##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937981_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3279625 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.531932156877694 34.0 31.0 34.0 31.0 34.0 2 32.743235888249416 34.0 31.0 34.0 31.0 34.0 3 32.79992499142433 34.0 31.0 34.0 31.0 34.0 4 36.252483744330526 37.0 35.0 37.0 35.0 37.0 5 36.216767465792586 37.0 37.0 37.0 35.0 37.0 6 36.25856980599916 37.0 37.0 37.0 35.0 37.0 7 36.22428539848306 37.0 37.0 37.0 35.0 37.0 8 36.2153670008004 37.0 37.0 37.0 35.0 37.0 9 37.93412935930175 39.0 38.0 39.0 35.0 39.0 10-11 37.91909623813699 39.0 38.0 39.0 35.0 39.0 12-13 37.87046567824065 39.0 38.0 39.0 35.0 39.0 14-15 39.201334451347336 40.5 39.0 41.0 36.0 41.0 16-17 39.15836063574342 40.0 39.0 41.0 36.0 41.0 18-19 39.1907128101536 40.0 39.0 41.0 36.0 41.0 20-21 39.121582955368375 40.0 39.0 41.0 36.0 41.0 22-23 38.98845066127987 40.0 38.5 41.0 35.0 41.0 24-25 38.93847162404238 40.0 38.0 41.0 35.0 41.0 26-27 38.89158150703206 40.0 38.0 41.0 35.0 41.0 28-29 38.832628577962424 40.0 38.0 41.0 35.0 41.0 30-31 38.61994862217479 40.0 38.0 41.0 35.0 41.0 32-33 38.420986240804964 40.0 38.0 41.0 34.0 41.0 34-35 38.21009673362046 40.0 38.0 41.0 34.0 41.0 36-37 38.060351564584366 40.0 38.0 41.0 33.5 41.0 38-39 38.021009414186075 40.0 38.0 41.0 33.0 41.0 40-41 37.94310401341617 40.0 38.0 41.0 33.0 41.0 42-43 37.76812272744597 40.0 37.5 41.0 33.0 41.0 44-45 37.54996196211457 40.0 37.0 41.0 33.0 41.0 46-47 37.20843373861341 40.0 37.0 41.0 32.0 41.0 48-49 36.828853908602355 40.0 36.0 41.0 31.0 41.0 50-51 36.5066486259862 40.0 36.0 41.0 30.5 41.0 52-53 36.08109494225712 39.0 35.0 41.0 29.5 41.0 54-55 35.848411632427485 39.0 35.0 41.0 29.0 41.0 56-57 35.58765072226245 39.0 35.0 40.0 28.0 41.0 58-59 35.23165163700118 38.0 35.0 40.0 27.5 41.0 60-61 34.86289941685406 38.0 34.0 40.0 27.0 41.0 62-63 34.491800434500895 37.0 34.0 40.0 26.0 41.0 64-65 34.093146167625875 36.5 34.0 39.5 26.0 41.0 66-67 33.677937569081834 36.0 33.5 39.0 25.5 41.0 68-69 33.216608301253956 35.5 33.0 39.0 24.5 40.0 70-71 32.70444502039105 35.0 32.5 38.0 22.5 40.0 72-73 32.30375302054351 35.0 32.0 37.0 22.0 39.5 74-75 31.877689217517247 35.0 32.0 37.0 20.5 39.0 76-77 30.580583145938938 34.0 30.0 35.5 20.0 38.0 78-79 31.29349879940542 34.5 31.5 36.0 20.0 38.0 80-81 31.147013301825666 35.0 32.0 35.5 19.5 37.0 82-83 30.793459160727217 35.0 31.0 35.0 18.0 37.0 84-85 30.435041811182682 34.0 31.0 35.0 14.5 36.5 86-87 30.033949613141747 34.0 31.0 35.0 8.5 36.0 88-89 29.580414834013034 34.0 30.0 35.0 4.5 36.0 90-91 29.253460990204672 34.0 30.0 35.0 2.0 35.0 92-93 28.869710408964437 34.0 29.0 35.0 2.0 35.0 94-95 28.47337348019972 34.0 29.0 35.0 2.0 35.0 96-97 27.92436452338301 34.0 29.0 35.0 2.0 35.0 98-99 27.254376796127605 33.5 27.0 35.0 2.0 35.0 100-101 24.79351861874452 31.0 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1593068768253474 1101 2 0.15823716805078902 1101 3 0.10405747152768896 1101 4 0.04900927474987071 1101 5 0.1276607887637553 1101 6 0.09408986330387137 1101 7 0.1279449213767947 1101 8 0.12409235140949448 1101 9 0.10504862073893406 1101 10-11 0.1343971165804092 1101 12-13 0.18401652199302987 1101 14-15 0.21421073577933214 1101 16-17 0.18454142087447423 1101 18-19 0.23264204262972044 1101 20-21 0.1978162285923446 1101 22-23 0.32987085684746376 1101 24-25 0.26307428742346417 1101 26-27 0.2618921763885069 1101 28-29 0.28640724864040124 1101 30-31 0.32201332879677835 1101 32-33 0.34320504783078576 1101 34-35 0.28499648625514595 1101 36-37 0.38316363322282854 1101 38-39 0.36387203453870853 1101 40-41 0.33976293038049477 1101 42-43 0.41563752330238657 1101 44-45 0.43132025090907433 1101 46-47 0.34564828847781115 1101 48-49 0.40968601840899765 1101 50-51 0.473657544917927 1101 52-53 0.30165383243156185 1101 54-55 0.42121377649259273 1101 56-57 0.4413783737388499 1101 58-59 0.3902864790048284 1101 60-61 0.44569035374530586 1101 62-63 0.45475485517962255 1101 64-65 0.40597315670016343 1101 66-67 0.35638391027035254 1101 68-69 0.3573591193625347 1101 70-71 0.45817353651212755 1101 72-73 0.39643927213354146 1101 74-75 0.4131961322760951 1101 76-77 0.356321669867139 1101 78-79 0.4694195904953169 1101 80-81 0.5838093714522756 1101 82-83 0.6434076941771316 1101 84-85 0.5681601041680047 1101 86-87 0.7559196791711145 1101 88-89 0.6692045100664714 1101 90-91 0.6966432920320251 1101 92-93 0.7891566963231682 1101 94-95 0.7187594284082301 1101 96-97 0.7051559620566543 1101 98-99 0.8637115970959641 1101 100-101 1.101020105009212 1102 1 0.1491222685785658 1102 2 0.09048339190634636 1102 3 0.12604211101470497 1102 4 0.04187792229480891 1102 5 0.034594359764021476 1102 6 0.03546065427568834 1102 7 0.07795578701227157 1102 8 0.08887143678454379 1102 9 0.032036317026211236 1102 10-11 0.024265345388933213 1102 12-13 0.12202837714689707 1102 14-15 0.18886245488419462 1102 16-17 0.14599255399280509 1102 18-19 0.20821954076642868 1102 20-21 0.10088149891689113 1102 22-23 0.13876602338747546 1102 24-25 0.055514249592967246 1102 26-27 -0.027180049898312575 1102 28-29 -0.034258004475134385 1102 30-31 0.06884377770519023 1102 32-33 0.052877467865691585 1102 34-35 0.17610350750901915 1102 36-37 0.10819632179660488 1102 38-39 0.12250416469948533 1102 40-41 0.21554054443360826 1102 42-43 0.22491274263789052 1102 44-45 0.178339534539802 1102 46-47 0.16504908229254056 1102 48-49 0.26849802433731185 1102 50-51 0.3048943035705278 1102 52-53 0.1437232263197643 1102 54-55 0.27118471183435844 1102 56-57 0.29835856063755983 1102 58-59 0.20316703597070074 1102 60-61 0.2652328041575558 1102 62-63 0.12870787358959745 1102 64-65 0.19349302712409866 1102 66-67 0.16539842768824542 1102 68-69 0.31603872098360597 1102 70-71 0.32628024414146495 1102 72-73 0.26024824815067404 1102 74-75 0.37461389848233395 1102 76-77 0.34485927999934063 1102 78-79 0.32943078857768526 1102 80-81 0.2947428896521487 1102 82-83 0.41307809997500655 1102 84-85 0.2661711550937369 1102 86-87 0.289358458465248 1102 88-89 0.13287222060007053 1102 90-91 0.15241520559876776 1102 92-93 0.23549335159945173 1102 94-95 0.21383169771758403 1102 96-97 0.41968186223605386 1102 98-99 0.24819381962075582 1102 100-101 0.4841609287783868 1103 1 0.04202366153062087 1103 2 0.09489118054865742 1103 3 0.10432870008627759 1103 4 0.04167305242503261 1103 5 0.07340700900514463 1103 6 0.07260965302224776 1103 7 0.07749759595615302 1103 8 0.08530595564550225 1103 9 0.06693139779665103 1103 10-11 0.06778167406496038 1103 12-13 0.04896641233885646 1103 14-15 0.09106632591145569 1103 16-17 0.1717787290152657 1103 18-19 0.20318811612767718 1103 20-21 0.11773807300349404 1103 22-23 0.055557933601591 1103 24-25 0.13093780844000236 1103 26-27 0.04706041165638197 1103 28-29 0.01375824022638028 1103 30-31 0.06188105563568769 1103 32-33 0.13306876437867032 1103 34-35 0.06459029835237118 1103 36-37 0.035056846529023744 1103 38-39 0.01759064214667916 1103 40-41 0.01760165878128106 1103 42-43 -0.029405946828539697 1103 44-45 0.03591386130526786 1103 46-47 0.05974538687801356 1103 48-49 0.11391281768894856 1103 50-51 0.1272355550447415 1103 52-53 0.20722607248520575 1103 54-55 0.19638001373381542 1103 56-57 0.20784975426136043 1103 58-59 0.23704685201410314 1103 60-61 0.23928304770694808 1103 62-63 0.23825798913900087 1103 64-65 0.044293195958310605 1103 66-67 0.09872627142529211 1103 68-69 0.302773600736991 1103 70-71 0.2567604665702774 1103 72-73 0.0951476499399746 1103 74-75 0.13126424932343284 1103 76-77 0.20019581981595636 1103 78-79 0.23476215714801185 1103 80-81 0.26843213595232385 1103 82-83 0.3056083045956335 1103 84-85 0.3898259031247626 1103 86-87 0.375352790127085 1103 88-89 0.22597777295789356 1103 90-91 0.14238939362574854 1103 92-93 0.291833031667025 1103 94-95 0.018652906536008373 1103 96-97 0.1003455823793864 1103 98-99 0.2798338687336219 1103 100-101 0.1746383699152787 1104 1 0.04914758229265459 1104 2 0.048738056017576525 1104 3 0.04595345756737146 1104 4 -0.008503837256135682 1104 5 0.040754973186487575 1104 6 0.006372854388239091 1104 7 -0.014407924324480348 1104 8 0.043066967063850825 1104 9 0.028297650062008017 1104 10-11 0.031235330023044128 1104 12-13 0.04915266114617367 1104 14-15 0.0818087550704476 1104 16-17 0.1593464151602646 1104 18-19 0.15450280971406016 1104 20-21 0.08610296336220813 1104 22-23 0.11935350524097998 1104 24-25 0.08136179223333073 1104 26-27 0.09404904690904203 1104 28-29 0.08501694602620091 1104 30-31 0.08471627987133701 1104 32-33 0.10775425050508147 1104 34-35 0.18009548135294295 1104 36-37 0.24849896835277008 1104 38-39 0.1396470486772401 1104 40-41 0.20807876989585594 1104 42-43 0.2462497027991759 1104 44-45 0.18694851709484084 1104 46-47 0.08881700770070466 1104 48-49 0.11202209844889666 1104 50-51 0.1587936056837691 1104 52-53 0.07845531681046936 1104 54-55 0.02929044711628137 1104 56-57 0.027092183530200487 1104 58-59 0.040317361297759646 1104 60-61 0.022714296686729085 1104 62-63 0.004926131442537951 1104 64-65 0.11870028103650299 1104 66-67 0.044828030611462566 1104 68-69 0.16121972875006207 1104 70-71 0.09824900043241058 1104 72-73 0.03286557642338295 1104 74-75 -0.0396114243523904 1104 76-77 -0.01971382067716121 1104 78-79 0.12995708072283563 1104 80-81 0.16472120948012048 1104 82-83 0.25717142146585203 1104 84-85 0.3372258565927666 1104 86-87 0.1776283979375215 1104 88-89 0.14554514625323378 1104 90-91 0.031169361229110848 1104 92-93 0.15989989097936785 1104 94-95 0.14018797019454254 1104 96-97 0.22843318775468902 1104 98-99 0.22195893302268743 1104 100-101 0.40329187287581547 1105 1 0.06312903631723543 1105 2 0.06392806431049536 1105 3 0.03577518500686949 1105 4 0.0338702486383653 1105 5 0.013924726660718534 1105 6 0.016492121596037634 1105 7 0.025903989831270735 1105 8 0.014971673921841955 1105 9 -0.005988782225074374 1105 10-11 0.03406401848584295 1105 12-13 0.0670454563614129 1105 14-15 0.0886892114321185 1105 16-17 0.0533374688631838 1105 18-19 0.04371473140883353 1105 20-21 0.06609004651753736 1105 22-23 0.05507622029136883 1105 24-25 0.049986072885708666 1105 26-27 0.05403190821630943 1105 28-29 -0.007304819108369998 1105 30-31 0.07198368871208771 1105 32-33 0.04589241550898038 1105 34-35 0.046400861696220375 1105 36-37 0.09917210181845348 1105 38-39 0.04789885175818398 1105 40-41 0.05234585204598119 1105 42-43 -0.04832436801729756 1105 44-45 0.06184671527039853 1105 46-47 0.09348456229573543 1105 48-49 0.23664014684937484 1105 50-51 0.056642724875580086 1105 52-53 0.13183731867008674 1105 54-55 0.06510227331686735 1105 56-57 -0.022898267051552068 1105 58-59 -0.017466520289453058 1105 60-61 0.07160284492385927 1105 62-63 0.058050550310177584 1105 64-65 -0.03706565769644499 1105 66-67 -0.039044459524987474 1105 68-69 -0.018698749374230772 1105 70-71 0.13577127821996982 1105 72-73 -8.775944860772711E-4 1105 74-75 -0.054556867018188626 1105 76-77 0.017037266197412748 1105 78-79 0.12925163001542828 1105 80-81 0.07740570172749983 1105 82-83 0.218484617254056 1105 84-85 0.24386511475374917 1105 86-87 0.23603118234683862 1105 88-89 0.047725328697382 1105 90-91 0.0924541600630775 1105 92-93 0.09597673866614542 1105 94-95 -0.06397592797850393 1105 96-97 0.09764008343985964 1105 98-99 0.230260644378955 1105 100-101 0.490904367535677 1106 1 -0.09030664149531731 1106 2 -0.03904918114212563 1106 3 -0.02482448085189759 1106 4 -0.01955865222522135 1106 5 -0.025395131211602973 1106 6 -0.04940909496042423 1106 7 0.015507303006323525 1106 8 -0.06658975394365285 1106 9 -0.10506777603640671 1106 10-11 -0.05031365523230846 1106 12-13 -0.16024663484502355 1106 14-15 -0.3104925190429739 1106 16-17 -0.20941034862982377 1106 18-19 -0.17541579545188313 1106 20-21 -0.1168088404627241 1106 22-23 -0.11412880594578212 1106 24-25 -0.10087196415219779 1106 26-27 -0.10482264622282145 1106 28-29 -0.011692422939226788 1106 30-31 -0.08427741207149353 1106 32-33 -0.10306228717592347 1106 34-35 -0.03377679790327903 1106 36-37 -0.062445047627392114 1106 38-39 -0.07955381548891438 1106 40-41 -0.08326795598753023 1106 42-43 -0.19943870549995069 1106 44-45 -0.1085746321188239 1106 46-47 -0.031814729418975674 1106 48-49 -0.056847722882615415 1106 50-51 -0.14539953693113006 1106 52-53 -0.23808691127555193 1106 54-55 -0.44269215923131355 1106 56-57 -0.24937707732051706 1106 58-59 -0.23042447026239188 1106 60-61 -0.16500769229054413 1106 62-63 -0.0725685522147117 1106 64-65 -0.15613462727076666 1106 66-67 -0.2448683529762974 1106 68-69 -0.39431622825510004 1106 70-71 -0.4409187469582818 1106 72-73 -0.3509724717715308 1106 74-75 -0.24828828335346032 1106 76-77 -0.21453540952087025 1106 78-79 -0.2886463863151363 1106 80-81 -0.5003095722918403 1106 82-83 -0.499684759407824 1106 84-85 -0.2955961178038429 1106 86-87 -0.24307647231113805 1106 88-89 -0.1903320400580597 1106 90-91 -0.31768006117606973 1106 92-93 -0.1681918850014057 1106 94-95 -0.18150125917835425 1106 96-97 -0.08872954265139654 1106 98-99 0.020712911597819073 1106 100-101 -0.21847643581514475 1107 1 0.069560156527821 1107 2 0.09316234333945062 1107 3 0.03337448304723267 1107 4 0.10367545483901353 1107 5 0.06705371174408015 1107 6 0.09244766415234551 1107 7 0.05124590267128326 1107 8 0.1205194949221351 1107 9 0.03550873166787483 1107 10-11 0.10570894390262708 1107 12-13 0.11420427472936012 1107 14-15 0.2006511886184299 1107 16-17 0.18485604154525248 1107 18-19 0.16333157844013613 1107 20-21 0.16358697544411882 1107 22-23 0.17117390967422352 1107 24-25 0.14975852096994657 1107 26-27 -0.003948202927297473 1107 28-29 0.028401527776679814 1107 30-31 0.07550635615871215 1107 32-33 0.14505708354056424 1107 34-35 0.15085538056138859 1107 36-37 0.07998871486320525 1107 38-39 0.12230306351600717 1107 40-41 0.18078691668475955 1107 42-43 0.13887598296251724 1107 44-45 0.12774551608935525 1107 46-47 0.08257844939722503 1107 48-49 0.06683669554782057 1107 50-51 0.040079402266634645 1107 52-53 0.13985428269466382 1107 54-55 0.1047395941709226 1107 56-57 0.0702313029981596 1107 58-59 0.04629539840022545 1107 60-61 -0.07213099538032708 1107 62-63 0.09612016130754597 1107 64-65 0.02655001940212287 1107 66-67 0.03254196925535524 1107 68-69 0.08114564957282511 1107 70-71 0.09640538175900559 1107 72-73 0.12707970296931848 1107 74-75 0.14093655211021527 1107 76-77 0.07863773417050623 1107 78-79 0.2554218552274783 1107 80-81 0.3700403902329157 1107 82-83 0.4091286652212993 1107 84-85 0.4169952697698385 1107 86-87 0.6100510712032268 1107 88-89 0.53835182767369 1107 90-91 0.5854936452926793 1107 92-93 0.6468401490200506 1107 94-95 0.46913375321482675 1107 96-97 0.7587836926823286 1107 98-99 0.8416821126445306 1107 100-101 0.9862211268220236 1108 1 0.02493560401853756 1108 2 0.029755324769332958 1108 3 -0.054007091196453416 1108 4 -0.01163672392123516 1108 5 -0.014902983640673995 1108 6 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-0.011125114655278878 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 11.0 4 27.0 5 40.0 6 110.0 7 290.0 8 779.0 9 1449.0 10 2447.0 11 4012.0 12 6121.0 13 8969.0 14 12213.0 15 16463.0 16 21172.0 17 30629.0 18 30443.0 19 22300.0 20 18278.0 21 17622.0 22 17990.0 23 19131.0 24 21334.0 25 24739.0 26 28904.0 27 34789.0 28 42394.0 29 51927.0 30 63849.0 31 79018.0 32 97850.0 33 128684.0 34 182588.0 35 285274.0 36 484531.0 37 782779.0 38 664357.0 39 76103.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.414994092312384 18.616884552349735 14.85861950680337 23.10950184853451 2 15.900435604143057 19.704469852314215 39.607468336055604 24.787626207487122 3 19.376732028375994 23.424768981567315 29.949789473170636 27.248709516886056 4 13.07917825970957 16.530243549186263 36.161969737393754 34.22860845371041 5 13.616861683881542 37.14580173038076 34.167839310896824 15.069497274840874 6 33.00377329725197 36.004207798147654 16.356931051568395 14.635087853031978 7 28.98471624042383 33.0536265579144 19.68737279414567 18.274284407516102 8 27.151471391772695 34.32249385293888 19.034789438763564 19.49124531652486 9 26.45937366314094 16.50221108674845 19.794981657874462 37.243433592236144 10-11 25.31906849106224 26.230026298738423 26.52544117086557 21.925464039333765 12-13 26.45404581316462 23.63280862903533 27.110828219689754 22.802317338110303 14-15 22.784449441628237 24.955688531463203 25.749956168769295 26.50990585813927 16-17 22.120210018329875 28.2636074897126 26.84342706323922 22.772755428718312 18-19 22.281083965392384 26.23591111788695 29.525113389488126 21.957891527232533 20-21 22.34677745169036 25.3925372565461 30.26814041239471 21.99254487936883 22-23 22.349064298509738 25.3438121736479 30.190951709417995 22.116171818424363 24-25 22.24202058117933 25.608055003083596 30.092294369258283 22.057630046478796 26-27 21.495952167104058 26.08277433236299 29.9067984378979 22.514475062635046 28-29 21.8557218754345 26.426703183047813 29.737844948079413 21.979729993438273 30-31 21.854587033578536 25.893951290162747 29.604024850402105 22.647436825856616 32-33 21.434189477189122 25.951405480257783 29.716290831664814 22.89811421088828 34-35 22.526365580143406 26.08822348094277 29.414170395710144 21.971240543203688 36-37 22.730468891742305 25.45323233823215 29.362228803850833 22.45406996617471 38-39 21.964880564092102 25.919850297602437 29.27412574232877 22.841143395976687 40-41 22.61140183951792 25.697885408280268 29.400936836712287 22.28977591548952 42-43 22.205774166964616 25.767534547650108 29.205459317745376 22.821231967639903 44-45 22.118111759628935 26.13475500067921 29.496304271695074 22.25082896799678 46-47 22.803296535929647 26.59549896642131 28.341415305319252 22.259789192329794 48-49 22.362678659907765 26.662286084537108 28.25060792011282 22.724427335442314 50-51 22.699897091893128 26.91281777642261 28.212097419674503 22.17518771200976 52-53 23.179723291534856 26.371185730075847 28.193589206083015 22.255501772306285 54-55 22.333346038037885 26.828524602660366 28.45807066356672 22.38005869573503 56-57 22.426542668750237 26.954499371117123 28.189305179708047 22.42965278042459 58-59 22.467035103098677 26.393230933414642 28.059686701985747 23.080047261500933 60-61 21.872790334260777 26.875648892784998 28.468773106681404 22.78278766627282 62-63 22.152730876243474 26.877356405076803 28.619476312078362 22.350436406601364 64-65 21.749422571178105 26.96896748866105 28.37830544650684 22.903304493654 66-67 21.61301978122499 26.902359263635322 28.438480009147387 23.046140945992303 68-69 22.42197977841603 26.473050414919193 28.563052151403774 22.541917655261006 70-71 22.123535465182755 26.80594580173038 27.865670617829778 23.20484811525708 72-73 22.037214620574 26.93448183862484 27.919579220185238 23.108724320615927 74-75 21.997408240271373 27.351755154933873 27.91034035903495 22.740496245759807 76-77 22.134619049434008 26.742645881770024 28.186362770133783 22.936372298662196 78-79 22.26463391393833 27.072485421351526 28.02703052940504 22.635850135305102 80-81 22.471212409955406 27.135084041620612 27.669703091054615 22.724000457369364 82-83 22.19185796908426 27.245074435362106 27.835965342368514 22.727102253185123 84-85 22.160571949466394 27.396992944547836 27.759286761586033 22.683148344399736 86-87 22.407659074409082 27.399635261843397 27.302901287438488 22.889804376309034 88-89 22.178304586135482 27.60726534505175 27.105561491195335 23.108868577617432 90-91 22.007998490068513 27.525514396641803 27.322960380458827 23.143526732830857 92-93 22.12953862136015 27.019759779840115 27.673266066322082 23.177435532477652 94-95 21.85620307199756 26.800853756145898 27.83505736174105 23.507885810115486 96-97 22.168211965761927 26.365583371752372 27.877296299819736 23.588908362665965 98-99 21.791317604909096 26.801920951328277 27.448824179593707 23.957937264168923 100-101 21.786515226588403 26.438419026565533 27.929839539581508 23.84522620726455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1311.0 1 912.0 2 771.5 3 1025.5 4 823.0 5 776.0 6 1604.0 7 3696.0 8 6082.0 9 6888.5 10 5535.5 11 4029.0 12 3861.5 13 4022.5 14 4195.0 15 4439.0 16 4677.0 17 4982.5 18 5448.0 19 6023.5 20 6731.0 21 7942.0 22 9429.5 23 11275.5 24 13789.0 25 17166.5 26 21476.0 27 26396.0 28 30925.0 29 35000.0 30 39076.5 31 45858.5 32 58460.5 33 62408.0 34 58392.0 35 60236.0 36 65823.0 37 71357.5 38 75606.0 39 79838.0 40 84682.0 41 91579.5 42 95628.0 43 98920.5 44 103605.5 45 110406.0 46 113324.5 47 117035.0 48 119990.5 49 115916.0 50 115912.5 51 115627.5 52 113115.0 53 115213.5 54 120634.5 55 114843.5 56 104630.0 57 97085.5 58 91273.5 59 81792.0 60 64038.5 61 53195.5 62 47080.0 63 33979.0 64 22817.5 65 18098.0 66 13549.5 67 10338.0 68 7942.5 69 5900.5 70 4397.5 71 2840.0 72 1887.5 73 1499.5 74 1093.5 75 744.0 76 531.5 77 321.5 78 200.0 79 139.5 80 74.5 81 37.0 82 26.0 83 19.5 84 12.5 85 6.5 86 2.5 87 2.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0152761367534398 3 0.0071044707855318825 4 0.0 5 0.0 6 0.0 7 0.0 8 2.7442161832526583E-4 9 4.8786065480047264E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.98193391012692E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005259747684567596 26-27 0.02164881655677097 28-29 3.049129092502954E-5 30-31 0.0 32-33 5.031063002629873E-4 34-35 0.01933147844646873 36-37 0.003841902656553722 38-39 0.01786789648206731 40-41 0.015398101917139917 42-43 0.008461333231695697 44-45 0.002911918283340321 46-47 1.524564546251477E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.2015855471281016E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.049129092502954E-5 84-85 0.0031863399016655867 86-87 3.658954911003544E-4 88-89 3.049129092502954E-5 90-91 1.829477455501772E-4 92-93 2.591759728627511E-4 94-95 0.0 96-97 3.049129092502954E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3279625.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.928883262020854 #Duplication Level Percentage of deduplicated Percentage of total 1 69.46263259345618 30.514158782705813 2 15.576686027803824 13.68532844249091 3 6.001646989412794 7.9093694993332075 4 2.864115839226672 5.032696414011735 5 1.6419391855196908 3.606427740201605 6 0.9995795030433342 2.6346246780179667 7 0.6709581288955533 2.063210892256969 8 0.4817704762632821 1.6930911208686328 9 0.35870182959207814 1.4181633718221327 >10 1.7411125565546712 13.550634611905513 >50 0.1196135732364969 3.5806078110583193 >100 0.06906078968105342 5.721970895162255 >500 0.006921879156597999 2.0633914726269094 >1k 0.004706877825354626 4.359670480202501 >5k 4.153127492959963E-4 1.1943278009819835 >10k+ 1.3843758309866545E-4 0.9723259863537066 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17657 0.5383847238632467 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14320 0.436635286046423 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9383 0.28609978274955217 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6411 0.19547966612036438 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6374 0.19435148835613827 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5854 0.17849601707512291 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5847 0.1782825780386477 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5409 0.16492739261348477 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4785 0.14590082707626634 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4610 0.14056485116438616 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4241 0.12931356481305029 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3949 0.12041010786294165 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3712 0.11318367191370965 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3514 0.1071463963105538 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3501 0.10675000952852841 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3385 0.10321301978122499 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.098258185005908E-5 2 0.0 0.0 0.0 0.0 6.098258185005908E-5 3 0.0 0.0 0.0 0.0 6.098258185005908E-5 4 0.0 0.0 0.0 0.0 9.147387277508862E-5 5 0.0 0.0 0.0 0.0 9.147387277508862E-5 6 0.0 0.0 0.0 0.0 1.2196516370011816E-4 7 0.0 0.0 0.0 0.0 1.2196516370011816E-4 8 0.0 0.0 0.0 0.0 1.2196516370011816E-4 9 0.0 0.0 0.0 0.0 1.2196516370011816E-4 10-11 0.0 0.0 0.0 0.0 1.2196516370011816E-4 12-13 0.0 0.0 0.0 0.0 2.4393032740023632E-4 14-15 0.0 0.0 0.0 0.0 3.2015855471281016E-4 16-17 0.0 0.0 0.0 0.0 3.658954911003545E-4 18-19 0.0 0.0 0.0 1.524564546251477E-5 3.8114113656286924E-4 20-21 0.0 0.0 0.0 4.573693638754431E-5 4.2687807295041356E-4 22-23 0.0 0.0 0.0 1.3721080916263292E-4 4.8786065480047264E-4 24-25 0.0 0.0 0.0 4.116324274878988E-4 5.488432366505317E-4 26-27 0.0 0.0 0.0 0.001250142927926211 5.793345275755612E-4 28-29 0.0 0.0 0.0 0.004512711056904372 5.793345275755612E-4 30-31 0.0 0.0 0.0 0.014025993825513589 6.098258185005907E-4 32-33 0.0 0.0 0.0 0.03437893051797081 6.708084003506498E-4 34-35 0.0 0.0 0.0 0.0618363379959599 7.012996912756793E-4 36-37 0.0 0.0 0.0 0.10854899569310517 7.012996912756793E-4 38-39 0.0 0.0 0.0 0.17863322788428554 7.012996912756793E-4 40-41 0.0 0.0 0.0 0.2524526432137821 7.012996912756793E-4 42-43 0.0 0.0 0.0 0.3264092693524412 7.622822731257384E-4 44-45 0.0 0.0 0.0 0.41946868925563136 8.385105004383123E-4 46-47 0.0 0.0 0.0 0.5270877005755231 8.994930822883713E-4 48-49 0.0 0.0 0.0 0.637374699851355 9.757213096009453E-4 50-51 0.0 0.0 0.0 0.759187407096848 9.757213096009453E-4 52-53 0.0 0.0 0.0 0.8710599535007814 0.0010519495369135192 54-55 0.0 0.0 0.0 0.9911803940999353 0.001067195182376034 56-57 0.0 0.0 0.0 1.1279185882532303 0.001067195182376034 58-59 0.0 0.0 0.0 1.2720051835194572 0.0010824408278385486 60-61 0.0 0.0 0.0 1.4272515912642452 0.0010976864733010633 62-63 0.0 0.0 0.0 1.579921484925868 0.001128177764226093 64-65 0.0 0.0 0.0 1.7341006974882798 0.0011586690551511226 66-67 0.0 0.0 0.0 1.913343751191066 0.001189160346076152 68-69 0.0 0.0 0.0 2.111415177040058 0.001189160346076152 70-71 0.0 0.0 0.0 2.3117277127720395 0.001189160346076152 72-73 0.0 0.0 0.0 2.524983801501696 0.001387353737088844 74-75 0.0 0.0 0.0 2.7312878759004455 0.0014788276098639326 76-77 0.0 0.0 0.0 2.953737088843999 0.0016007927735640507 78-79 0.0 0.0 0.0 3.2047261500933795 0.00166177535541411 80-81 0.0 0.0 0.0 3.4678964820673097 0.0017380035827266836 82-83 0.0 0.0 0.0 3.74192171361055 0.0018294774555017723 84-85 0.0 0.0 0.0 4.022456835766285 0.0018294774555017723 86-87 0.0 0.0 0.0 4.306620421542097 0.0018752143918893166 88-89 0.0 0.0 0.0 4.606974882799101 0.001920951328276861 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7195 0.0 56.845863 1 GTATCAA 13205 0.0 43.45653 1 TATCAAC 16755 0.0 34.078976 2 ATCAACG 16875 0.0 33.80488 3 TCAACGC 17155 0.0 33.22392 4 CAACGCA 17225 0.0 33.088898 5 AACGCAG 17815 0.0 32.019714 6 ACGCAGA 20495 0.0 27.740005 7 CGCAGAG 20540 0.0 27.632982 8 TAAGGTG 2505 0.0 27.4929 5 TGGTATC 2935 0.0 26.867453 2 AGGTAAG 2280 0.0 26.04364 2 GTAAGGT 2590 0.0 25.85709 4 GTGGTAT 3355 0.0 24.778332 1 GCAGAGT 22905 0.0 24.448025 9 AAGGTAA 2320 0.0 23.956556 1 GGTAAGG 2740 0.0 23.402561 3 GAGTACT 15450 0.0 22.79518 12-13 CAGAGTA 21565 0.0 21.947489 10-11 GTGTAAG 1350 0.0 21.81647 1 >>END_MODULE