##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937976_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2188524 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94202896564077 33.0 31.0 34.0 30.0 34.0 2 32.04703946586832 33.0 31.0 34.0 30.0 34.0 3 32.075239293697486 34.0 31.0 34.0 30.0 34.0 4 35.58959280318607 37.0 35.0 37.0 33.0 37.0 5 35.59236407734162 37.0 35.0 37.0 33.0 37.0 6 35.62303177849546 37.0 35.0 37.0 33.0 37.0 7 35.554483295591 37.0 35.0 37.0 33.0 37.0 8 35.54611007235927 37.0 35.0 37.0 33.0 37.0 9 37.17588932083907 39.0 37.0 39.0 34.0 39.0 10-11 37.083882561945856 39.0 37.0 39.0 33.0 39.0 12-13 36.92980908594103 39.0 37.0 39.0 33.0 39.0 14-15 38.1110778314517 40.0 38.0 41.0 33.0 41.0 16-17 37.92750182314656 40.0 38.0 41.0 33.0 41.0 18-19 38.101789151044265 40.0 38.0 41.0 33.0 41.0 20-21 38.09146278496375 40.0 38.0 41.0 33.0 41.0 22-23 37.97684421098421 40.0 38.0 41.0 33.0 41.0 24-25 37.85153441314786 40.0 38.0 41.0 32.5 41.0 26-27 37.66270212252641 40.0 38.0 41.0 32.0 41.0 28-29 37.48171964301054 40.0 37.0 41.0 32.0 41.0 30-31 37.258965631631185 40.0 37.0 41.0 31.0 41.0 32-33 36.96808214120567 40.0 36.5 41.0 30.5 41.0 34-35 36.72918962734701 39.0 36.0 41.0 30.0 41.0 36-37 36.44331636299168 39.0 35.5 41.0 30.0 41.0 38-39 36.1342939350905 39.0 35.0 40.5 29.5 41.0 40-41 36.02392411506568 39.0 35.0 40.5 29.0 41.0 42-43 35.789596550003566 39.0 35.0 40.5 27.5 41.0 44-45 35.45723876000446 39.0 35.0 40.0 26.5 41.0 46-47 35.08146837777424 38.0 34.5 40.0 25.0 41.0 48-49 34.6578349152214 38.0 34.0 40.0 24.0 41.0 50-51 33.91955560002997 37.5 33.5 39.5 23.0 40.5 52-53 33.981823594349436 38.0 33.5 39.5 23.0 40.5 54-55 34.0446241393743 38.0 33.5 40.0 22.5 41.0 56-57 33.70878523607692 38.0 33.0 40.0 20.5 41.0 58-59 33.26876081779318 37.0 33.0 40.0 18.5 41.0 60-61 32.73814543500551 36.5 32.0 39.5 14.5 41.0 62-63 32.16255818990333 36.0 31.0 39.0 9.0 41.0 64-65 31.595426186781594 35.5 31.0 39.0 7.0 40.0 66-67 30.995967144979907 35.0 30.0 38.5 2.0 40.0 68-69 30.29409821413884 35.0 29.0 38.0 2.0 40.0 70-71 29.681745093953733 34.0 28.5 37.0 2.0 39.5 72-73 28.967215346964437 34.0 27.5 36.5 2.0 39.0 74-75 28.282742615571042 34.0 26.0 36.0 2.0 39.0 76-77 27.525391542427684 33.0 25.5 35.0 2.0 37.5 78-79 26.744989773929824 32.5 24.5 35.0 2.0 37.0 80-81 26.01496350965308 32.0 21.5 35.0 2.0 36.5 82-83 25.25787562759193 31.5 19.0 35.0 2.0 36.0 84-85 24.511952347792395 31.0 14.5 34.0 2.0 35.5 86-87 23.755414836666173 31.0 7.0 34.0 2.0 35.0 88-89 22.85335618892002 30.0 2.0 34.0 2.0 35.0 90-91 21.92564532077327 29.0 2.0 34.0 2.0 35.0 92-93 20.971134883601916 29.0 2.0 34.0 2.0 35.0 94-95 19.86474240172829 26.5 2.0 33.5 2.0 35.0 96-97 18.58043914528696 25.0 2.0 33.0 2.0 35.0 98-99 17.006222687071286 12.5 2.0 32.5 2.0 34.0 100-101 13.149873613449063 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26632776760154186 1101 2 0.13559717279551364 1101 3 0.1483076245816406 1101 4 0.06672925501254667 1101 5 0.10182050282094934 1101 6 0.10537345513727558 1101 7 0.09677904903247025 1101 8 0.16740882681014568 1101 9 0.11312898804558102 1101 10-11 0.200384190772958 1101 12-13 0.14321352111538488 1101 14-15 0.13732043826458806 1101 16-17 0.14651620534342413 1101 18-19 0.235192266976064 1101 20-21 0.28123335700496455 1101 22-23 0.26561265442468596 1101 24-25 0.2662257689732215 1101 26-27 0.0857308835623698 1101 28-29 0.17765174932967653 1101 30-31 0.16584730071357257 1101 32-33 0.1139766076706934 1101 34-35 0.07278834288016611 1101 36-37 -0.0406158163650332 1101 38-39 0.02726840253424001 1101 40-41 0.15487349461410815 1101 42-43 0.11590754802704595 1101 44-45 0.03706513974779568 1101 46-47 0.186102417676409 1101 48-49 -0.05528225550429511 1101 50-51 -0.11228513627082037 1101 52-53 -0.1918767705941704 1101 54-55 -0.17602468227423174 1101 56-57 -0.23826686617870507 1101 58-59 -0.16039310295763443 1101 60-61 0.03680527572574732 1101 62-63 -0.05362617704693662 1101 64-65 -0.3017213347500274 1101 66-67 -0.46057853835521456 1101 68-69 -0.43608340534743206 1101 70-71 -0.4942878370238333 1101 72-73 -0.4749476199703331 1101 74-75 -0.5316617448596901 1101 76-77 -0.6081363221707825 1101 78-79 -0.6162601094427735 1101 80-81 -0.47988402433215427 1101 82-83 -0.6438960789462378 1101 84-85 -0.5545651361467776 1101 86-87 -0.44719680217988866 1101 88-89 -0.6929588927585648 1101 90-91 -0.8448949370561287 1101 92-93 -0.9418044097689311 1101 94-95 -1.1449168326611634 1101 96-97 -1.2748778291495775 1101 98-99 -0.8497088015339749 1101 100-101 -0.8038875408164987 1102 1 0.24336654255843726 1102 2 0.2908624896487737 1102 3 0.32485180962915905 1102 4 0.2346179992776385 1102 5 0.1520064728521362 1102 6 0.2780386187075692 1102 7 0.186225880617215 1102 8 0.3289519379379442 1102 9 0.2978380144874748 1102 10-11 0.357495710586484 1102 12-13 0.4569400929406626 1102 14-15 0.44217162116073894 1102 16-17 0.38639524559957295 1102 18-19 0.3566539949422278 1102 20-21 0.42954695095063755 1102 22-23 0.5004976745944205 1102 24-25 0.4443328299659939 1102 26-27 0.46079575887618063 1102 28-29 0.26805560182592103 1102 30-31 0.4458234514801731 1102 32-33 0.41219812620490615 1102 34-35 0.43785872276606597 1102 36-37 0.4480857266756715 1102 38-39 0.25784647649737735 1102 40-41 0.3658227809563144 1102 42-43 0.38087323541194706 1102 44-45 0.4355888815892328 1102 46-47 0.5744248611899962 1102 48-49 0.3578908075377285 1102 50-51 0.43896618558937206 1102 52-53 0.384091605697904 1102 54-55 0.29816967254649285 1102 56-57 0.4462402868307933 1102 58-59 0.33849236790627657 1102 60-61 0.312273663167808 1102 62-63 0.3600346685445359 1102 64-65 0.20474119718881312 1102 66-67 -0.03618694614893769 1102 68-69 -0.15001947954296213 1102 70-71 -0.43421934089874625 1102 72-73 -0.38515584302639283 1102 74-75 -0.37216060697426556 1102 76-77 -0.5311117487751211 1102 78-79 -0.30265301852109516 1102 80-81 -0.2170201680097037 1102 82-83 -0.4544863357362843 1102 84-85 -0.4284474087252086 1102 86-87 -0.4398288913703894 1102 88-89 -0.4672882210951421 1102 90-91 -0.4416572158316008 1102 92-93 -0.687663363618551 1102 94-95 -0.8234205984222562 1102 96-97 -1.0021116114948079 1102 98-99 -0.9855128152942321 1102 100-101 -1.1086320359509987 1103 1 0.27613179844326297 1103 2 0.25346573040582143 1103 3 0.2567859127966301 1103 4 0.21289008788544805 1103 5 0.19546524983619662 1103 6 0.21087375277710407 1103 7 0.2990606283683306 1103 8 0.2748428166191914 1103 9 0.3122940497469884 1103 10-11 0.43351408570848804 1103 12-13 0.3638810987093066 1103 14-15 0.4616290028535701 1103 16-17 0.3883779680763766 1103 18-19 0.33365680029569234 1103 20-21 0.47320774974350854 1103 22-23 0.47597270422163973 1103 24-25 0.5127751600114507 1103 26-27 0.30642769059326724 1103 28-29 0.42835774827923956 1103 30-31 0.46506154065836824 1103 32-33 0.4129596279836747 1103 34-35 0.29854684676972454 1103 36-37 0.38206618123610525 1103 38-39 0.32660513315460804 1103 40-41 0.45649216851663255 1103 42-43 0.5597090080431073 1103 44-45 0.5677323920647126 1103 46-47 0.6045063402896034 1103 48-49 0.5087162222903245 1103 50-51 0.4613212962980029 1103 52-53 0.5707885036746205 1103 54-55 0.45991415018149695 1103 56-57 0.44767739135151174 1103 58-59 0.6968330393958624 1103 60-61 0.586845389431673 1103 62-63 0.43493692430323705 1103 64-65 0.3225087766653054 1103 66-67 0.10278781021480654 1103 68-69 0.06206217453616247 1103 70-71 -0.05627323686319485 1103 72-73 -0.007664344872200246 1103 74-75 0.08193510682286842 1103 76-77 0.13090417436114876 1103 78-79 -0.2207397651859715 1103 80-81 -0.0699021602310701 1103 82-83 -0.17254081345082284 1103 84-85 -0.29073330026290023 1103 86-87 -0.6037894732568247 1103 88-89 -0.39857228671018774 1103 90-91 -0.32902426264316276 1103 92-93 -0.36801611778482624 1103 94-95 -0.5957612221901556 1103 96-97 -0.8758087810306456 1103 98-99 -1.039197213597614 1103 100-101 -1.2037519253275821 1104 1 0.2522053638575521 1104 2 0.2010267918694666 1104 3 0.22338434049724043 1104 4 0.14373185372758002 1104 5 0.1871884131883803 1104 6 0.17832447211081615 1104 7 0.2513622544414744 1104 8 0.24361680270135366 1104 9 0.2561153419734552 1104 10-11 0.30539729928660364 1104 12-13 0.276113248998513 1104 14-15 0.22539614471079972 1104 16-17 0.28092759539404 1104 18-19 0.33149889545662603 1104 20-21 0.22760460523031867 1104 22-23 0.3857411689176544 1104 24-25 0.39136040725627197 1104 26-27 0.32835873992197406 1104 28-29 0.2524155894157616 1104 30-31 0.37505789653923927 1104 32-33 0.32796809205486 1104 34-35 0.33912292572749436 1104 36-37 0.4003165204516037 1104 38-39 0.19203902620153457 1104 40-41 0.40825605698647394 1104 42-43 0.2803030743558068 1104 44-45 0.28049844967345905 1104 46-47 0.19845079004794286 1104 48-49 0.09321495690578985 1104 50-51 0.17440372678637317 1104 52-53 0.31877316456505156 1104 54-55 0.42759468654105603 1104 56-57 0.3759477314956854 1104 58-59 0.4369175795360647 1104 60-61 0.3693380182752506 1104 62-63 0.4099520261063958 1104 64-65 0.27143568736170565 1104 66-67 0.3029697807909528 1104 68-69 0.27387119615971756 1104 70-71 0.3126861221142079 1104 72-73 0.28943963962863606 1104 74-75 0.16899499598429912 1104 76-77 0.18033762403667808 1104 78-79 0.02369054215899169 1104 80-81 -0.03510467418111318 1104 82-83 -0.09532207878399745 1104 84-85 -0.268358130601662 1104 86-87 0.04548802609427227 1104 88-89 0.20200047980106106 1104 90-91 0.11820726602736542 1104 92-93 -0.21240088968928816 1104 94-95 -0.5161744520330735 1104 96-97 -0.9552036090348537 1104 98-99 -1.1608322611648152 1104 100-101 -1.0037643577011401 1105 1 0.25513733131503713 1105 2 0.24917221679239532 1105 3 0.28001966751457985 1105 4 0.1833252234867544 1105 5 0.1566809934585507 1105 6 0.20087360566594725 1105 7 0.30297250580299107 1105 8 0.2548555278756055 1105 9 0.2000284122045599 1105 10-11 0.3169379847893836 1105 12-13 0.27512201113105306 1105 14-15 0.32983329376608594 1105 16-17 0.32378337976267346 1105 18-19 0.39874261193670435 1105 20-21 0.4216070204985982 1105 22-23 0.40082033224617675 1105 24-25 0.393933440738337 1105 26-27 0.43948770277577154 1105 28-29 0.3204018278974985 1105 30-31 0.3120998413667806 1105 32-33 0.4347821816584627 1105 34-35 0.3140664337668966 1105 36-37 0.28316399288308247 1105 38-39 0.2673487608431486 1105 40-41 0.2607840191344266 1105 42-43 0.30997204918029553 1105 44-45 0.298153443372172 1105 46-47 0.23568141087630323 1105 48-49 0.2931793017616826 1105 50-51 0.23644025911746525 1105 52-53 0.004331360244869131 1105 54-55 0.19520777871219508 1105 56-57 0.3476039636978925 1105 58-59 0.1865838693479489 1105 60-61 0.12386260634024637 1105 62-63 0.20464987206747054 1105 64-65 0.1675262178650847 1105 66-67 0.2456622683287435 1105 68-69 0.24511535351222946 1105 70-71 0.08765658386800723 1105 72-73 -0.011984722261342284 1105 74-75 -0.13524210305848428 1105 76-77 -0.28835152422387367 1105 78-79 -0.45619361695037597 1105 80-81 -0.5786316081101184 1105 82-83 -0.5773139520630046 1105 84-85 -0.4562532816885181 1105 86-87 -0.3466439754230919 1105 88-89 -0.21672733802746436 1105 90-91 -0.3713253283888456 1105 92-93 -0.5641183758130985 1105 94-95 -0.8471472117930219 1105 96-97 -1.1326267966461714 1105 98-99 -1.4433184792396325 1105 100-101 -1.1158000302481081 1106 1 0.20346684467479648 1106 2 0.1554366111363663 1106 3 0.21194370474960067 1106 4 0.17209162684939372 1106 5 0.17162562859974884 1106 6 0.24765191749125393 1106 7 0.2714726390129485 1106 8 0.09650307052029916 1106 9 0.3600206925488365 1106 10-11 0.24604541046534933 1106 12-13 0.41438377919791236 1106 14-15 0.4142422703054365 1106 16-17 -0.1306607482628408 1106 18-19 0.3168081038920505 1106 20-21 0.44452468805148015 1106 22-23 0.34779310886576553 1106 24-25 0.21971414406274192 1106 26-27 0.15068779765314133 1106 28-29 0.1703471550541451 1106 30-31 0.07827772532605337 1106 32-33 0.19090555219155192 1106 34-35 0.05434047671436559 1106 36-37 0.0025258845948670228 1106 38-39 0.0524790517722451 1106 40-41 0.035237586588785064 1106 42-43 -0.12493622918431413 1106 44-45 0.05349844749166266 1106 46-47 0.17328371865723824 1106 48-49 0.1029228792424064 1106 50-51 0.0772990490783414 1106 52-53 0.024445701002939302 1106 54-55 -0.008949821102881117 1106 56-57 -0.1275075135896202 1106 58-59 6.577968870473683E-4 1106 60-61 0.2889892065202915 1106 62-63 -0.18524648503278485 1106 64-65 -0.33356965441373987 1106 66-67 -0.19266043598549487 1106 68-69 -0.2596892626293368 1106 70-71 -0.3697644182814699 1106 72-73 -0.22732495352734716 1106 74-75 -0.25795431569393656 1106 76-77 -0.7299187302761254 1106 78-79 -0.7148653675755448 1106 80-81 -0.6075084585872013 1106 82-83 -0.32009591695692663 1106 84-85 -0.33522050479357546 1106 86-87 -0.3411579716468367 1106 88-89 -0.816246646949196 1106 90-91 -0.6513363958051599 1106 92-93 -0.6857781935799458 1106 94-95 -0.6520751830090425 1106 96-97 -0.5916581537621326 1106 98-99 -0.8913827803970271 1106 100-101 -0.9162948756388136 1107 1 0.04274755860396695 1107 2 -0.005465296651841811 1107 3 0.1222906454300059 1107 4 0.16122157640521806 1107 5 0.10288884331013293 1107 6 0.08190038854164783 1107 7 0.08464453474812217 1107 8 0.18331737669981862 1107 9 0.10848971381818018 1107 10-11 0.2134058367450251 1107 12-13 0.16640783656548308 1107 14-15 0.08255354699356587 1107 16-17 0.22026818753953847 1107 18-19 0.22351884326651117 1107 20-21 0.1673515910712311 1107 22-23 0.2858802884153846 1107 24-25 0.18890788947182813 1107 26-27 0.2905963244640404 1107 28-29 0.2715378486514197 1107 30-31 0.14601516399559245 1107 32-33 0.29508211086790936 1107 34-35 0.11885464207224317 1107 36-37 0.14508445543788895 1107 38-39 0.08852756421689634 1107 40-41 0.042072711644074445 1107 42-43 0.20443015462160474 1107 44-45 0.15383792097296123 1107 46-47 0.26134086707999415 1107 48-49 0.3082038934828475 1107 50-51 0.30958169879770736 1107 52-53 0.25925461781265824 1107 54-55 0.13448127572315371 1107 56-57 0.021305851073478266 1107 58-59 0.062265233243387286 1107 60-61 -0.014094868460126975 1107 62-63 -0.12713219630379058 1107 64-65 0.020546030356825895 1107 66-67 0.1618235359151079 1107 68-69 0.07207859676005768 1107 70-71 0.045730425698710775 1107 72-73 0.02004935200284308 1107 74-75 -0.14105409614204945 1107 76-77 -0.3728851562879534 1107 78-79 -0.3760990381503184 1107 80-81 -0.34076407623414084 1107 82-83 -0.4716780297684515 1107 84-85 -0.5958572052655526 1107 86-87 -0.3748161687240774 1107 88-89 -0.3945213587112555 1107 90-91 -0.6501666022488628 1107 92-93 -0.7534304087813801 1107 94-95 -0.9871626993942719 1107 96-97 -1.2752254458067256 1107 98-99 -1.390932313494167 1107 100-101 -1.3927823295268311 1108 1 -0.019075760612359716 1108 2 -0.02485414724996815 1108 3 0.02600492123617215 1108 4 0.1273602640870095 1108 5 0.05554665541954762 1108 6 0.04143933891248963 1108 7 0.11502789026119586 1108 8 0.11910377495549085 1108 9 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7444.0 3 2323.0 4 2115.0 5 2377.0 6 2796.0 7 3567.0 8 4499.0 9 5675.0 10 7125.0 11 8748.0 12 11062.0 13 13537.0 14 15730.0 15 18720.0 16 21802.0 17 27023.0 18 26985.0 19 24095.0 20 24931.0 21 26535.0 22 29065.0 23 32018.0 24 36566.0 25 40675.0 26 45835.0 27 51615.0 28 59582.0 29 69388.0 30 80066.0 31 95486.0 32 113986.0 33 141613.0 34 179680.0 35 232390.0 36 288688.0 37 291549.0 38 135685.0 39 7548.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.36129929736173 17.76029762027332 14.266599857687723 23.611803224677224 2 15.796874921425017 19.04159319667496 40.10023767932812 25.06129420257191 3 19.829108252796406 22.743814315513156 29.094203263335842 28.332874168354593 4 13.04418842246037 15.865483379642697 35.94167993876457 35.14864825913235 5 14.193571131303798 36.734168765472056 33.85056810819992 15.221691995024218 6 33.783327167570775 35.082761626735724 16.555301395904223 14.578609809789278 7 29.77365015781418 32.20677136349989 19.439018183415964 18.58056029526997 8 27.218223884555236 34.38432204552313 18.847701438487075 19.549752631434554 9 26.525472463333166 16.71304872151598 19.57228166312949 37.18919715202136 10-11 25.715884004024215 25.934727506791834 26.384146847672437 21.96524164151152 12-13 26.61464556716987 23.22513579227683 26.853257390033313 23.30696125051999 14-15 23.10675857777249 24.673075735147762 25.283820915191185 26.936344771888564 16-17 22.779355074867595 27.83825396643827 26.220633486233286 23.16175747246085 18-19 22.80959665735332 26.26534866020292 28.47156592994928 22.453488752494483 20-21 23.197352960555214 25.370458322352047 28.980442326276023 22.451746390816716 22-23 22.93596274732311 25.28685005321386 28.852304472550966 22.924882726912067 24-25 22.78229527144573 25.491486367609962 28.93508501268241 22.791133348261898 26-27 22.273019519371793 25.940057613149893 28.715922325209988 23.07100054226833 28-29 22.47159756383228 26.35844899273835 28.56290901704146 22.607044426387912 30-31 22.55756211649935 25.826060766421566 28.46282317765798 23.153553939421105 32-33 22.25732513799327 25.84791803439938 28.5824150938217 23.312341733785647 34-35 23.11622772866133 25.953082833726775 28.3071278574305 22.62356158018139 36-37 23.1253309893873 25.467844066612216 28.374033785384423 23.03279115861606 38-39 22.649688796704208 25.945021855891238 28.238273216584194 23.16701613082036 40-41 23.14785753033345 25.787817059061673 28.25205208138786 22.812273329217017 42-43 22.76050283484126 25.75171129494671 28.158371623102934 23.329414247109092 44-45 22.78028785541953 26.0377230842932 28.266029019585616 22.915960040701655 46-47 23.24147536288268 26.37917819967765 27.486827280393143 22.89251915704652 48-49 23.017000064684222 26.382476655967483 27.492188180341437 23.108335099006858 50-51 23.089462153616577 26.634697501412692 27.44804591563359 22.827794429337143 52-53 23.40330522041087 26.2438568446263 27.393770974712083 22.959066960250745 54-55 22.906599309666948 26.565570210528723 27.557592520629985 22.970237959174344 56-57 22.975786011386564 26.648207814739916 27.39818627611173 22.97781989776179 58-59 22.991404050759645 26.167482144632757 27.3521253287342 23.488988475873395 60-61 22.633957323993815 26.558891032699744 27.643183938514227 23.163967704792217 62-63 22.84519421442736 26.440066976403966 27.788304274031784 22.9264345351369 64-65 22.492358074111255 26.57776570977458 27.618219576403945 23.311656639710222 66-67 22.497131033543308 26.507490168033097 27.57857859711026 23.416800201313336 68-69 23.077923722788103 26.131856213555345 27.71650292243109 23.07371714122546 70-71 22.875672670699835 26.312253613140573 27.202160775326274 23.609912940833322 72-73 22.901081629308294 26.42388010027455 27.291007634882504 23.38403063553465 74-75 22.790908166262298 26.86355963873115 27.146933751758517 23.198598443248038 76-77 23.09602679273291 26.384173896828965 27.194665035995424 23.325134274442703 78-79 23.08243037946914 26.66825563390195 27.191335116389183 23.057978870239726 80-81 23.2575305544745 26.673808860540515 26.838644540186607 23.230016044798376 82-83 23.20927912300158 26.80656862164841 26.918953925453216 23.0651983298968 84-85 23.15194592083413 26.895207905298392 26.87498443249622 23.07786174137126 86-87 23.345019158095017 26.859587514228316 26.583213683315442 23.21217964436122 88-89 23.248406842433145 26.916892899803962 26.405459315185148 23.429240942577742 90-91 23.21431838807893 26.880141548100006 26.48144285379706 23.424097210024005 92-93 23.42374456327635 26.46339383257978 26.684612637298567 23.428248966845302 94-95 23.237763388937665 26.381968832309045 26.782630962832894 23.597636815920396 96-97 23.586224965347053 26.069577491257036 26.70456714738413 23.639630396011786 98-99 23.315409445735607 26.299264688569878 26.285526317894924 24.099799547799584 100-101 24.294491917049324 25.14549860266436 26.40186531038787 24.158144169898442 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 503.0 1 370.5 2 327.0 3 422.0 4 363.5 5 338.0 6 689.0 7 1480.5 8 2424.5 9 2832.5 10 2368.0 11 1862.0 12 1756.0 13 1826.5 14 2033.5 15 2217.0 16 2371.0 17 2635.5 18 2882.0 19 3202.0 20 3709.0 21 4303.0 22 5091.5 23 6182.0 24 7596.5 25 9547.0 26 12171.5 27 15192.0 28 17754.5 29 20191.5 30 22838.5 31 26980.0 32 32956.5 33 35312.0 34 35497.5 35 37582.5 36 41221.5 37 45112.5 38 47975.0 39 50774.5 40 54188.5 41 58642.5 42 61799.5 43 64373.0 44 67393.5 45 71154.5 46 74000.5 47 77435.0 48 80933.5 49 80888.5 50 80038.0 51 79316.0 52 79276.0 53 80560.0 54 82431.0 55 80038.5 56 75170.0 57 71291.0 58 67427.0 59 60312.0 60 49344.5 61 40903.0 62 34422.5 63 26635.0 64 19575.0 65 14941.5 66 11362.5 67 8480.0 68 6484.0 69 5115.5 70 4016.0 71 2807.5 72 1976.0 73 1520.0 74 1113.0 75 797.5 76 588.0 77 401.5 78 253.0 79 160.0 80 101.0 81 57.0 82 36.0 83 28.5 84 23.0 85 18.0 86 13.5 87 7.5 88 7.5 89 6.0 90 5.0 91 4.0 92 3.5 93 3.5 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14571464603541018 2 0.05131312245147871 3 0.15526446134472366 4 0.13072737607629617 5 0.19853563406204366 6 0.20196260127830445 7 0.1679670864929971 8 0.06872211591008369 9 0.12305096951187194 10-11 0.10319740610566758 12-13 0.1546704536939051 14-15 0.14068842745156096 16-17 0.13703299575421607 18-19 0.13481689028770075 20-21 0.13653037389583117 22-23 0.20161990455667836 24-25 0.21891466577474133 26-27 0.14891314877058692 28-29 0.12775733782220347 30-31 0.09908504544615458 32-33 0.12469591377567713 34-35 0.09383036238122132 36-37 0.0867251170195072 38-39 0.05151874048445436 40-41 0.05910376125644498 42-43 0.06234795688783856 44-45 0.04103222080269625 46-47 0.05224982682392334 48-49 0.04448203446706548 50-51 0.05519701862990765 52-53 0.028398134998748013 54-55 0.05364346015853608 56-57 0.02716442680089412 58-59 0.05284383447474188 60-61 0.03797079675616991 62-63 0.05421462136124621 64-65 0.0420831574156829 66-67 0.04064383118485335 68-69 0.06739702191979617 70-71 0.053049452507717525 72-73 0.051610126276887984 74-75 0.043705255231379685 76-77 0.021384275429467532 78-79 0.023760306032741704 80-81 0.02684457652737644 82-83 0.023554687999766052 84-85 0.02158989346244318 86-87 0.02492547488627038 88-89 0.06563784541544895 90-91 0.11021126567494804 92-93 0.08110489078483947 94-95 0.07511912138043722 96-97 0.06764833284898863 98-99 0.05572248693640097 100-101 0.134154343292557 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2188524.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.87415674306183 #Duplication Level Percentage of deduplicated Percentage of total 1 77.87538105518892 43.51221247503294 2 12.588533848554118 14.067474268389041 3 4.03972300167044 6.771483485816593 4 1.8082214123953673 4.041313864893575 5 0.972647903419001 2.7172940705721857 6 0.6179701485919729 2.071713656697666 7 0.37592248019885927 1.4703046107310134 8 0.2761258402576838 1.234263878349398 9 0.19245101768596587 0.9677734494792679 >10 1.1241795893640851 11.466055489082553 >50 0.08152338755030654 3.1075125594903237 >100 0.0416551433508001 4.296003934501322 >500 0.0027915339168635978 1.1004008285702094 >1k 0.0027094299781322867 2.4784364515918487 >5k 1.6420787746256282E-4 0.6977569768021326 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8227 0.37591545717570385 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6983 0.31907349428199094 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4162 0.19017383405436722 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3070 0.14027719138560965 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2779 0.12698055858651766 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2695 0.12314235530430555 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2347 0.10724122742085535 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2254 0.10299178807269191 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.5692896216810964E-5 2 0.0 0.0 0.0 0.0 4.5692896216810964E-5 3 0.0 0.0 0.0 0.0 4.5692896216810964E-5 4 0.0 0.0 0.0 0.0 4.5692896216810964E-5 5 0.0 0.0 0.0 0.0 4.5692896216810964E-5 6 0.0 0.0 0.0 0.0 4.5692896216810964E-5 7 0.0 0.0 0.0 0.0 4.5692896216810964E-5 8 0.0 0.0 0.0 0.0 4.5692896216810964E-5 9 0.0 0.0 0.0 0.0 4.5692896216810964E-5 10-11 0.0 0.0 0.0 0.0 4.5692896216810964E-5 12-13 0.0 0.0 0.0 0.0 6.853934432521645E-5 14-15 0.0 0.0 0.0 0.0 1.1423224054202742E-4 16-17 0.0 0.0 0.0 0.0 1.8277158486724386E-4 18-19 0.0 0.0 0.0 0.0 1.8277158486724386E-4 20-21 0.0 0.0 0.0 0.0 1.8277158486724386E-4 22-23 0.0 0.0 0.0 0.0 1.8277158486724386E-4 24-25 0.0 0.0 0.0 4.5692896216810964E-5 1.8277158486724386E-4 26-27 0.0 0.0 0.0 5.940076508185425E-4 1.8277158486724386E-4 28-29 0.0 0.0 0.0 0.002467416395707792 1.8277158486724386E-4 30-31 0.0 0.0 0.0 0.00996105137526479 1.8277158486724386E-4 32-33 0.0 0.0 0.0 0.026479033357641957 1.8277158486724386E-4 34-35 0.0 0.0 0.0 0.051221736659045086 1.8277158486724386E-4 36-37 0.0 0.0 0.0 0.09239103615039176 1.8277158486724386E-4 38-39 0.0 0.0 0.0 0.1536880564252437 1.8277158486724386E-4 40-41 0.0 0.0 0.0 0.21688133189309325 1.8277158486724386E-4 42-43 0.0 0.0 0.0 0.2806000756674361 1.8277158486724386E-4 44-45 4.5692896216810964E-5 0.0 0.0 0.361727812900384 1.8277158486724386E-4 46-47 4.5692896216810964E-5 0.0 0.0 0.4575915091632534 2.0561803297564936E-4 48-49 4.5692896216810964E-5 0.0 0.0 0.5560368540623726 2.2846448108405484E-4 50-51 4.5692896216810964E-5 0.0 0.0 0.664123400063239 2.2846448108405484E-4 52-53 4.5692896216810964E-5 0.0 0.0 0.7610608793872035 2.741573773008658E-4 54-55 4.5692896216810964E-5 0.0 0.0 0.868484878392926 3.1985027351767676E-4 56-57 4.5692896216810964E-5 0.0 0.0 0.990187907466402 3.426967216260822E-4 58-59 4.5692896216810964E-5 0.0 0.0 1.119270339278893 3.655431697344877E-4 60-61 4.5692896216810964E-5 4.5692896216810964E-5 0.0 1.2559834847595914 3.655431697344877E-4 62-63 4.5692896216810964E-5 4.5692896216810964E-5 0.0 1.3893382023683543 3.655431697344877E-4 64-65 4.5692896216810964E-5 4.5692896216810964E-5 0.0 1.5300951691642404 3.655431697344877E-4 66-67 4.5692896216810964E-5 4.5692896216810964E-5 0.0 1.6885124403479241 3.655431697344877E-4 68-69 4.5692896216810964E-5 4.5692896216810964E-5 0.0 1.8582387033452683 3.655431697344877E-4 70-71 4.5692896216810964E-5 4.5692896216810964E-5 0.0 2.036098301869205 3.655431697344877E-4 72-73 4.5692896216810964E-5 4.5692896216810964E-5 0.0 2.21870996159969 3.883896178428932E-4 74-75 4.5692896216810964E-5 4.5692896216810964E-5 0.0 2.3981459650431067 4.1123606595129867E-4 76-77 4.5692896216810964E-5 6.853934432521645E-5 0.0 2.59611043790244 4.1123606595129867E-4 78-79 4.5692896216810964E-5 9.138579243362193E-5 0.0 2.8165101228042277 4.1123606595129867E-4 80-81 4.5692896216810964E-5 9.138579243362193E-5 0.0 3.043375352520694 4.1123606595129867E-4 82-83 4.5692896216810964E-5 9.138579243362193E-5 0.0 3.2828975144892176 4.1123606595129867E-4 84-85 4.5692896216810964E-5 9.138579243362193E-5 0.0 3.526235033291844 4.1123606595129867E-4 86-87 4.5692896216810964E-5 9.138579243362193E-5 0.0 3.7762665613902335 4.3408251405970417E-4 88-89 4.5692896216810964E-5 9.138579243362193E-5 0.0 4.0421078315796395 4.5692896216810967E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4565 0.0 51.018612 1 GTATCAA 7640 0.0 39.21189 1 TATCAAC 9750 0.0 30.487274 2 ATCAACG 9810 0.0 30.300808 3 TCAACGC 9950 0.0 29.778868 4 CAACGCA 10130 0.0 29.296679 5 AACGCAG 10500 0.0 28.395 6 CGCAGAG 11570 0.0 25.637497 8 ACGCAGA 11620 0.0 25.610758 7 TACATGG 7355 0.0 23.860905 2 GTACATG 7590 0.0 23.845276 1 GAGTACT 7440 0.0 22.744772 12-13 GCAGAGT 12740 0.0 22.647682 9 ACATGGG 7415 0.0 22.449162 3 TAAGGTG 1285 0.0 21.836956 5 AGAGTAC 10730 0.0 21.773005 10-11 CAGAGTA 11925 0.0 21.604063 10-11 GTACTTT 7830 0.0 21.427313 14-15 GTGTAAG 915 0.0 21.34142 1 GTAAGGT 1195 0.0 21.093622 4 >>END_MODULE