FastQCFastQC Report
Thu 26 May 2016
SRR937982_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937982_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3267627
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176830.5411572373468575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136160.4166938270494153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88370.27044090405667476No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA62190.19032160035401838No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA61910.18946470940532686No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA56730.1736122268545339No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA54250.1660226213089805No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC48770.1492520413131609No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG45380.13887754018436008No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA43520.13318533602519506No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA41070.1256875402241443No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA38930.11913844511628774No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG34480.1055199996817262No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT33910.10377561453617565No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33890.10371440803984054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74000.058.5644571
GTATCAA135450.042.6017341
TATCAAC169950.033.893362
ATCAACG169550.033.8622363
TCAACGC172900.033.2341424
CAACGCA174000.032.969415
AACGCAG180050.031.9358796
ACGCAGA203650.027.9947787
CGCAGAG206250.027.618848
TGGTATC30750.026.4292092
GTGGTAT34650.025.0696411
GCAGAGT222800.024.9903689
GTGTAAG15200.024.8063851
TAAGGTA13100.024.6712274
AGGTAAG21900.024.5226572
TAAGGTG24550.024.1997935
GAGTACT151400.023.98806612-13
GTACATG114200.023.321051
CAGAGTA210600.022.88340810-11
GTACTTT158750.022.69806514-15
Produced by FastQC (version 0.11.5)