##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937978_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2162255 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49644792126738 33.0 31.0 34.0 31.0 34.0 2 32.727566822599556 34.0 31.0 34.0 31.0 34.0 3 32.694259002754066 34.0 31.0 34.0 31.0 34.0 4 36.215962964590204 37.0 35.0 37.0 35.0 37.0 5 36.17670857507556 37.0 35.0 37.0 35.0 37.0 6 36.23718941567947 37.0 37.0 37.0 35.0 37.0 7 36.19743786001189 37.0 37.0 37.0 35.0 37.0 8 36.18991007073634 37.0 37.0 37.0 35.0 37.0 9 37.90030176829283 39.0 38.0 39.0 35.0 39.0 10-11 37.86501222103776 39.0 38.0 39.0 35.0 39.0 12-13 37.80596923119614 39.0 38.0 39.0 35.0 39.0 14-15 39.09628582197752 40.0 38.5 41.0 36.0 41.0 16-17 39.02561099407794 40.0 38.0 41.0 36.0 41.0 18-19 39.01531271751019 40.0 38.5 41.0 36.0 41.0 20-21 39.01215328441835 40.0 38.5 41.0 35.5 41.0 22-23 39.041985334754685 40.0 39.0 41.0 35.5 41.0 24-25 38.98716640729239 40.0 38.5 41.0 35.0 41.0 26-27 38.81275890216464 40.0 38.0 41.0 35.0 41.0 28-29 38.73767525106891 40.0 38.0 41.0 35.0 41.0 30-31 38.530060515526614 40.0 38.0 41.0 34.0 41.0 32-33 38.33225267140092 40.0 38.0 41.0 34.0 41.0 34-35 38.128582428991955 40.0 38.0 41.0 34.0 41.0 36-37 38.043448621924796 40.0 38.0 41.0 33.0 41.0 38-39 37.99482785332904 40.0 38.0 41.0 33.0 41.0 40-41 37.87599543069619 40.0 37.5 41.0 33.0 41.0 42-43 37.68093286869495 40.0 37.0 41.0 33.0 41.0 44-45 37.43508674971268 40.0 37.0 41.0 32.5 41.0 46-47 37.09131069184717 40.0 36.5 41.0 31.5 41.0 48-49 36.71930068377689 40.0 36.0 41.0 31.0 41.0 50-51 36.407952346046144 39.0 35.5 41.0 30.0 41.0 52-53 35.92998744366413 39.0 35.0 40.0 29.0 41.0 54-55 35.60136616634023 38.5 35.0 40.0 28.0 41.0 56-57 35.30398819750677 38.0 35.0 40.0 28.0 41.0 58-59 34.90416856476225 38.0 34.0 40.0 26.5 41.0 60-61 34.45843644713505 37.0 34.0 40.0 26.0 41.0 62-63 34.0037012285785 37.0 33.5 39.5 25.5 41.0 64-65 33.542858497263275 36.0 33.0 39.0 24.5 40.5 66-67 33.011752082894944 35.5 32.0 39.0 22.5 40.0 68-69 32.455984839900935 35.0 31.5 38.0 21.5 40.0 70-71 31.844133786255554 35.0 31.0 37.5 19.5 39.5 72-73 31.370235703004504 35.0 31.0 37.0 18.5 39.0 74-75 30.812113048645976 34.0 30.0 36.0 16.5 39.0 76-77 29.615444061870594 33.0 28.5 35.0 14.0 37.0 78-79 30.310025182043745 34.0 30.0 35.0 14.0 37.0 80-81 30.117188768207264 34.0 30.0 35.0 9.5 37.0 82-83 29.649000002312402 34.0 29.5 35.0 7.0 36.5 84-85 29.13427370962259 34.0 29.0 35.0 2.0 36.0 86-87 28.59198290673394 34.0 29.0 35.0 2.0 36.0 88-89 27.946619385780124 33.0 27.0 35.0 2.0 35.0 90-91 27.393679283895747 33.0 26.0 35.0 2.0 35.0 92-93 26.732697346057705 32.5 25.0 35.0 2.0 35.0 94-95 25.965683048484106 32.0 23.5 34.0 2.0 35.0 96-97 25.10733216017537 32.0 19.5 34.0 2.0 35.0 98-99 24.04111957192838 31.0 6.0 34.0 2.0 35.0 100-101 20.508114445336002 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06190452818012915 1101 2 0.01909975733938296 1101 3 0.1410163208955737 1101 4 0.07803708782648755 1101 5 -0.010721394245635452 1101 6 0.02989859195778166 1101 7 0.011067756890049907 1101 8 0.08272313994979186 1101 9 0.1485148649997754 1101 10-11 0.13612642791419916 1101 12-13 0.10481672443829382 1101 14-15 0.25100508010640254 1101 16-17 0.24254359468746145 1101 18-19 0.23972173149028464 1101 20-21 0.22684065941133724 1101 22-23 0.1988463126974338 1101 24-25 0.22774325256386163 1101 26-27 0.185195467560078 1101 28-29 0.26559136806957184 1101 30-31 0.17862610319652816 1101 32-33 0.27382695870575446 1101 34-35 0.20982061980625843 1101 36-37 0.19376797155852188 1101 38-39 0.3038195809081401 1101 40-41 0.26135729731395685 1101 42-43 0.35517509517043067 1101 44-45 0.25363467576426046 1101 46-47 0.17582755647850234 1101 48-49 0.2036103574744601 1101 50-51 0.23124554886678084 1101 52-53 0.14229068514760712 1101 54-55 0.14963103016028612 1101 56-57 0.24904207108900067 1101 58-59 0.3802179650013997 1101 60-61 0.3679868578265868 1101 62-63 0.5203739769048994 1101 64-65 0.46588230645570405 1101 66-67 0.5713484343931441 1101 68-69 0.5860058255452643 1101 70-71 0.6556703129663397 1101 72-73 0.7585403443963052 1101 74-75 0.6736188471402969 1101 76-77 0.799319683061853 1101 78-79 0.8224508552027032 1101 80-81 0.8924926121098018 1101 82-83 1.0858599410103658 1101 84-85 1.265459639288558 1101 86-87 1.4835512195988478 1101 88-89 1.6477554133640346 1101 90-91 1.768479126076084 1101 92-93 2.07203807679781 1101 94-95 2.1972824056679308 1101 96-97 2.417373817868935 1101 98-99 2.738291703300181 1101 100-101 2.92601530188886 1102 1 0.04904799116006586 1102 2 0.08747615594963776 1102 3 0.18406208631750332 1102 4 0.031437607048360405 1102 5 0.05942029395887971 1102 6 -0.011944555467955809 1102 7 0.08177142260759496 1102 8 0.020677028179427737 1102 9 0.16354573905874048 1102 10-11 0.08607132913085991 1102 12-13 0.13403330835074456 1102 14-15 0.16239598717604764 1102 16-17 0.16676882805620608 1102 18-19 0.11170260620863104 1102 20-21 0.13077732461502478 1102 22-23 0.22316138380529082 1102 24-25 0.16318432162139374 1102 26-27 0.19364375487056407 1102 28-29 0.01622558826620235 1102 30-31 0.05435087256942239 1102 32-33 0.1154174360442255 1102 34-35 0.040529347717289 1102 36-37 0.07410134985314443 1102 38-39 0.16356709128297808 1102 40-41 0.17642419438600854 1102 42-43 0.048803070684037664 1102 44-45 0.17119495762756998 1102 46-47 0.13470429091024272 1102 48-49 0.027466962283497764 1102 50-51 0.1450847252007037 1102 52-53 0.1340335025821915 1102 54-55 0.0480748221133922 1102 56-57 0.16007864775058778 1102 58-59 0.20458853977552138 1102 60-61 0.30004322123284766 1102 62-63 0.27538394049551584 1102 64-65 0.27331396742200553 1102 66-67 0.3786624479467733 1102 68-69 0.3390524539462447 1102 70-71 0.34245553552586117 1102 72-73 0.4166987239576265 1102 74-75 0.5529275245682115 1102 76-77 0.741345074080435 1102 78-79 0.7398667673796631 1102 80-81 0.9543195086913592 1102 82-83 0.8397633017006747 1102 84-85 0.7695207308020464 1102 86-87 0.8235066639671267 1102 88-89 0.9581702517914472 1102 90-91 1.0135287381172553 1102 92-93 0.9887742483682231 1102 94-95 1.2128863626004183 1102 96-97 1.516970623874716 1102 98-99 1.9318014529969254 1102 100-101 2.351876493674503 1103 1 0.31563869153949753 1103 2 0.29994148343745053 1103 3 0.35125435838602925 1103 4 0.20707961167247646 1103 5 0.11058255691690988 1103 6 0.15581867727374998 1103 7 0.25628637149265643 1103 8 0.1908409048143085 1103 9 0.2222779333224949 1103 10-11 0.35628499038129036 1103 12-13 0.3126499513386207 1103 14-15 0.5003282540082381 1103 16-17 0.4153870091793692 1103 18-19 0.4595567557912261 1103 20-21 0.46287848112893926 1103 22-23 0.36380959709580907 1103 24-25 0.5046940423633757 1103 26-27 0.46516771322380635 1103 28-29 0.42741147237089194 1103 30-31 0.48185403606166943 1103 32-33 0.45920978595999173 1103 34-35 0.6053344459531687 1103 36-37 0.47834560209640387 1103 38-39 0.6369349006901146 1103 40-41 0.7943454728039683 1103 42-43 0.8787511411737086 1103 44-45 0.8191224644316151 1103 46-47 0.7163075254236873 1103 48-49 0.8276152543010653 1103 50-51 0.921970313722511 1103 52-53 0.9177816925428104 1103 54-55 0.9904425311018272 1103 56-57 1.0634949766797561 1103 58-59 0.9658411219163483 1103 60-61 0.971995521442885 1103 62-63 1.059585228099813 1103 64-65 0.921705645230297 1103 66-67 1.0401347983722147 1103 68-69 1.2102113251187703 1103 70-71 1.2811801088905135 1103 72-73 1.1017204717343851 1103 74-75 1.1148785778875059 1103 76-77 0.8531709984254192 1103 78-79 1.2193613306630091 1103 80-81 1.381740456273711 1103 82-83 1.3831971174381366 1103 84-85 1.5352822943484234 1103 86-87 1.644106391107389 1103 88-89 1.6065487325809862 1103 90-91 1.6183867882531864 1103 92-93 1.731796741852385 1103 94-95 2.11351630029532 1103 96-97 2.360049879708484 1103 98-99 2.659897966587277 1103 100-101 2.903609110546242 1104 1 0.0731466830623404 1104 2 0.10436982793955707 1104 3 0.1568223049868891 1104 4 0.11186111499336704 1104 5 0.07110103940008372 1104 6 0.07128949117184646 1104 7 0.07289992967156422 1104 8 0.11227709523278406 1104 9 0.1614093780921948 1104 10-11 0.10341634649656584 1104 12-13 0.06945726431847277 1104 14-15 0.14629439362997942 1104 16-17 0.16038546894905892 1104 18-19 0.19473000504874705 1104 20-21 0.21870231918087768 1104 22-23 0.19913611189407732 1104 24-25 0.16759948905541222 1104 26-27 0.21839111507354403 1104 28-29 0.28928900470310026 1104 30-31 0.3191807461241183 1104 32-33 0.3145922596540416 1104 34-35 0.43510617203794055 1104 36-37 0.47636476491810953 1104 38-39 0.39673247683331425 1104 40-41 0.3797899597052705 1104 42-43 0.4171435559462324 1104 44-45 0.5252846762135661 1104 46-47 0.6409487415379189 1104 48-49 0.5708991207111893 1104 50-51 0.5763307085376894 1104 52-53 0.5154293484469363 1104 54-55 0.7141702419492688 1104 56-57 0.7999336555941952 1104 58-59 0.8362600589498257 1104 60-61 0.982871539355564 1104 62-63 0.9769980227284805 1104 64-65 1.0248908359018287 1104 66-67 0.957139950989685 1104 68-69 1.0959296026867875 1104 70-71 1.2299991534315033 1104 72-73 1.0408569664395309 1104 74-75 1.105611244966024 1104 76-77 0.9945766255123729 1104 78-79 1.3585127226777125 1104 80-81 1.4490502041641022 1104 82-83 1.432982467126525 1104 84-85 1.6810032315491092 1104 86-87 1.7003332140078342 1104 88-89 1.760598556366297 1104 90-91 2.0152258653269826 1104 92-93 2.3515272043535305 1104 94-95 2.4789437969693786 1104 96-97 2.4439406698045474 1104 98-99 2.6953532827945956 1104 100-101 3.017760656864528 1105 1 0.1580007323942496 1105 2 0.15359263705086335 1105 3 0.22251276218085536 1105 4 0.07054247084974463 1105 5 0.12274645025144082 1105 6 0.1029202792885826 1105 7 0.1177133524471543 1105 8 0.02978324788769271 1105 9 0.04613544906229805 1105 10-11 0.04812673118907895 1105 12-13 0.0858243467611004 1105 14-15 0.16525410732891288 1105 16-17 0.1722734068412919 1105 18-19 0.15955381208515718 1105 20-21 0.19868141722579935 1105 22-23 0.09985427811054137 1105 24-25 0.15708581742646288 1105 26-27 0.16460782588443834 1105 28-29 0.14850809730080528 1105 30-31 0.21151448512125626 1105 32-33 0.24199760334912668 1105 34-35 0.26008271326278276 1105 36-37 0.3690592647526856 1105 38-39 0.25657752921967614 1105 40-41 0.25346156118513363 1105 42-43 0.28387966032571654 1105 44-45 0.27949754257173964 1105 46-47 0.4732766801051653 1105 48-49 0.46250947388986674 1105 50-51 0.5223136164996234 1105 52-53 0.5305929790451103 1105 54-55 0.5331988897152158 1105 56-57 0.5985442407115826 1105 58-59 0.7071324035754216 1105 60-61 0.5399083477533964 1105 62-63 0.5682727711966393 1105 64-65 0.690134703163821 1105 66-67 0.6743952880327271 1105 68-69 0.7802945792358642 1105 70-71 0.960744512597028 1105 72-73 0.8981336214359992 1105 74-75 0.722876766119736 1105 76-77 0.853274444699867 1105 78-79 1.078191740701822 1105 80-81 1.2728452708160667 1105 82-83 1.312483049327998 1105 84-85 1.3232209030807347 1105 86-87 1.583326220133607 1105 88-89 1.6515517498797045 1105 90-91 1.4945707621064095 1105 92-93 1.8977902440378074 1105 94-95 1.7652671250190082 1105 96-97 2.0786604615542004 1105 98-99 2.388036852861081 1105 100-101 2.8452997087100904 1106 1 0.09122969612802478 1106 2 0.05751813840659992 1106 3 0.0871527561050982 1106 4 0.05157988395658464 1106 5 0.03622267135849455 1106 6 0.046770125150679576 1106 7 -0.0038124075886827313 1106 8 -0.036647944698955826 1106 9 -0.035944470970875386 1106 10-11 0.0704479594132934 1106 12-13 0.09511510144037771 1106 14-15 0.08819129263309833 1106 16-17 0.08260770408790563 1106 18-19 0.027621202396396427 1106 20-21 -0.027281450824716558 1106 22-23 0.030273490248561075 1106 24-25 0.05458085114424449 1106 26-27 0.04113333468144731 1106 28-29 0.04294137344373894 1106 30-31 0.10714709108938791 1106 32-33 0.03613086963429879 1106 34-35 -0.06736606949463919 1106 36-37 0.08990846353093929 1106 38-39 0.17376729048908146 1106 40-41 0.2199206010078285 1106 42-43 0.15218649467416157 1106 44-45 0.16620244357020653 1106 46-47 -0.06850165359447402 1106 48-49 0.1914164391893749 1106 50-51 0.2234081547974327 1106 52-53 0.17928878097838208 1106 54-55 0.17577299845982708 1106 56-57 0.2048314063193999 1106 58-59 0.32144540344002337 1106 60-61 0.25901258732247356 1106 62-63 0.25973584810142114 1106 64-65 0.371918372332523 1106 66-67 0.3303571664707192 1106 68-69 0.03999920674839785 1106 70-71 0.2653547260886704 1106 72-73 0.3140880931229155 1106 74-75 0.5015306514420566 1106 76-77 0.5366649876097327 1106 78-79 0.8382680482657214 1106 80-81 0.847296927669074 1106 82-83 0.7207566127500762 1106 84-85 0.8138309953640182 1106 86-87 1.194280290989095 1106 88-89 1.3281012126696972 1106 90-91 1.1484219466304033 1106 92-93 1.0931169331587043 1106 94-95 1.4785221984133514 1106 96-97 1.621880254111474 1106 98-99 2.028371579706924 1106 100-101 2.0993756273332522 1107 1 0.06077557728230687 1107 2 0.08724222102006962 1107 3 0.16434459774389865 1107 4 0.048310950051543955 1107 5 0.05124535364474525 1107 6 0.06331614604060576 1107 7 -0.013441513973724284 1107 8 -0.02579202535392966 1107 9 -0.020046515402405873 1107 10-11 0.04211215594983031 1107 12-13 0.051151461315782853 1107 14-15 0.11720789476830618 1107 16-17 0.07062867323713107 1107 18-19 0.10988093685465827 1107 20-21 0.02781785500820888 1107 22-23 0.09637037656426628 1107 24-25 0.15715314233572997 1107 26-27 0.21134895668661358 1107 28-29 0.0663030830158533 1107 30-31 0.08243341162975781 1107 32-33 0.09692440306467631 1107 34-35 0.15424201272713844 1107 36-37 0.22135872149174673 1107 38-39 0.1783340054580691 1107 40-41 0.1708145240475929 1107 42-43 0.13727906678931134 1107 44-45 0.12682999965976904 1107 46-47 0.28461417625371865 1107 48-49 0.34985465677868177 1107 50-51 0.3603185556406885 1107 52-53 0.4574875994969858 1107 54-55 0.4886760450269989 1107 56-57 0.5295828254064077 1107 58-59 0.5484055259672758 1107 60-61 0.6453497419306089 1107 62-63 0.6209362653278632 1107 64-65 0.6238697201213412 1107 66-67 0.8223985612305214 1107 68-69 0.828659493987665 1107 70-71 1.007519352284806 1107 72-73 0.6969593635840106 1107 74-75 0.8663449405672132 1107 76-77 1.0115692356834174 1107 78-79 1.0988865820536908 1107 80-81 1.1209983489016295 1107 82-83 1.2337396497228106 1107 84-85 1.2727302855729512 1107 86-87 1.37487678615501 1107 88-89 1.5333819547870142 1107 90-91 1.4220116893737789 1107 92-93 1.8093094338656357 1107 94-95 1.8897023154612107 1107 96-97 1.8282599015595338 1107 98-99 2.0450166258581994 1107 100-101 2.317292267868254 1108 1 -0.021551354468741124 1108 2 0.0033482463002840746 1108 3 0.062115690883423724 1108 4 0.004298218886241045 1108 5 -0.011357483550078484 1108 6 0.032078520915405306 1108 7 -0.051056898100426906 1108 8 -0.04656699163074052 1108 9 -0.0411073611397299 1108 10-11 -0.04047709667655397 1108 12-13 -0.016899497227456095 1108 14-15 0.060062178830840196 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5638.0 14 7815.0 15 10732.0 16 14026.0 17 19247.0 18 19493.0 19 15350.0 20 14467.0 21 14375.0 22 15294.0 23 16796.0 24 19090.0 25 21966.0 26 25879.0 27 31336.0 28 37254.0 29 45234.0 30 55386.0 31 67838.0 32 85761.0 33 111637.0 34 153956.0 35 227568.0 36 348078.0 37 468136.0 38 282419.0 39 17762.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.54606649077005 18.22615741436602 14.702289970424395 23.52548612443953 2 16.016508879790265 19.371291607538986 39.601237379670934 25.010962132999815 3 20.069911666914106 22.81887948007476 29.20222722031749 27.908981632693646 4 13.26532405536075 16.218631113643447 35.73530214304147 34.78074268795433 5 14.088227744774528 36.81354333112113 33.99064398345239 15.107584940651961 6 33.751307663207 35.3499635102999 16.39280121576836 14.505927610724733 7 29.824604406048316 32.093208247870855 19.39877581506344 18.683411531017388 8 27.20618466588754 34.68616733209974 18.689088362081925 19.418559639930795 9 26.175127355469176 16.710609988183634 19.628535949737657 37.48572670660953 10-11 25.66621420692749 26.065195825654236 26.381116010831285 21.887473956586987 12-13 26.507488709703527 23.196292759179652 26.98113774739797 23.31508078371885 14-15 23.014653683307472 24.757278859338978 25.255300600530468 26.972766856823082 16-17 22.7200822104891 27.934847632413295 26.227925528655394 23.11714462844221 18-19 22.730644627946287 26.332185611780297 28.48815241495568 22.449017345317735 20-21 23.094200267776003 25.423065272134878 28.99466066675762 22.4880737933315 22-23 22.87648774080763 25.38141893532446 28.88195425608913 22.86013906777878 24-25 22.78057892672675 25.623020541964507 28.863596841746954 22.73280368956178 26-27 22.29437359603578 26.04034442688654 28.75644852100349 22.90883345607419 28-29 22.485394829825257 26.362285917249086 28.604581898098647 22.547737354827014 30-31 22.500818012697408 25.880488055858414 28.494058857603356 23.12463507384082 32-33 22.25270341707478 25.857783681309204 28.577665688791498 23.311847212824517 34-35 23.01716029169975 26.019685176863383 28.344698053384544 22.618456478052327 36-37 23.082195819963577 25.557910559939874 28.3567658196745 23.003127800422053 38-39 22.550480151524297 26.04195505941787 28.29233790621601 23.115226882841824 40-41 23.122056171781253 25.76367217676986 28.306316341664022 22.807955309784866 42-43 22.732227495276568 25.743427037146084 28.217673965500055 23.306671502077293 44-45 22.7251829307777 26.017428145900595 28.37427498358017 22.883113939741538 46-47 23.163834099720077 26.364020021251477 27.59968134344494 22.872464535583507 48-49 22.9508488615326 26.379316165714496 27.592520565392793 23.077314407360113 50-51 23.03026238810871 26.604794531634795 27.58023452368014 22.78470855657635 52-53 23.399460285673985 26.220612277460337 27.52771990352664 22.852207533339037 54-55 22.89815493547246 26.514379663823185 27.638229533519404 22.949235867184953 56-57 22.91633040506323 26.60294461106576 27.480084448873974 23.00064053499703 58-59 22.955039992970303 26.226971379416398 27.436819431565656 23.381169196047644 60-61 22.577956808979515 26.56319444283861 27.66387405740766 23.194974690774213 62-63 22.80235217400353 26.50963924236503 27.79507967376651 22.89292890986493 64-65 22.404018027475946 26.67469840513723 27.58280128846968 23.338482278917148 66-67 22.4036719226212 26.60502089031498 27.62384576038064 23.36746142668318 68-69 22.878429560709606 26.24228898759786 27.744128392273137 23.135153059419398 70-71 22.729072195462606 26.359749428258922 27.284362852670014 23.626815523608453 72-73 22.780615607317362 26.465865496900225 27.321823744192987 23.43169515158943 74-75 22.638726699672333 26.93440412902271 27.137895391616624 23.288973779688334 76-77 22.8131973333395 26.409281051494855 27.42012389843011 23.357397716735537 78-79 22.83565074424617 26.680548778936803 27.397994223623023 23.085806253194 80-81 22.985033417728427 26.60392493839174 27.137050031899633 23.2739916119802 82-83 22.882892715903157 26.755143183128904 27.26112519892648 23.100838902041456 84-85 22.866262644388787 26.867049814896994 27.185687371671435 23.081000169042785 86-87 22.97131302966004 26.865661287667024 26.92162187661291 23.241403806060024 88-89 22.80837496427339 27.031814948660056 26.720704413080053 23.439105673986496 90-91 22.73768730526011 26.92138347472619 26.935281491495385 23.405647728518314 92-93 22.882355362402755 26.56567305615588 27.084924076736765 23.467047504704592 94-95 22.682827283122577 26.470428407413106 27.162183880004765 23.684560429459555 96-97 22.887223956526768 26.12556364897676 27.22765637645971 23.75955601803677 98-99 22.61012833853625 26.4639842756388 26.845091918140824 24.080795467684123 100-101 22.873194073035343 25.976326735997734 27.099472478315928 24.051006712650995 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 465.0 1 332.5 2 304.0 3 422.0 4 350.0 5 309.5 6 677.0 7 1598.0 8 2574.5 9 2948.5 10 2466.0 11 1899.0 12 1820.0 13 1886.0 14 2058.0 15 2200.0 16 2341.0 17 2564.5 18 2815.0 19 3060.5 20 3468.5 21 4131.5 22 4867.5 23 5799.0 24 7260.5 25 9232.0 26 11765.0 27 14874.5 28 17328.0 29 19515.5 30 22400.0 31 26645.5 32 32795.5 33 35234.5 34 35110.5 35 37352.0 36 40922.0 37 44633.0 38 47757.5 39 50636.0 40 54026.5 41 58480.0 42 61797.0 43 64673.5 44 67921.0 45 71754.5 46 74219.5 47 77956.5 48 81688.5 49 81084.0 50 80284.5 51 79427.0 52 78844.5 53 80423.5 54 83289.5 55 80255.5 56 74433.5 57 70227.5 58 66187.0 59 59452.0 60 47589.0 61 38492.5 62 32317.0 63 24350.5 64 17386.5 65 13099.5 66 9702.0 67 7298.5 68 5714.0 69 4374.5 70 3269.0 71 2209.0 72 1527.5 73 1225.5 74 899.0 75 651.0 76 459.5 77 261.0 78 165.5 79 105.0 80 60.0 81 32.0 82 20.5 83 12.0 84 8.5 85 6.0 86 4.0 87 2.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01234821979831241 3 0.00453230539413714 4 4.624801422588917E-5 5 4.6248014225889176E-4 6 4.624801422588917E-5 7 0.0 8 1.849920569035567E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.3124007112944586E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.002173656668616791 26-27 0.024002719383236483 28-29 3.237360995812242E-4 30-31 1.1562003556472294E-4 32-33 0.003884833194974691 34-35 0.017319881327595498 36-37 0.008902742738483667 38-39 0.02263840296357275 40-41 0.024465199525495373 42-43 0.019678530053115845 44-45 0.010868283343083956 46-47 0.0024280207468591816 48-49 2.774880853553351E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.162321280330026E-4 68-69 3.006120924682797E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.081160640165013E-4 82-83 0.0011099523414213404 84-85 0.0037229651451840787 86-87 8.787122702918944E-4 88-89 4.624801422588917E-5 90-91 0.0035148490811675774 92-93 2.774880853553351E-4 94-95 1.1562003556472294E-4 96-97 2.3124007112944588E-4 98-99 2.3124007112944588E-4 100-101 0.003191112981586353 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2162255.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.91239046161823 #Duplication Level Percentage of deduplicated Percentage of total 1 76.27380725153068 41.12103278538731 2 13.320242832099538 14.362522652154434 3 4.396421556539385 7.110647867700804 4 1.9629060121268804 4.232998214609693 5 1.074382898379216 2.8961275161352704 6 0.6471194676779215 2.0932654450059984 7 0.4371079438718396 1.6495873900725604 8 0.30943777514737963 1.3346024125855966 9 0.22100789585602887 1.0723557578841798 >10 1.2182431369385123 11.96744189738656 >50 0.08907227371748895 3.301357560527048 >100 0.0443926670903001 4.510832692731094 >500 0.002670690584858321 0.9991513040141653 >1k 0.003015295821614212 2.6368918270172883 >5k 1.7230261837795498E-4 0.7111846767880633 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8244 0.38126862927823035 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7068 0.3268809645485847 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4314 0.19951393337048592 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2921 0.1350904495538223 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2660 0.12301971784086521 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2645 0.12232599762747687 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2436 0.11266016265426602 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2360 0.10914531357309847 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2193 0.10142189519737498 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.624801422588918E-5 7 0.0 0.0 0.0 0.0 4.624801422588918E-5 8 0.0 0.0 0.0 0.0 4.624801422588918E-5 9 0.0 0.0 0.0 0.0 4.624801422588918E-5 10-11 0.0 0.0 0.0 0.0 4.624801422588918E-5 12-13 0.0 0.0 0.0 0.0 2.5436407824239046E-4 14-15 0.0 0.0 0.0 0.0 2.7748808535533505E-4 16-17 0.0 0.0 0.0 0.0 2.7748808535533505E-4 18-19 0.0 0.0 0.0 0.0 2.7748808535533505E-4 20-21 0.0 0.0 0.0 0.0 2.7748808535533505E-4 22-23 0.0 0.0 0.0 1.3874404267766755E-4 2.7748808535533505E-4 24-25 0.0 0.0 0.0 3.4686010669416885E-4 2.7748808535533505E-4 26-27 0.0 0.0 0.0 7.86216241840116E-4 3.0061209246827963E-4 28-29 0.0 0.0 0.0 0.0028905008891180733 3.6998411380711343E-4 30-31 0.0 0.0 0.0 0.012070731712957077 3.6998411380711343E-4 32-33 0.0 0.0 0.0 0.028558148784486564 3.6998411380711343E-4 34-35 0.0 0.0 0.0 0.052098388025464154 3.6998411380711343E-4 36-37 0.0 0.0 0.0 0.09302788061537608 3.6998411380711343E-4 38-39 0.0 0.0 0.0 0.1561564200337148 3.6998411380711343E-4 40-41 0.0 0.0 0.0 0.21766627895414742 3.6998411380711343E-4 42-43 0.0 0.0 0.0 0.2827603589770864 3.6998411380711343E-4 44-45 0.0 0.0 0.0 0.36186758731046986 3.6998411380711343E-4 46-47 0.0 0.0 0.0 0.45531170005387894 3.93108120920058E-4 48-49 0.0 0.0 0.0 0.5513225775868249 4.162321280330026E-4 50-51 0.0 0.0 0.0 0.6582479864770807 4.162321280330026E-4 52-53 0.0 0.0 0.0 0.7551607002874314 5.087281564847809E-4 54-55 0.0 0.0 0.0 0.8598893285019574 5.087281564847809E-4 56-57 0.0 0.0 0.0 0.9801110414821563 5.087281564847809E-4 58-59 0.0 0.0 0.0 1.1053737880129773 5.087281564847809E-4 60-61 0.0 0.0 0.0 1.2447190548755813 5.318521635977255E-4 62-63 0.0 0.0 0.0 1.3774277316967702 5.549761707106701E-4 64-65 0.0 0.0 0.0 1.5153393101183719 5.549761707106701E-4 66-67 0.0 0.0 0.0 1.6748718351905765 5.549761707106701E-4 68-69 0.0 0.0 0.0 1.8465444639970772 5.549761707106701E-4 70-71 0.0 0.0 0.0 2.020945725642905 5.781001778236147E-4 72-73 0.0 0.0 0.0 2.202538553500859 6.474721991624484E-4 74-75 0.0 0.0 0.0 2.3829751810031654 6.937202133883376E-4 76-77 0.0 0.0 0.0 2.57682373263098 6.937202133883376E-4 78-79 0.0 0.0 0.0 2.7973805124742457 6.937202133883376E-4 80-81 0.0 0.0 0.0 3.0332222610191675 6.937202133883376E-4 82-83 0.0 0.0 0.0 3.2721626265172237 7.399682276142269E-4 84-85 0.0 0.0 0.0 3.5183176822345192 7.399682276142269E-4 86-87 0.0 0.0 0.0 3.7723349003702156 7.630922347271714E-4 88-89 0.0 0.0 0.0 4.045568168416769 7.86216241840116E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5475 0.0 39.48122 1 GTATCAA 8630 0.0 35.396755 1 TATCAAC 10530 0.0 29.009876 2 ATCAACG 10530 0.0 28.96007 3 TCAACGC 10665 0.0 28.725111 4 CAACGCA 10910 0.0 27.992975 5 AACGCAG 11185 0.0 27.304726 6 ACGCAGA 12450 0.0 24.339642 7 CGCAGAG 12670 0.0 23.954498 8 GAGTACT 7735 0.0 23.118917 12-13 TACATGG 7050 0.0 22.439772 2 GTACATG 7390 0.0 22.371653 1 GTACTTT 8170 0.0 21.713577 14-15 ACATGGG 7210 0.0 21.27944 3 GCAGAGT 14120 0.0 21.259117 9 TAAGGTG 1145 0.0 21.155708 5 GTATAGG 450 0.0 21.1145 1 GTGTAGC 1360 0.0 20.959246 1 AGAGTAC 11250 0.0 20.87741 10-11 TAAGGTA 710 0.0 20.069 4 >>END_MODULE