##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937981_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3279625 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.90514677745169 33.0 31.0 34.0 30.0 34.0 2 32.091214696802226 34.0 31.0 34.0 30.0 34.0 3 32.1558501353051 34.0 31.0 34.0 30.0 34.0 4 35.679502991957925 37.0 35.0 37.0 35.0 37.0 5 35.70170370088044 37.0 35.0 37.0 35.0 37.0 6 35.7338442657316 37.0 36.0 37.0 35.0 37.0 7 35.67484819148531 37.0 35.0 37.0 33.0 37.0 8 35.65258710980676 37.0 35.0 37.0 33.0 37.0 9 37.31904836681023 39.0 37.0 39.0 34.0 39.0 10-11 37.24252986240805 39.0 37.0 39.0 34.0 39.0 12-13 37.14263017875519 39.0 37.0 39.0 34.0 39.0 14-15 38.395907154019135 40.0 38.0 41.0 34.0 41.0 16-17 38.3254222662652 40.0 38.0 41.0 33.5 41.0 18-19 38.2826419179022 40.0 38.0 41.0 33.5 41.0 20-21 38.17655356938674 40.0 38.0 41.0 33.5 41.0 22-23 38.037761329420285 40.0 38.0 41.0 33.0 41.0 24-25 37.9785844418188 40.0 38.0 41.0 33.0 41.0 26-27 37.968182490376186 40.0 38.0 41.0 33.0 41.0 28-29 37.87263955482715 40.0 38.0 41.0 33.0 41.0 30-31 37.66240743987498 40.0 37.5 41.0 32.5 41.0 32-33 37.41315196097115 40.0 37.0 41.0 32.0 41.0 34-35 37.19281305027252 40.0 36.5 41.0 31.0 41.0 36-37 36.94950596485879 40.0 36.0 41.0 31.0 41.0 38-39 36.690408507070174 39.5 36.0 41.0 30.0 41.0 40-41 36.442664633913935 39.0 35.5 41.0 30.0 41.0 42-43 36.13129778557 39.0 35.0 41.0 29.5 41.0 44-45 35.89097091893128 39.0 35.0 41.0 28.5 41.0 46-47 35.71286366581545 39.0 35.0 41.0 27.5 41.0 48-49 35.420270457750505 39.0 35.0 41.0 26.0 41.0 50-51 34.73663315165606 38.5 34.5 40.0 25.0 40.5 52-53 34.78853618935092 38.5 34.5 40.0 25.5 41.0 54-55 34.90783412737737 39.0 35.0 40.0 25.0 41.0 56-57 34.68126828524603 38.0 34.5 40.0 24.0 41.0 58-59 34.396746579258306 38.0 34.0 40.0 23.5 41.0 60-61 34.015336357053016 37.5 34.0 40.0 22.5 41.0 62-63 33.59194603041506 37.0 33.5 40.0 21.5 41.0 64-65 33.16050615542936 36.0 33.0 39.5 19.5 41.0 66-67 32.73061020695964 36.0 33.0 39.0 18.0 41.0 68-69 32.253802645119485 35.0 32.0 39.0 15.0 40.0 70-71 31.766333955863857 35.0 31.5 38.0 9.5 40.0 72-73 31.30011342760224 35.0 31.0 37.0 7.5 39.5 74-75 30.79618599687464 35.0 31.0 37.0 4.5 39.0 76-77 30.276017380035828 35.0 30.0 36.0 2.0 39.0 78-79 29.723728627510766 34.0 29.5 36.0 2.0 37.5 80-81 29.219472653123454 34.0 29.0 35.0 2.0 37.0 82-83 28.69813698212448 34.0 29.0 35.0 2.0 37.0 84-85 28.231381789076494 34.0 27.5 35.0 2.0 36.0 86-87 27.757194038952626 34.0 26.5 35.0 2.0 36.0 88-89 27.207573884209324 33.0 25.5 35.0 2.0 35.0 90-91 26.616132942028432 33.0 25.0 35.0 2.0 35.0 92-93 26.021073293440562 32.5 23.5 35.0 2.0 35.0 94-95 25.436427792811678 32.0 21.0 35.0 2.0 35.0 96-97 24.625711781072532 32.0 12.5 34.0 2.0 35.0 98-99 23.57933117353356 31.0 2.0 34.0 2.0 35.0 100-101 20.762966192781185 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.41168301235134663 1101 2 0.3655639807617206 1101 3 0.3547081167657993 1101 4 0.32356640152600846 1101 5 0.3144157045873328 1101 6 0.32421012110646075 1101 7 0.3224120046145984 1101 8 0.34790974206658376 1101 9 0.4307762540834048 1101 10-11 0.4145878806726131 1101 12-13 0.3657459812218633 1101 14-15 0.4800675869297564 1101 16-17 0.6234494538026709 1101 18-19 0.5127757258743131 1101 20-21 0.5567879582228201 1101 22-23 0.5658967923697062 1101 24-25 0.5896677423711623 1101 26-27 0.5600744675426554 1101 28-29 0.5980964217287763 1101 30-31 0.6359928047513677 1101 32-33 0.6787522310733394 1101 34-35 0.6450119798923168 1101 36-37 0.6672475115111851 1101 38-39 0.7058307543161391 1101 40-41 0.761563322549307 1101 42-43 0.6821415334706771 1101 44-45 0.7960857835058235 1101 46-47 0.9221526685618002 1101 48-49 0.9621172215369782 1101 50-51 1.0177711094628847 1101 52-53 0.9810656448998216 1101 54-55 0.9133462677177064 1101 56-57 0.8595058141486831 1101 58-59 0.8631489167091573 1101 60-61 0.8974007132998807 1101 62-63 0.9683274211790973 1101 64-65 0.9896273504624205 1101 66-67 0.8383878634449715 1101 68-69 0.9352958700861151 1101 70-71 1.085621907720327 1101 72-73 1.0417333862718294 1101 74-75 0.8701933117236109 1101 76-77 0.991030705847475 1101 78-79 0.925660977265629 1101 80-81 0.9896928318327234 1101 82-83 1.004680497360912 1101 84-85 1.012410595923189 1101 86-87 1.094807669209164 1101 88-89 1.0264011232276502 1101 90-91 0.9647142829538922 1101 92-93 1.150991542995353 1101 94-95 1.3534633211102616 1101 96-97 1.378055436521013 1101 98-99 1.423612379807814 1101 100-101 1.5604042601355026 1102 1 0.28326330108635744 1102 2 0.2037123346973928 1102 3 0.15596488823227617 1102 4 0.035682061754535255 1102 5 -0.01343840696180365 1102 6 0.15631857505975688 1102 7 0.01393270224462384 1102 8 0.09938014732667 1102 9 0.1596922156652525 1102 10-11 0.2305222434342582 1102 12-13 0.09319783203436316 1102 14-15 0.1524445381913324 1102 16-17 0.19752491533550653 1102 18-19 0.14840641795785103 1102 20-21 0.24089625133048997 1102 22-23 0.2422726397120485 1102 24-25 0.16420166283177196 1102 26-27 0.19922487996013416 1102 28-29 0.18919120844821435 1102 30-31 0.281012921454888 1102 32-33 0.3258364518483887 1102 34-35 0.29080699118451037 1102 36-37 0.26818523174196685 1102 38-39 0.1593323122942678 1102 40-41 0.13286940371182254 1102 42-43 0.314790653623092 1102 44-45 0.029270509820143786 1102 46-47 0.15133171541211965 1102 48-49 0.26928280868829546 1102 50-51 0.2759558418883614 1102 52-53 0.36968353117804753 1102 54-55 0.41212073648102177 1102 56-57 0.32079622386258677 1102 58-59 0.3450322723400703 1102 60-61 0.22095287653912976 1102 62-63 0.3618452323878856 1102 64-65 0.3176621688963124 1102 66-67 0.27945676330732283 1102 68-69 0.23374548201547896 1102 70-71 0.3757841809139286 1102 72-73 0.40136282043500415 1102 74-75 0.3287792293222509 1102 76-77 0.21176083499575427 1102 78-79 0.08305319052835358 1102 80-81 0.217865737948987 1102 82-83 0.2380491083611389 1102 84-85 0.3942155820887514 1102 86-87 0.21546670116782352 1102 88-89 0.3824202086124764 1102 90-91 0.43785672984611423 1102 92-93 0.5091266161666717 1102 94-95 0.6844775114590824 1102 96-97 0.787806067131207 1102 98-99 0.9388746865150068 1102 100-101 0.8426043692074181 1103 1 0.34850858937052465 1103 2 0.3461491890049828 1103 3 0.2442981606132335 1103 4 0.18134582033280822 1103 5 0.18832242569271074 1103 6 0.13564884741904848 1103 7 0.16150702668069528 1103 8 0.19076992908377122 1103 9 0.2783562451516062 1103 10-11 0.27661761472624846 1103 12-13 0.1532184727610968 1103 14-15 0.30962701493411515 1103 16-17 0.22166112503094126 1103 18-19 0.27093583757397255 1103 20-21 0.25116367930399264 1103 22-23 0.3168077691294755 1103 24-25 0.45935357285022604 1103 26-27 0.34213733751573017 1103 28-29 0.3919684122901188 1103 30-31 0.38724726649675034 1103 32-33 0.47571902728743254 1103 34-35 0.4712572227142502 1103 36-37 0.48145179621052137 1103 38-39 0.37662074368056864 1103 40-41 0.44410441379458376 1103 42-43 0.39991647214170456 1103 44-45 0.46443222424203157 1103 46-47 0.42157271878298275 1103 48-49 0.3755346823915531 1103 50-51 0.38240361730905903 1103 52-53 0.4530751186784485 1103 54-55 0.4105589968085397 1103 56-57 0.4360900333450175 1103 58-59 0.5358983722350317 1103 60-61 0.4707617500716381 1103 62-63 0.5346349730079609 1103 64-65 0.5052159923690667 1103 66-67 0.5694896636147817 1103 68-69 0.6210685677400676 1103 70-71 0.7262037273863164 1103 72-73 0.7957843734441212 1103 74-75 0.6800913981266383 1103 76-77 0.6690496600490015 1103 78-79 0.703958326344619 1103 80-81 0.7045544957760264 1103 82-83 0.721559646173084 1103 84-85 0.752884256379204 1103 86-87 0.7283008842238843 1103 88-89 0.6630241449558341 1103 90-91 0.6263840637262312 1103 92-93 0.629541706515564 1103 94-95 0.7301449737677999 1103 96-97 0.652944156877485 1103 98-99 0.7376054716662637 1103 100-101 0.7568276025920504 1104 1 0.24800651399528562 1104 2 0.2096628114767256 1104 3 0.08781928356776092 1104 4 0.06031716009610477 1104 5 0.05911090131295538 1104 6 0.1373889638193333 1104 7 0.1376739899760011 1104 8 0.12538028515458421 1104 9 0.06881134254693677 1104 10-11 0.2166951504727237 1104 12-13 0.15298312149617743 1104 14-15 0.17888079159416748 1104 16-17 0.28134137246338753 1104 18-19 0.33716988998551756 1104 20-21 0.26346098886281055 1104 22-23 0.3108237503866178 1104 24-25 0.40156316586664786 1104 26-27 0.34755656814723324 1104 28-29 0.4572796790302718 1104 30-31 0.42847515089264476 1104 32-33 0.39264519366853534 1104 34-35 0.39822874131156283 1104 36-37 0.5202301600199704 1104 38-39 0.33506951905209803 1104 40-41 0.33630809694027164 1104 42-43 0.38170616115287714 1104 44-45 0.39527569681177255 1104 46-47 0.3597255370553327 1104 48-49 0.34245698209142716 1104 50-51 0.37286906247062035 1104 52-53 0.3749148812114953 1104 54-55 0.3135290254153986 1104 56-57 0.21095224437925708 1104 58-59 0.26638854877759144 1104 60-61 0.22009145209332104 1104 62-63 0.0969819122990856 1104 64-65 0.16299707318116674 1104 66-67 0.2487856398276591 1104 68-69 0.22910572830422637 1104 70-71 0.29633153877663787 1104 72-73 0.2786968280463178 1104 74-75 0.21871871970071766 1104 76-77 0.33180458913384925 1104 78-79 0.42988224895211147 1104 80-81 0.41757979595461947 1104 82-83 0.4699735379284178 1104 84-85 0.4175286414417769 1104 86-87 0.37565543714216076 1104 88-89 0.34992501533259457 1104 90-91 0.4677033753603048 1104 92-93 0.5093647597129696 1104 94-95 0.518561165759067 1104 96-97 0.42463761166269265 1104 98-99 0.5028572771976805 1104 100-101 0.4281319066696234 1105 1 0.38512831933793734 1105 2 0.3254680033446462 1105 3 0.27255991634237375 1105 4 0.18577952784922758 1105 5 0.15014188666213357 1105 6 0.22234420720385373 1105 7 0.21424263270210986 1105 8 0.2094594950658717 1105 9 0.26713758083005246 1105 10-11 0.296199736706491 1105 12-13 0.14803250204049334 1105 14-15 0.2492800075366688 1105 16-17 0.2571855159057037 1105 18-19 0.31233959319964555 1105 20-21 0.35610341340983354 1105 22-23 0.2617979920874234 1105 24-25 0.34681805994349446 1105 26-27 0.2501704449597355 1105 28-29 0.3554584541917265 1105 30-31 0.32399280475136294 1105 32-33 0.3546040306499094 1105 34-35 0.3883161436185034 1105 36-37 0.4547563329649549 1105 38-39 0.4356783195948992 1105 40-41 0.3629719322881968 1105 42-43 0.3840836647339074 1105 44-45 0.5364979218262249 1105 46-47 0.41631639474386617 1105 48-49 0.4066479201961144 1105 50-51 0.3819150755885019 1105 52-53 0.37060975216869707 1105 54-55 0.4104443622836911 1105 56-57 0.47615366171396545 1105 58-59 0.4385222406329419 1105 60-61 0.4517987372942329 1105 62-63 0.366636524919393 1105 64-65 0.366357061118741 1105 66-67 0.5715734668324117 1105 68-69 0.4886607254142703 1105 70-71 0.5729924087789051 1105 72-73 0.5608978181702113 1105 74-75 0.5964459581597445 1105 76-77 0.5818267538361788 1105 78-79 0.6168324663270255 1105 80-81 0.6171113215998325 1105 82-83 0.7554836025126406 1105 84-85 0.7383626072146505 1105 86-87 0.7495190312416291 1105 88-89 0.7721809397414106 1105 90-91 0.7439873951627867 1105 92-93 0.7666104561922502 1105 94-95 0.9317413733332671 1105 96-97 0.9269827477418993 1105 98-99 1.030502993780999 1105 100-101 1.0455686920338785 1106 1 0.03429997851387867 1106 2 0.05159351808686097 1106 3 -0.058854912939693804 1106 4 0.06011898710517727 1106 5 0.07781951096502837 1106 6 0.14911324972450046 1106 7 0.10376462248962781 1106 8 0.07928192959066394 1106 9 0.11710619037896208 1106 10-11 -0.4985752827066463 1106 12-13 -0.25938449210026704 1106 14-15 -0.1275226844180537 1106 16-17 0.08081508975796936 1106 18-19 0.1218340986941584 1106 20-21 -0.012265647935670643 1106 22-23 -0.05277531896982879 1106 24-25 -0.0489583948650818 1106 26-27 -0.09421165260008735 1106 28-29 -0.11621276965936289 1106 30-31 0.03268551247190743 1106 32-33 -0.01236568485942513 1106 34-35 -0.24754972926664465 1106 36-37 -0.3166369418544477 1106 38-39 -0.1538045615496486 1106 40-41 0.09120013663203252 1106 42-43 0.0013594253758313357 1106 44-45 0.13373951535047013 1106 46-47 0.3721913976852633 1106 48-49 0.2718701711785414 1106 50-51 0.36013537349187885 1106 52-53 0.24248555516656012 1106 54-55 -0.05231790328799946 1106 56-57 0.06157992709740512 1106 58-59 0.28064606113792223 1106 60-61 0.030898156076737848 1106 62-63 0.03641053249639725 1106 64-65 0.21083276528609218 1106 66-67 0.02702481633943421 1106 68-69 -0.22092319815680383 1106 70-71 0.02320503555255371 1106 72-73 0.21227095039130717 1106 74-75 0.3125294464059749 1106 76-77 0.3704747054562958 1106 78-79 0.4987184818104744 1106 80-81 0.42172618853161836 1106 82-83 0.5104367050845866 1106 84-85 0.42675392895500863 1106 86-87 0.42956783468657633 1106 88-89 0.23791805059629567 1106 90-91 0.058387705772947385 1106 92-93 0.374105997053956 1106 94-95 0.3655668198353723 1106 96-97 0.3645482229519139 1106 98-99 0.24473990462176687 1106 100-101 -0.03557110606951497 1107 1 0.3096372400749914 1107 2 0.22813311010100534 1107 3 0.15946485930815157 1107 4 0.26437209877639845 1107 5 0.282031912985623 1107 6 0.22392595625328227 1107 7 0.11199987166087055 1107 8 0.14358877263241254 1107 9 0.20429230443344437 1107 10-11 0.21402424679715892 1107 12-13 0.14657008612940814 1107 14-15 0.17233704175720277 1107 16-17 0.1814973029779523 1107 18-19 0.32020037120491196 1107 20-21 0.3132078299058847 1107 22-23 0.4070468952754922 1107 24-25 0.3908590075890004 1107 26-27 0.41569779213727287 1107 28-29 0.41672666126982705 1107 30-31 0.40251810454662973 1107 32-33 0.41821210318574487 1107 34-35 0.4881745817583081 1107 36-37 0.40304530634898583 1107 38-39 0.44975904495319696 1107 40-41 0.4634042224315138 1107 42-43 0.5114413716657609 1107 44-45 0.44388370714356284 1107 46-47 0.4356612324157041 1107 48-49 0.3999061828460171 1107 50-51 0.35560796824717045 1107 52-53 0.44412890981713105 1107 54-55 0.6197991443837125 1107 56-57 0.54626773015503 1107 58-59 0.359806826769244 1107 60-61 0.40519914677069124 1107 62-63 0.48346074252688 1107 64-65 0.3828730014521753 1107 66-67 0.43353076428780923 1107 68-69 0.5456727172247398 1107 70-71 0.5994685713995445 1107 72-73 0.6271183504895994 1107 74-75 0.6159838347318427 1107 76-77 0.6710762419906118 1107 78-79 0.5787423815069062 1107 80-81 0.7890580896606707 1107 82-83 0.6692110081868137 1107 84-85 0.6308112764161713 1107 86-87 0.6980180075504911 1107 88-89 0.7436960026252954 1107 90-91 0.9364091559115444 1107 92-93 0.8517014184619178 1107 94-95 0.8437214844768732 1107 96-97 0.9054676848580137 1107 98-99 0.9636711716107769 1107 100-101 0.7655610874711449 1108 1 0.1531364165039264 1108 2 0.036513437442707186 1108 3 0.09299446425060154 1108 4 0.0012253218213373884 1108 5 0.04197454523163913 1108 6 0.07101087387052019 1108 7 0.05591784637732644 1108 8 0.06762233807081941 1108 9 0.12167829158128995 1108 10-11 0.10189654282434901 1108 12-13 -0.044601326119646956 1108 14-15 -0.014532857960404044 1108 16-17 0.08353459372846572 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66-67 -0.9469750964719914 2316 68-69 -0.8982354882942012 2316 70-71 -0.8738287525500432 2316 72-73 -0.9497447289713641 2316 74-75 -1.0106103124179278 2316 76-77 -0.9553466972263749 2316 78-79 -0.9122198383721631 2316 80-81 -0.9407612464935511 2316 82-83 -0.8192560712377315 2316 84-85 -0.8543216938928033 2316 86-87 -1.122107502730234 2316 88-89 -1.0936500240418852 2316 90-91 -1.1134680822391907 2316 92-93 -1.0653855031013784 2316 94-95 -1.1601951726110613 2316 96-97 -1.2449706344789924 2316 98-99 -1.3551272815856557 2316 100-101 -1.3120694402618405 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9242.0 3 2507.0 4 2451.0 5 2747.0 6 3202.0 7 3966.0 8 4887.0 9 6264.0 10 7979.0 11 9966.0 12 12849.0 13 16451.0 14 19146.0 15 23312.0 16 28105.0 17 37589.0 18 33902.0 19 26692.0 20 24947.0 21 25424.0 22 26905.0 23 29247.0 24 32031.0 25 36306.0 26 40926.0 27 47360.0 28 55567.0 29 65814.0 30 78268.0 31 95595.0 32 120254.0 33 155997.0 34 215007.0 35 323372.0 36 487697.0 37 649336.0 38 463440.0 39 54873.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.06609291830506 18.245577905447668 14.53040250338405 23.157926672863223 2 15.644669053493724 19.445746054291774 40.23615676914404 24.673428123070465 3 19.317575804910433 23.5849465304922 29.641864739169115 27.45561292542825 4 12.77892484128672 16.23670346253842 36.460987517433516 34.523384178741345 5 13.592085468155421 37.13476614352718 34.05204744568982 15.221100942627578 6 32.787159960526566 35.868786527506415 16.627468959536674 14.716584552430353 7 28.906272407886235 33.10078460784559 19.847002385495895 18.145940598772285 8 27.05880414826819 34.259607834044864 19.14891799561792 19.53267002206902 9 26.592107531113342 16.544928438178307 19.90963823260681 36.95332579810155 10-11 25.245846163329063 26.246593458769524 26.60043739211285 21.907122985788572 12-13 26.44875407477703 23.662864940457396 27.184858091153824 22.703522893611748 14-15 22.886624609566393 24.96445749940661 25.775787533338256 26.373130357688744 16-17 22.137180996245455 28.291833784574568 26.816207828470972 22.75477739070901 18-19 22.29184174729719 26.20294408520234 29.5704568187667 21.93475734873377 20-21 22.40895901261797 25.36894777128526 30.30846686865873 21.913626347438043 22-23 22.381822519899146 25.340393735766714 30.167675150296198 22.110108594037943 24-25 22.150289585364053 25.599373648433787 30.131465468570422 22.118871297631742 26-27 21.48577905687996 26.070958642673585 29.88047954635775 22.562782754088705 28-29 21.8117244846043 26.480999372226794 29.738112546081837 21.96916359708707 30-31 21.833765670275334 25.88643820826227 29.640194871242514 22.639601250219883 32-33 21.423124945077475 25.966377041897342 29.729332654735053 22.88116535829013 34-35 22.49377875765555 26.12580037802696 29.377125142973004 22.003295721344486 36-37 22.703380720971904 25.450496836399907 29.353897573012084 22.492224869616106 38-39 21.972254429063454 25.871864866093258 29.279862147602 22.87601855724129 40-41 22.58333204958688 25.740661964748274 29.404281144438865 22.271724841225986 42-43 22.149903507542394 25.820399419138738 29.202813525751885 22.826883547566975 44-45 22.119140141020434 26.19845182198286 29.4169987280743 22.265409308922404 46-47 22.760227525567196 26.630355133927242 28.371948395451867 22.237468945053696 48-49 22.38028391323543 26.651685873040442 28.245790750287657 22.722239463436466 50-51 22.677754833739254 26.926538021115054 28.20820004449866 22.187507100647032 52-53 23.122703689148764 26.376947780177563 28.21404221010689 22.286306320566787 54-55 22.34094628580352 26.89218731986539 28.46774946184547 22.299116932485624 56-57 22.450057018002685 26.999419602786272 28.160763013417455 22.38976036579359 58-59 22.468751867995397 26.396574355671206 28.10749040231589 23.02718337401751 60-61 21.90149883398268 26.85481544108273 28.53788504039123 22.705800684543362 62-63 22.163139655491587 26.801355806904258 28.695981284155547 22.339523253448608 64-65 21.82698001189737 26.91881713715571 28.459223291643738 22.794979559303183 66-67 21.651777103165777 26.872438406245152 28.5282196733755 22.947564817213575 68-69 22.474845704308844 26.44918249015519 28.64969255225468 22.426279253281283 70-71 22.2543190824799 26.766864794450367 27.940821862984812 23.037994260084925 72-73 22.142842218299908 26.969718552699668 27.98806793632308 22.899371292677344 74-75 22.20164925360843 27.38457329314513 27.963392011282956 22.450385441963487 76-77 22.475081415045025 26.89133294986883 27.958573024064936 22.67501261102121 78-79 22.5813150909934 27.217144034290996 27.863004963387183 22.33853591132842 80-81 22.7988855838768 27.28980689848039 27.479100885582962 22.432206632059852 82-83 22.627069717822103 27.402589718651654 27.60261173830526 22.367728825220983 84-85 22.57705239103607 27.609156060874295 27.53492580710534 22.278865740984294 86-87 22.953927812127592 27.571392588490585 27.063499517584678 22.41118008179715 88-89 22.71917256433494 27.69267538121444 26.931710690354343 22.656441364096278 90-91 22.650904798818516 27.69869285651746 27.05540205331993 22.595000291344093 92-93 22.84256140482515 27.24046363475815 27.321902597170865 22.595072363245833 94-95 22.692472609164078 26.987083554576735 27.477828245764673 22.842615590494514 96-97 23.040459569486107 26.534665304739786 27.482441957068797 22.94243316870531 98-99 22.761054610328628 26.922384771904635 27.004296757163832 23.312263860602915 100-101 23.15541663586991 26.328480578494474 27.396955060757676 23.11914772487794 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1367.0 1 964.5 2 812.0 3 1077.0 4 877.0 5 851.0 6 1675.0 7 3636.0 8 6063.0 9 6984.0 10 5638.5 11 4164.5 12 3883.5 13 4063.5 14 4362.0 15 4624.0 16 4854.0 17 5175.5 18 5681.0 19 6307.5 20 7180.0 21 8432.5 22 9890.5 23 11692.5 24 14321.5 25 17951.0 26 22478.5 27 27373.5 28 31773.5 29 35782.0 30 39537.0 31 46226.0 32 58331.0 33 61907.5 34 58215.0 35 60304.0 36 65882.0 37 71701.5 38 76068.0 39 79684.0 40 84357.5 41 90866.0 42 94846.5 43 98311.0 44 102418.0 45 108941.0 46 112146.0 47 115164.5 48 118204.5 49 115019.0 50 114192.5 51 113525.0 52 112238.5 53 114155.0 54 118180.5 55 113289.5 56 104010.0 57 97166.5 58 91731.0 59 81718.0 60 64653.5 61 53854.5 62 47364.0 63 35173.5 64 24555.5 65 19611.0 66 14650.0 67 11046.5 68 8426.0 69 6319.0 70 4910.5 71 3346.0 72 2318.5 73 1755.5 74 1297.5 75 913.5 76 622.0 77 412.0 78 264.5 79 178.0 80 119.5 81 69.0 82 39.0 83 31.0 84 27.0 85 20.0 86 14.5 87 8.5 88 4.5 89 7.5 90 6.5 91 3.0 92 3.0 93 3.5 94 2.5 95 1.0 96 1.5 97 1.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.503777108663338 2 0.04979227808057324 3 0.05698822273888021 4 0.06628806647101422 5 0.07973472576895224 6 0.10665853565575331 7 0.0707093036551435 8 0.05265845942752602 9 0.05153028166329992 10-11 0.07543545374852308 12-13 0.10749704615619164 14-15 0.056683309829629915 16-17 0.060952090559134045 18-19 0.06002210618592065 20-21 0.07420055646605937 22-23 0.06394023706978694 24-25 0.08754049624575981 26-27 0.06563250371612608 28-29 0.06608987308000153 30-31 0.06956588024545489 32-33 0.06955063459999238 34-35 0.07151732286465678 36-37 0.06671494454396464 38-39 0.04073636467583946 40-41 0.04450203910508061 42-43 0.04236764874032854 44-45 0.022517818348134315 46-47 0.02928688493349087 48-49 0.02995769333384152 50-51 0.026664633913938328 52-53 0.026237755840987916 54-55 0.025216297594999427 56-57 0.025490719213324693 58-59 0.021618325265845943 60-61 0.02875328734230285 62-63 0.02109997332012044 64-65 0.02285322254830964 66-67 0.016419560163128406 68-69 0.018675915691580593 70-71 0.041269962267027484 72-73 0.03755002477417388 74-75 0.032046346762206045 76-77 0.022776994320997068 78-79 0.015657277890002666 80-81 0.018249037618630178 82-83 0.02244159012082174 84-85 0.01946868925563136 86-87 0.008933948241033655 88-89 0.041224225330639935 90-91 0.05226207264550063 92-93 0.03341845485383237 94-95 0.05334451347333918 96-97 0.043495826504554636 98-99 0.03372336776308267 100-101 0.1668178526508366 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3279625.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.98507817765504 #Duplication Level Percentage of deduplicated Percentage of total 1 71.45852117748362 32.86025682806202 2 14.720065420776452 13.538067183092037 3 5.559682151780108 7.66987255177565 4 2.653613521972442 4.881065010447411 5 1.514526037379256 3.48227991154896 6 0.9472652630473665 2.6136040305726067 7 0.6145408146924616 1.9781795184894864 8 0.44661123273828224 1.6429961961991042 9 0.3238585817004618 1.3403395978200197 >10 1.5823784818929931 12.965731262251397 >50 0.10486156741597866 3.3265734319785722 >100 0.06365279881672888 5.629344612024198 >500 0.005311057480238483 1.6420457980219807 >1k 0.004647175294344231 4.428794762374236 >5k 3.3194109245315937E-4 1.0186502711528305 >10k+ 1.3277643698126376E-4 0.9821990341894691 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18026 0.5496360102145824 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14147 0.43136029271639287 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9257 0.2822578800929984 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6468 0.19721766970309104 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6306 0.19227808057323628 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5722 0.17447116667301904 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5614 0.17117810725311583 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4960 0.1512368029881465 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4545 0.13858291725425925 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4475 0.1364485268895072 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4208 0.1283073522125243 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3920 0.1195258604261158 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3484 0.10623165758280291 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3467 0.10571330563707741 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3401 0.10370088043602547 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3383 0.10315203719937494 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.049129092502954E-5 2 0.0 0.0 0.0 0.0 3.049129092502954E-5 3 0.0 0.0 0.0 0.0 3.049129092502954E-5 4 0.0 0.0 0.0 0.0 3.049129092502954E-5 5 0.0 0.0 0.0 0.0 3.049129092502954E-5 6 0.0 0.0 0.0 0.0 3.049129092502954E-5 7 0.0 0.0 0.0 0.0 3.049129092502954E-5 8 0.0 0.0 0.0 0.0 3.049129092502954E-5 9 0.0 0.0 0.0 0.0 3.049129092502954E-5 10-11 0.0 0.0 0.0 0.0 3.049129092502954E-5 12-13 0.0 0.0 0.0 0.0 1.3721080916263292E-4 14-15 0.0 0.0 0.0 0.0 2.2868468193772156E-4 16-17 0.0 0.0 0.0 0.0 2.8966726378778064E-4 18-19 0.0 0.0 0.0 0.0 3.354042001753249E-4 20-21 0.0 0.0 0.0 4.573693638754431E-5 3.8114113656286924E-4 22-23 0.0 0.0 0.0 1.524564546251477E-4 4.116324274878988E-4 24-25 0.0 0.0 0.0 4.421237184129283E-4 4.573693638754431E-4 26-27 0.0 0.0 0.0 0.0012806342188512406 4.8786065480047264E-4 28-29 0.0 0.0 0.0 0.004588939284216945 5.183519457255022E-4 30-31 0.0 0.0 0.0 0.014025993825513587 5.183519457255022E-4 32-33 0.0 0.0 0.0 0.03425696535427069 5.793345275755612E-4 34-35 0.0 0.0 0.0 0.061394214277546974 6.098258185005908E-4 36-37 0.0 0.0 0.0 0.10774097648359188 6.098258185005908E-4 38-39 0.0 0.0 0.0 0.17720013721080916 6.403171094256203E-4 40-41 0.0 0.0 0.0 0.2507146396310554 6.403171094256203E-4 42-43 0.0 0.0 0.0 0.3244273354423143 6.55562754888135E-4 44-45 0.0 0.0 0.0 0.41713610549986657 6.860540458131646E-4 46-47 0.0 0.0 0.0 0.5243739756831955 7.012996912756793E-4 48-49 0.0 0.0 0.0 0.6339596752677517 7.012996912756793E-4 50-51 0.0 0.0 0.0 0.7554979608949194 7.31790982200709E-4 52-53 0.0 0.0 0.0 0.8667606814803521 7.92773564050768E-4 54-55 0.0 0.0 0.0 0.9865609635247932 8.232648549757975E-4 56-57 0.0 0.0 0.0 1.12246064717765 8.53756145900827E-4 58-59 0.0 0.0 0.0 1.2659679079163013 8.842474368258566E-4 60-61 0.0 0.0 0.0 1.4206959637153638 8.842474368258566E-4 62-63 0.0 0.0 0.0 1.5726798033311735 9.452300186759157E-4 64-65 0.0 0.0 0.0 1.7264321378206349 9.452300186759157E-4 66-67 0.0 0.0 0.0 1.9040896443953197 9.757213096009452E-4 68-69 0.0 0.0 0.0 2.1009109273163853 0.0010062126005259747 70-71 0.0 0.0 0.0 2.2997903723748903 0.0010062126005259747 72-73 0.0 0.0 0.0 2.511918283340321 0.0011434234096886077 74-75 0.0 0.0 0.0 2.7171551625566943 0.0011586690551511224 76-77 0.0 0.0 0.0 2.938156039181309 0.0011739147006136373 78-79 0.0 0.0 0.0 3.1865380950565996 0.001189160346076152 80-81 0.0 0.0 0.0 3.446339139383314 0.0012044059915386667 82-83 0.0 0.0 0.0 3.7163090292335252 0.0013111255097762702 84-85 0.0 0.0 0.0 3.9924838967869807 0.0013111255097762702 86-87 0.0 0.0 0.0 4.273445897015665 0.0013111255097762702 88-89 3.049129092502954E-5 0.0 0.0 4.56931813850669 0.0013111255097762702 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6985 0.0 56.012043 1 GTATCAA 13490 0.0 46.212822 1 TATCAAC 17545 0.0 35.209564 2 ATCAACG 17645 0.0 34.92923 3 TCAACGC 17960 0.0 34.39598 4 CAACGCA 18160 0.0 33.991005 5 AACGCAG 18560 0.0 33.386456 6 TGGTATC 2880 0.0 32.174576 2 GTGGTAT 3180 0.0 30.194847 1 ACGCAGA 20995 0.0 29.32402 7 CGCAGAG 21140 0.0 29.077908 8 GCAGAGT 22965 0.0 26.207378 9 AGGTAAG 2165 0.0 25.680214 2 TAAGGTG 2490 0.0 25.381832 5 GTAAGGT 2465 0.0 23.90427 4 GTACATG 11310 0.0 23.864359 1 TACATGG 11020 0.0 23.414719 2 GAGTACT 15720 0.0 23.25361 12-13 AAGGTAA 2370 0.0 22.978434 1 GGTAAGG 2495 0.0 22.855007 3 >>END_MODULE