##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937982_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3267627 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.86697441293024 33.0 31.0 34.0 30.0 34.0 2 32.06060606060606 34.0 31.0 34.0 30.0 34.0 3 32.13518250400061 34.0 31.0 34.0 30.0 34.0 4 35.65871716692266 37.0 35.0 37.0 35.0 37.0 5 35.67033783231685 37.0 35.0 37.0 35.0 37.0 6 35.69889678350681 37.0 35.0 37.0 35.0 37.0 7 35.64206624562718 37.0 35.0 37.0 33.0 37.0 8 35.61088582019919 37.0 35.0 37.0 33.0 37.0 9 37.25086370017141 39.0 37.0 39.0 34.0 39.0 10-11 37.161671604500754 39.0 37.0 39.0 34.0 39.0 12-13 37.06135721733233 39.0 37.0 39.0 33.0 39.0 14-15 38.25298006779843 40.0 38.0 41.0 33.0 41.0 16-17 38.11125887379435 40.0 38.0 41.0 33.0 41.0 18-19 38.25107776989234 40.0 38.0 41.0 33.5 41.0 20-21 38.22351464839775 40.0 38.0 41.0 34.0 41.0 22-23 38.143281500611906 40.0 38.0 41.0 33.0 41.0 24-25 38.03395568098807 40.0 38.0 41.0 33.0 41.0 26-27 37.84635164907133 40.0 38.0 41.0 33.0 41.0 28-29 37.63419830353954 40.0 37.5 41.0 32.0 41.0 30-31 37.39531424486332 40.0 37.0 41.0 32.0 41.0 32-33 37.12021812771164 40.0 36.5 41.0 31.0 41.0 34-35 36.87258475340056 39.5 36.0 41.0 30.5 41.0 36-37 36.59132743731154 39.0 35.5 41.0 30.0 41.0 38-39 36.27475030044739 39.0 35.0 41.0 30.0 41.0 40-41 36.04673789266646 39.0 35.0 40.0 29.0 41.0 42-43 35.85033542690154 39.0 35.0 40.5 28.5 41.0 44-45 35.56871653343542 39.0 35.0 40.0 27.0 41.0 46-47 35.16011053281173 38.5 35.0 40.0 25.5 41.0 48-49 34.73139697401203 38.0 34.0 40.0 24.0 41.0 50-51 33.994357832151586 37.5 33.5 39.5 23.0 40.5 52-53 34.09849533009734 38.0 33.5 40.0 23.0 40.5 54-55 34.191789179119894 38.0 34.0 40.0 22.5 41.0 56-57 33.882418189101756 38.0 34.0 40.0 21.5 41.0 58-59 33.543361436296124 37.5 33.0 40.0 19.5 41.0 60-61 33.11566512946551 37.0 33.0 40.0 18.0 41.0 62-63 32.57290382286595 36.0 32.0 39.0 13.5 41.0 64-65 32.051768454600236 35.5 31.5 39.0 8.0 40.5 66-67 31.518836299247127 35.0 31.0 38.5 6.5 40.0 68-69 30.92482633421746 35.0 30.5 38.0 2.0 40.0 70-71 30.378867447233112 35.0 29.5 37.0 2.0 39.5 72-73 29.764068695723225 34.0 29.0 37.0 2.0 39.0 74-75 29.10926124677021 34.0 28.5 36.0 2.0 39.0 76-77 28.45972061682683 34.0 27.0 35.5 2.0 38.0 78-79 27.74731142814036 33.5 26.0 35.0 2.0 37.0 80-81 27.11037153261373 33.0 25.0 35.0 2.0 36.5 82-83 26.387885153354407 32.5 23.5 35.0 2.0 36.0 84-85 25.74183696609191 32.0 21.0 35.0 2.0 36.0 86-87 25.079481378994604 31.5 19.0 34.5 2.0 35.0 88-89 24.26894991380595 31.0 13.5 34.0 2.0 35.0 90-91 23.456054041663876 30.5 4.5 34.0 2.0 35.0 92-93 22.575776702787678 30.0 2.0 34.0 2.0 35.0 94-95 21.638146734618118 29.0 2.0 34.0 2.0 35.0 96-97 20.45697902483974 29.0 2.0 34.0 2.0 35.0 98-99 18.974201614810994 26.0 2.0 33.5 2.0 35.0 100-101 15.353973081994976 11.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.43435307501299647 1101 2 0.34947391702397823 1101 3 0.20385421552610694 1101 4 0.18268156785831735 1101 5 0.26884496189171614 1101 6 0.28682478817274415 1101 7 0.2879184143100275 1101 8 0.29350185356788216 1101 9 0.3860657024614085 1101 10-11 0.4136669631805674 1101 12-13 0.4784878246471749 1101 14-15 0.5962983330004192 1101 16-17 0.6128717907330739 1101 18-19 0.5230528555248739 1101 20-21 0.4995694900377359 1101 22-23 0.4854470185844093 1101 24-25 0.5639469767558296 1101 26-27 0.6895167557575164 1101 28-29 0.6982699747710228 1101 30-31 0.7850345452331098 1101 32-33 0.6807593235162201 1101 34-35 0.714246864792166 1101 36-37 0.7920846152736445 1101 38-39 0.9032603585163983 1101 40-41 0.9030617889742274 1101 42-43 1.0781409282823944 1101 44-45 1.0326126085375051 1101 46-47 1.0237836092024821 1101 48-49 1.0830741541217108 1101 50-51 1.1653841000426013 1101 52-53 1.1328697581745644 1101 54-55 1.170527719915306 1101 56-57 1.1691067047720196 1101 58-59 1.255385882123079 1101 60-61 1.167834402761919 1101 62-63 1.1895182209982949 1101 64-65 1.3517276320299274 1101 66-67 1.3475933176191681 1101 68-69 1.4927961874710896 1101 70-71 1.7962469249035387 1101 72-73 1.9308470715599704 1101 74-75 2.0293525826941767 1101 76-77 2.0927987126315557 1101 78-79 2.1524849623347713 1101 80-81 2.3072587344958677 1101 82-83 2.421774917631854 1101 84-85 2.5885532462562146 1101 86-87 2.798578522395381 1101 88-89 3.1501667876611883 1101 90-91 3.4660151982619816 1101 92-93 3.813819484235829 1101 94-95 3.9140927170118935 1101 96-97 4.0809828302997815 1101 98-99 4.227542514751171 1101 100-101 4.103469104772826 1102 1 0.406716010674959 1102 2 0.2993901414653948 1102 3 0.17649389498289025 1102 4 0.14638934745427434 1102 5 0.21612477959806142 1102 6 0.3072838867978902 1102 7 0.09369957016193098 1102 8 0.1586405160340476 1102 9 0.34047980880956885 1102 10-11 0.2817509528424367 1102 12-13 0.3268791442880925 1102 14-15 0.4188465291802004 1102 16-17 0.4421133451229551 1102 18-19 0.383523454555295 1102 20-21 0.3475447152285156 1102 22-23 0.4561854608300493 1102 24-25 0.3665979425145949 1102 26-27 0.3752778470725744 1102 28-29 0.34530693817465163 1102 30-31 0.30146587160299276 1102 32-33 0.550448659643088 1102 34-35 0.45300940895096886 1102 36-37 0.5157207008537839 1102 38-39 0.44933128787760523 1102 40-41 0.6834621621256787 1102 42-43 0.7698655302796737 1102 44-45 0.7084881839400978 1102 46-47 0.7540627693058681 1102 48-49 0.8452706091829683 1102 50-51 0.8685197344765285 1102 52-53 0.8430230255436868 1102 54-55 0.8852022370359265 1102 56-57 0.928059127962463 1102 58-59 0.8867575807268224 1102 60-61 0.9598668241171922 1102 62-63 0.847118123122506 1102 64-65 1.0111931078744831 1102 66-67 1.0094726551224191 1102 68-69 1.1396593601759726 1102 70-71 1.1932897302683507 1102 72-73 1.4385645163901408 1102 74-75 1.3674763649817194 1102 76-77 1.5106349236150507 1102 78-79 1.7916173546331713 1102 80-81 1.8595377416683299 1102 82-83 2.0867889108111406 1102 84-85 2.08975454922766 1102 86-87 2.216986205644858 1102 88-89 2.5767866166844158 1102 90-91 2.7591813882939427 1102 92-93 2.9347164546742803 1102 94-95 3.266001570182919 1102 96-97 3.5459527793737884 1102 98-99 3.8141173820835945 1102 100-101 3.881126753612845 1103 1 0.536966393375149 1103 2 0.47680847236470214 1103 3 0.5092197408822798 1103 4 0.3266836131097932 1103 5 0.42197419806971936 1103 6 0.41353201728546196 1103 7 0.47090422238863283 1103 8 0.45560981204165074 1103 9 0.5045667301404819 1103 10-11 0.5979568895341529 1103 12-13 0.602368384694195 1103 14-15 0.8938841305737597 1103 16-17 0.8844237633658736 1103 18-19 0.8629796859701386 1103 20-21 0.866077187559732 1103 22-23 0.8877542324944017 1103 24-25 0.8253390260400124 1103 26-27 0.8745950747396023 1103 28-29 0.9421485618791863 1103 30-31 0.9126738669578032 1103 32-33 0.9076064376945681 1103 34-35 0.9122114922476428 1103 36-37 0.9009373883416956 1103 38-39 0.8939162297247734 1103 40-41 0.853914188237269 1103 42-43 0.9952106642687255 1103 44-45 0.9546213108206061 1103 46-47 1.1088508677697888 1103 48-49 1.0264029090791524 1103 50-51 0.91589551967175 1103 52-53 1.120944937109158 1103 54-55 1.2379296313314967 1103 56-57 1.1672145482288627 1103 58-59 1.1535795327617322 1103 60-61 1.2219790476492918 1103 62-63 1.285796355839416 1103 64-65 1.4656449932227886 1103 66-67 1.4152031989965757 1103 68-69 1.6077273488445378 1103 70-71 1.704061570572403 1103 72-73 1.8643278581106166 1103 74-75 1.7983304110465035 1103 76-77 1.9192122642446172 1103 78-79 2.1038951509838846 1103 80-81 2.3030549939689315 1103 82-83 2.553345312901474 1103 84-85 2.5640591961757373 1103 86-87 2.5400164873473905 1103 88-89 2.89591867024485 1103 90-91 3.1540053813965443 1103 92-93 3.304140838395007 1103 94-95 3.7789940921331926 1103 96-97 4.111368295007306 1103 98-99 4.433003099888392 1103 100-101 4.373591835519482 1104 1 0.2067017375085065 1104 2 0.1562622319353153 1104 3 0.21634435611596103 1104 4 0.22571400709415457 1104 5 0.19388224556204392 1104 6 0.1912348090640279 1104 7 0.15864489295508122 1104 8 0.25943992314867614 1104 9 0.24847574644680748 1104 10-11 0.1920462426975078 1104 12-13 0.23192196378743546 1104 14-15 0.37132369211388294 1104 16-17 0.3745123899187348 1104 18-19 0.31612237857813597 1104 20-21 0.34882721413058704 1104 22-23 0.3749448481818263 1104 24-25 0.3762989745743255 1104 26-27 0.4452776823821907 1104 28-29 0.48785195354574995 1104 30-31 0.4110655641809444 1104 32-33 0.37720102020522717 1104 34-35 0.33681287842834706 1104 36-37 0.43079069426617167 1104 38-39 0.4957282465952133 1104 40-41 0.6211666526834279 1104 42-43 0.5371418258440173 1104 44-45 0.5765159068210792 1104 46-47 0.5012791724679175 1104 48-49 0.5319901963590752 1104 50-51 0.3814446905590927 1104 52-53 0.26824656528896895 1104 54-55 0.39629616545050794 1104 56-57 0.6190225666976445 1104 58-59 0.6356477871387654 1104 60-61 0.6139290770816217 1104 62-63 0.7925397305006356 1104 64-65 0.8736219067326303 1104 66-67 0.8420397939015096 1104 68-69 0.9752553197719358 1104 70-71 1.2033834390957558 1104 72-73 1.285827417136737 1104 74-75 1.3615752890045556 1104 76-77 1.3751950356045128 1104 78-79 1.5584373480455547 1104 80-81 1.7171111386819469 1104 82-83 1.8711460693530775 1104 84-85 1.9674739717133853 1104 86-87 2.0683976022192994 1104 88-89 2.14816831409329 1104 90-91 2.364982003138035 1104 92-93 2.6272033892373017 1104 94-95 2.8007901077323254 1104 96-97 2.9293325004980737 1104 98-99 3.0471042617498227 1104 100-101 3.1554919270867128 1105 1 0.3367458342743852 1105 2 0.31828862086304355 1105 3 0.19964727922587144 1105 4 0.19019104057879588 1105 5 0.2313875081230634 1105 6 0.20091394042687227 1105 7 0.2693596613713254 1105 8 0.2688906865501437 1105 9 0.3077796630350207 1105 10-11 0.29953400287386955 1105 12-13 0.29814925515699287 1105 14-15 0.34265555115337776 1105 16-17 0.4347760442255719 1105 18-19 0.3820578988065577 1105 20-21 0.3824414827783045 1105 22-23 0.3653704340155599 1105 24-25 0.36744573516702417 1105 26-27 0.27823715056447895 1105 28-29 0.3711625277591324 1105 30-31 0.41620520264494587 1105 32-33 0.5077124848206225 1105 34-35 0.4987022659982969 1105 36-37 0.5520923858202806 1105 38-39 0.546497056269942 1105 40-41 0.7100599982429969 1105 42-43 0.6938453090768348 1105 44-45 0.6544794374674368 1105 46-47 0.652911712106075 1105 48-49 0.6886612983354681 1105 50-51 0.6492554010941589 1105 52-53 0.6676069796447734 1105 54-55 0.7864652062895345 1105 56-57 0.8140543401379787 1105 58-59 0.8297764779223371 1105 60-61 0.9314266652363159 1105 62-63 0.9563273296542789 1105 64-65 1.0271063920813575 1105 66-67 1.1675377855839422 1105 68-69 1.2191352137594897 1105 70-71 1.4090892300878046 1105 72-73 1.3968629396177725 1105 74-75 1.4952399694913687 1105 76-77 1.446438703054156 1105 78-79 1.6641957452287066 1105 80-81 1.8228239011109117 1105 82-83 1.9539709150105153 1105 84-85 2.0252758737699423 1105 86-87 2.1999570641098103 1105 88-89 2.2997078850566908 1105 90-91 2.5439137382063457 1105 92-93 2.805823351548824 1105 94-95 3.170447346064588 1105 96-97 3.140212204567373 1105 98-99 3.346547430363053 1105 100-101 3.5979364041551545 1106 1 0.11394921225598509 1106 2 0.12286576728885024 1106 3 0.10369284096444886 1106 4 0.16436299699314105 1106 5 0.24230396273376442 1106 6 -0.013348524269304107 1106 7 0.15281155962174608 1106 8 0.1148565898153393 1106 9 0.06280655452761863 1106 10-11 0.07001972754599706 1106 12-13 0.2577552971207666 1106 14-15 0.3176620759522706 1106 16-17 0.2283570868884297 1106 18-19 0.23868550989126192 1106 20-21 0.10065802221139108 1106 22-23 0.08265318151516254 1106 24-25 0.21666513619048544 1106 26-27 0.16927389450341224 1106 28-29 0.14532670102050105 1106 30-31 0.10922212983750512 1106 32-33 0.15844470707391878 1106 34-35 0.21456540368087218 1106 36-37 0.22179448214495778 1106 38-39 0.043827994069964404 1106 40-41 0.27776387490565924 1106 42-43 0.16176935109653812 1106 44-45 0.27578989672006315 1106 46-47 0.526317051255802 1106 48-49 0.5318134286823053 1106 50-51 0.5563247410641452 1106 52-53 0.5440546460970523 1106 54-55 0.15197167050101257 1106 56-57 0.4172422636673403 1106 58-59 0.6578195043104813 1106 60-61 0.7276033195058673 1106 62-63 0.4334488214097263 1106 64-65 0.5379085228942486 1106 66-67 0.8104173191540376 1106 68-69 0.6241442086608266 1106 70-71 0.7574175300048474 1106 72-73 1.0701771646114828 1106 74-75 1.005123268802535 1106 76-77 1.2539260962105736 1106 78-79 1.565691135924343 1106 80-81 1.6984810881768944 1106 82-83 1.7960576855146932 1106 84-85 1.8628085676729818 1106 86-87 1.5613142688859654 1106 88-89 1.965220081770056 1106 90-91 2.1335173566733907 1106 92-93 2.467190762974674 1106 94-95 2.3602598047020216 1106 96-97 2.5211569954475657 1106 98-99 2.8419843122548727 1106 100-101 3.2194060685008576 1107 1 0.15402884455783905 1107 2 0.10802690244998558 1107 3 0.011114143941306054 1107 4 0.013193631379330384 1107 5 0.14067657777304277 1107 6 0.17361315602570926 1107 7 0.05079723649631518 1107 8 0.17319553482094818 1107 9 0.1679933430477405 1107 10-11 0.175300578382398 1107 12-13 0.17484658032316247 1107 14-15 0.19685746903655144 1107 16-17 0.3139973519731427 1107 18-19 0.2255055622668678 1107 20-21 0.350178357842843 1107 22-23 0.3065634482032067 1107 24-25 0.3395241496050545 1107 26-27 0.38820313398820616 1107 28-29 0.5193107211156374 1107 30-31 0.6145989135476242 1107 32-33 0.48215348800491853 1107 34-35 0.348614091618451 1107 36-37 0.3505396053345251 1107 38-39 0.32386994800081226 1107 40-41 0.42596145921434925 1107 42-43 0.5459944058771455 1107 44-45 0.49319676968249127 1107 46-47 0.4924591601756916 1107 48-49 0.5689370458059173 1107 50-51 0.36930694404635034 1107 52-53 0.404663646150496 1107 54-55 0.41280968022790887 1107 56-57 0.5684757626786094 1107 58-59 0.4474763906340371 1107 60-61 0.5323176586646454 1107 62-63 0.621270156453285 1107 64-65 0.8289648533950285 1107 66-67 0.9611968071557477 1107 68-69 0.929680524197142 1107 70-71 1.1006121748661606 1107 72-73 1.38478408032951 1107 74-75 1.5396607066733061 1107 76-77 1.5840586385653381 1107 78-79 1.67088540667417 1107 80-81 1.8073496681565864 1107 82-83 1.7524408291200615 1107 84-85 1.9114115284981672 1107 86-87 1.8983355397961255 1107 88-89 2.049517286622816 1107 90-91 2.4937030763035573 1107 92-93 2.749850161189631 1107 94-95 3.0383074771941416 1107 96-97 3.222947386126844 1107 98-99 3.2496998271926074 1107 100-101 3.341702578575143 1108 1 0.28199750934042456 1108 2 0.07290227696391582 1108 3 0.14409757679086965 1108 4 0.07844582179885862 1108 5 0.11586835015357622 1108 6 0.06605063079250328 1108 7 0.11711187777748933 1108 8 0.06535677236371384 1108 9 0.1530871574588204 1108 10-11 0.17070193690290836 1108 12-13 0.13232552608858583 1108 14-15 0.30953215366572095 1108 16-17 0.33493117545047113 1108 18-19 0.3569909394015127 1108 20-21 0.21608466384285663 1108 22-23 0.15024328217755567 1108 24-25 0.25701067418662404 1108 26-27 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22672.0 15 27651.0 16 33326.0 17 40998.0 18 39468.0 19 32735.0 20 32548.0 21 33895.0 22 36707.0 23 40286.0 24 45561.0 25 50664.0 26 57032.0 27 65993.0 28 76638.0 29 89218.0 30 105546.0 31 128017.0 32 155492.0 33 198010.0 34 261287.0 35 357819.0 36 469003.0 37 505522.0 38 250791.0 39 14455.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.06490321124599 18.16277704718123 14.565249275202788 23.207070466369995 2 15.631549782171428 19.465975575033752 40.20126564064256 24.701209002152265 3 19.241076076106726 23.567701794889068 29.745984310795663 27.44523781820854 4 12.760083508431988 16.243663837529677 36.47182998966862 34.524422664369716 5 13.585791465678543 37.14000167232736 34.030973585497435 15.243233276496666 6 32.73971998569055 35.88914589264975 16.641898745963214 14.729235375696485 7 28.84950880525443 33.08190334825625 19.919947778984103 18.148640067505216 8 27.032090964198463 34.25145575782068 19.198828046069913 19.517625231910944 9 26.59164494139151 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29.719867299358864 22.88257867025948 34-35 22.477368298900732 26.10692181308355 29.392154425268384 22.02355546274733 36-37 22.71118144160746 25.44871793890483 29.35843901405516 22.481661605432546 38-39 21.98980429742726 25.942456589746104 29.23763958174569 22.830099531080943 40-41 22.580572584773876 25.775038817944797 29.37846137834506 22.26592721893627 42-43 22.16120355657482 25.797794267323486 29.184557238759893 22.856444937341802 44-45 22.100358983533468 26.179398719033138 29.429931332940427 22.290310964492967 46-47 22.779045441827687 26.604419838088656 28.357780848470306 22.25875387161335 48-49 22.389522427342904 26.66914415524425 28.22497228896374 22.71636112844911 50-51 22.680019356574483 26.944556759236843 28.1447164111858 22.230707473002873 52-53 23.12362369585402 26.361405742075956 28.232314706936073 22.28265585513395 54-55 22.323280090477652 26.88276011943295 28.46304296923423 22.33091682085516 56-57 22.467999873283492 26.96605329041296 28.14825096785854 22.417695868445005 58-59 22.465996146814067 26.381483771947345 28.103939606383697 23.048580474854894 60-61 21.871788510744388 26.839835651331917 28.54527370554457 22.74310213237913 62-63 22.18583080229231 26.796416762138662 28.650598803036697 22.36715363253233 64-65 21.841242225644585 26.89959494305527 28.42346731621882 22.83569551508132 66-67 21.652451430106105 26.84412566539473 28.498643004106565 23.0047799003926 68-69 22.52178188714784 26.39430782394154 28.63504968977463 22.448860599135994 70-71 22.290947518887553 26.74437735161802 27.91964879443348 23.045026335060946 72-73 22.188116228370554 26.93319233173737 27.970900821140443 22.90779061875163 74-75 22.22621477254502 27.362010115095636 27.916629427802924 22.49514568455642 76-77 22.497785271493044 26.846916771964146 27.945642474797083 22.709655481745724 78-79 22.623886154788863 27.157560986210406 27.84896912407148 22.36958373492925 80-81 22.890820595958107 27.190100820049594 27.427960044180416 22.491118539811882 82-83 22.647745606036814 27.316672956546572 27.56765599896913 22.46792543844748 84-85 22.69019137932222 27.475278927461645 27.51852693550522 22.31600275771092 86-87 22.99449766688712 27.468268777297055 26.991103046558507 22.546130509257317 88-89 22.838858313459774 27.568962316176894 26.84039687225018 22.751782498113155 90-91 22.730999157531766 27.54291016447723 26.919768370215806 22.806322307775197 92-93 22.968653272983968 27.03961862056036 27.21653460459813 22.77519350185754 94-95 22.795026587521466 26.80579323265688 27.387091046571786 23.012089133249862 96-97 23.171486352603047 26.417043071901396 27.29787035068555 23.113600224810007 98-99 22.930620555940394 26.764545537750152 26.81108868727503 23.493745219034416 100-101 23.709296388310143 25.746124333929753 27.079245579095705 23.465333698664395 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1253.0 1 901.5 2 788.0 3 1073.0 4 893.0 5 823.5 6 1583.0 7 3422.5 8 5740.0 9 6589.5 10 5299.0 11 3904.0 12 3653.0 13 3903.5 14 4246.5 15 4635.5 16 4883.5 17 5193.5 18 5721.0 19 6244.0 20 7053.0 21 8249.5 22 9861.0 23 11895.0 24 14342.5 25 17789.0 26 22374.5 27 27344.0 28 31581.5 29 35462.5 30 39409.5 31 46195.5 32 57817.0 33 61597.5 34 58683.5 35 60722.5 36 65977.5 37 71455.5 38 75368.0 39 78836.0 40 83652.5 41 90245.5 42 94557.5 43 97637.5 44 101463.0 45 107362.0 46 110712.0 47 114120.0 48 116774.0 49 114234.0 50 113996.0 51 113453.0 52 111858.0 53 113971.5 54 117438.0 55 112290.0 56 103621.5 57 96557.0 58 90732.0 59 81146.0 60 65337.5 61 54748.5 62 47485.5 63 35838.5 64 25750.5 65 20425.5 66 15309.5 67 11464.0 68 8945.5 69 6854.0 70 5204.5 71 3593.0 72 2449.5 73 1847.0 74 1416.0 75 983.0 76 659.5 77 443.5 78 301.0 79 189.0 80 128.0 81 82.0 82 50.5 83 37.5 84 27.0 85 22.5 86 18.5 87 12.5 88 10.0 89 9.0 90 6.5 91 3.5 92 4.0 93 4.0 94 1.5 95 1.0 96 2.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4693926203939434 2 0.03969241287331755 3 0.05162767965866361 4 0.05658540586180736 5 0.08311842202307669 6 0.08112921089218568 7 0.046180301484838995 8 0.03938638039164201 9 0.04474194882096396 10-11 0.04948545228693483 12-13 0.044680742324628854 14-15 0.038177552089023625 16-17 0.03895793491729625 18-19 0.047848178509970696 20-21 0.0557591181612834 22-23 0.047236113546619614 24-25 0.06469526662620917 26-27 0.05650889774138848 28-29 0.044558329331958635 30-31 0.04644042909426321 32-33 0.0452469024157286 34-35 0.04997510425761569 36-37 0.04469604394871263 38-39 0.026272888551845116 40-41 0.02370221570577058 42-43 0.02374812057802191 44-45 0.014857876985347471 46-47 0.02757352659896616 48-49 0.02686965189111242 50-51 0.015913689047128084 52-53 0.015255719211525673 54-55 0.01597489554346319 56-57 0.015041496474352796 58-59 0.01409279578115862 60-61 0.016785881619903374 62-63 0.016097308536133406 64-65 0.01700010435707625 66-67 0.009165672826182424 68-69 0.012409617131943152 70-71 0.018193631035610856 72-73 0.016051403663882077 74-75 0.013189999960215776 76-77 0.009073863081679763 78-79 0.013893874668069519 80-81 0.017397946583254456 82-83 0.014505939631420599 84-85 0.012991078847126677 86-87 0.0031827378094256165 88-89 0.028568132164411664 90-91 0.0316743618534184 92-93 0.01651045238639539 94-95 0.032347633313104585 96-97 0.026578921033520657 98-99 0.02344208809634637 100-101 0.14790549839378853 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3267627.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.094239587596356 #Duplication Level Percentage of deduplicated Percentage of total 1 71.51034308712538 32.962148872491724 2 14.649317916663843 13.504983396911422 3 5.5146664905983895 7.6258307538999395 4 2.6940182716845404 4.967148946735579 5 1.5523267068171598 3.577665957112731 6 0.9303288721328187 2.5729681156409057 7 0.6100462878414216 1.9683733825900378 8 0.45150374595455434 1.6649377472581173 9 0.3309917098085835 1.3731130056082512 >10 1.5773838350633846 12.892592497955139 >50 0.10713108548116483 3.4008544062495982 >100 0.06147729029264684 5.49651482806301 >500 0.005465639771123949 1.6898660816139306 >1k 0.004532481760207101 4.347621533054247 >5k 3.3327071766228684E-4 0.9937587432893047 >10k+ 1.3330828706491473E-4 0.9616217315261075 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17683 0.5411572373468575 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13616 0.4166938270494153 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8837 0.27044090405667476 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6219 0.19032160035401838 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6191 0.18946470940532686 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5673 0.1736122268545339 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5425 0.1660226213089805 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4877 0.1492520413131609 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4538 0.13887754018436008 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4352 0.13318533602519506 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4107 0.1256875402241443 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3893 0.11913844511628774 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3448 0.1055199996817262 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3391 0.10377561453617565 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3389 0.10371440803984054 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.060324816755401E-5 2 0.0 0.0 0.0 0.0 9.180974450266202E-5 3 0.0 0.0 0.0 0.0 9.180974450266202E-5 4 0.0 0.0 0.0 0.0 9.180974450266202E-5 5 0.0 0.0 0.0 0.0 9.180974450266202E-5 6 0.0 0.0 0.0 0.0 9.180974450266202E-5 7 0.0 0.0 0.0 0.0 9.180974450266202E-5 8 0.0 0.0 0.0 0.0 9.180974450266202E-5 9 0.0 0.0 0.0 0.0 9.180974450266202E-5 10-11 0.0 0.0 0.0 0.0 9.180974450266202E-5 12-13 0.0 0.0 0.0 0.0 1.6831786492154706E-4 14-15 0.0 0.0 0.0 1.5301624083777005E-5 2.2952436125665506E-4 16-17 0.0 0.0 0.0 4.590487225133102E-5 3.3663572984309406E-4 18-19 0.0 0.0 0.0 7.650812041888502E-5 3.3663572984309406E-4 20-21 0.0 0.0 0.0 1.2241299267021604E-4 4.284454743457561E-4 22-23 0.0 0.0 0.0 2.7542923350798605E-4 4.590487225133101E-4 24-25 0.0 0.0 0.0 5.661600910997491E-4 5.049535947646412E-4 26-27 0.0 0.0 0.0 0.0012241299267021603 5.202552188484182E-4 28-29 0.0 0.0 0.0 0.004575185601049324 5.202552188484182E-4 30-31 0.0 0.0 0.0 0.014383526638750383 5.202552188484182E-4 32-33 0.0 0.0 0.0 0.03476528991834135 5.508584670159721E-4 34-35 0.0 0.0 0.0 0.06284377011207215 6.120649633510802E-4 36-37 0.0 0.0 0.0 0.10994216904193778 6.426682115186342E-4 38-39 0.0 0.0 0.0 0.18129364214458993 6.426682115186342E-4 40-41 0.0 0.0 0.0 0.2556289319435786 6.426682115186342E-4 42-43 0.0 0.0 0.0 0.32847996420644093 6.885730837699652E-4 44-45 0.0 0.0 0.0 0.4216668548766429 7.803828282726272E-4 46-47 0.0 0.0 0.0 0.5283956828609875 8.262877005239582E-4 48-49 0.0 0.0 0.0 0.6376186755709878 8.721925727752892E-4 50-51 0.0 0.0 0.0 0.76001636661712 8.874941968590662E-4 52-53 0.0 0.0 0.0 0.8714427931951841 9.027958209428433E-4 54-55 0.0 0.0 0.0 0.9893265051366023 9.180974450266203E-4 56-57 0.0 0.0 0.0 1.1260159130769822 9.640023172779514E-4 58-59 0.0 0.0 0.0 1.2689024787712917 9.793039413617283E-4 60-61 0.0 0.0 0.0 1.424596503823723 9.946055654455053E-4 62-63 0.0 0.0 0.0 1.5765569325997122 0.0010405104376968364 64-65 0.0 0.0 0.0 1.7303076513935036 0.0010405104376968364 66-67 0.0 0.0 0.0 1.9082502378637463 0.0010711136858643903 68-69 0.0 0.0 0.0 2.1030552140743115 0.0011017169340319442 70-71 0.0 0.0 0.0 2.3009358167257155 0.0011782250544508292 72-73 0.0 0.0 0.0 2.5082116165645587 0.0013159396712048223 74-75 0.0 0.0 0.0 2.711860931495547 0.0013771461675399304 76-77 0.0 0.0 0.0 2.931255617608742 0.0014077494157074843 78-79 3.060324816755401E-5 0.0 0.0 3.1802130414517937 0.0014077494157074843 80-81 3.060324816755401E-5 0.0 0.0 3.430302785477045 0.0014536542879588152 82-83 3.060324816755401E-5 0.0 0.0 3.6923124946635584 0.0015607656565452543 84-85 3.060324816755401E-5 0.0 0.0 3.963931623774684 0.0015607656565452543 86-87 6.120649633510802E-5 0.0 0.0 4.23591799186382 0.0015760672806290313 88-89 6.120649633510802E-5 0.0 0.0 4.521966552485948 0.0015913689047128085 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7400 0.0 58.564457 1 GTATCAA 13545 0.0 42.601734 1 TATCAAC 16995 0.0 33.89336 2 ATCAACG 16955 0.0 33.862236 3 TCAACGC 17290 0.0 33.234142 4 CAACGCA 17400 0.0 32.96941 5 AACGCAG 18005 0.0 31.935879 6 ACGCAGA 20365 0.0 27.994778 7 CGCAGAG 20625 0.0 27.61884 8 TGGTATC 3075 0.0 26.429209 2 GTGGTAT 3465 0.0 25.069641 1 GCAGAGT 22280 0.0 24.990368 9 GTGTAAG 1520 0.0 24.806385 1 TAAGGTA 1310 0.0 24.671227 4 AGGTAAG 2190 0.0 24.522657 2 TAAGGTG 2455 0.0 24.199793 5 GAGTACT 15140 0.0 23.988066 12-13 GTACATG 11420 0.0 23.32105 1 CAGAGTA 21060 0.0 22.883408 10-11 GTACTTT 15875 0.0 22.698065 14-15 >>END_MODULE