FastQCFastQC Report
Thu 26 May 2016
SRR937975_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937975_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612174
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102910.6383306020317906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84070.5214697669110158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52960.32850052165585103No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21060.130631060915261No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17570.10898327351762278No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA16640.1032146654145272No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16150.10017529125268115No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA16150.10017529125268115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41850.055.152041
GTATCAA70500.040.518131
TATCAAC89150.031.6629072
ATCAACG90800.031.1922073
TCAACGC93150.030.2012254
CAACGCA94900.029.64435
AACGCAG98850.028.55416
ACGCAGA111000.025.251087
CGCAGAG110900.025.1889578
TGGTATC17650.024.770162
GTGGTAT19950.023.90391
TACATGG65400.023.7605062
GTACATG67600.023.3503281
GCAGAGT120500.022.708399
ACATGGG66300.022.4345073
GAGTACT75550.022.2583412-13
TATCACG1100.002870486121.600462
CAGAGTA115100.021.44146210-11
GTACTTT80000.021.0508914-15
AGAGTAC109750.020.30076810-11
Produced by FastQC (version 0.11.5)