FastQCFastQC Report
Thu 26 May 2016
SRR937981_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937981_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3279625
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180260.5496360102145824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141470.43136029271639287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92570.2822578800929984No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA64680.19721766970309104No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA63060.19227808057323628No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA57220.17447116667301904No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA56140.17117810725311583No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC49600.1512368029881465No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA45450.13858291725425925No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG44750.1364485268895072No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA42080.1283073522125243No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA39200.1195258604261158No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT34840.10623165758280291No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34670.10571330563707741No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA34010.10370088043602547No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG33830.10315203719937494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69850.056.0120431
GTATCAA134900.046.2128221
TATCAAC175450.035.2095642
ATCAACG176450.034.929233
TCAACGC179600.034.395984
CAACGCA181600.033.9910055
AACGCAG185600.033.3864566
TGGTATC28800.032.1745762
GTGGTAT31800.030.1948471
ACGCAGA209950.029.324027
CGCAGAG211400.029.0779088
GCAGAGT229650.026.2073789
AGGTAAG21650.025.6802142
TAAGGTG24900.025.3818325
GTAAGGT24650.023.904274
GTACATG113100.023.8643591
TACATGG110200.023.4147192
GAGTACT157200.023.2536112-13
AAGGTAA23700.022.9784341
GGTAAGG24950.022.8550073
Produced by FastQC (version 0.11.5)