FastQCFastQC Report
Thu 26 May 2016
SRR937978_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937978_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162255
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82440.38126862927823035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70680.3268809645485847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43140.19951393337048592No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29210.1350904495538223No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26600.12301971784086521No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26450.12232599762747687No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24360.11266016265426602No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23600.10914531357309847No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG21930.10142189519737498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54750.039.481221
GTATCAA86300.035.3967551
TATCAAC105300.029.0098762
ATCAACG105300.028.960073
TCAACGC106650.028.7251114
CAACGCA109100.027.9929755
AACGCAG111850.027.3047266
ACGCAGA124500.024.3396427
CGCAGAG126700.023.9544988
GAGTACT77350.023.11891712-13
TACATGG70500.022.4397722
GTACATG73900.022.3716531
GTACTTT81700.021.71357714-15
ACATGGG72100.021.279443
GCAGAGT141200.021.2591179
TAAGGTG11450.021.1557085
GTATAGG4500.021.11451
GTGTAGC13600.020.9592461
AGAGTAC112500.020.8774110-11
TAAGGTA7100.020.0694
Produced by FastQC (version 0.11.5)