FastQCFastQC Report
Thu 26 May 2016
SRR937976_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937976_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2188524
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83900.383363399259044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71050.3246480276204419No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42720.19520005263821644No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30910.1412367422061627No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28800.13159554110441557No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27550.1258839290773142No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25400.11605995639069985No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24280.11094235201441702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48950.045.614121
GTATCAA79700.037.909921
TATCAAC99900.030.192072
TCAACGC100300.029.9714684
ATCAACG100100.029.9378323
CAACGCA102700.029.2248235
AACGCAG105500.028.4491866
ACGCAGA117950.025.285237
CGCAGAG118150.025.1218458
TACATGG65700.022.4120182
GAGTACT80950.022.41102412-13
GTACATG69100.022.2751711
GCAGAGT132850.022.0918549
TATGTCG1100.002881589621.5865155
GTGTAGC16250.021.3414341
GTACTTT83600.021.2740814-15
ATAACGC1353.801507E-421.107783
AGAGTAC111600.021.04346510-11
GTAAGGT12000.020.9748974
ACATGGG67800.020.3839533
Produced by FastQC (version 0.11.5)