FastQCFastQC Report
Thu 26 May 2016
SRR937980_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937980_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3315369
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174840.5273621126336164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137530.4148256197123156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87490.263892194202214No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA64130.19343246558678687No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA62280.1878523929010617No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA58730.17714468585548093No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA54500.16438592506595795No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC51530.1554276462137397No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG47520.14333246163549218No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA46250.13950181714312948No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA43390.13087532639654892No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA39830.12013745679591019No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT35570.10728820834121329No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG34110.10288447530274909No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33930.10234154931170558No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA33640.10146683521502432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74650.056.6345521
GTATCAA141250.043.818071
TATCAAC179850.034.2989652
ATCAACG179250.034.228043
TCAACGC181950.033.720124
CAACGCA183300.033.419885
AACGCAG188950.032.4645166
ACGCAGA214100.028.5095067
GTGGTAT33550.028.3815291
CGCAGAG214700.028.296138
TGGTATC31300.028.1111662
TAAGGTA12150.026.2270834
GCAGAGT233650.025.4921789
GTGTAAG14700.024.2908231
GTACATG118550.024.056011
TAAGGTG24800.023.9723035
GAGTACT153100.023.30115712-13
TACATGG118000.023.1759382
AGGTAAG22200.023.137812
AGAGTAC207250.022.38500610-11
Produced by FastQC (version 0.11.5)