FastQCFastQC Report
Thu 26 May 2016
SRR937977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937977_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2210829
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85830.388225412277476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74230.3357564063073173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44880.2030007748224761No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30070.1360123284071269No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28230.12768965849461897No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26870.12153811986363487No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25070.11339637755792059No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24780.11208465240866662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50700.045.347341
GTATCAA83300.037.6368561
ATCAACG104050.029.8102653
TCAACGC105000.029.629024
TATCAAC105200.029.5308842
CAACGCA106350.029.208255
AACGCAG110250.028.1750346
TAAGGTG14600.025.0497365
ACGCAGA123950.024.9076147
CGCAGAG125450.024.4962088
GAGTACT78300.022.74755712-13
TATACCG1050.002197691222.6175735
AGGTAAG13500.022.1677282
GTAAGGT15100.022.0184334
GTACATG77600.021.9759221
GCAGAGT138700.021.9506239
ATACCGT1954.2277497E-721.9216466
TACATGG76000.021.8135432
GTACTTT82950.021.41511714-15
AGAGTAC114200.021.19060710-11
Produced by FastQC (version 0.11.5)