FastQCFastQC Report
Thu 26 May 2016
SRR937979_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937979_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206608
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85730.388514860818052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69230.31373945893425564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41250.18693850470949078No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30100.1364084604061981No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27620.12516949091093663No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27330.12385525657479715No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24020.10885485777265377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47900.044.0293921
GTATCAA80250.039.896371
TATCAAC98400.032.411972
ATCAACG98450.032.3955083
TCAACGC100700.031.6716734
CAACGCA101750.031.2046995
AACGCAG104300.030.4811346
ACGCAGA116650.027.0861597
CGCAGAG117550.026.6766838
TAAGGTG13050.025.1313925
GTACATG72700.024.7435991
GCAGAGT127450.024.2678879
TACATGG70250.023.951622
GAGTACT74650.023.13111112-13
ACATGGG72750.022.2791863
AGAGTAC105600.022.17861610-11
CAGAGTA118500.021.48809210-11
GTACTTT80400.021.47878314-15
AGTACTT77400.021.2659212-13
GTAAGGT13600.020.9696064
Produced by FastQC (version 0.11.5)