FastQCFastQC Report
Thu 26 May 2016
SRR937977_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937977_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2210829
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87280.3947840380237458No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70810.3202870959264602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43900.19856804845603165No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28900.13072019590841263No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28170.1274182670844285No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27460.1242068020638412No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23310.10543556285899996No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC22980.10394291010295233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51100.041.2754671
GTATCAA83350.035.6675341
CGTACGA650.00581960729.2570539
ATCAACG102700.028.7784793
TATCAAC103150.028.6522832
TCAACGC103950.028.3409944
CAACGCA106150.027.8431455
AACGCAG108750.027.2211636
GTAAGGT12950.024.2170964
CGCAGAG121550.024.2113888
ACGCAGA121550.024.172827
TAAGGTG14750.023.5168295
GTACATG70600.022.9807831
GTGTAAG9800.022.8856031
TACATGG69750.022.6849632
GAGTACT77900.022.09001712-13
ACATGGG71350.022.043573
GCAGAGT133750.021.4697579
GTACTTT81400.021.09547214-15
AGAGTAC107900.021.03904510-11
Produced by FastQC (version 0.11.5)