.5 75 901.0 76 611.0 77 390.0 78 250.0 79 173.5 80 106.0 81 52.5 82 29.5 83 18.0 84 17.5 85 14.5 86 7.0 87 2.0 88 2.0 89 2.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014822761037226526 3 0.0029645522074453045 4 0.0015281196945594357 5 0.11876546266115931 6 6.112478778237741E-5 7 0.0 8 2.1393675723832095E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.5281196945594353E-5 18-19 0.0 20-21 0.0 22-23 2.5978034807510404E-4 24-25 4.889983022590193E-4 26-27 0.0056998864607066945 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010971899406936747 36-37 6.723726656061515E-4 38-39 0.006998788201082215 40-41 0.010498182301623322 42-43 0.005623480475978722 44-45 0.003529956494432296 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.112478778237741E-5 68-69 8.863094228444725E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.112478778237741E-5 82-83 0.0018337436334713224 84-85 0.004187047963092853 86-87 0.003102082979955654 88-89 0.0 90-91 0.002582522283805446 92-93 0.0 94-95 3.0562393891188705E-5 96-97 1.375307725103492E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3271995.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.358462527634124 #Duplication Level Percentage of deduplicated Percentage of total 1 69.83576215461468 30.978070386242447 2 15.425606207862838 13.685123498750482 3 5.911780877671439 7.867125316013175 4 2.8522918472887526 5.060931241033377 5 1.5912883255340553 3.5293551779432026 6 0.993665229600134 2.6446477111338305 7 0.675406553305133 2.097199739999298 8 0.4557232694764644 1.6172146857634124 9 0.33038485501352577 1.3189827789733783 >10 1.7354046470561255 13.571357540529402 >50 0.11279578924558072 3.45013577363974 >100 0.067993092176615 5.712598846164737 >500 0.006670657006573746 1.998240754601945 >1k 0.004676336869340714 4.335058321309886 >5k 4.126179590594747E-4 1.1817395401362594 >10k+ 1.3753931968649158E-4 0.9522186877656454 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17096 0.5224946859637621 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14119 0.4315104393496934 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9167 0.2801654648005269 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6322 0.19321545418009503 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6253 0.191106649001603 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5823 0.17796481962839186 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5754 0.17585601444989982 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5420 0.1656481748902428 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4657 0.14232906835126583 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4519 0.1381114579942818 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4229 0.12924836376583704 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3959 0.1209965174152161 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3611 0.11036080434108242 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3391 0.10363707768502092 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3380 0.10330089135221784 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3375 0.10314807938276188 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.056239389118871E-5 2 0.0 0.0 0.0 0.0 3.056239389118871E-5 3 0.0 0.0 0.0 0.0 3.056239389118871E-5 4 0.0 0.0 0.0 0.0 3.056239389118871E-5 5 0.0 0.0 0.0 0.0 3.056239389118871E-5 6 0.0 0.0 0.0 0.0 6.112478778237742E-5 7 0.0 0.0 0.0 0.0 6.112478778237742E-5 8 0.0 0.0 0.0 0.0 6.112478778237742E-5 9 0.0 0.0 0.0 0.0 6.112478778237742E-5 10-11 0.0 0.0 0.0 0.0 9.168718167356613E-5 12-13 0.0 0.0 0.0 0.0 1.8337436334713226E-4 14-15 0.0 0.0 0.0 0.0 2.5978034807510404E-4 16-17 0.0 0.0 0.0 0.0 3.361863328030758E-4 18-19 0.0 0.0 0.0 0.0 3.361863328030758E-4 20-21 0.0 0.0 0.0 1.5281196945594356E-5 3.667487266942645E-4 22-23 0.0 0.0 0.0 3.056239389118871E-4 3.973111205854532E-4 24-25 0.0 3.056239389118871E-5 0.0 6.418102717149628E-4 3.973111205854532E-4 26-27 0.0 3.056239389118871E-5 0.0 0.001375307725103492 4.1259231753104755E-4 28-29 0.0 3.056239389118871E-5 0.0 0.004294016341712013 4.278735144766419E-4 30-31 0.0 3.056239389118871E-5 0.0 0.01382948323576289 4.431547114222363E-4 32-33 0.0 3.056239389118871E-5 0.0 0.03371032046198115 4.889983022590194E-4 34-35 0.0 3.056239389118871E-5 0.0 0.061919410023548324 4.889983022590194E-4 36-37 0.0 3.056239389118871E-5 0.0 0.10808390599618886 4.889983022590194E-4 38-39 0.0 3.056239389118871E-5 0.0 0.1805779043060885 4.889983022590194E-4 40-41 0.0 3.056239389118871E-5 0.0 0.25482924026473147 4.889983022590194E-4 42-43 0.0 3.056239389118871E-5 0.0 0.3286374215119522 4.889983022590194E-4 44-45 0.0 3.056239389118871E-5 0.0 0.4202634783977359 5.042794992046137E-4 46-47 0.0 3.056239389118871E-5 0.0 0.5255814877467722 5.501230900413968E-4 48-49 0.0 3.056239389118871E-5 0.0 0.6324428979873136 6.112478778237742E-4 50-51 0.0 3.056239389118871E-5 0.0 0.7528892923124884 6.112478778237742E-4 52-53 0.0 3.056239389118871E-5 0.0 0.8660312744976688 6.876538625517459E-4 54-55 0.0 3.056239389118871E-5 0.0 0.9864318252320068 7.487786503341234E-4 56-57 0.0 3.056239389118871E-5 0.0 1.1233055062736954 7.946222411709064E-4 58-59 0.0 3.056239389118871E-5 0.0 1.266093010533329 8.251846350620951E-4 60-61 0.0 3.056239389118871E-5 0.0 1.422052906560065 8.251846350620951E-4 62-63 0.0 3.056239389118871E-5 0.0 1.5760720905747105 8.251846350620951E-4 64-65 0.0 3.056239389118871E-5 0.0 1.7314513011175139 8.251846350620951E-4 66-67 0.0 3.056239389118871E-5 0.0 1.9075212523246523 8.251846350620951E-4 68-69 0.0 3.056239389118871E-5 0.0 2.103945757863322 8.251846350620951E-4 70-71 0.0 3.056239389118871E-5 0.0 2.3059173378932423 8.251846350620951E-4 72-73 0.0 3.056239389118871E-5 0.0 2.5129164317182635 8.710282258988782E-4 74-75 0.0 3.056239389118871E-5 0.0 2.721657581995083 8.863094228444726E-4 76-77 0.0 3.056239389118871E-5 0.0 2.9446408078251953 8.863094228444726E-4 78-79 0.0 3.056239389118871E-5 0.0 3.1915543880721087 9.168718167356613E-4 80-81 0.0 3.056239389118871E-5 0.0 3.4482937779550396 9.627154075724444E-4 82-83 0.0 3.056239389118871E-5 0.0 3.7126890475077134 9.779966045180388E-4 84-85 0.0 3.056239389118871E-5 0.0 3.9806753983426013 9.779966045180388E-4 86-87 0.0 3.056239389118871E-5 0.0 4.25729562545175 0.0010085589984092274 88-89 0.0 3.056239389118871E-5 0.0 4.551886540168918 0.0010391213923004162 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7195 0.0 56.449 1 GTATCAA 12835 0.0 41.9079 1 TATCAAC 16385 0.0 32.653915 2 ATCAACG 16540 0.0 32.114437 3 TCAACGC 16880 0.0 31.522482 4 CAACGCA 17005 0.0 31.233883 5 AACGCAG 17555 0.0 30.301811 6 TAAGGTG 2355 0.0 27.863625 5 TGGTATC 3005 0.0 27.22157 2 ACGCAGA 19860 0.0 26.641418 7 CGCAGAG 19940 0.0 26.486897 8 AGGTAAG 2150 0.0 26.323141 2 GTGGTAT 3315 0.0 25.967148 1 GTAAGGT 2465 0.0 25.077785 4 AAGGTAA 2225 0.0 24.36711 1 GCAGAGT 21845 0.0 24.068386 9 GTACATG 11155 0.0 23.150448 1 TACATGG 10985 0.0 23.119072 2 GAGTACT 15120 0.0 22.601143 12-13 GGTAAGG 2675 0.0 22.221272 3 >>END_MODULE