FastQCFastQC Report
Thu 26 May 2016
SRR937979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937979_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206608
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83070.3764601596658763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71290.32307505456338415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44220.2003980770485741No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29820.13513954449544277No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28840.13069833880779913No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26730.12113615105175002No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24520.11112077904185973No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23910.10835635509342846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50150.041.7356871
GTATCAA82450.037.3861051
ATCAACG102300.029.9834633
TCAACGC102800.029.9301474
TATCAAC102850.029.9244252
CAACGCA105400.029.1467175
AACGCAG108550.028.3081116
ACGCAGA123250.024.8162067
CGCAGAG124650.024.5374818
GTGTAAG10500.024.4641591
TAAGGTG13300.022.526135
TACATGG70750.022.4566962
GTACATG72450.022.323711
GAGTACT78300.022.13948412-13
GCAGAGT138150.021.8646559
TGTAAGG10300.021.2444952
AGAGTAC111150.021.21515510-11
GTACTTT82600.020.72820314-15
ACATGGG73700.020.6481063
AGTACTT83200.020.29330312-13
Produced by FastQC (version 0.11.5)