##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937977_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2210829 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51300665949289 34.0 31.0 34.0 31.0 34.0 2 32.729694607769304 34.0 31.0 34.0 31.0 34.0 3 32.79070837228931 34.0 31.0 34.0 31.0 34.0 4 36.245137457487665 37.0 35.0 37.0 35.0 37.0 5 36.205543260016945 37.0 37.0 37.0 35.0 37.0 6 36.24363801994637 37.0 37.0 37.0 35.0 37.0 7 36.208023777506085 37.0 37.0 37.0 35.0 37.0 8 36.19891316786599 37.0 37.0 37.0 35.0 37.0 9 37.916125580042596 39.0 38.0 39.0 35.0 39.0 10-11 37.90364926459712 39.0 38.0 39.0 35.0 39.0 12-13 37.85214121037855 39.0 38.0 39.0 35.0 39.0 14-15 39.187127769718956 40.5 39.0 41.0 36.0 41.0 16-17 39.13666751250323 40.0 39.0 41.0 36.0 41.0 18-19 39.156992693690924 40.0 39.0 41.0 36.0 41.0 20-21 39.07824644058857 40.0 39.0 41.0 36.0 41.0 22-23 38.93795019877159 40.0 38.0 41.0 35.0 41.0 24-25 38.896099381725136 40.0 38.0 41.0 35.0 41.0 26-27 38.860164445101816 40.0 38.0 41.0 35.0 41.0 28-29 38.80809280138807 40.0 38.0 41.0 35.0 41.0 30-31 38.62126944236755 40.0 38.0 41.0 34.5 41.0 32-33 38.45148086984565 40.0 38.0 41.0 34.0 41.0 34-35 38.25472006202199 40.0 38.0 41.0 34.0 41.0 36-37 38.125155315042456 40.0 38.0 41.0 33.5 41.0 38-39 38.11310214403737 40.0 38.0 41.0 34.0 41.0 40-41 38.05184910275738 40.0 38.0 41.0 33.0 41.0 42-43 37.8997898525847 40.0 38.0 41.0 33.0 41.0 44-45 37.7010625878347 40.0 37.0 41.0 33.0 41.0 46-47 37.39185821246238 40.0 37.0 41.0 32.5 41.0 48-49 37.059307164868926 40.0 36.5 41.0 31.5 41.0 50-51 36.76232716324962 40.0 36.0 41.0 31.0 41.0 52-53 36.34226120609057 39.0 35.0 41.0 30.0 41.0 54-55 36.11062954213102 39.0 35.0 41.0 30.0 41.0 56-57 35.84994949858175 39.0 35.0 40.0 29.0 41.0 58-59 35.49655287677156 38.0 35.0 40.0 28.5 41.0 60-61 35.12128255961904 38.0 34.5 40.0 28.0 41.0 62-63 34.74534846430909 37.0 34.0 40.0 27.0 41.0 64-65 34.340868968156286 37.0 34.0 39.5 26.0 41.0 66-67 33.91293198162318 36.0 33.5 39.0 26.0 41.0 68-69 33.44234221642651 35.5 33.0 39.0 25.5 40.0 70-71 32.917749631473086 35.0 32.5 38.0 24.5 40.0 72-73 32.50236359302325 35.0 32.0 37.0 23.5 39.5 74-75 32.05728733429858 35.0 32.0 37.0 22.5 39.0 76-77 30.769621938196032 34.0 30.0 35.5 21.0 38.0 78-79 31.48741264023586 34.5 31.5 36.0 22.0 38.0 80-81 31.33539161102012 35.0 32.0 35.5 21.0 37.0 82-83 30.975755022211125 34.5 31.0 35.0 20.0 37.0 84-85 30.609965311654587 34.0 31.0 35.0 18.0 36.5 86-87 30.187924981986395 34.0 31.0 35.0 15.5 36.0 88-89 29.7208207871346 34.0 30.0 35.0 8.5 36.0 90-91 29.377460219673253 34.0 30.0 35.0 4.5 35.0 92-93 28.97266658796316 34.0 29.0 35.0 2.0 35.0 94-95 28.548909029146984 34.0 29.0 35.0 2.0 35.0 96-97 27.96170260115097 34.0 29.0 35.0 2.0 35.0 98-99 27.260202846986356 33.0 27.0 35.0 2.0 35.0 100-101 24.765284651142174 31.0 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1202150660631176 1101 2 0.1338204852007081 1101 3 0.1204313030747386 1101 4 -0.005918718884814211 1101 5 0.11270472050230751 1101 6 0.09173698975970268 1101 7 0.046104893281700754 1101 8 0.07393672778364646 1101 9 -0.015776752945470207 1101 10-11 0.07837724166459026 1101 12-13 0.07967483822812937 1101 14-15 0.1468727903427407 1101 16-17 0.18855205972744926 1101 18-19 0.22788667046343392 1101 20-21 0.11328385674226382 1101 22-23 0.213385210468104 1101 24-25 0.1077090664207887 1101 26-27 0.24779134064599617 1101 28-29 0.2293073864736357 1101 30-31 0.2236587966530621 1101 32-33 0.21057616089395026 1101 34-35 0.12492764318653116 1101 36-37 0.22426107081360414 1101 38-39 0.15644824824958903 1101 40-41 0.37039644356855916 1101 42-43 0.37492336050240027 1101 44-45 0.3546608490377068 1101 46-47 0.37708727996709257 1101 48-49 0.23740726500418674 1101 50-51 0.17013578547649644 1101 52-53 0.1260751865147327 1101 54-55 0.3005314495128246 1101 56-57 0.27426199198571766 1101 58-59 0.12471441828649432 1101 60-61 0.2490604345914491 1101 62-63 0.23183265556435373 1101 64-65 0.2654985471032276 1101 66-67 0.2147682787828913 1101 68-69 0.1848140690246325 1101 70-71 0.2750575981528911 1101 72-73 0.2085065215707047 1101 74-75 0.32047201528348523 1101 76-77 0.1608952500167895 1101 78-79 0.15325265058814352 1101 80-81 0.3150990411116439 1101 82-83 0.4118997920147507 1101 84-85 0.33090968798184406 1101 86-87 0.47386055146321127 1101 88-89 0.49852715788999546 1101 90-91 0.43015037884730845 1101 92-93 0.5417128729189535 1101 94-95 0.48187438458750975 1101 96-97 0.5130143943754248 1101 98-99 0.6506401099907357 1101 100-101 0.7456112737998417 1102 1 0.17656403271108445 1102 2 0.1250618020234171 1102 3 0.14679364505170156 1102 4 0.11290597801296798 1102 5 0.0667000607102679 1102 6 0.10394737074084759 1102 7 0.16395880017215347 1102 8 0.14126640807013757 1102 9 0.13182647439197837 1102 10-11 0.20260060965872384 1102 12-13 0.14684272018006084 1102 14-15 0.19020238433679282 1102 16-17 0.24055404445369533 1102 18-19 0.20802322825779385 1102 20-21 0.2333274344270393 1102 22-23 0.23132864577033985 1102 24-25 0.24468214317791848 1102 26-27 0.1333513786146696 1102 28-29 0.08670912095178096 1102 30-31 0.1617925067967363 1102 32-33 0.197740159685857 1102 34-35 0.3567469036602873 1102 36-37 0.3284268386006346 1102 38-39 0.33943677135438577 1102 40-41 0.4221503375151414 1102 42-43 0.294973410120555 1102 44-45 0.23687166148108219 1102 46-47 0.09199322120193187 1102 48-49 0.17889020067317318 1102 50-51 0.16709937006466902 1102 52-53 0.1543467488394512 1102 54-55 0.1691080556309572 1102 56-57 0.2807662850348578 1102 58-59 0.1739334716583798 1102 60-61 0.06139593961798795 1102 62-63 0.11059716348169246 1102 64-65 0.17309551823840508 1102 66-67 0.2667594769534958 1102 68-69 0.3082715915685341 1102 70-71 0.25991327403846043 1102 72-73 0.18652352118238724 1102 74-75 0.2879127970930284 1102 76-77 0.2792507317443622 1102 78-79 0.1989121405997949 1102 80-81 0.3138370141021092 1102 82-83 0.4689984242794978 1102 84-85 0.4467896551907131 1102 86-87 0.33670949653807014 1102 88-89 0.12953526937983284 1102 90-91 0.1646932446194107 1102 92-93 0.06442935909058534 1102 94-95 0.08438441608425151 1102 96-97 -0.00839152528630649 1102 98-99 0.019480986721980287 1102 100-101 0.12907332669711025 1103 1 0.21259249252914714 1103 2 0.21122270229071916 1103 3 0.1973469968602828 1103 4 0.08956782317451939 1103 5 0.11664037409947525 1103 6 0.11034556841220677 1103 7 0.11370603772572707 1103 8 0.13045683509678696 1103 9 0.08082978561883891 1103 10-11 0.07711845914668203 1103 12-13 0.0871257054421335 1103 14-15 0.25433983087112466 1103 16-17 0.17055488972650323 1103 18-19 0.17844288464577573 1103 20-21 0.28357782493445427 1103 22-23 0.24571176049714438 1103 24-25 0.2745542352885124 1103 26-27 0.22473388698761454 1103 28-29 0.28688912955976775 1103 30-31 0.1887639331256068 1103 32-33 0.2583501016466627 1103 34-35 0.14250657966204017 1103 36-37 0.08361060349749039 1103 38-39 0.23269207651943447 1103 40-41 0.18225975721811238 1103 42-43 0.23712440517053324 1103 44-45 0.1789053533653444 1103 46-47 0.2533464210339176 1103 48-49 0.2776148306291262 1103 50-51 0.35019397851790757 1103 52-53 0.405693147675386 1103 54-55 0.4554964032864959 1103 56-57 0.44687295846370745 1103 58-59 0.3375780723920414 1103 60-61 0.33824909244244594 1103 62-63 0.4601708397887805 1103 64-65 0.3419582769846059 1103 66-67 0.28933818004624356 1103 68-69 0.48442957219453575 1103 70-71 0.47286638986067686 1103 72-73 0.42137006535349997 1103 74-75 0.25534618226402017 1103 76-77 0.27340271610854217 1103 78-79 0.25500353874139847 1103 80-81 0.24758207292188317 1103 82-83 0.3109178533925885 1103 84-85 0.44588077128720727 1103 86-87 0.4475206651794821 1103 88-89 0.4352006888378419 1103 90-91 0.42526583932231077 1103 92-93 0.5384061692897504 1103 94-95 0.3793498030251321 1103 96-97 0.3503074316642909 1103 98-99 0.5216751381241416 1103 100-101 0.5632209682565836 1104 1 0.0952942984806171 1104 2 0.052733823057764084 1104 3 0.019243039882475443 1104 4 7.061028567534322E-5 1104 5 0.04826801954514082 1104 6 -0.013552791202336323 1104 7 0.059331045269431115 1104 8 0.07444532788417746 1104 9 0.10059303204458558 1104 10-11 0.08524557372394526 1104 12-13 0.08293952400292426 1104 14-15 0.15682257192512594 1104 16-17 0.09859506341682334 1104 18-19 0.0725201745906574 1104 20-21 0.09846530205883397 1104 22-23 0.19747276191765195 1104 24-25 0.12258009571777251 1104 26-27 0.13268222106803762 1104 28-29 0.09138221994641071 1104 30-31 0.09704658319737547 1104 32-33 0.13905485598840528 1104 34-35 0.1383326762455468 1104 36-37 0.10083313162976282 1104 38-39 0.1069758411859496 1104 40-41 0.1251818308970698 1104 42-43 0.09392509790072978 1104 44-45 0.15826568503010918 1104 46-47 0.18505345742305934 1104 48-49 0.06892413399908293 1104 50-51 0.10803516083738884 1104 52-53 0.022171627631202284 1104 54-55 -0.06331488017476516 1104 56-57 0.1433853132760916 1104 58-59 0.20410680900015166 1104 60-61 0.1138696840600133 1104 62-63 0.20206786505278274 1104 64-65 0.24337699210409625 1104 66-67 0.17515610654209013 1104 68-69 0.34628111050592736 1104 70-71 0.22810057337424183 1104 72-73 0.06408990543121007 1104 74-75 0.1785091715588294 1104 76-77 0.1867859481585903 1104 78-79 0.15460573080547135 1104 80-81 0.1677422532548185 1104 82-83 0.19504515859135196 1104 84-85 0.19074585246574927 1104 86-87 0.14242335040385967 1104 88-89 0.030839410308608706 1104 90-91 0.022271687406959728 1104 92-93 0.23914903128435938 1104 94-95 0.3716984098025158 1104 96-97 0.40836822410868834 1104 98-99 0.3716179308623744 1104 100-101 0.44703008537518585 1105 1 0.11093577447910974 1105 2 0.053211057186004496 1105 3 0.0064508516208690025 1105 4 0.06572657584952424 1105 5 0.006205536774785969 1105 6 0.00943660023983739 1105 7 -0.046912957442607706 1105 8 0.003984473435096447 1105 9 0.005775002554884168 1105 10-11 -0.11449641789916853 1105 12-13 -0.03963866965217733 1105 14-15 -0.006808148463122166 1105 16-17 0.014286772522815738 1105 18-19 0.04505078208185864 1105 20-21 0.07373471190020808 1105 22-23 0.026629904489091416 1105 24-25 -0.035945846878455257 1105 26-27 -0.021322251678448367 1105 28-29 -0.12113794094071295 1105 30-31 0.03695910073093245 1105 32-33 0.008450443082452352 1105 34-35 0.05911475200616678 1105 36-37 0.10176583612773271 1105 38-39 0.03730759929373306 1105 40-41 0.10573238340243307 1105 42-43 0.05270600979044815 1105 44-45 0.05803347945160908 1105 46-47 0.07828467363286506 1105 48-49 0.10422544049123417 1105 50-51 0.09620704825719884 1105 52-53 -0.022048800420748194 1105 54-55 0.05625569930197827 1105 56-57 0.021806781988630064 1105 58-59 -0.040209418803264896 1105 60-61 0.02079902948850787 1105 62-63 0.1558894830137305 1105 64-65 0.03810455494352283 1105 66-67 -0.023909149833023946 1105 68-69 0.09738165083534511 1105 70-71 0.16290115656455129 1105 72-73 0.009854717237445243 1105 74-75 0.010422664736182696 1105 76-77 -0.026133116933085176 1105 78-79 0.02051705780260704 1105 80-81 0.007426682677696306 1105 82-83 0.10754210480271453 1105 84-85 0.10016557968546635 1105 86-87 0.11747492659014824 1105 88-89 0.027716408996116826 1105 90-91 0.0015360213908515163 1105 92-93 -0.10653543107826025 1105 94-95 -0.21134572043338196 1105 96-97 0.006255433724270176 1105 98-99 -0.03994885975967932 1105 100-101 0.1530636658742317 1106 1 -0.10345429391002625 1106 2 -0.031427179378454184 1106 3 0.027574449612991714 1106 4 -0.03752503696508569 1106 5 -0.0320826896404256 1106 6 0.019299875379068965 1106 7 0.020937622743311124 1106 8 -0.0808420583763052 1106 9 -0.12626212690219774 1106 10-11 -0.04977789163093149 1106 12-13 -0.1466518949608897 1106 14-15 -0.20150934470073167 1106 16-17 -0.09419809365210341 1106 18-19 -0.057410162393360054 1106 20-21 -0.06239578834321691 1106 22-23 -0.08782041074805136 1106 24-25 -0.0792012904616044 1106 26-27 -0.15575389764217107 1106 28-29 0.02815443521939187 1106 30-31 0.07558385482153795 1106 32-33 -0.022388579171106926 1106 34-35 0.05002488003930239 1106 36-37 0.05547048310943836 1106 38-39 0.03173619666775096 1106 40-41 0.05134453182258625 1106 42-43 -0.028576298011039114 1106 44-45 0.145275322280213 1106 46-47 0.14992853501703962 1106 48-49 0.0950543936230801 1106 50-51 0.14208975424494952 1106 52-53 0.08091865254549901 1106 54-55 -0.2555859350473568 1106 56-57 0.002555166884718574 1106 58-59 -0.004850070583351851 1106 60-61 0.05821140638118294 1106 62-63 0.14908872418579477 1106 64-65 -0.12950301563190436 1106 66-67 -0.1595776876240933 1106 68-69 -0.24253104655988977 1106 70-71 -0.17115826766025322 1106 72-73 -0.09846674987060311 1106 74-75 -0.08995215154717329 1106 76-77 0.006478208100762828 1106 78-79 0.021964088651742486 1106 80-81 -0.28833955497341535 1106 82-83 -0.20309363870978103 1106 84-85 -0.03982172993983468 1106 86-87 0.11831443341304038 1106 88-89 0.06415335164600222 1106 90-91 -0.09848891294427986 1106 92-93 0.01709690060830482 1106 94-95 -0.2445246974502986 1106 96-97 -0.1241235719852547 1106 98-99 0.3373601894008331 1106 100-101 0.5243938163120241 1107 1 0.09792741303715502 1107 2 0.09516055374182741 1107 3 0.024644032456230036 1107 4 0.09310757805078396 1107 5 0.0791925272973657 1107 6 0.062270757999627335 1107 7 0.048514614391301336 1107 8 0.05311582647096458 1107 9 0.08419009499945673 1107 10-11 0.08544638838711904 1107 12-13 0.1772298489748394 1107 14-15 0.1929650296381027 1107 16-17 0.16756620426840385 1107 18-19 0.17786072318627788 1107 20-21 0.11921877996351071 1107 22-23 0.13522012129512007 1107 24-25 0.14636204501706374 1107 26-27 0.1091313084209915 1107 28-29 0.12107408871074199 1107 30-31 0.11749594045740253 1107 32-33 0.08512656763640791 1107 34-35 0.1380085976835801 1107 36-37 0.05558986362535023 1107 38-39 0.09455013690906355 1107 40-41 0.09781258851861452 1107 42-43 0.04743274938608266 1107 44-45 0.16293707316978612 1107 46-47 0.09992733288945743 1107 48-49 0.1107391069988779 1107 50-51 0.1112637816312656 1107 52-53 0.1805399823575229 1107 54-55 0.19392794305321104 1107 56-57 0.25607028040255386 1107 58-59 0.07041689421095754 1107 60-61 0.007364923621544506 1107 62-63 0.1970377095901128 1107 64-65 0.007954540017358624 1107 66-67 0.07739291388356406 1107 68-69 0.2548323177698393 1107 70-71 0.26234484307604333 1107 72-73 0.16509435889790325 1107 74-75 0.11484332031564293 1107 76-77 0.12776344893314828 1107 78-79 0.18941176836056428 1107 80-81 0.26745580962263205 1107 82-83 0.3421390007185252 1107 84-85 0.4535027631322137 1107 86-87 0.43071289986813355 1107 88-89 0.4392781879717056 1107 90-91 0.586320540587014 1107 92-93 0.7330663332780638 1107 94-95 0.5679819604870744 1107 96-97 0.7050072157966909 1107 98-99 0.7972131044677475 1107 100-101 0.7247876518017904 1108 1 0.08559080048584633 1108 2 -0.017934848196347275 1108 3 0.0034089398692458417 1108 4 0.011839307925434639 1108 5 0.020493057440667428 1108 6 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0.3016632482025976 2316 100-101 0.3972937282929827 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 12.0 5 42.0 6 64.0 7 185.0 8 452.0 9 828.0 10 1431.0 11 2311.0 12 3599.0 13 5219.0 14 7175.0 15 9820.0 16 12179.0 17 17503.0 18 17491.0 19 13281.0 20 11413.0 21 11189.0 22 11837.0 23 12583.0 24 14345.0 25 16896.0 26 19863.0 27 24260.0 28 29688.0 29 36051.0 30 44221.0 31 55026.0 32 69530.0 33 90057.0 34 126819.0 35 193954.0 36 325279.0 37 533724.0 38 444517.0 39 47973.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.54565640309585 18.2657274714598 14.671329171093738 23.51728695435061 2 16.045192884043384 19.279763857002298 39.625202157858425 25.049841101095893 3 19.87664401144447 22.812896513508317 29.268345641037243 28.042113834009974 4 13.267918957097088 16.213691787107912 35.750028609177825 34.768360646617175 5 14.109594183901153 36.80759570278841 33.959885635659745 15.12292447765069 6 33.78673791595822 35.296804954159725 16.42067296927985 14.495784160602199 7 29.8150603235257 32.11813306230378 19.425428199105404 18.64137841506512 8 27.114361587844517 34.72822992001614 18.727567722579078 19.429840769560265 9 26.246763086178238 16.76826524035145 19.575543066502323 37.409428606967985 10-11 25.686179256740342 26.056244060485906 26.389603176003213 21.86797350677054 12-13 26.531947970648112 23.168006209435465 26.975763390112938 23.324282429803482 14-15 22.997278396474808 24.701277213208257 25.263554983221226 27.03788940709571 16-17 22.7197313220178 27.918265805751247 26.2215914873407 23.140411384890257 18-19 22.713154205956226 26.350816820296817 28.484948406231325 22.451080567515625 20-21 23.051850685873944 25.426638604794853 29.05102565598696 22.470485053344245 22-23 22.906407505962694 25.34791247988877 28.901059285905873 22.844620728242663 24-25 22.790905015712127 25.591675626822656 28.926029746963987 22.69138961050123 26-27 22.250503256378106 25.999947067314316 28.756247018295028 22.99330265801255 28-29 22.48489141403519 26.425992240919584 28.592102781354868 22.497013563690363 30-31 22.524921647038283 25.91600254926998 28.464254811204302 23.094820992487435 32-33 22.30915835017664 25.8098753923038 28.594341869312203 23.286624388207354 34-35 23.048606147248034 25.9883642022783 28.359384189430063 22.6036454610436 36-37 23.098155013597207 25.559018938386462 28.383108341611074 22.959717706405254 38-39 22.618147666090763 26.01019800192994 28.273600692714957 23.098053639264343 40-41 23.12072452402726 25.7693340351355 28.327421019547845 22.782520421289394 42-43 22.76334077078878 25.755097748068167 28.22488332091244 23.25667816023061 44-45 22.748524044994237 26.040858125577852 28.354597398555796 22.85602043087211 46-47 23.16425720041152 26.372307937951767 27.63346410632937 22.829970755307343 48-49 22.938906627333004 26.313206494034592 27.61034435499082 23.137542523641585 50-51 22.98063305664979 26.630689212055746 27.582617199249693 22.806060532044768 52-53 23.444983759485698 26.176515687101986 27.53632234786137 22.84217820555095 54-55 22.88797550602059 26.487439779376874 27.682217846789598 22.942366867812932 56-57 22.92649047031679 26.58952818151019 27.509251054694868 22.97473029347815 58-59 22.89114173913948 26.18635362572139 27.440905651228565 23.48159898391056 60-61 22.57008570088415 26.55110368101739 27.68610779033566 23.192702827762798 62-63 22.738031751890354 26.536652088424752 27.860770778744083 22.864545380940815 64-65 22.400986236384632 26.62783960224875 27.679458700785997 23.291715460580622 66-67 22.404559556618807 26.579848554546732 27.648904551188718 23.366687337645743 68-69 22.933814300091594 26.263679848020537 27.745886716497235 23.056619135390637 70-71 22.721228100409395 26.446753683799155 27.293811506905328 23.538206708886122 72-73 22.787560684250117 26.479976515596636 27.338025690815527 23.39443710933772 74-75 22.63304850804834 26.943105957086686 27.21666849855869 23.20717703630629 76-77 22.816079398270965 26.419071759959728 27.39632961210478 23.36851922966453 78-79 22.86323365579156 26.654458576398266 27.400083859945752 23.082223907864424 80-81 22.99289090201006 26.710433054750048 27.107885774974005 23.188790268265887 82-83 22.875129645938244 26.815438914542916 27.226302893620446 23.083128545898393 84-85 22.839443069603636 26.911638119181585 27.177994364836362 23.07092444637842 86-87 22.910771895979888 26.913445109229713 26.908695745587895 23.267087249202504 88-89 22.82035184118884 27.08416352794678 26.723936336945403 23.371548293918973 90-91 22.76675107725842 26.980005839460958 26.864641430636272 23.388601652644347 92-93 22.8686137115157 26.623350923333668 27.11350831060233 23.394527054548302 94-95 22.700150034217934 26.502592466445847 27.20117657222698 23.59608092710924 96-97 22.928390210188123 26.22742419246355 27.23446725187701 23.609718345471315 98-99 22.622645170657705 26.509761722865044 26.859924489863307 24.00766861661395 100-101 22.69741350416518 26.234005434160668 27.23761086904505 23.8309701926291 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 496.0 1 350.0 2 314.0 3 433.5 4 357.5 5 327.5 6 673.5 7 1556.5 8 2580.5 9 3073.5 10 2578.0 11 1897.5 12 1831.5 13 1971.0 14 2123.5 15 2224.0 16 2354.5 17 2585.5 18 2780.5 19 3114.5 20 3623.5 21 4170.5 22 4967.0 23 6105.5 24 7452.5 25 9301.5 26 11835.5 27 14931.5 28 17625.5 29 20051.5 30 22957.0 31 27047.5 32 33571.5 33 36319.5 34 36148.5 35 38279.0 36 41706.0 37 45690.5 38 48846.5 39 51756.0 40 55556.0 41 60441.0 42 63471.5 43 66109.0 44 69452.0 45 73414.5 46 75849.0 47 79710.5 48 83604.0 49 82912.5 50 82425.5 51 81347.5 52 80432.5 53 82534.0 54 85509.5 55 82359.0 56 76383.0 57 71939.0 58 68037.5 59 60668.0 60 48242.5 61 39039.5 62 32926.5 63 24614.5 64 17303.0 65 13141.5 66 9761.0 67 7368.0 68 5678.5 69 4389.5 70 3223.0 71 2055.5 72 1443.5 73 1148.5 74 866.0 75 618.5 76 433.0 77 276.5 78 166.5 79 96.5 80 51.0 81 31.0 82 15.5 83 8.0 84 5.5 85 3.0 86 2.5 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013750498116317454 3 0.0069657128615555515 4 0.0 5 0.0 6 0.0 7 0.0 8 3.618552135873014E-4 9 1.809276067936507E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.809276067936507E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00524690059701587 26-27 0.021462537355896816 28-29 0.0 30-31 0.0 32-33 4.7493496783333305E-4 34-35 0.018499847794650786 36-37 0.003663784037571427 38-39 0.01791183307257142 40-41 0.014903911609626976 42-43 0.007757271141277774 44-45 0.0025782183968095225 46-47 2.2615950849206337E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.809276067936507E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0030757693154920616 86-87 2.940073610396824E-4 88-89 9.046380339682535E-5 90-91 3.844711644365077E-4 92-93 3.844711644365077E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2210829.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.235755632547786 #Duplication Level Percentage of deduplicated Percentage of total 1 75.5229281368219 40.205201469463184 2 13.8511158654352 14.747492389008276 3 4.518487345843717 7.216352645162866 4 2.05049058098725 4.366376619851126 5 1.0595036166808345 2.8201737814710746 6 0.6338742851762313 2.024686592843866 7 0.4425581542679424 1.6491942427659698 8 0.29781154576705726 1.2683378140005106 9 0.22536244452575058 1.0797606022973816 >10 1.2518316100573073 12.1745701938162 >50 0.09391815166005595 3.433109276914157 >100 0.045830289061152066 4.515952083557043 >500 0.003059014240052848 1.092761586712326 >1k 0.003059014240052743 2.681986244756608 >5k 1.699452355584857E-4 0.7240444573795185 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8583 0.388225412277476 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7423 0.3357564063073173 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4488 0.2030007748224761 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3007 0.1360123284071269 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2823 0.12768965849461897 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2687 0.12153811986363487 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2507 0.11339637755792059 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2478 0.11208465240866662 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.5231901698412674E-5 7 0.0 0.0 0.0 0.0 4.5231901698412674E-5 8 0.0 0.0 0.0 0.0 4.5231901698412674E-5 9 0.0 0.0 0.0 0.0 4.5231901698412674E-5 10-11 0.0 0.0 0.0 0.0 4.5231901698412674E-5 12-13 0.0 0.0 0.0 0.0 4.5231901698412674E-5 14-15 0.0 0.0 0.0 0.0 4.5231901698412674E-5 16-17 0.0 0.0 0.0 0.0 4.5231901698412674E-5 18-19 0.0 0.0 0.0 2.2615950849206337E-5 4.5231901698412674E-5 20-21 0.0 0.0 0.0 1.1307975424603169E-4 4.5231901698412674E-5 22-23 0.0 0.0 0.0 2.487754593412697E-4 4.5231901698412674E-5 24-25 0.0 0.0 0.0 5.653987712301585E-4 4.5231901698412674E-5 26-27 0.0 0.0 0.0 9.272539848174598E-4 4.5231901698412674E-5 28-29 0.0 0.0 0.0 0.003121001217190475 4.5231901698412674E-5 30-31 0.0 0.0 0.0 0.01056164904657936 4.5231901698412674E-5 32-33 0.0 0.0 0.0 0.026483278444420624 4.5231901698412674E-5 34-35 0.0 0.0 0.0 0.05122512867345236 4.5231901698412674E-5 36-37 0.0 0.0 0.0 0.09159460093928568 4.5231901698412674E-5 38-39 0.0 0.0 0.0 0.15415032098819043 4.5231901698412674E-5 40-41 0.0 0.0 0.0 0.2173619036117221 4.5231901698412674E-5 42-43 0.0 0.0 0.0 0.28342309604225385 4.5231901698412674E-5 44-45 0.0 0.0 0.0 0.36212660499749194 4.5231901698412674E-5 46-47 0.0 0.0 0.0 0.4549876991843331 6.784785254761901E-5 48-49 0.0 0.0 0.0 0.5493414461272219 9.046380339682535E-5 50-51 0.0 0.0 0.0 0.6505930580791187 9.046380339682535E-5 52-53 0.0 0.0 0.0 0.7476833350747616 9.046380339682535E-5 54-55 0.0 0.0 0.0 0.8555614206254758 1.3569570509523802E-4 56-57 0.0 0.0 0.0 0.9753354963228726 1.3569570509523802E-4 58-59 0.0 0.0 0.0 1.1034096259819282 1.3569570509523802E-4 60-61 0.0 0.0 0.0 1.2394445703399042 1.3569570509523802E-4 62-63 0.0 0.0 0.0 1.3705492374127535 1.3569570509523802E-4 64-65 0.0 0.0 0.0 1.5036441081603327 1.3569570509523802E-4 66-67 0.0 0.0 0.0 1.6594906254622135 1.3569570509523802E-4 68-69 0.0 0.0 0.0 1.8300148948652293 1.3569570509523802E-4 70-71 0.0 0.0 0.0 2.007640572834896 1.3569570509523802E-4 72-73 0.0 0.0 0.0 2.19132732563215 1.3569570509523802E-4 74-75 0.0 0.0 0.0 2.373295266164864 1.3569570509523802E-4 76-77 0.0 0.0 0.0 2.5711848360954193 1.3569570509523802E-4 78-79 0.0 0.0 0.0 2.7922783715972606 1.3569570509523802E-4 80-81 0.0 0.0 0.0 3.0221921279302926 1.3569570509523802E-4 82-83 0.0 0.0 0.0 3.260654713684324 2.2615950849206337E-4 84-85 0.0 0.0 0.0 3.500949191457141 2.2615950849206337E-4 86-87 0.0 0.0 0.0 3.747440439762641 2.2615950849206337E-4 88-89 0.0 0.0 0.0 4.016389327261403 2.487754593412697E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5070 0.0 45.34734 1 GTATCAA 8330 0.0 37.636856 1 ATCAACG 10405 0.0 29.810265 3 TCAACGC 10500 0.0 29.62902 4 TATCAAC 10520 0.0 29.530884 2 CAACGCA 10635 0.0 29.20825 5 AACGCAG 11025 0.0 28.175034 6 TAAGGTG 1460 0.0 25.049736 5 ACGCAGA 12395 0.0 24.907614 7 CGCAGAG 12545 0.0 24.496208 8 GAGTACT 7830 0.0 22.747557 12-13 TATACCG 105 0.0021976912 22.617573 5 AGGTAAG 1350 0.0 22.167728 2 GTAAGGT 1510 0.0 22.018433 4 GTACATG 7760 0.0 21.975922 1 GCAGAGT 13870 0.0 21.950623 9 ATACCGT 195 4.2277497E-7 21.921646 6 TACATGG 7600 0.0 21.813543 2 GTACTTT 8295 0.0 21.415117 14-15 AGAGTAC 11420 0.0 21.190607 10-11 >>END_MODULE