FastQCFastQC Report
Thu 26 May 2016
SRR937982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937982_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3267627
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174860.5351283974578493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141520.43309716806722426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94610.2895373309132285No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA64710.198033618892242No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA63500.19433062586396793No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA57200.1750505795184089No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA56590.17318378138018814No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC53430.16351315495924107No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG47400.145059396314206No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA45050.13786763299483082No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA41270.1262996051874954No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA39400.12057679778016278No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA35900.10986566092151889No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34910.10683593935293104No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG33480.10245967486497082No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT32730.10016443125240428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72600.056.0802881
GTATCAA135300.043.2241331
ATCAACG172800.033.6732673
TATCAAC172550.033.672672
TCAACGC175750.033.2141274
CAACGCA177600.032.868155
AACGCAG183200.031.914816
ACGCAGA207750.028.0519587
TGGTATC30250.027.7980232
CGCAGAG209000.027.7932838
AGGTAAG22650.027.2672392
TAAGGTG26050.026.8025385
GTGGTAT34300.025.346791
GCAGAGT227400.025.2310989
GTAAGGT26450.025.1401984
GGACCGA12950.024.2066026
GGTAAGG27350.023.9670563
AAGGTAA24200.023.5576481
GAGTACT158950.022.94881812-13
ACCGAGT13900.022.5521958
Produced by FastQC (version 0.11.5)