FastQCFastQC Report
Thu 26 May 2016
SRR937975_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937975_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612174
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96620.5993149622807463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83420.5174379440432608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53190.32992716667059513No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21660.13435274356241944No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18120.1123948159441847No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17860.1107820867970827No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA16220.10060948756151632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42500.049.691381
GTATCAA70800.038.5625421
TATCAAC88700.030.5123482
ATCAACG89200.030.391813
TCAACGC89450.030.3040454
CAACGCA90650.029.955355
AACGCAG93300.029.1684276
TGGTATC20150.027.854412
GTGGTAT23050.025.5880851
ACGCAGA107250.025.4187767
CGCAGAG108100.025.2189048
TAAGGTG8750.023.9139185
GAGTACT72600.023.22371912-13
GCAGAGT119250.022.7414289
GTACTTT77000.021.71160714-15
TAGCGAG1100.002879924621.5882457
GTACATG64300.021.4523091
TACATGG62200.021.4118732
AGGTAAG8000.021.4042262
GTAAGGT8250.021.3281844
Produced by FastQC (version 0.11.5)