FastQCFastQC Report
Thu 26 May 2016
SRR937981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937981_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3279625
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176570.5383847238632467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143200.436635286046423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93830.28609978274955217No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA64110.19547966612036438No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA63740.19435148835613827No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA58540.17849601707512291No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA58470.1782825780386477No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC54090.16492739261348477No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG47850.14590082707626634No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA46100.14056485116438616No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA42410.12931356481305029No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA39490.12041010786294165No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA37120.11318367191370965No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35140.1071463963105538No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG35010.10675000952852841No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT33850.10321301978122499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71950.056.8458631
GTATCAA132050.043.456531
TATCAAC167550.034.0789762
ATCAACG168750.033.804883
TCAACGC171550.033.223924
CAACGCA172250.033.0888985
AACGCAG178150.032.0197146
ACGCAGA204950.027.7400057
CGCAGAG205400.027.6329828
TAAGGTG25050.027.49295
TGGTATC29350.026.8674532
AGGTAAG22800.026.043642
GTAAGGT25900.025.857094
GTGGTAT33550.024.7783321
GCAGAGT229050.024.4480259
AAGGTAA23200.023.9565561
GGTAAGG27400.023.4025613
GAGTACT154500.022.7951812-13
CAGAGTA215650.021.94748910-11
GTGTAAG13500.021.816471
Produced by FastQC (version 0.11.5)