FastQCFastQC Report
Thu 26 May 2016
SRR937976_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937976_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2188524
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82270.37591545717570385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69830.31907349428199094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41620.19017383405436722No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30700.14027719138560965No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27790.12698055858651766No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26950.12314235530430555No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23470.10724122742085535No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC22540.10299178807269191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45650.051.0186121
GTATCAA76400.039.211891
TATCAAC97500.030.4872742
ATCAACG98100.030.3008083
TCAACGC99500.029.7788684
CAACGCA101300.029.2966795
AACGCAG105000.028.3956
CGCAGAG115700.025.6374978
ACGCAGA116200.025.6107587
TACATGG73550.023.8609052
GTACATG75900.023.8452761
GAGTACT74400.022.74477212-13
GCAGAGT127400.022.6476829
ACATGGG74150.022.4491623
TAAGGTG12850.021.8369565
AGAGTAC107300.021.77300510-11
CAGAGTA119250.021.60406310-11
GTACTTT78300.021.42731314-15
GTGTAAG9150.021.341421
GTAAGGT11950.021.0936224
Produced by FastQC (version 0.11.5)