##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937979_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2206608 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48257914409809 33.0 31.0 34.0 31.0 34.0 2 32.712675291669385 34.0 31.0 34.0 31.0 34.0 3 32.77957616395844 34.0 31.0 34.0 31.0 34.0 4 36.234423604011226 37.0 35.0 37.0 35.0 37.0 5 36.15918232871448 37.0 35.0 37.0 35.0 37.0 6 36.21192028670249 37.0 37.0 37.0 35.0 37.0 7 36.18239895803876 37.0 37.0 37.0 35.0 37.0 8 36.176618139696764 37.0 37.0 37.0 35.0 37.0 9 37.868785937511326 39.0 38.0 39.0 35.0 39.0 10-11 37.840737910856845 39.0 38.0 39.0 35.0 39.0 12-13 37.757147395459455 39.0 38.0 39.0 35.0 39.0 14-15 39.00884660981923 40.0 38.0 41.0 36.0 41.0 16-17 38.914478466496995 40.0 38.0 41.0 35.0 41.0 18-19 39.16439326785727 40.0 39.0 41.0 36.0 41.0 20-21 39.13254075939179 40.0 39.0 41.0 36.0 41.0 22-23 39.023984096858165 40.0 38.5 41.0 35.0 41.0 24-25 38.90775253239361 40.0 38.0 41.0 35.0 41.0 26-27 38.74245425558142 40.0 38.0 41.0 35.0 41.0 28-29 38.61883646755563 40.0 38.0 41.0 34.5 41.0 30-31 38.384408784886126 40.0 38.0 41.0 34.0 41.0 32-33 38.158462898711505 40.0 38.0 41.0 34.0 41.0 34-35 38.058238935053254 40.0 38.0 41.0 33.0 41.0 36-37 38.00216644732549 40.0 38.0 41.0 33.0 41.0 38-39 37.801932422976805 40.0 37.0 41.0 33.0 41.0 40-41 37.57932514520023 40.0 37.0 41.0 33.0 41.0 42-43 37.315697668095105 40.0 37.0 41.0 32.0 41.0 44-45 37.04310869896239 40.0 36.0 41.0 31.0 41.0 46-47 36.672355488605135 39.0 36.0 41.0 30.5 41.0 48-49 36.246381323733075 39.0 35.0 41.0 30.0 41.0 50-51 35.849860736478796 39.0 35.0 40.0 28.5 41.0 52-53 35.29361989080073 38.0 34.5 40.0 27.0 41.0 54-55 34.889629240898245 38.0 34.0 40.0 26.0 41.0 56-57 34.486411043556444 38.0 34.0 40.0 26.0 41.0 58-59 33.94763342650802 37.0 33.0 40.0 23.5 41.0 60-61 33.43195868953616 36.5 32.5 39.5 22.5 41.0 62-63 32.84525933015742 36.0 32.0 39.0 21.0 40.0 64-65 32.2394163349358 35.0 31.0 39.0 18.5 40.0 66-67 31.643009315655522 35.0 31.0 38.0 15.0 40.0 68-69 30.98272574920421 35.0 30.0 37.5 8.0 40.0 70-71 30.33631732505275 34.0 29.0 37.0 7.0 39.0 72-73 29.646318693669198 34.0 28.5 36.0 2.0 39.0 74-75 28.973568481578965 34.0 27.5 36.0 2.0 38.5 76-77 27.68750294569765 31.5 26.0 34.5 2.0 36.5 78-79 28.326446518819836 33.0 27.0 35.0 2.0 37.0 80-81 28.045205582504913 33.0 26.5 35.0 2.0 36.5 82-83 27.474807713921095 33.0 26.0 35.0 2.0 36.0 84-85 26.803748558874073 33.0 25.0 35.0 2.0 36.0 86-87 26.077237778527042 32.0 23.5 35.0 2.0 35.0 88-89 25.24892640650265 31.5 20.0 34.0 2.0 35.0 90-91 24.56410540521923 31.0 17.0 34.0 2.0 35.0 92-93 23.602874864951094 31.0 4.5 34.0 2.0 35.0 94-95 22.673921013610027 30.0 2.0 34.0 2.0 35.0 96-97 21.59402168396018 29.5 2.0 34.0 2.0 35.0 98-99 20.156385275499773 29.0 2.0 34.0 2.0 35.0 100-101 16.349370617708264 13.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14400136267106234 1101 2 0.11866943150299392 1101 3 0.12012471385288848 1101 4 -0.038990413677865376 1101 5 -0.03569824619012252 1101 6 0.03828510025925169 1101 7 0.04661225103410516 1101 8 0.01253570655937608 1101 9 0.01995667816095903 1101 10-11 0.1328097513711768 1101 12-13 0.17912021997899075 1101 14-15 0.25207052041147904 1101 16-17 0.2639283770606866 1101 18-19 0.16625042393412315 1101 20-21 0.09820763000319488 1101 22-23 0.24840193348398998 1101 24-25 0.2344206453064146 1101 26-27 0.23471198048683561 1101 28-29 0.24390005821102534 1101 30-31 0.37820034975502637 1101 32-33 0.3355072930290319 1101 34-35 0.34516641564336226 1101 36-37 0.41463336003724294 1101 38-39 0.4315500437263964 1101 40-41 0.5654115098553589 1101 42-43 0.7240554446948124 1101 44-45 0.6907026065750586 1101 46-47 0.745920391511639 1101 48-49 0.9661319585397976 1101 50-51 0.9194909583303854 1101 52-53 0.9753491668983401 1101 54-55 1.0983684728448821 1101 56-57 1.2607553115564087 1101 58-59 1.5305560793037642 1101 60-61 1.3593801275087003 1101 62-63 1.3530516854102572 1101 64-65 1.4564336055982423 1101 66-67 1.6544032478945674 1101 68-69 1.810218092366668 1101 70-71 1.8933126626578378 1101 72-73 2.180343304229055 1101 74-75 2.294675736190449 1101 76-77 2.4030904355960594 1101 78-79 2.5237145403994 1101 80-81 2.5549129329210345 1101 82-83 2.71415876824182 1101 84-85 2.8736641730058388 1101 86-87 3.1131736443248315 1101 88-89 3.3994589417869427 1101 90-91 3.7653758198463514 1101 92-93 3.7887139728939125 1101 94-95 4.156836317487532 1101 96-97 4.323023573662091 1101 98-99 4.760849949571522 1101 100-101 4.800509948339336 1102 1 0.18385611349850706 1102 2 0.18507753123383708 1102 3 0.23916350754787885 1102 4 0.14571861197318015 1102 5 0.03542513524226365 1102 6 0.07840295139244802 1102 7 0.11411107957010813 1102 8 0.20271914731296903 1102 9 0.16732054415289355 1102 10-11 0.21318491658160355 1102 12-13 0.338083248698716 1102 14-15 0.4311435369281966 1102 16-17 0.3550139996428001 1102 18-19 0.430946146205045 1102 20-21 0.3223035627049029 1102 22-23 0.4960611691330925 1102 24-25 0.4706645669846097 1102 26-27 0.3079086368380217 1102 28-29 0.30634644596005245 1102 30-31 0.4742470451138061 1102 32-33 0.45243114601431955 1102 34-35 0.4712961829454798 1102 36-37 0.4883909590985951 1102 38-39 0.5550563498500267 1102 40-41 0.6598755233466633 1102 42-43 0.7437608910442322 1102 44-45 0.6641476503859565 1102 46-47 0.8230965636247376 1102 48-49 0.8933664256689582 1102 50-51 1.0223940872211017 1102 52-53 1.039085215967532 1102 54-55 1.0671651227669372 1102 56-57 1.1765897957475175 1102 58-59 1.2721745170962464 1102 60-61 1.2890299741365538 1102 62-63 1.4228171576987947 1102 64-65 1.3924793365855948 1102 66-67 1.4998941314937078 1102 68-69 1.7733066525725008 1102 70-71 2.0898270781560306 1102 72-73 2.2428830381861786 1102 74-75 2.326590866559865 1102 76-77 2.2806197160142503 1102 78-79 2.190185324272637 1102 80-81 2.3728752161074667 1102 82-83 2.5333429594742007 1102 84-85 2.794865878706883 1102 86-87 2.9604928772291856 1102 88-89 3.2085550661265607 1102 90-91 3.432778705789648 1102 92-93 3.7220526638462523 1102 94-95 3.8786049004920926 1102 96-97 4.117258734396845 1102 98-99 4.4643474032600565 1102 100-101 4.306450847207682 1103 1 0.2614957945303473 1103 2 0.17675656100747972 1103 3 0.1956739133731773 1103 4 0.10770915419634264 1103 5 0.07512726636436895 1103 6 0.07341197772208119 1103 7 0.09973754847752048 1103 8 0.13887158442197034 1103 9 0.22670745389338265 1103 10-11 0.20503543715671668 1103 12-13 0.21860761789641714 1103 14-15 0.3803631000311256 1103 16-17 0.38974313406131245 1103 18-19 0.2723296914142779 1103 20-21 0.25148117324432206 1103 22-23 0.40783695756500293 1103 24-25 0.3965693051453627 1103 26-27 0.4329676533276299 1103 28-29 0.4450111878297349 1103 30-31 0.502328959528306 1103 32-33 0.5150167731455397 1103 34-35 0.543092640158406 1103 36-37 0.6084941279647538 1103 38-39 0.5339360158190587 1103 40-41 0.660874545967367 1103 42-43 0.666699193148105 1103 44-45 0.8580617611694805 1103 46-47 0.9398793157658858 1103 48-49 0.9534929929766065 1103 50-51 1.0406169363243336 1103 52-53 1.228631633441907 1103 54-55 1.218586296606091 1103 56-57 1.3527230306175397 1103 58-59 1.3949739944395958 1103 60-61 1.419472382558105 1103 62-63 1.5610169820922195 1103 64-65 1.582661570990112 1103 66-67 1.7084870269964334 1103 68-69 1.719013256174712 1103 70-71 1.813662194362923 1103 72-73 1.9259293629372465 1103 74-75 1.9715772324497998 1103 76-77 2.0141299884315274 1103 78-79 2.129360730268239 1103 80-81 2.2784944847666146 1103 82-83 2.3934407636350166 1103 84-85 2.564178226612281 1103 86-87 2.885672978051584 1103 88-89 2.957532507871729 1103 90-91 3.1278836723524925 1103 92-93 3.0934418954539247 1103 94-95 3.1122258731784562 1103 96-97 3.5909935580522614 1103 98-99 3.733191995220757 1103 100-101 3.5273680178601907 1104 1 0.1871597067350379 1104 2 0.09252332214386172 1104 3 0.1426681655602735 1104 4 0.042013335838817056 1104 5 -0.0060076552185250875 1104 6 0.022773854665317117 1104 7 0.049579465525141586 1104 8 0.09703072789650236 1104 9 0.0748098250471827 1104 10-11 0.1622130094658658 1104 12-13 0.14471831577790084 1104 14-15 0.2206886857615018 1104 16-17 0.25929936164285294 1104 18-19 0.18208305667186409 1104 20-21 0.2760231670259756 1104 22-23 0.2898505878083526 1104 24-25 0.2551419572470408 1104 26-27 0.28885755538316715 1104 28-29 0.27524405478668257 1104 30-31 0.3073738597921718 1104 32-33 0.3566422224196444 1104 34-35 0.3550442814340755 1104 36-37 0.3902925997993876 1104 38-39 0.46212025168208726 1104 40-41 0.5919456959642915 1104 42-43 0.6697474025668484 1104 44-45 0.7224562003052881 1104 46-47 0.8038898585505407 1104 48-49 0.8945387745959152 1104 50-51 1.1962328399941669 1104 52-53 1.1335514563383242 1104 54-55 1.1951179457723384 1104 56-57 1.1091340091935393 1104 58-59 1.289044164448896 1104 60-61 1.3175273152529599 1104 62-63 1.483625558929397 1104 64-65 1.485532740170541 1104 66-67 1.7875995364524506 1104 68-69 1.7825257064514233 1104 70-71 1.723423138010471 1104 72-73 1.984548887946385 1104 74-75 2.055177279865635 1104 76-77 2.2281877208324694 1104 78-79 2.145977990356773 1104 80-81 2.2544669166199185 1104 82-83 2.4208376613362503 1104 84-85 2.6385859132261764 1104 86-87 2.927084694106732 1104 88-89 3.2162155330021243 1104 90-91 3.4200213302997113 1104 92-93 3.658027649347634 1104 94-95 3.923311428902924 1104 96-97 4.078244104601222 1104 98-99 4.510306795838975 1104 100-101 4.129030988082324 1105 1 0.10876095130278429 1105 2 0.04935923591757785 1105 3 0.10353899724450599 1105 4 0.07799581679736889 1105 5 -0.01194312526882868 1105 6 -0.07715861368820498 1105 7 -0.022789771915860513 1105 8 0.044399802059835736 1105 9 0.10892571562240505 1105 10-11 0.04795325166544018 1105 12-13 0.21151684549509042 1105 14-15 0.21845472060886806 1105 16-17 0.17147001424412878 1105 18-19 0.15879672118456511 1105 20-21 0.1488373687875466 1105 22-23 0.2550388077812542 1105 24-25 0.23750796832118937 1105 26-27 0.17593536829829048 1105 28-29 0.1947573044434705 1105 30-31 0.17563032852710592 1105 32-33 0.32982647670437615 1105 34-35 0.26092809042793164 1105 36-37 0.2881813541474685 1105 38-39 0.2850247133425299 1105 40-41 0.25487689597682817 1105 42-43 0.1931225611094689 1105 44-45 0.3416969068665665 1105 46-47 0.28773745124980366 1105 48-49 0.36975616249272747 1105 50-51 0.43227488464865615 1105 52-53 0.4206399802689518 1105 54-55 0.5606760876462715 1105 56-57 0.665932107857671 1105 58-59 0.7556509408642853 1105 60-61 0.9130205901720672 1105 62-63 0.9510212930715412 1105 64-65 1.0409237118294499 1105 66-67 1.2292209098920104 1105 68-69 1.4462406393051097 1105 70-71 1.418601025772091 1105 72-73 1.5182173976914477 1105 74-75 1.4732416034323528 1105 76-77 1.5319909803773548 1105 78-79 1.6479294851312325 1105 80-81 1.9546518775704342 1105 82-83 1.9885867936786887 1105 84-85 2.2997831043276733 1105 86-87 2.481302678946726 1105 88-89 2.497534803555844 1105 90-91 2.7309493744813444 1105 92-93 3.0337834617063173 1105 94-95 3.0559467828301443 1105 96-97 3.5689950774096104 1105 98-99 3.506243467612965 1105 100-101 3.0367941231181845 1106 1 0.11556565845334177 1106 2 0.10956854242134995 1106 3 0.07466702654941315 1106 4 0.1202900536321252 1106 5 -0.020807709849812284 1106 6 -0.0016343493052417557 1106 7 0.1030910720973921 1106 8 0.08019875003763133 1106 9 0.13945706668241087 1106 10-11 0.09498108933063065 1106 12-13 0.11282987699586755 1106 14-15 -0.0065298084764506825 1106 16-17 0.0913400336077217 1106 18-19 -0.013100112767595817 1106 20-21 -0.025761884616052555 1106 22-23 0.001697589272055211 1106 24-25 -0.046949751166181386 1106 26-27 -0.17546524854204648 1106 28-29 0.06120213815609077 1106 30-31 0.10515594536744999 1106 32-33 0.13768832143627918 1106 34-35 0.18383864358798974 1106 36-37 0.2919024834244368 1106 38-39 0.26721507047112425 1106 40-41 0.27453306737459826 1106 42-43 0.38132124757890296 1106 44-45 0.4817860392975959 1106 46-47 0.6241986541882838 1106 48-49 0.6362738592228752 1106 50-51 0.8093154234357343 1106 52-53 0.9245033421486184 1106 54-55 0.8798734965175896 1106 56-57 0.9671957322438232 1106 58-59 0.9353874112998639 1106 60-61 0.9343748192214534 1106 62-63 0.8765662685177062 1106 64-65 0.9425205847084399 1106 66-67 1.2383437589358088 1106 68-69 1.0770347463838021 1106 70-71 0.9958950879352244 1106 72-73 1.4485708332326297 1106 74-75 1.6473018701309599 1106 76-77 1.6226043771913439 1106 78-79 1.854542926860784 1106 80-81 1.6335842347389544 1106 82-83 2.091735998174233 1106 84-85 2.345016814951702 1106 86-87 2.442608548911192 1106 88-89 2.5397335948282524 1106 90-91 2.413654014757622 1106 92-93 2.230118726408673 1106 94-95 2.5859715861998076 1106 96-97 3.013878072379068 1106 98-99 3.2695016671977477 1106 100-101 3.271675670808527 1107 1 0.19873834098000742 1107 2 0.2519906096896065 1107 3 0.22746132515482742 1107 4 0.08534850318093135 1107 5 0.02241590240066671 1107 6 0.09321969772784655 1107 7 0.11012210846600112 1107 8 0.14932815938956168 1107 9 0.2250583108716384 1107 10-11 0.2323939277227609 1107 12-13 0.2645081652102519 1107 14-15 0.39725061263375494 1107 16-17 0.41824306204633643 1107 18-19 0.3481488618017181 1107 20-21 0.2643700541315539 1107 22-23 0.3416722953152558 1107 24-25 0.41148270713297563 1107 26-27 0.3360417927486381 1107 28-29 0.472644235503644 1107 30-31 0.4664673003031865 1107 32-33 0.48641422381527377 1107 34-35 0.5016102118133148 1107 36-37 0.6008929469191031 1107 38-39 0.6607129854287379 1107 40-41 0.5975616768905994 1107 42-43 0.541165382115409 1107 44-45 0.6745054814632994 1107 46-47 0.7350084026159536 1107 48-49 0.8083360003221358 1107 50-51 0.8742038223966375 1107 52-53 0.9708639177570433 1107 54-55 1.1182280220849918 1107 56-57 1.1679442966408686 1107 58-59 1.235277177501672 1107 60-61 1.3344559991503573 1107 62-63 1.345423938739195 1107 64-65 1.493029027421045 1107 66-67 1.7433512787608016 1107 68-69 2.001460505202509 1107 70-71 1.8769408563246124 1107 72-73 2.153708240943871 1107 74-75 2.1215772324497983 1107 76-77 2.0078979921763036 1107 78-79 2.1253548970549296 1107 80-81 2.339246321148579 1107 82-83 2.4949908860604815 1107 84-85 2.351232633929005 1107 86-87 2.69630779009249 1107 88-89 2.6858343946641803 1107 90-91 2.4684983201992416 1107 92-93 2.905599824303124 1107 94-95 3.0257565875670487 1107 96-97 3.4188300480421816 1107 98-99 3.8707262023358346 1107 100-101 3.6710099953915183 1108 1 0.20445292325614872 1108 2 0.19910160290822176 1108 3 0.09649186443608926 1108 4 0.1007945981202667 1108 5 -0.04603914211656246 1108 6 0.07922675590010186 1108 7 0.06689772317282916 1108 8 0.17586088993345328 1108 9 0.10265092055009717 1108 10-11 0.1420464396017067 1108 12-13 0.23466778139330557 1108 14-15 0.26330161138819363 1108 16-17 0.1753704320698617 1108 18-19 0.24977327938717764 1108 20-21 0.3321953989670945 1108 22-23 0.24861167807117823 1108 24-25 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2316 62-63 0.47183855661608476 2316 64-65 0.8310798183491954 2316 66-67 1.1425817914347114 2316 68-69 1.359454597064527 2316 70-71 1.3922924858271877 2316 72-73 1.5291999504921847 2316 74-75 1.889632788005354 2316 76-77 1.8725511273011364 2316 78-79 1.9260929378401421 2316 80-81 2.083063264163634 2316 82-83 2.2212773354601367 2316 84-85 2.3353478426196865 2316 86-87 2.387253092169864 2316 88-89 2.6922547300939534 2316 90-91 3.049122009926709 2316 92-93 3.2753720751131112 2316 94-95 3.681226856292035 2316 96-97 4.062282497060664 2316 98-99 4.243865138871001 2316 100-101 4.478520477571809 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 18.0 5 45.0 6 73.0 7 229.0 8 505.0 9 1060.0 10 1867.0 11 2998.0 12 4643.0 13 7087.0 14 9511.0 15 13085.0 16 16915.0 17 23369.0 18 24698.0 19 21410.0 20 20961.0 21 22201.0 22 24551.0 23 27155.0 24 30315.0 25 34002.0 26 38323.0 27 44652.0 28 53137.0 29 63248.0 30 75690.0 31 90108.0 32 108806.0 33 134849.0 34 173348.0 35 235294.0 36 322495.0 37 382810.0 38 189430.0 39 7709.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52843821829705 18.248687578400876 14.691825643702913 23.531048559599167 2 15.97386557040455 19.412541897352337 39.60558132788828 25.00801120435483 3 19.907085343077696 22.743379796704435 29.284522698649152 28.06501216156872 4 13.238425809480692 16.197000864237644 35.75229235976759 34.812280966514074 5 14.075623770426349 36.827999433760915 33.971123621607106 15.125253174205632 6 33.73571563231892 35.30531929549789 16.40486212322261 14.554102948960576 7 29.772891243030024 32.15455577066702 19.387086424049947 18.685466562253016 8 27.107120861451616 34.7287525021515 18.708910219835847 19.455216416561036 9 26.222237932609687 16.711577226222328 19.511304228027814 37.55488061314017 10-11 25.65879394980894 26.064937678101412 26.363472805319294 21.912795566770356 12-13 26.510100570649612 23.198320680428967 26.95351870382052 23.338060045100896 14-15 22.973065447057202 24.696275913075635 25.311314923176205 27.01934371669096 16-17 22.70149478294287 27.914269321963847 26.248726552246705 23.13550934284658 18-19 22.74760628077121 26.283961627982862 28.519904758797214 22.448527332448716 20-21 23.050650591314813 25.41575123447391 29.07684554755534 22.456752626655934 22-23 22.853079078148212 25.337237979481575 28.935462761295422 22.874220181074794 24-25 22.780361443271495 25.58728413603203 28.893096975525218 22.739257445171255 26-27 22.2458622359505 26.023742389163168 28.74826844893587 22.982126925950457 28-29 22.47177568467077 26.321575921051675 28.632951570917896 22.573696823359654 30-31 22.514556278233382 25.89798459898632 28.485576051568746 23.10188307121156 32-33 22.242532429865207 25.85289276572912 28.600684852044406 23.30388995236127 34-35 23.06839355083456 25.955588450313932 28.37871646687331 22.597301531978193 36-37 23.10369776358595 25.48393097858438 28.406244837907167 23.006126419922502 38-39 22.59932788114872 25.991171358581976 28.29024385269584 23.119256907573458 40-41 23.097703726852068 25.744845798286285 28.313896136181466 22.84355433868018 42-43 22.7391458538459 25.70362875585352 28.274157053849525 23.283068336451056 44-45 22.69747262772482 26.04498212344229 28.36530900906903 22.892236239763864 46-47 23.15724477506761 26.343765259567004 27.624872117039388 22.874117848325994 48-49 22.936674751473753 26.330571628490425 27.621172405792056 23.111581214243763 50-51 22.986887566799357 26.631123425637902 27.543451306258294 22.838537701304446 52-53 23.41115866524548 26.2555922936924 27.459952107488057 22.873296933574064 54-55 22.902481999521438 26.50368801345776 27.639390412796473 22.95443957422433 56-57 22.918638018170874 26.601802404414375 27.474657936525197 23.004901640889546 58-59 22.96733266624611 26.21843118487742 27.380939432830843 23.433296716045625 60-61 22.56948220979893 26.535501548077413 27.664542138884656 23.230474103239 62-63 22.785288551478107 26.534209972953963 27.74958216411796 22.93091931144997 64-65 22.463822301015856 26.620700187799557 27.59284838992698 23.322629121257606 66-67 22.430128051742766 26.600352214802086 27.556185783791232 23.41333394966392 68-69 22.943354310700542 26.189910243636717 27.67251831960208 23.19421712606066 70-71 22.76099787547222 26.35227915424942 27.247703262201533 23.63901970807683 72-73 22.858618943332694 26.461140007908067 27.2384871346626 23.441753914096637 74-75 22.666667573035173 26.927279335523117 27.074768150935736 23.331284940505974 76-77 22.852427798684676 26.354930282134386 27.42290429473654 23.369737624444394 78-79 22.816376991291612 26.660081899458355 27.409036856568996 23.114504252681037 80-81 23.019515038026725 26.620950908318026 27.105790521733375 23.253743531921874 82-83 22.87236501374539 26.726308576525064 27.251538368807893 23.149788040921653 84-85 22.87900157171422 26.814473366967174 27.15743609359223 23.14908896772638 86-87 23.010112898551284 26.751425366020126 26.898239029139987 23.340222706288603 88-89 22.839013544770978 26.98651051750016 26.671910008483607 23.502565929245247 90-91 22.809050174866297 26.818829272758336 26.925444009022257 23.44667654335311 92-93 22.915380283987975 26.52512963904999 26.986222062600625 23.57326801436141 94-95 22.70048026212183 26.38668181813032 27.115198330468832 23.79763958927902 96-97 22.94103904890976 26.122828082694475 27.068217254156785 23.86791561423898 98-99 22.622618063561813 26.35207522133519 26.745167243117034 24.28013947198596 100-101 23.000755004966898 25.62965420228695 27.068763459572338 24.300827333173814 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 485.0 1 338.5 2 293.0 3 422.0 4 379.0 5 354.0 6 692.0 7 1560.0 8 2494.0 9 2852.5 10 2451.0 11 1921.5 12 1795.0 13 1914.5 14 2131.0 15 2267.0 16 2408.5 17 2593.0 18 2792.0 19 3098.5 20 3536.5 21 4151.5 22 5074.0 23 6211.5 24 7571.5 25 9543.5 26 12183.0 27 15290.5 28 17948.0 29 20219.0 30 23065.0 31 27418.5 32 33407.5 33 35655.5 34 35898.5 35 38258.0 36 41736.5 37 45651.0 38 48337.0 39 51255.0 40 55279.5 41 59853.0 42 63229.5 43 66036.0 44 68879.0 45 72456.0 46 75023.0 47 78581.5 48 82387.0 49 82129.5 50 81553.0 51 80813.5 52 79941.5 53 81576.0 54 84170.0 55 81416.5 56 76086.0 57 71728.0 58 67524.5 59 60387.0 60 49075.5 61 40305.0 62 33704.0 63 25437.5 64 18279.0 65 13977.5 66 10472.5 67 7871.0 68 6096.0 69 4711.0 70 3612.0 71 2482.0 72 1763.5 73 1357.5 74 966.0 75 696.5 76 516.5 77 319.0 78 199.0 79 133.5 80 79.0 81 39.5 82 19.5 83 13.5 84 8.0 85 6.5 86 3.5 87 1.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014637851399070428 3 0.0030363345007359713 4 0.00176741858998064 5 0.11746535859563638 6 0.0 7 0.0 8 3.172289776888328E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.7191055230471384E-4 24-25 4.5318425384118974E-4 26-27 0.006004691363395764 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011103014219109149 36-37 7.93072444222082E-4 38-39 0.006933719083770204 40-41 0.010355260200271185 42-43 0.006140646639548121 44-45 0.003942703008418351 46-47 2.265921269205949E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0010649829965267959 70-71 0.0 72-73 2.265921269205949E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.063685076823795E-5 82-83 0.0022659212692059485 84-85 0.004531842538411897 86-87 0.0031496305641962683 88-89 0.0 90-91 0.0029456976499677334 92-93 2.265921269205949E-5 94-95 2.265921269205949E-5 96-97 1.586144888444164E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2206608.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.58624596080498 #Duplication Level Percentage of deduplicated Percentage of total 1 75.89991820656432 40.671916854219354 2 13.541741413988232 14.513021722951846 3 4.570506137681133 7.347487981774499 4 1.9809119760417861 4.245985454995174 5 1.0500047380668391 2.8132906077030126 6 0.6314367520522921 2.030179506249957 7 0.44096828351734657 1.6540884431032161 8 0.28531028199476516 1.223096555689449 9 0.2183938945096111 1.0532618052777105 >10 1.2389979333064876 12.12973511241228 >50 0.08991751198788445 3.322676720697012 >100 0.045716832507178194 4.546738071913664 >500 0.0029611140050748042 1.067111931178896 >1k 0.00304571726236259 2.6816072193572373 >5k 1.6920651457569947E-4 0.6998020124767256 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8307 0.3764601596658763 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7129 0.32307505456338415 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4422 0.2003980770485741 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2982 0.13513954449544277 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2884 0.13069833880779913 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2673 0.12113615105175002 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2452 0.11112077904185973 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2391 0.10835635509342846 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.492513396126543E-4 14-15 0.0 0.0 0.0 2.2659212692059485E-5 2.9456976499677334E-4 16-17 0.0 0.0 0.0 4.531842538411897E-5 3.6254740307295176E-4 18-19 0.0 0.0 0.0 4.531842538411897E-5 3.6254740307295176E-4 20-21 0.0 0.0 0.0 4.531842538411897E-5 3.6254740307295176E-4 22-23 0.0 0.0 0.0 6.797763807617846E-5 3.6254740307295176E-4 24-25 0.0 0.0 0.0 2.0393291422853536E-4 3.6254740307295176E-4 26-27 0.0 0.0 0.0 6.117987426856061E-4 3.8520661576501127E-4 28-29 0.0 0.0 0.0 0.0032855858403486255 4.078658284570708E-4 30-31 0.0 0.0 0.0 0.010355260200271185 4.078658284570708E-4 32-33 0.0 0.0 0.0 0.026828507827398432 4.078658284570708E-4 34-35 0.0 0.0 0.0 0.052184166829813 4.078658284570708E-4 36-37 0.0 0.0 0.0 0.09201906274245357 4.078658284570708E-4 38-39 0.0 0.0 0.0 0.1539693502425442 4.078658284570708E-4 40-41 0.0 0.0 0.0 0.218638743265682 4.078658284570708E-4 42-43 0.0 0.0 0.0 0.2840558903076577 4.5318425384118974E-4 44-45 0.0 0.0 0.0 0.3651758717452307 4.7584346653324925E-4 46-47 0.0 0.0 0.0 0.45411328156156416 5.211618919173682E-4 48-49 0.0 0.0 0.0 0.5469707351736239 5.438211046094277E-4 50-51 0.0 0.0 0.0 0.6473737066121394 5.438211046094277E-4 52-53 0.0 0.0 0.0 0.7421118748776403 5.438211046094277E-4 54-55 0.0 0.0 0.0 0.84333057797307 5.438211046094277E-4 56-57 0.0 0.0 0.0 0.959141814042186 5.664803173014872E-4 58-59 0.0 0.0 0.0 1.0850590589719604 5.891395299935467E-4 60-61 0.0 0.0 0.0 1.2172075873920516 5.891395299935467E-4 62-63 0.0 0.0 0.0 1.3474074235206253 5.891395299935467E-4 64-65 0.0 0.0 0.0 1.4813686889560809 6.344579553776656E-4 66-67 0.0 0.0 0.0 1.6377399157439836 6.344579553776656E-4 68-69 0.0 0.0 0.0 1.811264166539775 6.344579553776656E-4 70-71 0.0 0.0 0.0 1.989161645385134 6.344579553776656E-4 72-73 0.0 0.0 0.0 2.168599950693553 6.57117168069725E-4 74-75 0.0 0.0 0.0 2.345251172840849 7.024355934538441E-4 76-77 0.0 0.0 0.0 2.5428395075156076 7.250948061459035E-4 78-79 0.0 0.0 0.0 2.762815144330121 7.250948061459035E-4 80-81 0.0 0.0 0.0 2.9945509125318135 7.250948061459035E-4 82-83 0.0 0.0 0.0 3.236143438254552 7.250948061459035E-4 84-85 0.0 0.0 0.0 3.475447383495392 7.250948061459035E-4 86-87 0.0 0.0 0.0 3.7231578966449863 7.704132315300225E-4 88-89 0.0 0.0 0.0 3.9861407191490286 8.157316569141416E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5015 0.0 41.735687 1 GTATCAA 8245 0.0 37.386105 1 ATCAACG 10230 0.0 29.983463 3 TCAACGC 10280 0.0 29.930147 4 TATCAAC 10285 0.0 29.924425 2 CAACGCA 10540 0.0 29.146717 5 AACGCAG 10855 0.0 28.308111 6 ACGCAGA 12325 0.0 24.816206 7 CGCAGAG 12465 0.0 24.537481 8 GTGTAAG 1050 0.0 24.464159 1 TAAGGTG 1330 0.0 22.52613 5 TACATGG 7075 0.0 22.456696 2 GTACATG 7245 0.0 22.32371 1 GAGTACT 7830 0.0 22.139484 12-13 GCAGAGT 13815 0.0 21.864655 9 TGTAAGG 1030 0.0 21.244495 2 AGAGTAC 11115 0.0 21.215155 10-11 GTACTTT 8260 0.0 20.728203 14-15 ACATGGG 7370 0.0 20.648106 3 AGTACTT 8320 0.0 20.293303 12-13 >>END_MODULE