FastQCFastQC Report
Thu 26 May 2016
SRR937980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937980_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3315369
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172820.5212692765119056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142040.42842893204346183No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90730.27366486204099755No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA64910.19578514488130883No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA64470.19445799245875797No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA58720.17711452330042296No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA56330.1699056726415672No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC55910.1686388453291323No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG47960.144659614058043No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA46570.14046701890498464No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA43180.13024191274033148No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA41270.12448086472425844No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA35650.1075295087816771No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG34820.10502601671186526No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34320.10351788895896655No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT33880.1021907365364157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78950.057.1618581
GTATCAA136950.040.1681061
ATCAACG175050.031.0941563
TATCAAC175650.031.0732782
TCAACGC178600.030.5260664
CAACGCA180350.030.2825265
AACGCAG185300.029.5504636
TGGTATC32550.028.4576362
TAAGGTG24100.027.982195
GTGGTAT35650.027.4500121
ACGCAGA209700.025.9757927
AGGTAAG23700.025.8557152
CGCAGAG210450.025.7478268
GTAAGGT26450.024.598314
AAGGTAA24550.023.413631
GCAGAGT231750.023.1764379
GAGTACT160050.022.6560112-13
TACATGG111100.022.4043542
GTACATG112000.022.3525681
GTACTTT163950.021.91396714-15
Produced by FastQC (version 0.11.5)