FastQCFastQC Report
Thu 26 May 2016
SRR937978_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937978_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162255
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83030.3839972621175578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68940.31883381007328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42280.19553660414705942No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28250.1306506401881369No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27430.12685830302161402No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27170.1256558546517409No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC22540.1042430240651542No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22070.10206936739653742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50350.044.8485341
GTATCAA80600.036.0718881
TATCAAC102500.028.333442
TCAACGC101900.028.3143424
ATCAACG102000.028.2865853
CAACGCA104200.027.643755
AACGCAG107650.026.7970036
GTACATG71250.024.18851
ACGCAGA119850.023.8610237
TACATGG70200.023.4272292
CGCAGAG122200.023.4021598
ACATGGG71050.022.2777943
GAGTACT76550.021.96841612-13
TAAGGTG13000.021.9381455
TCGCGTA1100.002876753521.592759
GTAAGGT12600.021.5028634
GCAGAGT134400.020.9950949
AGGTAAG12250.020.9526822
AGAGTAC110250.020.76748810-11
GTGTAAG9200.020.2378041
Produced by FastQC (version 0.11.5)