##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937976_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2188524 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52830629227735 34.0 31.0 34.0 31.0 34.0 2 32.74532835829079 34.0 31.0 34.0 31.0 34.0 3 32.801316320954214 34.0 31.0 34.0 31.0 34.0 4 36.25053552074366 37.0 35.0 37.0 35.0 37.0 5 36.22157947548211 37.0 37.0 37.0 35.0 37.0 6 36.25470773909722 37.0 37.0 37.0 35.0 37.0 7 36.21365998270981 37.0 37.0 37.0 35.0 37.0 8 36.19788496721991 37.0 37.0 37.0 35.0 37.0 9 37.89440737227465 39.0 38.0 39.0 35.0 39.0 10-11 37.870838291012575 39.0 38.0 39.0 35.0 39.0 12-13 37.78843869201343 39.0 38.0 39.0 35.0 39.0 14-15 39.0823820072341 40.0 38.5 41.0 36.0 41.0 16-17 38.966182915974414 40.0 38.0 41.0 36.0 41.0 18-19 39.21236276138621 40.5 39.0 41.0 36.0 41.0 20-21 39.18706488939577 40.5 39.0 41.0 36.0 41.0 22-23 39.09394368076384 40.0 39.0 41.0 35.5 41.0 24-25 38.99133959691555 40.0 38.5 41.0 35.0 41.0 26-27 38.81569838850294 40.0 38.0 41.0 35.0 41.0 28-29 38.701844256677106 40.0 38.0 41.0 34.5 41.0 30-31 38.46621170249904 40.0 38.0 41.0 34.0 41.0 32-33 38.25688226402818 40.0 38.0 41.0 34.0 41.0 34-35 38.103196949176706 40.0 38.0 41.0 34.0 41.0 36-37 38.10131051795639 40.0 38.0 41.0 33.5 41.0 38-39 37.95107867220099 40.0 38.0 41.0 33.0 41.0 40-41 37.777819206003684 40.0 37.0 41.0 33.0 41.0 42-43 37.54095934063323 40.0 37.0 41.0 33.0 41.0 44-45 37.27293006610848 40.0 37.0 41.0 32.0 41.0 46-47 36.88658017915271 40.0 36.0 41.0 31.0 41.0 48-49 36.483285081634925 39.0 36.0 41.0 30.0 41.0 50-51 36.09217171024855 39.0 35.0 40.5 29.5 41.0 52-53 35.56063881410485 38.5 35.0 40.0 28.0 41.0 54-55 35.23489438543969 38.0 34.5 40.0 27.0 41.0 56-57 34.86989724581498 38.0 34.0 40.0 26.0 41.0 58-59 34.406863484247836 37.5 34.0 40.0 25.5 41.0 60-61 33.90201638181715 37.0 33.0 40.0 24.5 41.0 62-63 33.415497156987996 36.0 33.0 39.0 23.0 40.5 64-65 32.9010616287507 36.0 32.0 39.0 22.0 40.0 66-67 32.328937448252795 35.0 31.0 38.5 20.0 40.0 68-69 31.72189795496874 35.0 31.0 38.0 18.0 40.0 70-71 31.080003920450494 35.0 30.0 37.0 13.5 39.5 72-73 30.48096091246886 34.0 29.5 36.5 8.0 39.0 74-75 29.874956134819634 34.0 29.0 36.0 7.0 39.0 76-77 28.531168723760857 32.5 27.0 34.5 4.5 36.5 78-79 29.208125202191066 34.0 29.0 35.0 2.0 37.0 80-81 28.876586457356648 34.0 29.0 35.0 2.0 37.0 82-83 28.302228351162704 33.5 27.5 35.0 2.0 36.0 84-85 27.697269712372357 33.0 26.0 35.0 2.0 36.0 86-87 27.045415768801256 32.5 25.5 35.0 2.0 35.0 88-89 26.272893054862546 32.0 24.5 34.5 2.0 35.0 90-91 25.704906823046038 32.0 22.5 34.0 2.0 35.0 92-93 24.86442003834548 31.0 19.0 34.0 2.0 35.0 94-95 24.006177908032996 31.0 11.0 34.0 2.0 35.0 96-97 22.919245802193622 30.5 2.0 34.0 2.0 35.0 98-99 21.43459998610936 30.0 2.0 34.0 2.0 35.0 100-101 17.058692982119457 22.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.020755222081085378 1101 2 0.05442234740208818 1101 3 0.011096816837095957 1101 4 0.03694219456148318 1101 5 0.010063471218160203 1101 6 0.04648356026569189 1101 7 0.058061760911499505 1101 8 -0.043237910013978365 1101 9 -0.16138693805373805 1101 10-11 -0.02735293261665106 1101 12-13 -0.0189043634347712 1101 14-15 0.046812742924011275 1101 16-17 0.09012548024558242 1101 18-19 0.19264701818766383 1101 20-21 0.07876668501809547 1101 22-23 0.0797669483000476 1101 24-25 0.12979167217238796 1101 26-27 0.1778418903801935 1101 28-29 0.09970606182289288 1101 30-31 0.1798367794441731 1101 32-33 0.3059434946269093 1101 34-35 0.13831085147209876 1101 36-37 0.11829006560739685 1101 38-39 0.11642134774099588 1101 40-41 0.25151883345018433 1101 42-43 0.26207026677545997 1101 44-45 0.2599329308306224 1101 46-47 0.2257484662018996 1101 48-49 0.27047663810612477 1101 50-51 0.10564558691340409 1101 52-53 -0.021061054713761962 1101 54-55 -0.013506175218367389 1101 56-57 0.07350714775593303 1101 58-59 0.24229066778296726 1101 60-61 0.2737353429191316 1101 62-63 0.2326036930975377 1101 64-65 0.17670745553546396 1101 66-67 -0.14653164928592588 1101 68-69 0.12623578567352212 1101 70-71 -0.014841049713968602 1101 72-73 -0.08238223396094213 1101 74-75 -0.250972977076664 1101 76-77 -0.4097480502076003 1101 78-79 -0.3437612663179017 1101 80-81 -0.32730969739682436 1101 82-83 -0.27251637547414376 1101 84-85 -0.3492788314671529 1101 86-87 -0.5153228868627977 1101 88-89 -0.4693018740318635 1101 90-91 -0.5233987513402276 1101 92-93 -0.5104641798328124 1101 94-95 -0.6323204285922763 1101 96-97 -0.5674453713397085 1101 98-99 -0.4205333767681374 1101 100-101 -0.7563910858782172 1102 1 0.1354747295099088 1102 2 0.16308987711680345 1102 3 0.12120398580867686 1102 4 0.06087358576284885 1102 5 0.1154071313947469 1102 6 0.05781429350051326 1102 7 0.10331896761698545 1102 8 0.15661091964827278 1102 9 0.15799960144831715 1102 10-11 0.13696194768531456 1102 12-13 0.11957435593651411 1102 14-15 0.1440234858769287 1102 16-17 0.1455152074699697 1102 18-19 0.195164332942376 1102 20-21 0.11071045622507825 1102 22-23 0.1856537888517238 1102 24-25 0.2064330381513102 1102 26-27 0.18777723219768916 1102 28-29 0.14880027216327818 1102 30-31 0.2547501323154151 1102 32-33 0.1952270669416123 1102 34-35 0.19909541144083676 1102 36-37 0.1016181369297442 1102 38-39 0.09891633904987884 1102 40-41 0.038892823936315324 1102 42-43 0.09202816677534287 1102 44-45 -0.1604539384762802 1102 46-47 -0.043618062009329606 1102 48-49 0.031247818091543422 1102 50-51 -0.019980719542196823 1102 52-53 0.08881489875676607 1102 54-55 0.1199295814959811 1102 56-57 0.05928035234409634 1102 58-59 0.10510663784936014 1102 60-61 0.043532025856954704 1102 62-63 0.18257452708128596 1102 64-65 0.1541254473877629 1102 66-67 0.09443433856176142 1102 68-69 0.030227715430957147 1102 70-71 -0.23140570378858172 1102 72-73 -0.38933806475814237 1102 74-75 -0.2864853306744166 1102 76-77 -0.37746614100134934 1102 78-79 -0.31825068498110554 1102 80-81 -0.29882652976793267 1102 82-83 -0.15418485939292736 1102 84-85 -0.41116501006176165 1102 86-87 -0.3710944130846343 1102 88-89 -0.535013554170451 1102 90-91 -0.7052364803110578 1102 92-93 -0.6580977419951566 1102 94-95 -0.8879691875988236 1102 96-97 -0.8039453789686455 1102 98-99 -0.7491761652505531 1102 100-101 -1.0727200644573323 1103 1 0.1875579515873227 1103 2 0.1443084390351217 1103 3 0.1836094088103053 1103 4 0.12198347858221581 1103 5 0.09836867112362313 1103 6 0.0744191681572346 1103 7 0.13103617798895328 1103 8 0.1784356378914751 1103 9 0.2980822963147105 1103 10-11 0.16835225086573757 1103 12-13 0.27653950660638316 1103 14-15 0.3202366109316088 1103 16-17 0.34254816838477353 1103 18-19 0.2833277638135385 1103 20-21 0.30437540225980797 1103 22-23 0.3640537034071798 1103 24-25 0.35526811768178135 1103 26-27 0.4430039499795839 1103 28-29 0.3676105705033521 1103 30-31 0.387329273620459 1103 32-33 0.3146716092281352 1103 34-35 0.32469468788916345 1103 36-37 0.29518536712893706 1103 38-39 0.3902239473639071 1103 40-41 0.33506077246051547 1103 42-43 0.2730248228485479 1103 44-45 0.2736062794754801 1103 46-47 0.18270578872177623 1103 48-49 0.1603145296014432 1103 50-51 -0.10304656524434819 1103 52-53 -0.03073224515707551 1103 54-55 -0.014433214676742523 1103 56-57 0.03673412764085526 1103 58-59 0.23023494669502753 1103 60-61 0.17914365313239955 1103 62-63 0.10681873486151261 1103 64-65 -0.09639568479790483 1103 66-67 0.04292025441479552 1103 68-69 0.15053113446259303 1103 70-71 0.036775861323683046 1103 72-73 -0.19259756961155716 1103 74-75 -0.23563855478568385 1103 76-77 -0.0955702719951077 1103 78-79 -0.15108275122789294 1103 80-81 -0.08080420738066607 1103 82-83 -0.21805567905835588 1103 84-85 -0.5182270243941716 1103 86-87 -0.5684523359641105 1103 88-89 -0.7788359249611467 1103 90-91 -0.7185310606045014 1103 92-93 -0.7501884404702253 1103 94-95 -0.7923858297418427 1103 96-97 -1.1754536001654827 1103 98-99 -1.1619740991287486 1103 100-101 -1.4966339699888458 1104 1 0.1179854476323996 1104 2 0.11274390506617493 1104 3 0.12333336732084632 1104 4 0.09357283022216478 1104 5 0.006302772268362844 1104 6 0.026359378069621187 1104 7 0.006373922354100614 1104 8 0.09206038520508741 1104 9 0.11615901026505782 1104 10-11 0.04454164491698265 1104 12-13 0.13239801121652306 1104 14-15 0.1558469302724177 1104 16-17 0.0728824965219772 1104 18-19 0.0583043701949606 1104 20-21 0.13218788440565277 1104 22-23 0.11586415443272813 1104 24-25 0.06512562042663461 1104 26-27 0.15370657594938564 1104 28-29 -0.01717930847465965 1104 30-31 -0.009974078220203353 1104 32-33 0.06840929213756652 1104 34-35 0.07552360195235508 1104 36-37 -0.03482877924311367 1104 38-39 0.09630744369827937 1104 40-41 0.07768111024018509 1104 42-43 0.05828509918961089 1104 44-45 0.09717527797182868 1104 46-47 0.1777675044364031 1104 48-49 0.12752708659831313 1104 50-51 0.2284495157762123 1104 52-53 0.08226765234538647 1104 54-55 0.007202646023330317 1104 56-57 -0.02092587283418368 1104 58-59 0.08701186347922629 1104 60-61 -0.07831218679854146 1104 62-63 -0.08461692182780212 1104 64-65 -0.3335844020230354 1104 66-67 -0.3831209247733689 1104 68-69 -0.23203290028556722 1104 70-71 -0.2498573256671328 1104 72-73 -0.47665783302808507 1104 74-75 -0.4687776563551793 1104 76-77 -0.5903047017311174 1104 78-79 -0.4709123014605687 1104 80-81 -0.5527300462395566 1104 82-83 -0.8199182240829117 1104 84-85 -0.7146104875826893 1104 86-87 -0.9158851792137099 1104 88-89 -0.9752983476306021 1104 90-91 -0.9192807263105998 1104 92-93 -0.9154413258615008 1104 94-95 -0.9380453158033539 1104 96-97 -1.3703243152495048 1104 98-99 -1.2862781483378392 1104 100-101 -1.5260021892899225 1105 1 0.16060484316064816 1105 2 0.16232465153697007 1105 3 0.21219630385033383 1105 4 0.13410906204316575 1105 5 0.11360341681641017 1105 6 0.11075413044454052 1105 7 0.06736348623223165 1105 8 0.05192814833476689 1105 9 0.1901764790067233 1105 10-11 0.17001450028907072 1105 12-13 0.15358102030765508 1105 14-15 0.19450593222257595 1105 16-17 0.18426690849074845 1105 18-19 0.21771725572785527 1105 20-21 0.2936727460933213 1105 22-23 0.22801159891604783 1105 24-25 0.22417832892273282 1105 26-27 0.184037791727647 1105 28-29 0.16203616647941743 1105 30-31 0.0930712564357492 1105 32-33 0.14468488547666425 1105 34-35 0.08782835585417104 1105 36-37 0.1539557822659532 1105 38-39 0.14836084644416303 1105 40-41 0.14405996676045163 1105 42-43 0.16145753687235498 1105 44-45 0.10054034873413542 1105 46-47 0.18545324940205887 1105 48-49 0.12848735151955992 1105 50-51 0.26586172715548173 1105 52-53 0.06466144880609903 1105 54-55 0.24869000626438265 1105 56-57 0.12343675054742675 1105 58-59 0.047303614130228766 1105 60-61 0.06677503778308136 1105 62-63 0.14473194397191946 1105 64-65 -0.013931995135067154 1105 66-67 -0.005896129759371149 1105 68-69 -0.02833481337776078 1105 70-71 -0.09694679065972878 1105 72-73 -0.08651742302915011 1105 74-75 -0.1453290435806096 1105 76-77 -0.16659137903424792 1105 78-79 -0.22546268304934003 1105 80-81 -0.40299159671416973 1105 82-83 -0.22618922016664555 1105 84-85 -0.26334160159565556 1105 86-87 -0.28433965820213913 1105 88-89 -0.6412367875015512 1105 90-91 -0.7322351040931174 1105 92-93 -0.8012720420326715 1105 94-95 -0.5723992232528765 1105 96-97 -1.1295551337246295 1105 98-99 -1.2706777279344728 1105 100-101 -1.6219678749946969 1106 1 0.04918890709563328 1106 2 0.04418372627488054 1106 3 0.1855845056753722 1106 4 0.11292976627576934 1106 5 0.14740855251303486 1106 6 0.14046828338019424 1106 7 0.10351205433995858 1106 8 0.13507531381293347 1106 9 0.07703859595564211 1106 10-11 0.08895183509000759 1106 12-13 0.04290927258097099 1106 14-15 0.19122701086813976 1106 16-17 0.25941809514627323 1106 18-19 0.32659640567685955 1106 20-21 0.32958339615065313 1106 22-23 0.38958851009242323 1106 24-25 0.28366605392135114 1106 26-27 0.22967615001097386 1106 28-29 0.2519981744691151 1106 30-31 0.2655131724272479 1106 32-33 0.29891399355449977 1106 34-35 0.3196874668232539 1106 36-37 0.24902131359251456 1106 38-39 0.2724307619844595 1106 40-41 0.2380241911319061 1106 42-43 0.18310228247032967 1106 44-45 0.18550366225384352 1106 46-47 0.08168209130153059 1106 48-49 0.017251605165803596 1106 50-51 0.009190178941814509 1106 52-53 -0.0145963000157181 1106 54-55 -0.0170044330473047 1106 56-57 -0.2354092478762837 1106 58-59 0.02083239291498984 1106 60-61 -0.22883970985107993 1106 62-63 -0.04717794861231539 1106 64-65 -0.002719412579317293 1106 66-67 -0.06073877655782667 1106 68-69 -0.16889631446916553 1106 70-71 -0.5710627560236894 1106 72-73 -0.4962020403107914 1106 74-75 -0.11365031555572358 1106 76-77 -0.4787299579654345 1106 78-79 -0.3556277691913756 1106 80-81 -0.4670834586849857 1106 82-83 -0.26601283714418855 1106 84-85 -0.5298523292843171 1106 86-87 -0.8195194046853587 1106 88-89 -1.035644046683192 1106 90-91 -1.0627199424332368 1106 92-93 -0.9072952092122506 1106 94-95 -0.9445285366648655 1106 96-97 -1.154420951567321 1106 98-99 -1.0800710707217327 1106 100-101 -1.45896418013945 1107 1 0.04498252020543703 1107 2 0.045419466562449884 1107 3 0.042380671430962025 1107 4 -0.01216611012585389 1107 5 0.0038557078754166696 1107 6 0.07540625287019509 1107 7 0.08468578440882624 1107 8 0.050042583942918384 1107 9 0.040329601633146694 1107 10-11 0.0669043209774074 1107 12-13 0.049007823376186366 1107 14-15 0.021442585659848135 1107 16-17 0.04295352984683376 1107 18-19 0.02777959149242548 1107 20-21 0.048411898124996355 1107 22-23 0.06547858805160445 1107 24-25 0.010093648468675553 1107 26-27 0.101264674873903 1107 28-29 0.1175104689689519 1107 30-31 0.009213737742918227 1107 32-33 0.03894183322074696 1107 34-35 0.029624320291645745 1107 36-37 0.05083413850698548 1107 38-39 -0.05673676706253872 1107 40-41 0.026656800877411513 1107 42-43 0.028843746934178682 1107 44-45 0.0983190398141005 1107 46-47 0.06939672254770102 1107 48-49 0.0644298967193464 1107 50-51 -0.043931434947175774 1107 52-53 -0.025156908105458342 1107 54-55 -0.0038100139669978716 1107 56-57 0.010295083505027947 1107 58-59 0.22022081307481045 1107 60-61 0.004841010571297488 1107 62-63 -0.048040328944360056 1107 64-65 -0.07793521057534036 1107 66-67 -0.2636954221621224 1107 68-69 -0.15858528939317296 1107 70-71 -0.23510677191583795 1107 72-73 -0.3240739697295254 1107 74-75 -0.4743058869384953 1107 76-77 -0.6556488049324933 1107 78-79 -0.4307187756805284 1107 80-81 -0.2918487897781574 1107 82-83 -0.28394527522528534 1107 84-85 -0.5164783321819151 1107 86-87 -0.5360225234584668 1107 88-89 -0.7148304466409847 1107 90-91 -0.9120752877709002 1107 92-93 -0.9018287324721719 1107 94-95 -0.8816488356774883 1107 96-97 -1.0770705086082017 1107 98-99 -1.3820411532261652 1107 100-101 -1.74371202013036 1108 1 0.003995875732961451 1108 2 0.01173877102888099 1108 3 -0.06475036821370139 1108 4 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6381.0 14 8547.0 15 12107.0 16 15628.0 17 21584.0 18 22544.0 19 18684.0 20 17744.0 21 18659.0 22 20364.0 23 21873.0 24 24716.0 25 28169.0 26 32533.0 27 38266.0 28 45704.0 29 54827.0 30 65616.0 31 80820.0 32 100082.0 33 128648.0 34 173134.0 35 243783.0 36 345082.0 37 412655.0 38 210228.0 39 9832.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.56335742654024 18.255208250030734 14.683680123492726 23.497754199936296 2 16.026161879370555 19.314219489613027 39.64465214224151 25.014966488774903 3 19.90294833614295 22.75632574592644 29.261638728174454 28.07908718975616 4 13.249581224223473 16.19110979921609 35.76738090821111 34.79192806834933 5 14.082072427491632 36.79848261782239 33.947725069065385 15.171719885620602 6 33.7652902229391 35.33308354162002 16.373298484343085 14.52832775109779 7 29.751787049171043 32.17931354648156 19.362136307392564 18.70676309695484 8 27.16725976045956 34.70398314114902 18.68126646086586 19.447490637525565 9 26.239191345399917 16.741922866735752 19.478333342471913 37.54055244539242 10-11 25.676391942697453 26.05479766271697 26.358198493596596 21.91061190098898 12-13 26.55469538029913 23.19626503450411 26.9261357340562 23.322903851140563 14-15 22.99282530143604 24.7069943030097 25.258393328106067 27.041787067448197 16-17 22.720076948823777 27.91156368554708 26.243044504887553 23.125314860741593 18-19 22.772060073364514 26.268914574388948 28.514583344756556 22.44444200748998 20-21 23.08357139332262 25.347448782832632 29.041628056169362 22.527351767675384 22-23 22.904683704633808 25.336916570254658 28.896941500298833 22.861458224812704 24-25 22.778354165148272 25.56184441013791 28.888370000632875 22.771431424080948 26-27 22.30298814622324 25.979203675918466 28.73618822720752 22.981619950650774 28-29 22.504039525200422 26.351161774759003 28.602717266137077 22.542081433903498 30-31 22.528882274373725 25.863498096600658 28.48585279190369 23.12176683712193 32-33 22.319860325954842 25.77936088432204 28.56123579179392 23.339542997929197 34-35 23.052891596298615 25.9848042935308 28.319271147144914 22.643032963025668 36-37 23.155232504343715 25.483851437566468 28.38982731382004 22.97108874426978 38-39 22.61131551627077 25.966454097485723 28.28824234714393 23.13398803909957 40-41 23.11211261890684 25.74366751687703 28.32018416451982 22.824035699696317 42-43 22.758717750884585 25.69556602008315 28.2397393773634 23.305976851668863 44-45 22.778754082660278 26.029369565972317 28.288540587171994 22.90333576419541 46-47 23.17436680425575 26.357154818903993 27.621958477446988 22.846519899393268 48-49 23.01287702886371 26.30269596727819 27.54950344058365 23.134923563274455 50-51 23.066527449027152 26.573075552224896 27.524378318604292 22.836018680143667 52-53 23.42328715573293 26.22602248796055 27.451936445498422 22.8987539108081 54-55 22.954769419108935 26.515871407430293 27.566410088680826 22.96294908477995 56-57 22.957499145961226 26.593110791965298 27.443836239350222 23.005553822723257 58-59 22.99369579629106 26.196894904877066 27.33826496485693 23.471144333974944 60-61 22.591573597705388 26.573376085423757 27.619810722842075 23.21523959402878 62-63 22.788857986330814 26.534915282278533 27.779282154218315 22.89694457717234 64-65 22.441170428372654 26.654902694721955 27.53928008056538 23.364646796340004 66-67 22.445183756660665 26.57877178711464 27.521387246254946 23.45465720996975 68-69 22.96309670047136 26.181951149835736 27.66738465933438 23.18756749035852 70-71 22.819529904686693 26.323239884614964 27.210266928440074 23.64696328225827 72-73 22.84947059568096 26.450109966034592 27.239292762804656 23.46112667547979 74-75 22.68862764883725 26.87336605955661 27.096088094368152 23.34191819723799 76-77 22.8928192420438 26.40388072989458 27.337455131117228 23.36584489694439 78-79 22.852780609604014 26.643260965794813 27.373449360890906 23.130509063710264 80-81 23.0579090236078 26.627046146253186 27.02256587425626 23.292478955882757 82-83 22.938852003793894 26.72966209189912 27.145185066678856 23.186300837628128 84-85 22.88668369124948 26.859467210539243 27.101627618672197 23.15222147953908 86-87 23.027674694020643 26.85417067562052 26.808440034353975 23.309714596004866 88-89 22.85396581427442 27.021229482291854 26.596895775789832 23.527908927643892 90-91 22.837809117023973 26.861832317512118 26.794118520854614 23.506240044609292 92-93 22.918012564583105 26.54028804253064 26.953866713772616 23.58783267911364 94-95 22.744906637491884 26.418786111450054 27.06859227553719 23.767714975520875 96-97 22.956545662106716 26.122598577266427 27.041831986553223 23.879023774073637 98-99 22.647016955712974 26.408545238807374 26.680519380813838 24.26391842466581 100-101 23.125544092949927 25.648170362500146 26.821766002901832 24.4045195416481 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 468.0 1 321.0 2 284.5 3 389.0 4 326.0 5 325.5 6 656.5 7 1472.0 8 2484.5 9 2916.5 10 2415.5 11 1853.0 12 1772.5 13 1848.5 14 2001.5 15 2182.0 16 2352.0 17 2487.5 18 2714.0 19 3119.0 20 3545.5 21 4160.0 22 4956.0 23 5942.5 24 7403.5 25 9390.5 26 11971.5 27 15008.0 28 17561.5 29 19772.0 30 22431.0 31 26840.0 32 33124.0 33 35208.5 34 35356.0 35 37916.0 36 41349.5 37 45245.0 38 48140.5 39 50617.0 40 54342.0 41 59338.5 42 62465.5 43 65117.0 44 68565.5 45 72328.5 46 74490.5 47 78117.0 48 82461.5 49 82100.0 50 81127.0 51 79886.5 52 79383.5 53 81407.5 54 84203.5 55 81500.5 56 75774.5 57 71528.0 58 67334.0 59 60207.0 60 48635.5 61 39664.5 62 33451.5 63 25216.0 64 17904.5 65 13764.5 66 10179.5 67 7596.5 68 5951.5 69 4632.5 70 3519.5 71 2403.5 72 1646.5 73 1252.5 74 967.5 75 704.0 76 473.5 77 297.5 78 200.5 79 124.0 80 68.0 81 41.5 82 25.0 83 14.5 84 8.5 85 4.5 86 1.5 87 1.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00991535847904798 2 0.013799254657476912 3 0.009367043724446248 4 9.138579243362193E-5 5 8.224721319025974E-4 6 0.0015535584713715729 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.539327875773809E-4 14-15 0.0 16-17 0.0025816486362498195 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003861049730320527 26-27 0.02033333881648088 28-29 0.006762548640088023 30-31 0.0032441956313935785 32-33 0.0 34-35 0.009526968861205085 36-37 7.539327875773809E-4 38-39 0.0027415737730086577 40-41 0.0021018732259733045 42-43 0.003312734975718795 44-45 0.0 46-47 1.1423224054202742E-4 48-49 6.62546995143759E-4 50-51 0.00475206120654834 52-53 0.009024347002820166 54-55 0.00779063880496627 56-57 0.017888768868881494 58-59 0.024651317508969515 60-61 0.00424943934816342 62-63 0.021178657396491885 64-65 0.022275286905695347 66-67 0.013890640449910533 68-69 0.032510495658261 70-71 0.01699775739265368 72-73 0.005528840442234127 74-75 0.016929218048328462 76-77 0.022023975976502887 78-79 0.02721011969711093 80-81 0.012062924601238095 82-83 0.03650862407723196 84-85 0.02337191641489881 86-87 0.03257903500258622 88-89 0.023097759037597945 90-91 0.029197760682542206 92-93 0.025062553574920817 94-95 0.01521573444019805 96-97 0.01562697050614935 98-99 0.012085771049346501 100-101 0.01137753115798593 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2188524.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.28859361251398 #Duplication Level Percentage of deduplicated Percentage of total 1 75.86426810835093 40.427001529367175 2 13.480093692840203 14.366704693127488 3 4.480958873936286 7.16351989282737 4 2.0134489788778622 4.291754575798145 5 1.1237650025941264 2.994192826960206 6 0.6672348570644264 2.133360428532604 7 0.44625377206215017 1.6646165135229958 8 0.30376234503869737 1.294965452764112 9 0.2168496216607971 1.0400050227338662 >10 1.2583154160829184 12.213462938920898 >50 0.09376166560718238 3.4361629762502797 >100 0.045105477337847484 4.5277722194969865 >500 0.003005230543459664 1.0898977736486979 >1k 0.003005230543459618 2.647600536289149 >5k 1.7172745962626388E-4 0.7089826197601334 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8390 0.383363399259044 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7105 0.3246480276204419 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4272 0.19520005263821644 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3091 0.1412367422061627 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2880 0.13159554110441557 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2755 0.1258839290773142 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2540 0.11605995639069985 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2428 0.11094235201441702 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.370786886504329E-4 14-15 0.0 0.0 0.0 0.0 1.8277158486724386E-4 16-17 0.0 0.0 0.0 0.0 1.8277158486724386E-4 18-19 0.0 0.0 0.0 0.0 1.8277158486724386E-4 20-21 0.0 0.0 0.0 0.0 1.8277158486724386E-4 22-23 0.0 0.0 0.0 0.0 2.2846448108405484E-4 24-25 0.0 0.0 0.0 4.5692896216810964E-5 2.2846448108405484E-4 26-27 0.0 0.0 0.0 6.397005470353535E-4 2.513109291924603E-4 28-29 0.0 0.0 0.0 0.0025359557400330087 2.741573773008658E-4 30-31 0.0 0.0 0.0 0.010029590719590007 2.741573773008658E-4 32-33 0.0 0.0 0.0 0.02663895849440079 2.741573773008658E-4 34-35 0.0 0.0 0.0 0.05142735469202074 3.1985027351767676E-4 36-37 0.0 0.0 0.0 0.09277942576823467 3.1985027351767676E-4 38-39 0.0 0.0 0.0 0.15439629631660426 3.426967216260822E-4 40-41 0.0 0.0 0.0 0.2183435045720312 3.655431697344877E-4 42-43 0.0 0.0 0.0 0.28233640572367497 3.655431697344877E-4 44-45 0.0 0.0 0.0 0.3638068396782489 3.655431697344877E-4 46-47 0.0 0.0 0.0 0.4600360791108528 3.655431697344877E-4 48-49 0.0 0.0 0.0 0.5585728098024056 4.1123606595129867E-4 50-51 0.0 0.0 0.0 0.6664308913221879 4.1123606595129867E-4 52-53 0.0 0.0 0.0 0.7637567602639953 4.5692896216810967E-4 54-55 0.0 0.0 0.0 0.8718661527129701 4.5692896216810967E-4 56-57 0.0 0.0 0.0 0.9946658112956495 4.5692896216810967E-4 58-59 0.0 0.0 0.0 1.1242965578627422 4.5692896216810967E-4 60-61 0.0 0.0 0.0 1.2621977186450777 4.5692896216810967E-4 62-63 0.0 0.0 0.0 1.3958951329754665 5.026218583849206E-4 64-65 0.0 0.0 0.0 1.5375888041437973 5.026218583849206E-4 66-67 0.0 0.0 0.0 1.6978109447280452 5.026218583849206E-4 68-69 0.0 0.0 0.0 1.8690907661967608 5.026218583849206E-4 70-71 0.0 0.0 0.0 2.0481155335742263 5.026218583849206E-4 72-73 0.0 0.0 0.0 2.232737680738251 5.026218583849206E-4 74-75 0.0 0.0 0.0 2.414069939374665 5.026218583849206E-4 76-77 0.0 0.0 0.0 2.6139535138751047 5.026218583849206E-4 78-79 0.0 0.0 0.0 2.8363865326585405 5.483147546017316E-4 80-81 0.0 0.0 0.0 3.0670899656572193 5.483147546017316E-4 82-83 0.0 0.0 0.0 3.3108387205257976 5.483147546017316E-4 84-85 0.0 0.0 0.0 3.5578545174738774 5.483147546017316E-4 86-87 0.0 0.0 0.0 3.8139860472172114 5.71161202710137E-4 88-89 0.0 0.0 0.0 4.086018704843996 5.940076508185425E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4895 0.0 45.61412 1 GTATCAA 7970 0.0 37.90992 1 TATCAAC 9990 0.0 30.19207 2 TCAACGC 10030 0.0 29.971468 4 ATCAACG 10010 0.0 29.937832 3 CAACGCA 10270 0.0 29.224823 5 AACGCAG 10550 0.0 28.449186 6 ACGCAGA 11795 0.0 25.28523 7 CGCAGAG 11815 0.0 25.121845 8 TACATGG 6570 0.0 22.412018 2 GAGTACT 8095 0.0 22.411024 12-13 GTACATG 6910 0.0 22.275171 1 GCAGAGT 13285 0.0 22.091854 9 TATGTCG 110 0.0028815896 21.586515 5 GTGTAGC 1625 0.0 21.341434 1 GTACTTT 8360 0.0 21.27408 14-15 ATAACGC 135 3.801507E-4 21.10778 3 AGAGTAC 11160 0.0 21.043465 10-11 GTAAGGT 1200 0.0 20.974897 4 ACATGGG 6780 0.0 20.383953 3 >>END_MODULE