##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937980_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3315369 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.993848648521478 33.0 31.0 34.0 30.0 34.0 2 32.107002568944814 34.0 31.0 34.0 30.0 34.0 3 32.128679190762774 34.0 31.0 34.0 30.0 34.0 4 35.64423055171234 37.0 35.0 37.0 35.0 37.0 5 35.65331219541475 37.0 35.0 37.0 35.0 37.0 6 35.68719319026027 37.0 36.0 37.0 35.0 37.0 7 35.62380778730814 37.0 35.0 37.0 33.0 37.0 8 35.60933941289793 37.0 35.0 37.0 33.0 37.0 9 37.25523735065388 39.0 37.0 39.0 34.0 39.0 10-11 37.16812125588434 39.0 37.0 39.0 34.0 39.0 12-13 37.025371082374235 39.0 37.0 39.0 33.0 39.0 14-15 38.215379494710845 40.0 38.0 41.0 33.0 41.0 16-17 38.051199881521484 40.0 38.0 41.0 33.0 41.0 18-19 38.22369923227249 40.0 38.0 41.0 33.5 41.0 20-21 38.218842005218725 40.0 38.0 41.0 34.0 41.0 22-23 38.11527419723114 40.0 38.0 41.0 33.0 41.0 24-25 37.99386508711399 40.0 38.0 41.0 33.0 41.0 26-27 37.811415561887685 40.0 38.0 41.0 33.0 41.0 28-29 37.6353255097698 40.0 38.0 41.0 32.0 41.0 30-31 37.39171899115905 40.0 37.0 41.0 32.0 41.0 32-33 37.08466915749047 40.0 36.5 41.0 31.0 41.0 34-35 36.836187465105695 39.5 36.0 41.0 30.5 41.0 36-37 36.553200111360155 39.0 35.5 41.0 30.0 41.0 38-39 36.24511012198039 39.0 35.0 41.0 30.0 41.0 40-41 36.11607079031022 39.0 35.0 41.0 29.5 41.0 42-43 35.87674644964105 39.0 35.0 41.0 28.5 41.0 44-45 35.54650809608222 39.0 35.0 40.0 27.0 41.0 46-47 35.1440642052212 38.5 35.0 40.0 25.5 41.0 48-49 34.709650569815906 38.0 34.0 40.0 24.0 41.0 50-51 33.94721733840185 37.5 33.5 39.5 23.0 40.5 52-53 33.99412931109629 38.0 33.5 40.0 23.0 40.5 54-55 34.069320639723664 38.0 34.0 40.0 22.5 41.0 56-57 33.747319378325614 38.0 33.5 40.0 20.5 41.0 58-59 33.33310741579595 37.0 33.0 40.0 18.5 41.0 60-61 32.83652815116507 37.0 32.5 40.0 14.0 41.0 62-63 32.30499953398852 36.0 31.5 39.0 9.0 41.0 64-65 31.773701509545393 35.5 31.0 39.0 7.0 40.0 66-67 31.23481096674307 35.0 30.5 38.5 2.0 40.0 68-69 30.579461592359703 35.0 29.5 38.0 2.0 40.0 70-71 30.000391359151877 34.5 29.0 37.0 2.0 39.5 72-73 29.337630743365217 34.0 28.5 36.5 2.0 39.0 74-75 28.69760982864954 34.0 27.5 36.0 2.0 39.0 76-77 27.98982375717454 34.0 26.0 35.5 2.0 38.0 78-79 27.25425405135899 33.0 25.5 35.0 2.0 37.0 80-81 26.571325544758366 32.5 23.5 35.0 2.0 36.5 82-83 25.85445436088713 32.0 21.0 35.0 2.0 36.0 84-85 25.165585037442288 31.5 19.0 35.0 2.0 35.5 86-87 24.456897407196607 31.0 14.0 34.0 2.0 35.0 88-89 23.586100370727962 30.5 4.5 34.0 2.0 35.0 90-91 22.71142880324935 30.0 2.0 34.0 2.0 35.0 92-93 21.802925405889965 29.0 2.0 34.0 2.0 35.0 94-95 20.742802384892904 29.0 2.0 34.0 2.0 35.0 96-97 19.509897239191172 26.5 2.0 33.5 2.0 35.0 98-99 17.966211604198506 24.0 2.0 33.0 2.0 35.0 100-101 14.014969977700822 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12040857456391763 1101 2 0.11566270134868262 1101 3 0.07911392635016057 1101 4 -0.07372210481872088 1101 5 0.054269085874096845 1101 6 -0.006829274238491223 1101 7 0.03762923350550551 1101 8 0.06232368928887411 1101 9 0.04033781828035643 1101 10-11 0.10671781102851696 1101 12-13 0.12939514426692966 1101 14-15 0.1600436477676439 1101 16-17 0.12317628633811495 1101 18-19 0.1516563989097719 1101 20-21 0.10937858209916129 1101 22-23 0.18089514642019822 1101 24-25 0.17146003347394156 1101 26-27 0.08979428441072201 1101 28-29 0.18403274204845843 1101 30-31 0.14950436073664974 1101 32-33 0.12393474382046321 1101 34-35 0.12562262643455568 1101 36-37 0.021351061876394795 1101 38-39 -0.012881621301815471 1101 40-41 0.17164211008465458 1101 42-43 0.2502055354146151 1101 44-45 0.14285949440953516 1101 46-47 0.21857018463423827 1101 48-49 0.30338677613487164 1101 50-51 0.2669301543567357 1101 52-53 0.32100577591496204 1101 54-55 0.2024294366244419 1101 56-57 0.27968984572688527 1101 58-59 0.3920031178113135 1101 60-61 0.32153290174624516 1101 62-63 0.17466826332550056 1101 64-65 0.11611144617585012 1101 66-67 0.1258644479053821 1101 68-69 0.05707042057447964 1101 70-71 0.04582779805712889 1101 72-73 0.14722373885919993 1101 74-75 0.03606046302117605 1101 76-77 -0.21457821382002606 1101 78-79 -0.314890143597907 1101 80-81 -0.1918397539985719 1101 82-83 -0.4075933288229763 1101 84-85 -0.5800230459126468 1101 86-87 -0.3907989851677023 1101 88-89 -0.3083965471153718 1101 90-91 -0.5872666932091626 1101 92-93 -0.732401909221295 1101 94-95 -0.9886785337853112 1101 96-97 -1.1350462413653872 1101 98-99 -1.142022587790354 1101 100-101 -1.1016482337036972 1102 1 0.11090163759465099 1102 2 0.23457790287960023 1102 3 0.17812196964641913 1102 4 0.17674397280357823 1102 5 0.0715475962197516 1102 6 0.08873682132423966 1102 7 0.08656439377341485 1102 8 0.07111936265330598 1102 9 0.14375285535726334 1102 10-11 0.22158034242428215 1102 12-13 0.2327513142824742 1102 14-15 0.3081258897243444 1102 16-17 0.196178543843196 1102 18-19 0.24164825096095655 1102 20-21 0.2335211786785223 1102 22-23 0.3177008888553132 1102 24-25 0.20676008729525108 1102 26-27 0.24214055728479167 1102 28-29 0.24072751540093407 1102 30-31 0.2332981653054773 1102 32-33 0.12577831067849132 1102 34-35 0.1972315099413109 1102 36-37 0.32650931148768336 1102 38-39 0.10678502293930592 1102 40-41 0.1673827511563033 1102 42-43 0.2511081636887482 1102 44-45 0.3837771478002807 1102 46-47 0.26381634239049134 1102 48-49 0.07720420228007185 1102 50-51 0.0913074716632778 1102 52-53 -0.0735496749880511 1102 54-55 0.02030016829529302 1102 56-57 0.0010933485973581014 1102 58-59 -0.11352178949642422 1102 60-61 0.033319208707133896 1102 62-63 -0.19294326639582948 1102 64-65 -0.36478404329909253 1102 66-67 -0.4091736007730056 1102 68-69 -0.5154132461519865 1102 70-71 -0.7181636877101276 1102 72-73 -0.6453365858627187 1102 74-75 -0.6273531911469838 1102 76-77 -0.9396490411732117 1102 78-79 -0.845985594201899 1102 80-81 -0.6712555311185326 1102 82-83 -0.5588921417640123 1102 84-85 -0.7411008924621036 1102 86-87 -0.7526402347191912 1102 88-89 -0.6035096466249996 1102 90-91 -0.6988558422344013 1102 92-93 -0.9747478830880567 1102 94-95 -1.1270148198156882 1102 96-97 -1.0497403942777161 1102 98-99 -1.0784512072736696 1102 100-101 -1.1148097873456067 1103 1 0.15479136065103916 1103 2 0.20813479310315586 1103 3 0.22180523624635384 1103 4 0.12442185902312985 1103 5 0.1288204941176474 1103 6 0.18794749032657876 1103 7 0.18505976414378722 1103 8 0.19086306468867775 1103 9 0.20688577994852153 1103 10-11 0.21118557265451443 1103 12-13 0.10774557938090368 1103 14-15 0.23124797013809228 1103 16-17 0.18779203233169284 1103 18-19 0.23498012450116335 1103 20-21 0.35126183600250727 1103 22-23 0.2803713718382994 1103 24-25 0.17320361415544738 1103 26-27 0.1013919962237253 1103 28-29 0.16734580035254965 1103 30-31 0.26976796844194695 1103 32-33 0.2750499781793323 1103 34-35 0.05906521865001224 1103 36-37 0.14333759810763524 1103 38-39 0.107860577660702 1103 40-41 0.19763383557405234 1103 42-43 0.2245451423340583 1103 44-45 0.22543130195442984 1103 46-47 0.25736301406215745 1103 48-49 0.2746224538649926 1103 50-51 0.38238396482310577 1103 52-53 0.42949190826187333 1103 54-55 0.2597151040643979 1103 56-57 0.20507701976852388 1103 58-59 0.3028675164762191 1103 60-61 0.2640035388706323 1103 62-63 0.11176143830887497 1103 64-65 0.009865084995869466 1103 66-67 -0.051852738243809426 1103 68-69 0.23050506799549453 1103 70-71 -0.03992659645220087 1103 72-73 -0.10380859954306842 1103 74-75 -0.17707947993577378 1103 76-77 -0.09791616246116774 1103 78-79 -0.04771805583019528 1103 80-81 -0.2524403617825328 1103 82-83 -0.4685611858080563 1103 84-85 -0.6552035367730831 1103 86-87 -0.7859866019197277 1103 88-89 -0.5916884504288049 1103 90-91 -0.6001706987575872 1103 92-93 -0.6738266493543215 1103 94-95 -0.7020111703328737 1103 96-97 -0.757414213930705 1103 98-99 -0.6571345781445395 1103 100-101 -0.8841228504086693 1104 1 0.24719955832240004 1104 2 0.26820363387374613 1104 3 0.26417216457819137 1104 4 0.21156163037342424 1104 5 0.28095508484873477 1104 6 0.2567619187901258 1104 7 0.24971213581370222 1104 8 0.20617662386188584 1104 9 0.3151080372935837 1104 10-11 0.28472402338594804 1104 12-13 0.2752332424826065 1104 14-15 0.28858600993228123 1104 16-17 0.24754915787828935 1104 18-19 0.35916571359903315 1104 20-21 0.40803791032244163 1104 22-23 0.5132753284264595 1104 24-25 0.43321801452072606 1104 26-27 0.33041926911064934 1104 28-29 0.2951622132566811 1104 30-31 0.36884072540944857 1104 32-33 0.32457318559726644 1104 34-35 0.2533779524364306 1104 36-37 0.19216273903803938 1104 38-39 0.16522495958648165 1104 40-41 0.2681868447235516 1104 42-43 0.18417143529368474 1104 44-45 0.1830380314909874 1104 46-47 0.01941453927158676 1104 48-49 0.12602648947954975 1104 50-51 0.1471838524300111 1104 52-53 0.14351294684431082 1104 54-55 -0.03326647400490401 1104 56-57 7.674470379015474E-4 1104 58-59 0.011677203356079247 1104 60-61 -0.0671346168289304 1104 62-63 -0.008797717337998279 1104 64-65 0.037677985615786724 1104 66-67 -0.057494344762606175 1104 68-69 -0.1698997227601744 1104 70-71 -0.1857654177296233 1104 72-73 -0.17134982497903195 1104 74-75 -0.31795280940559323 1104 76-77 -0.40661521238828513 1104 78-79 -0.583977999984878 1104 80-81 -0.5882805879736352 1104 82-83 -0.5897218201265844 1104 84-85 -0.5182025006492381 1104 86-87 -0.5560505254924237 1104 88-89 -0.4569879604651561 1104 90-91 -0.5610614140342705 1104 92-93 -0.5607442687280795 1104 94-95 -0.8245284244290758 1104 96-97 -0.9291517409313883 1104 98-99 -0.9919167287161628 1104 100-101 -1.1017615417315714 1105 1 0.18596393141089607 1105 2 0.1766885226591981 1105 3 0.2679729031762861 1105 4 0.18348903316716303 1105 5 0.2106376239448835 1105 6 0.2676641832249942 1105 7 0.23190003461008502 1105 8 0.30349043200124015 1105 9 0.243163476891624 1105 10-11 0.29691659742533716 1105 12-13 0.32173046340159317 1105 14-15 0.28042290839494655 1105 16-17 0.3864657534311817 1105 18-19 0.2961279221198012 1105 20-21 0.24017564979524053 1105 22-23 0.3827923335454244 1105 24-25 0.3001126352000725 1105 26-27 0.30142968394302727 1105 28-29 0.28640906239328245 1105 30-31 0.27278367102598367 1105 32-33 0.27762003612946984 1105 34-35 0.12671293585552945 1105 36-37 0.325096952745497 1105 38-39 0.2847142580414328 1105 40-41 0.32375163583377997 1105 42-43 0.35813398491426796 1105 44-45 0.28600656451958884 1105 46-47 0.37582259809344265 1105 48-49 0.36212646661642367 1105 50-51 0.2533358811529993 1105 52-53 0.3005306138446926 1105 54-55 0.4035066108797025 1105 56-57 0.32438805537773163 1105 58-59 0.33210579876777757 1105 60-61 0.3274492229206558 1105 62-63 0.43471167850485415 1105 64-65 0.26689685882065106 1105 66-67 0.1570892528102057 1105 68-69 0.07432066712800278 1105 70-71 0.13662624613559515 1105 72-73 0.0027860452175048067 1105 74-75 -0.05687004118558647 1105 76-77 -0.033885876120923086 1105 78-79 -0.2897993137568946 1105 80-81 -0.2685686716042497 1105 82-83 -0.20701521153308633 1105 84-85 -0.18664885870060743 1105 86-87 -0.3225026399624049 1105 88-89 -0.1573132982709602 1105 90-91 -0.25194750188204296 1105 92-93 -0.6433117693448125 1105 94-95 -0.7647564276980461 1105 96-97 -0.5562247637941091 1105 98-99 -0.4733557266461652 1105 100-101 -0.7439382880826031 1106 1 0.29612420488243885 1106 2 0.27098231971879727 1106 3 0.28853863631308485 1106 4 0.28168056700647526 1106 5 0.34651955964280035 1106 6 0.29016668674866963 1106 7 0.29053480440143176 1106 8 0.1579098215905077 1106 9 0.39237043644767056 1106 10-11 0.3753652803124865 1106 12-13 0.47636005306785023 1106 14-15 0.46794947495553885 1106 16-17 0.020732115271776763 1106 18-19 0.3809361219064371 1106 20-21 0.4967328764010901 1106 22-23 0.5975954281927898 1106 24-25 0.5148734413325187 1106 26-27 0.4266007453455174 1106 28-29 0.3692816906942369 1106 30-31 0.32101383128202343 1106 32-33 0.4386395511891905 1106 34-35 0.4243730730883044 1106 36-37 0.47438500161881336 1106 38-39 0.4535877748371746 1106 40-41 0.5140097352832882 1106 42-43 0.4424146681745995 1106 44-45 0.6710010953503129 1106 46-47 0.6357258489467341 1106 48-49 0.7136862588708226 1106 50-51 0.649576105203657 1106 52-53 0.5394787066544708 1106 54-55 0.5315029374464331 1106 56-57 0.39536202527092 1106 58-59 0.6178403568113779 1106 60-61 0.8123769640556517 1106 62-63 0.69915473222391 1106 64-65 0.4838820626360345 1106 66-67 0.3673640976396939 1106 68-69 0.3877171454974295 1106 70-71 0.24407427812838378 1106 72-73 0.2968627757659874 1106 74-75 0.3853462998813093 1106 76-77 0.008016374630788192 1106 78-79 0.11887918323080982 1106 80-81 0.2616259819939586 1106 82-83 0.32323993208001767 1106 84-85 0.5117092352121517 1106 86-87 0.5106480244250413 1106 88-89 0.11066607801267736 1106 90-91 0.0032469010368245677 1106 92-93 -0.062494820631187054 1106 94-95 -0.2419356704022988 1106 96-97 -0.17464873623768895 1106 98-99 -0.4116950392267391 1106 100-101 -0.7515290765105469 1107 1 -0.05059041911600204 1107 2 -0.02342867960719275 1107 3 -0.062200311121955565 1107 4 -0.06709216520712147 1107 5 -0.13418389449198287 1107 6 -0.14189854292779103 1107 7 -0.03733123979093733 1107 8 -0.06774054984392563 1107 9 -0.05537439154928592 1107 10-11 8.452123568787329E-4 1107 12-13 0.07110374434356004 1107 14-15 -0.009772125244481344 1107 16-17 0.04955196942628248 1107 18-19 -0.08262476796369356 1107 20-21 -0.01510358001637968 1107 22-23 0.037649156601126776 1107 24-25 -0.009927347433695388 1107 26-27 -0.08391549868217396 1107 28-29 0.017382631069146726 1107 30-31 -0.0722146673791002 1107 32-33 0.029018177842011994 1107 34-35 -0.042614786618706546 1107 36-37 -0.024496950577571397 1107 38-39 -0.02385691786577837 1107 40-41 0.024415805999815632 1107 42-43 -0.048365810962344824 1107 44-45 -0.08841144994016048 1107 46-47 -0.0011152021204097196 1107 48-49 -0.08669694965512065 1107 50-51 -0.18080972350112035 1107 52-53 -0.09291747539312212 1107 54-55 -0.061683290437578364 1107 56-57 -0.21839643944974085 1107 58-59 -0.18891796500830793 1107 60-61 0.090822857952368 1107 62-63 -0.16764499746327033 1107 64-65 -0.14982327515769356 1107 66-67 -0.18279232684028557 1107 68-69 -0.22763431129645184 1107 70-71 -0.23256431154316104 1107 72-73 -0.272507441744235 1107 74-75 -0.31733907875418765 1107 76-77 -0.39790736224349743 1107 78-79 -0.4309424460801239 1107 80-81 -0.6397421228619393 1107 82-83 -0.6787340473622905 1107 84-85 -0.6788725584958399 1107 86-87 -0.6063869414978917 1107 88-89 -0.6470257586855084 1107 90-91 -0.7092160395507676 1107 92-93 -0.8597265908794398 1107 94-95 -0.7783112908274319 1107 96-97 -0.8228999978252993 1107 98-99 -0.8250940500060331 1107 100-101 -1.0596907566800802 1108 1 -0.017240767767379594 1108 2 -0.021747028678980485 1108 3 -0.04602192254653659 1108 4 0.013997642679910882 1108 5 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50-51 -0.6268076077017994 2316 52-53 -0.6535992781626518 2316 54-55 -0.5658555856729564 2316 56-57 -0.5077608180693076 2316 58-59 -0.5116368688615012 2316 60-61 -0.5324694817690556 2316 62-63 -0.45584009179265195 2316 64-65 -0.24850924171179045 2316 66-67 -0.43553272775713125 2316 68-69 -0.36169796837420876 2316 70-71 -0.4568194416783804 2316 72-73 -0.30369968110082013 2316 74-75 -0.22432243717873135 2316 76-77 -0.38059646180813544 2316 78-79 -0.2972008719796797 2316 80-81 -0.2951493803248795 2316 82-83 -0.3198346020814711 2316 84-85 -0.5211703369065503 2316 86-87 -0.4381908299572892 2316 88-89 -0.5132814720218271 2316 90-91 -0.43035385810741644 2316 92-93 -0.41740165292932474 2316 94-95 -0.4487162087045746 2316 96-97 -0.5248644022142237 2316 98-99 -0.609597040607003 2316 100-101 -0.5203455016313221 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10772.0 3 3059.0 4 2834.0 5 3297.0 6 3796.0 7 4850.0 8 6088.0 9 7711.0 10 9827.0 11 12586.0 12 16262.0 13 20061.0 14 23793.0 15 29465.0 16 34724.0 17 43422.0 18 42062.0 19 35341.0 20 35479.0 21 37163.0 22 39568.0 23 44793.0 24 49276.0 25 54757.0 26 61970.0 27 71709.0 28 82610.0 29 96195.0 30 113228.0 31 135387.0 32 165640.0 33 206892.0 34 269409.0 35 359822.0 36 456355.0 37 475726.0 38 235016.0 39 14424.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0732459815417 18.195927140655556 14.539699474233961 23.19112740356878 2 15.624108820134442 19.413379203162602 40.23112961998959 24.731382356713365 3 19.265833070285797 23.604380534381697 29.67602109011933 27.453765305213174 4 12.827780281057208 16.234116088006445 36.43336639272871 34.50473723820764 5 13.616104817417755 37.06038369755299 34.10485428443734 15.218657200591924 6 32.79737226462666 35.84459879075578 16.63915087568514 14.71887806893242 7 28.877858026945958 33.06705795311149 19.85410139309696 18.200982626845597 8 27.044052113504186 34.24313236400251 19.146315991543812 19.566499530949486 9 26.56990986463993 16.54679018101963 19.90276808082467 36.98053187351576 10-11 25.263133423084973 26.215564552189345 26.60196557896901 21.919336445756667 12-13 26.507743329083254 23.67881307115151 27.107995192306237 22.705448407458995 14-15 22.923676740232356 24.957845176500577 25.745867800419752 26.372610282847315 16-17 22.135988568130568 28.237656762741143 26.817869438640308 22.808485230487985 18-19 22.265732001704553 26.172521611182635 29.596259044864702 21.965487342248107 20-21 22.46956425094934 25.360690486541614 30.26231802011075 21.907427242398292 22-23 22.358143766346032 25.298672735187544 30.192966452947832 22.15021704551859 24-25 22.212286723873778 25.594072109251055 30.09511984508078 22.098521321794387 26-27 21.46177345829442 26.066562075993964 29.88589367031103 22.585770795400588 28-29 21.887261852236726 26.454978759334974 29.722057887795778 21.935701500632522 30-31 21.86719134818928 25.841400276520528 29.603682592838094 22.6877257824521 32-33 21.453630929111803 25.950016073240224 29.685425021316487 22.910927976331493 34-35 22.526175710703527 26.099240978353606 29.38526552474292 21.989317786199948 36-37 22.734994615260963 25.422051091249255 29.315696646367922 22.52725764712186 38-39 21.998283795934373 25.89829491640559 29.22203325971491 22.88138802794513 40-41 22.61541073281147 25.74810902574743 29.34156748090163 22.29491276053947 42-43 22.20324862062872 25.776417432916976 29.154498184706203 22.8658357617481 44-45 22.144122739416513 26.187042067205418 29.384492459139466 22.284342734238603 46-47 22.797990943947628 26.63321056506248 28.289699521274663 22.279098969715232 48-49 22.39365969200399 26.71269200715818 28.131699382447916 22.761948918389912 50-51 22.745427162566084 26.962090200266143 28.06496408530199 22.22751855186578 52-53 23.146608183720446 26.391229006262083 28.164755124536484 22.297407685480984 54-55 22.358060930810428 26.878274481069912 28.341505987428526 22.422158600691134 56-57 22.494871761606834 26.986496569156472 28.053960623214845 22.46467104602185 58-59 22.513680397310186 26.39123899298756 28.009876020126246 23.08520458957601 60-61 21.959622865932864 26.847960851535824 28.432317397537172 22.760098884994147 62-63 22.179867422081955 26.8162619497271 28.59723074750262 22.406639880688324 64-65 21.845481790289604 26.913999039243752 28.336696784846122 22.903822385620522 66-67 21.67929865175008 26.865785133343433 28.433553384746386 23.0213628301601 68-69 22.570472552461773 26.40734966582345 28.553537439408856 22.468640342305925 70-71 22.285750326903504 26.779273970936746 27.835369193483118 23.099606508676636 72-73 22.209774006798387 26.962940229252368 27.880019120458893 22.94726664349035 74-75 22.25642755689319 27.40995475823128 27.812507826708305 22.52110985816722 76-77 22.545046499125405 26.82717156315862 27.860346349548642 22.767435588167327 78-79 22.639724601790522 27.18872473559174 27.73661584281871 22.43493481979902 80-81 22.91637219050889 27.272155405571546 27.315193551730175 22.496278852189384 82-83 22.72950663137656 27.318764347417098 27.467438962101546 22.484290059104797 84-85 22.705556087697293 27.505752787396137 27.402666457497254 22.38602466740932 86-87 23.056582757113233 27.484544710445817 26.882820982929562 22.576051549511384 88-89 22.825672456684277 27.612476015353195 26.760897499258675 22.80095402870386 90-91 22.762544024616496 27.590732339842788 26.855344421243778 22.79137921429694 92-93 22.973478788464206 27.085730586026408 27.10500419582108 22.8357864296883 94-95 22.783833339093505 26.863047858596122 27.259352875365778 23.09376592694459 96-97 23.158498219183024 26.428950752385926 27.280628112807864 23.131922915623186 98-99 22.88463262128738 26.800920570606507 26.73491978656427 23.579527021541846 100-101 23.736728161613023 25.710925682839132 26.97880688639912 23.57353926914872 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1292.0 1 930.5 2 781.5 3 1045.0 4 874.0 5 812.0 6 1587.0 7 3422.5 8 5677.5 9 6537.5 10 5241.0 11 3848.0 12 3681.0 13 3952.0 14 4312.5 15 4589.0 16 4815.0 17 5216.5 18 5758.5 19 6380.0 20 7190.5 21 8387.0 22 10010.5 23 12015.5 24 14577.0 25 18142.5 26 22641.0 27 27612.0 28 32238.5 29 36306.5 30 40000.5 31 46996.5 32 58723.0 33 62256.5 34 59339.5 35 61385.5 36 66887.5 37 72712.5 38 76934.0 39 80155.0 40 84795.5 41 91476.5 42 95299.0 43 98493.5 44 102656.5 45 108804.5 46 112266.5 47 115642.0 48 118236.0 49 114962.0 50 114498.5 51 114430.0 52 113148.0 53 115056.0 54 118725.0 55 114132.5 56 105392.5 57 98491.5 58 92763.0 59 83220.0 60 67023.0 61 55927.0 62 48741.5 63 36728.5 64 26557.5 65 21142.0 66 15859.5 67 11961.0 68 9224.0 69 7002.5 70 5368.5 71 3777.5 72 2557.5 73 1928.0 74 1412.5 75 981.0 76 672.0 77 415.5 78 305.0 79 240.0 80 147.0 81 80.0 82 50.0 83 35.5 84 32.0 85 25.5 86 13.5 87 11.5 88 12.0 89 8.0 90 5.0 91 2.5 92 1.5 93 2.5 94 2.0 95 4.0 96 3.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14390555018159365 2 0.04958724051530916 3 0.14520254004908653 4 0.12538574137599767 5 0.18543938849642377 6 0.19183385016871424 7 0.15871536471505887 8 0.06916273874793424 9 0.11778477750138824 10-11 0.0985109048193429 12-13 0.1460470915907098 14-15 0.13150874005276636 16-17 0.12892984159530962 18-19 0.1277685832255776 20-21 0.12832659049415013 22-23 0.18913430149102559 24-25 0.20528634972457063 26-27 0.1413115704466079 28-29 0.12161542199375093 30-31 0.09463501649439324 32-33 0.11957944952733768 34-35 0.0888890497558492 36-37 0.0834447085678849 38-39 0.05008492267376572 40-41 0.05825897509447667 42-43 0.06012905350807105 44-45 0.038939858579844354 46-47 0.05047703588951938 48-49 0.04215217069351858 50-51 0.0528900402941573 52-53 0.027101055719589585 54-55 0.05055244227716432 56-57 0.026482723340901116 58-59 0.050854067827744064 60-61 0.03667766695049631 62-63 0.05112553082326582 64-65 0.03960343479111978 66-67 0.03906050880007625 68-69 0.0626023830228249 70-71 0.05127634359855569 72-73 0.04949675285013523 74-75 0.04210692686093162 76-77 0.0206010251045962 78-79 0.02263699757100944 80-81 0.026166016512792392 82-83 0.022561591183364507 84-85 0.020254155721429502 86-87 0.024371344486842944 88-89 0.061878481701433535 90-91 0.10386475834213327 92-93 0.07688435284277557 94-95 0.0721035878660867 96-97 0.06462327421170917 98-99 0.05344804756272982 100-101 0.1259437486445702 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3315369.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.42592143637332 #Duplication Level Percentage of deduplicated Percentage of total 1 72.15021617113567 33.49640267578497 2 14.270894704270557 13.250788727344421 3 5.432274315335101 7.5659502175372815 4 2.6695975422897935 4.957541030603251 5 1.4788935930237037 3.4329498881237166 6 0.9220608981705448 2.568451609081052 7 0.5876195258731318 1.9096544559865474 8 0.4304529407978995 1.598733952923131 9 0.3072251294521061 1.2836888750900795 >10 1.574310275979807 12.97933492745199 >50 0.10273551616660054 3.2707500356557206 >100 0.06331137579205545 5.637397852751199 >500 0.005464206150084999 1.7109562321844924 >1k 0.0044234049774207 4.25048229409999 >5k 3.903004391841794E-4 1.1435568304751194 >10k+ 1.3010014639472646E-4 0.9433603949070749 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17484 0.5273621126336164 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13753 0.4148256197123156 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8749 0.263892194202214 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6413 0.19343246558678687 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6228 0.1878523929010617 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5873 0.17714468585548093 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5450 0.16438592506595795 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5153 0.1554276462137397 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4752 0.14333246163549218 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4625 0.13950181714312948 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4339 0.13087532639654892 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3983 0.12013745679591019 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3557 0.10728820834121329 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3411 0.10288447530274909 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3393 0.10234154931170558 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3364 0.10146683521502432 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.016255505797394E-5 3.016255505797394E-5 2 0.0 0.0 0.0 6.032511011594788E-5 3.016255505797394E-5 3 0.0 0.0 0.0 6.032511011594788E-5 3.016255505797394E-5 4 0.0 0.0 0.0 9.048766517392182E-5 3.016255505797394E-5 5 0.0 0.0 0.0 9.048766517392182E-5 3.016255505797394E-5 6 0.0 0.0 0.0 9.048766517392182E-5 9.048766517392182E-5 7 0.0 0.0 0.0 9.048766517392182E-5 9.048766517392182E-5 8 0.0 0.0 0.0 9.048766517392182E-5 9.048766517392182E-5 9 0.0 0.0 0.0 9.048766517392182E-5 1.2065022023189575E-4 10-11 0.0 0.0 0.0 9.048766517392182E-5 1.2065022023189575E-4 12-13 0.0 0.0 0.0 9.048766517392182E-5 1.9605660787683059E-4 14-15 0.0 0.0 0.0 9.048766517392182E-5 2.2621916293480453E-4 16-17 0.0 0.0 0.0 9.048766517392182E-5 2.7146299552176543E-4 18-19 0.0 0.0 0.0 9.048766517392182E-5 3.167068281087264E-4 20-21 0.0 0.0 0.0 1.6589405281885666E-4 4.0719449328264817E-4 22-23 0.0 0.0 0.0 2.2621916293480453E-4 4.9768215845657E-4 24-25 0.0 0.0 0.0 5.730885461015049E-4 6.334136562174528E-4 26-27 0.0 0.0 0.0 0.0012517460349059184 7.088200438623876E-4 28-29 0.0 0.0 0.0 0.004313245373290273 7.239013213913746E-4 30-31 0.0 0.0 0.0 0.013075467617631703 7.239013213913746E-4 32-33 1.508127752898697E-5 0.0 0.0 0.032273933912032114 7.239013213913746E-4 34-35 3.016255505797394E-5 0.0 0.0 0.06178799403625962 7.239013213913746E-4 36-37 3.016255505797394E-5 0.0 0.0 0.10945991230538743 7.540638764493484E-4 38-39 3.016255505797394E-5 0.0 0.0 0.18071894862985086 7.540638764493484E-4 40-41 3.016255505797394E-5 0.0 0.0 0.2568643188737061 7.540638764493484E-4 42-43 3.016255505797394E-5 0.0 0.0 0.33181826819277127 7.540638764493484E-4 44-45 3.016255505797394E-5 0.0 0.0 0.4241910930578165 7.993077090363093E-4 46-47 3.016255505797394E-5 0.0 0.0 0.5304839370821166 8.596328191522572E-4 48-49 3.016255505797394E-5 0.0 0.0 0.6372443007098154 0.001025526871971114 50-51 4.524383258696091E-5 0.0 0.0 0.7587390724833345 0.001055689427029088 52-53 6.032511011594788E-5 0.0 0.0 0.8721351982237875 0.0011612583697319966 54-55 6.032511011594788E-5 0.0 0.0 0.9913376158129004 0.0012517460349059184 56-57 6.032511011594788E-5 0.0 0.0 1.1279438276704643 0.0013120711450218662 58-59 6.032511011594788E-5 0.0 0.0 1.2701904373238695 0.0013422337000798404 60-61 6.032511011594788E-5 0.0 0.0 1.4272468615107399 0.001387477532666801 62-63 6.032511011594788E-5 0.0 0.0 1.5794471143332762 0.0014779651978407231 64-65 6.032511011594788E-5 0.0 0.0 1.7339548026177478 0.00149304647536971 66-67 6.032511011594788E-5 0.0 0.0 1.9152619210712292 0.0015684528630146447 68-69 9.048766517392182E-5 0.0 0.0 2.109795319917632 0.0015986154180726187 70-71 9.048766517392182E-5 0.0 0.0 2.307646599820412 0.0015986154180726187 72-73 9.048766517392182E-5 0.0 0.0 2.521091920688165 0.0016438592506595796 74-75 9.048766517392182E-5 0.0 0.0 2.7275093662274097 0.0017041843607755276 76-77 9.048766517392182E-5 0.0 0.0 2.947726180705677 0.0017645094708914756 78-79 9.048766517392182E-5 0.0 0.0 3.1926008839438387 0.0018097533034784363 80-81 9.048766517392182E-5 0.0 0.0 3.446886304360088 0.0018399158585364103 82-83 9.048766517392182E-5 0.0 0.0 3.7123620327028455 0.001975647356297293 84-85 9.048766517392182E-5 0.0 0.0 3.9837194592819083 0.00199072863382628 86-87 9.048766517392182E-5 0.0 0.0 4.256207981675645 0.0020661350214712148 88-89 9.048766517392182E-5 0.0 0.0 4.545059690188332 0.0021415414091161497 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7465 0.0 56.634552 1 GTATCAA 14125 0.0 43.81807 1 TATCAAC 17985 0.0 34.298965 2 ATCAACG 17925 0.0 34.22804 3 TCAACGC 18195 0.0 33.72012 4 CAACGCA 18330 0.0 33.41988 5 AACGCAG 18895 0.0 32.464516 6 ACGCAGA 21410 0.0 28.509506 7 GTGGTAT 3355 0.0 28.381529 1 CGCAGAG 21470 0.0 28.29613 8 TGGTATC 3130 0.0 28.111166 2 TAAGGTA 1215 0.0 26.227083 4 GCAGAGT 23365 0.0 25.492178 9 GTGTAAG 1470 0.0 24.290823 1 GTACATG 11855 0.0 24.05601 1 TAAGGTG 2480 0.0 23.972303 5 GAGTACT 15310 0.0 23.301157 12-13 TACATGG 11800 0.0 23.175938 2 AGGTAAG 2220 0.0 23.13781 2 AGAGTAC 20725 0.0 22.385006 10-11 >>END_MODULE