##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937975_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1612174 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48810488197924 33.0 31.0 34.0 31.0 34.0 2 32.71086619682491 34.0 31.0 34.0 31.0 34.0 3 32.77110597243226 34.0 31.0 34.0 31.0 34.0 4 36.230933509658385 37.0 35.0 37.0 35.0 37.0 5 36.16047027181929 37.0 35.0 37.0 35.0 37.0 6 36.217332620424344 37.0 37.0 37.0 35.0 37.0 7 36.19001360895288 37.0 37.0 37.0 35.0 37.0 8 36.185842222985855 37.0 37.0 37.0 35.0 37.0 9 37.877477865292455 39.0 38.0 39.0 35.0 39.0 10-11 37.84731021589481 39.0 38.0 39.0 35.0 39.0 12-13 37.76428474842045 39.0 38.0 39.0 35.0 39.0 14-15 39.00238714927793 40.0 38.0 41.0 36.0 41.0 16-17 38.906912033068394 40.0 38.0 41.0 35.5 41.0 18-19 39.163664405951224 40.0 39.0 41.0 36.0 41.0 20-21 39.1388141106357 40.0 39.0 41.0 36.0 41.0 22-23 39.027162080519844 40.0 38.5 41.0 35.0 41.0 24-25 38.91146644220785 40.0 38.0 41.0 35.0 41.0 26-27 38.74169475503265 40.0 38.0 41.0 35.0 41.0 28-29 38.61140950046335 40.0 38.0 41.0 34.5 41.0 30-31 38.35560429581422 40.0 38.0 41.0 34.0 41.0 32-33 38.11163652310482 40.0 37.5 41.0 34.0 41.0 34-35 37.99621784000982 40.0 37.0 41.0 33.0 41.0 36-37 37.93351772203249 40.0 37.0 41.0 33.0 41.0 38-39 37.731972479397385 40.0 37.0 41.0 33.0 41.0 40-41 37.50394529374621 40.0 37.0 41.0 33.0 41.0 42-43 37.231344445450674 40.0 36.0 41.0 32.0 41.0 44-45 36.935874787709025 40.0 36.0 41.0 31.0 41.0 46-47 36.53632176179494 39.0 35.5 41.0 30.5 41.0 48-49 36.09555265126468 39.0 35.0 41.0 29.5 41.0 50-51 35.691846847796825 39.0 35.0 40.0 28.0 41.0 52-53 35.14912689325098 38.0 34.5 40.0 27.0 41.0 54-55 34.756667704602606 38.0 34.0 40.0 26.0 41.0 56-57 34.35807208154951 38.0 34.0 40.0 25.0 41.0 58-59 33.834452732769535 37.0 33.0 40.0 23.0 41.0 60-61 33.339569426128946 36.5 32.5 39.5 22.0 41.0 62-63 32.77148775504381 36.0 32.0 39.0 19.5 40.0 64-65 32.19227236017949 35.5 31.0 39.0 17.5 40.0 66-67 31.612554228017572 35.0 31.0 38.0 12.0 40.0 68-69 30.971980691910424 35.0 30.0 38.0 7.5 40.0 70-71 30.336124388558552 34.5 29.0 37.0 4.5 39.0 72-73 29.652858190244974 34.0 28.5 36.5 2.0 39.0 74-75 28.99871043696276 34.0 27.5 36.0 2.0 38.5 76-77 27.695583417174575 31.5 26.0 34.5 2.0 36.5 78-79 28.343500453425 33.5 27.0 35.0 2.0 37.0 80-81 28.058662712585615 33.5 26.5 35.0 2.0 37.0 82-83 27.493176915146876 33.0 26.0 35.0 2.0 36.0 84-85 26.817928771956375 33.0 25.0 35.0 2.0 36.0 86-87 26.1012356606669 32.0 23.5 35.0 2.0 35.0 88-89 25.29035482522358 32.0 20.0 34.0 2.0 35.0 90-91 24.61728541708277 31.0 17.0 34.0 2.0 35.0 92-93 23.676602215393622 31.0 4.5 34.0 2.0 35.0 94-95 22.769304678031034 30.5 2.0 34.0 2.0 35.0 96-97 21.72630156546378 30.0 2.0 34.0 2.0 35.0 98-99 20.317132952150327 29.0 2.0 34.0 2.0 35.0 100-101 16.494564482493825 13.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19718678392544575 1101 2 0.09070373203417859 1101 3 0.18643899538624709 1101 4 0.0297014210450115 1101 5 -0.05774162734795141 1101 6 0.01778709198935502 1101 7 0.030122490099969923 1101 8 0.08516558884493719 1101 9 0.11770892940796784 1101 10-11 0.08526182789749015 1101 12-13 0.2923094383096796 1101 14-15 0.3435892716123661 1101 16-17 0.411470528940292 1101 18-19 0.24895613916430648 1101 20-21 0.1903302369851616 1101 22-23 0.30581434100114535 1101 24-25 0.38685834645290385 1101 26-27 0.4454552509185561 1101 28-29 0.3942749535636949 1101 30-31 0.45492593911792056 1101 32-33 0.5023913161475448 1101 34-35 0.4617971999783137 1101 36-37 0.5403836511745013 1101 38-39 0.7442881684764018 1101 40-41 0.8416021837531389 1101 42-43 0.8020510423401888 1101 44-45 0.9140244968757472 1101 46-47 0.8124840541076708 1101 48-49 1.0574667005453904 1101 50-51 1.057481247364727 1101 52-53 1.1651989856455458 1101 54-55 1.4414009678425401 1101 56-57 1.338331610121763 1101 58-59 1.3487899320825747 1101 60-61 1.37970335348691 1101 62-63 1.5392032843474723 1101 64-65 1.7395885352760558 1101 66-67 1.8702028832683197 1101 68-69 2.0203395684488363 1101 70-71 2.2302164602293892 1101 72-73 2.1193236380825944 1101 74-75 2.286537936450518 1101 76-77 2.346817148656296 1101 78-79 2.6156287652791654 1101 80-81 2.5912907055361565 1101 82-83 2.7339577685711625 1101 84-85 2.793640967349617 1101 86-87 3.2733389771106154 1101 88-89 3.56699059389112 1101 90-91 3.737090924577899 1101 92-93 3.7873894167606466 1101 94-95 4.368993173107086 1101 96-97 4.6406087375265095 1101 98-99 4.832922706001483 1101 100-101 4.495345108651339 1102 1 0.22662377696153868 1102 2 0.16650640368210645 1102 3 0.19018172489011675 1102 4 0.11220435639117454 1102 5 -0.0034138385863755616 1102 6 0.051344794186285014 1102 7 0.1264618824193562 1102 8 0.21590530651919693 1102 9 0.2626103545061298 1102 10-11 0.1835438477080089 1102 12-13 0.2894837271310351 1102 14-15 0.4307067956158974 1102 16-17 0.43376558591653946 1102 18-19 0.31123142007785276 1102 20-21 0.2674213807234125 1102 22-23 0.331747191140046 1102 24-25 0.34227136492848587 1102 26-27 0.3378699196616708 1102 28-29 0.11665829280513407 1102 30-31 0.3475682309693795 1102 32-33 0.4341007291461878 1102 34-35 0.3714394401447052 1102 36-37 0.45965262598821255 1102 38-39 0.46780779600981504 1102 40-41 0.6233343329590326 1102 42-43 0.733965629379135 1102 44-45 0.5107045407506163 1102 46-47 0.5288891543270964 1102 48-49 0.6388362189919974 1102 50-51 0.8749095030147132 1102 52-53 0.8864235875269841 1102 54-55 1.098575507258147 1102 56-57 0.9796946966493536 1102 58-59 1.1672351827211997 1102 60-61 1.0076546880839956 1102 62-63 1.5171376777292807 1102 64-65 1.3492887775693703 1102 66-67 1.3354752191773827 1102 68-69 1.8994468995313518 1102 70-71 1.8385911633665764 1102 72-73 2.0220659222596034 1102 74-75 1.8896313658171344 1102 76-77 1.6597135639577907 1102 78-79 1.8232163414428477 1102 80-81 2.1559899637249735 1102 82-83 1.9854554607547605 1102 84-85 2.32600573651316 1102 86-87 2.428633276561346 1102 88-89 2.7213146237389836 1102 90-91 2.6873602350834886 1102 92-93 3.0796389543359517 1102 94-95 3.2993485940610476 1102 96-97 3.622627817146043 1102 98-99 3.886525076623066 1102 100-101 3.565122032784455 1103 1 0.2532544630754714 1103 2 0.2887604523710863 1103 3 0.24331733887343177 1103 4 0.14286231250405734 1103 5 0.11581098959830172 1103 6 0.10599451739288668 1103 7 0.13205887584535958 1103 8 0.1455648624172312 1103 9 0.2773962172753244 1103 10-11 0.29031109424878565 1103 12-13 0.30061182868757186 1103 14-15 0.36203452711252737 1103 16-17 0.3933500927012261 1103 18-19 0.30290532243633805 1103 20-21 0.3021757488182928 1103 22-23 0.3762560901641976 1103 24-25 0.32861832841800975 1103 26-27 0.4376014683969984 1103 28-29 0.47069956656078915 1103 30-31 0.5376803762597362 1103 32-33 0.5958932200262055 1103 34-35 0.47686317778995857 1103 36-37 0.4331925402020431 1103 38-39 0.46368357692593065 1103 40-41 0.5937708743968813 1103 42-43 0.6525028455525543 1103 44-45 0.7119771039591711 1103 46-47 0.6723317136226115 1103 48-49 0.6773245046866663 1103 50-51 0.6372627453979405 1103 52-53 0.9321244342156589 1103 54-55 1.0470371732893398 1103 56-57 0.9329684943930161 1103 58-59 1.156485858984908 1103 60-61 1.2627440140147996 1103 62-63 1.317626982516657 1103 64-65 1.3479908623003638 1103 66-67 1.5157088288949367 1103 68-69 1.6866203602328973 1103 70-71 1.7231874318038116 1103 72-73 1.746959240688998 1103 74-75 1.8762361086565527 1103 76-77 1.9684600749180454 1103 78-79 1.9761326049900418 1103 80-81 2.1102618109632765 1103 82-83 2.276925494468994 1103 84-85 2.3365661723681903 1103 86-87 2.6637495879066897 1103 88-89 2.608889007736522 1103 90-91 2.7100310395805245 1103 92-93 3.0945826411969186 1103 94-95 3.34598786780969 1103 96-97 3.601983173370048 1103 98-99 4.152137010729753 1103 100-101 3.5446008926231336 1104 1 0.19716029198782792 1104 2 0.1455974797406654 1104 3 0.172907011768082 1104 4 0.1416028749617979 1104 5 -0.015227954564593915 1104 6 0.0970249680031614 1104 7 0.1549147246262237 1104 8 0.2197544437849004 1104 9 0.22926451636137557 1104 10-11 0.2532982258574137 1104 12-13 0.23327264591423358 1104 14-15 0.3213490446843892 1104 16-17 0.34989737881341654 1104 18-19 0.3095593900194089 1104 20-21 0.3418562707103803 1104 22-23 0.3812481710092541 1104 24-25 0.3779374317549795 1104 26-27 0.3310716496241781 1104 28-29 0.2771612109201058 1104 30-31 0.32981432956591306 1104 32-33 0.3503690491833922 1104 34-35 0.2793796046220507 1104 36-37 0.32758740753502025 1104 38-39 0.42710927528992926 1104 40-41 0.4730878025557388 1104 42-43 0.4129053288338085 1104 44-45 0.44717106204885937 1104 46-47 0.6480890710919454 1104 48-49 0.5326987012361428 1104 50-51 0.5623292719492454 1104 52-53 0.6084758224689466 1104 54-55 0.6946307765707473 1104 56-57 0.7474561693253605 1104 58-59 0.8099392602666597 1104 60-61 0.9438979789595905 1104 62-63 1.2009044297803584 1104 64-65 1.2436143286114714 1104 66-67 1.2451023120236542 1104 68-69 1.4205289858028287 1104 70-71 1.4877811348985865 1104 72-73 1.666391961770902 1104 74-75 1.643708862432046 1104 76-77 1.6089327947946543 1104 78-79 1.707970338586282 1104 80-81 1.9314101449275078 1104 82-83 1.9290902675065773 1104 84-85 2.1110013793679308 1104 86-87 2.1464592760181667 1104 88-89 2.31337787854601 1104 90-91 2.5089671326593113 1104 92-93 2.9026514220237125 1104 94-95 3.230531344064463 1104 96-97 3.4514989843724955 1104 98-99 3.736876676750459 1104 100-101 3.3517313336287238 1105 1 0.1997127593729644 1105 2 0.16214890272998161 1105 3 0.20035976636206954 1105 4 0.19753252229813967 1105 5 0.04499677060624663 1105 6 0.054943469115080745 1105 7 0.13479795036877817 1105 8 0.13806288268324352 1105 9 0.19116477655999375 1105 10-11 0.26204233987662917 1105 12-13 0.3227647634267683 1105 14-15 0.44770540874636566 1105 16-17 0.4208262608597195 1105 18-19 0.2767081894143786 1105 20-21 0.35287194565118085 1105 22-23 0.3736038238016661 1105 24-25 0.3851500653431046 1105 26-27 0.4154014356130631 1105 28-29 0.48688467111152534 1105 30-31 0.512485461925074 1105 32-33 0.5360389930921272 1105 34-35 0.5372733463501689 1105 36-37 0.604861219309683 1105 38-39 0.4422608927529481 1105 40-41 0.4799796895809223 1105 42-43 0.5442576863773283 1105 44-45 0.5308359460398862 1105 46-47 0.4028584036689935 1105 48-49 0.5713737590830661 1105 50-51 0.6394137869804339 1105 52-53 0.7328597639827734 1105 54-55 0.9038743548869377 1105 56-57 0.9768426317946322 1105 58-59 0.9988189206579463 1105 60-61 1.0709651624221195 1105 62-63 1.1664436441426034 1105 64-65 1.476922452390383 1105 66-67 1.5124242020199574 1105 68-69 1.7823452891818015 1105 70-71 1.867331459471604 1105 72-73 1.94710215093259 1105 74-75 1.7532968212684423 1105 76-77 1.7633651746666281 1105 78-79 1.925880376490646 1105 80-81 2.2041917041384487 1105 82-83 2.1083304245260877 1105 84-85 2.5029822651236344 1105 86-87 2.624585485851693 1105 88-89 2.7103882954940346 1105 90-91 2.921024277317141 1105 92-93 3.2909789481561162 1105 94-95 3.495789201792544 1105 96-97 3.7190691434627894 1105 98-99 3.5376806814682453 1105 100-101 3.29133554362431 1106 1 0.057101631907556794 1106 2 0.07156056848548076 1106 3 0.029059459368504292 1106 4 0.01956246468427736 1106 5 -0.01690028141444344 1106 6 -0.0032717628122469478 1106 7 0.0537432382278169 1106 8 0.04200753973358218 1106 9 0.1254807696631488 1106 10-11 0.12451447915918834 1106 12-13 0.04521734763973484 1106 14-15 0.08763665059841941 1106 16-17 0.0761040269558535 1106 18-19 -0.06214226687058044 1106 20-21 -0.01904129712605851 1106 22-23 -0.021632519190049493 1106 24-25 -0.02333010623686249 1106 26-27 -0.1295394786390034 1106 28-29 -0.05053595212746842 1106 30-31 0.07442772190236724 1106 32-33 0.23603163365733337 1106 34-35 0.17525538932926565 1106 36-37 0.30327263274170235 1106 38-39 0.0697441763224802 1106 40-41 0.10001312244185101 1106 42-43 0.026791644912158574 1106 44-45 0.09360771945340218 1106 46-47 0.36893140926216006 1106 48-49 0.3556781873601196 1106 50-51 0.5025577375250379 1106 52-53 0.6631806353398559 1106 54-55 0.6032908568424418 1106 56-57 0.5557946482797647 1106 58-59 0.5822318059769316 1106 60-61 0.675086445907553 1106 62-63 0.6503958708972988 1106 64-65 0.79630720364149 1106 66-67 0.981565040574992 1106 68-69 0.9957142281615781 1106 70-71 0.790219301685319 1106 72-73 1.2310964316004096 1106 74-75 1.4212559448648925 1106 76-77 1.337004730577302 1106 78-79 1.656437801109007 1106 80-81 1.6404295610710378 1106 82-83 1.7719464302454178 1106 84-85 1.882364282870384 1106 86-87 2.0065927191247503 1106 88-89 2.080697181485874 1106 90-91 2.2297262832887945 1106 92-93 2.14658473477456 1106 94-95 2.583623004727908 1106 96-97 3.026107917170684 1106 98-99 3.045734005830081 1106 100-101 3.0942204420641097 1107 1 0.24713637998399918 1107 2 0.2536239423582316 1107 3 0.2973849330178808 1107 4 0.1434600405922808 1107 5 0.14286227339650992 1107 6 0.13207199494402033 1107 7 0.1744348904161157 1107 8 0.1182878408835748 1107 9 0.2323890181946311 1107 10-11 0.23452730285406886 1107 12-13 0.2691645636569362 1107 14-15 0.41657062925317945 1107 16-17 0.2996582587751604 1107 18-19 0.19819321753414698 1107 20-21 0.38574276839889166 1107 22-23 0.37840264255397216 1107 24-25 0.42297170562712694 1107 26-27 0.5246592472648643 1107 28-29 0.4139817114780584 1107 30-31 0.4452455427015778 1107 32-33 0.4400669608683856 1107 34-35 0.511676751122927 1107 36-37 0.49701591064357586 1107 38-39 0.5871244195590251 1107 40-41 0.5405794333543952 1107 42-43 0.6293129487923679 1107 44-45 0.5831626131408356 1107 46-47 0.7577375646357041 1107 48-49 0.6670602507339893 1107 50-51 0.7618032078650714 1107 52-53 0.8954756630555849 1107 54-55 0.842889185625431 1107 56-57 0.8981376614343972 1107 58-59 1.074481743926789 1107 60-61 1.0146496129465206 1107 62-63 1.2648411426769002 1107 64-65 1.3365363754789996 1107 66-67 1.485461789147834 1107 68-69 1.6194091069569758 1107 70-71 1.7762993877281765 1107 72-73 1.8663202257594236 1107 74-75 1.8401977832036032 1107 76-77 1.865293387812347 1107 78-79 1.8382509060978371 1107 80-81 1.8920470012960742 1107 82-83 2.329030487497011 1107 84-85 2.337388275589838 1107 86-87 2.465341788372701 1107 88-89 2.7719543172515735 1107 90-91 2.893627582204985 1107 92-93 2.8267866045520087 1107 94-95 2.7328866764413178 1107 96-97 3.0072219239548303 1107 98-99 3.454723002272541 1107 100-101 3.1839408027822387 1108 1 0.12407000303744553 1108 2 0.10627884776806695 1108 3 0.023708936673870085 1108 4 0.04336260496015143 1108 5 0.036872137676432715 1108 6 0.09908787781431982 1108 7 0.09807208495972475 1108 8 0.01033692084977389 1108 9 0.09825723003521603 1108 10-11 0.040496744280595465 1108 12-13 0.1356911127110365 1108 14-15 0.23654450270756655 1108 16-17 0.055450074182061826 1108 18-19 0.14932278105700902 1108 20-21 0.17734993897159512 1108 22-23 0.21663190083599915 1108 24-25 0.20153629769086479 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62-63 0.3447314262104868 2316 64-65 0.5169417831227108 2316 66-67 0.8035510927861651 2316 68-69 1.1034233647972975 2316 70-71 1.1696405390222786 2316 72-73 1.3113578082869424 2316 74-75 1.7633437504940517 2316 76-77 1.7806318293217451 2316 78-79 1.849282181253617 2316 80-81 1.8074437462417272 2316 82-83 1.9435169037901723 2316 84-85 2.229258590196501 2316 86-87 2.2803309061688317 2316 88-89 2.4808339968476893 2316 90-91 2.8674281380752475 2316 92-93 3.008986333573535 2316 94-95 3.4811369108145094 2316 96-97 3.815114089099314 2316 98-99 4.215683385750136 2316 100-101 4.525427176010361 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 13.0 5 27.0 6 70.0 7 188.0 8 405.0 9 812.0 10 1383.0 11 2306.0 12 3502.0 13 5515.0 14 7333.0 15 10235.0 16 13422.0 17 18659.0 18 19566.0 19 16279.0 20 15477.0 21 16290.0 22 17976.0 23 19709.0 24 21882.0 25 24274.0 26 27246.0 27 31966.0 28 37454.0 29 45052.0 30 53713.0 31 63953.0 32 77631.0 33 96103.0 34 125196.0 35 172911.0 36 229985.0 37 279885.0 38 149093.0 39 6662.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.5145337910176 18.783270292164495 14.950123249723665 22.752072667094247 2 15.90743142936727 19.95321197728715 39.859958522312404 24.279398071033174 3 19.47896390746675 24.053955812606148 29.79138210595397 26.675698173973135 4 13.095596239792325 16.786765458442012 36.066544882749376 34.05109341901628 5 13.694494158325707 37.20346066572856 34.039505044320215 15.062540131625521 6 32.57576415448953 35.7459554613832 16.882296824040086 14.795983560087187 7 28.717929950489214 33.04457211194325 19.949831717916304 18.28766621965123 8 26.841338072287662 34.77449648610258 19.11479558607343 19.26936985553633 9 26.37339393886764 16.819772555567823 20.00305177977067 36.80378172579387 10-11 25.372044208627603 26.58732866303513 26.461845929781774 21.57878119855549 12-13 26.288756672666846 23.841626276071935 27.267125012560676 22.602492038700536 14-15 22.850952456419858 25.275660467065116 25.862787100768 26.010599975747027 16-17 22.049481011354853 28.564596625426287 26.788702708268463 22.597219654950397 18-19 22.037602640906005 26.597439234226577 29.553974943151296 21.810983181716118 20-21 22.541921653618033 25.67362455913568 30.094084137320166 21.690369649926126 22-23 22.252688377136053 25.631113067065137 30.053998597543746 22.06219995825506 24-25 22.16105889708765 25.914172716491084 30.05307139206336 21.871696994357908 26-27 21.67415492665062 26.288103746776674 29.886004345940865 22.151736980631842 28-29 21.769176279979703 26.600447594366365 29.738694458538596 21.891681667115336 30-31 21.886936521740207 26.185635049318496 29.729514308008937 22.197914120932356 32-33 21.559335406724088 26.214509103856038 29.757333885796445 22.46882160362343 34-35 22.294971829989862 26.34457362850326 29.51039641488389 21.850058126622987 36-37 22.347281989253563 25.90464670900111 29.5729183627581 22.17515293898723 38-39 21.84672683387684 26.302414980059712 29.473444896384322 22.377413289679126 40-41 22.24874876861959 26.119625660354885 29.688457654161382 21.94316791686414 42-43 22.02435488583376 26.15321581634647 29.372350858735828 22.45007843908394 44-45 21.917836685082392 26.444147871263805 29.515913377335472 22.122102066318323 46-47 22.424378510011948 26.680401743236153 28.81509688160211 22.080122865149793 48-49 22.182841306211365 26.7833682964742 28.636145974317905 22.397644422996525 50-51 22.3408577486053 26.947587543280065 28.59607585781684 22.115478850297794 52-53 22.583263345023553 26.762899041914828 28.42478541398137 22.22905219908025 54-55 22.17896455345391 26.99513203909752 28.451364430886493 22.374538976562082 56-57 22.178692305759895 27.173719205780273 28.290224346200954 22.35736414225888 58-59 22.236185424153966 26.750803573311565 28.333169992817155 22.679841009717315 60-61 21.963603184271673 27.006048974862512 28.614994411273226 22.41535342959259 62-63 22.053326750090253 27.0267353275763 28.70205077119467 22.217887151138775 64-65 21.890875302541783 27.0646344625332 28.491589617497866 22.552900617427152 66-67 21.754458113366244 27.186164264008887 28.294668128048293 22.764709494576575 68-69 22.212849022880153 26.76220713619572 28.513148341527817 22.51179549939631 70-71 22.102887157341577 26.91409239945564 28.119917577134974 22.863102866067806 72-73 22.067165847844542 27.062626944308416 28.033645262125944 22.836561945721105 74-75 21.990802481617987 27.363981803452976 27.921272765842893 22.72394294908614 76-77 22.000385814434424 27.0092124051126 28.25197528306498 22.738426497387998 78-79 22.054660353038816 27.170733431999277 28.18594643009998 22.58865978486193 80-81 22.22311818369312 27.166664857513695 27.95873270385148 22.6514842549417 82-83 22.128065403546206 27.275958428057027 28.060813388373873 22.535162780022887 84-85 22.005438848313943 27.370749814218172 27.961902008924973 22.66190932854291 86-87 22.237629762695274 27.23711646518835 27.806768145532097 22.718485626584282 88-89 22.118301126305226 27.46055946814674 27.47746211016925 22.943677295378787 90-91 22.01727277915208 27.35188557760779 27.6807978503001 22.95004379294003 92-93 22.107322162496107 27.039264992488405 27.76921101568441 23.08420182933108 94-95 21.90697837422585 26.83808535495714 27.968546809633082 23.286389461183923 96-97 22.115630342171926 26.60246549375625 27.88288098292242 23.399023181149406 98-99 21.822055187591413 26.88072131171945 27.57332645235564 23.72389704833349 100-101 22.21373127218278 26.142463530611458 27.908494988754317 23.735310208451445 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1667.0 1 1102.5 2 936.0 3 1257.5 4 885.5 5 740.0 6 1436.5 7 3072.5 8 4698.0 9 5055.5 10 3904.0 11 2596.5 12 2271.5 13 2259.0 14 2344.5 15 2445.5 16 2472.5 17 2592.5 18 2752.5 19 2955.5 20 3243.0 21 3678.0 22 4341.5 23 5298.5 24 6540.5 25 8044.0 26 9869.0 27 11950.0 28 13804.5 29 15666.0 30 17972.5 31 21188.0 32 25513.5 33 27731.0 34 28423.5 35 30381.5 36 33221.5 37 36357.0 38 39451.0 39 42091.5 40 44212.5 41 46738.5 42 48206.0 43 49916.0 44 52761.5 45 55603.5 46 56553.5 47 57552.0 48 59307.0 49 58998.0 50 57947.0 51 56704.0 52 55988.0 53 56056.0 54 56468.0 55 53719.0 56 49088.5 57 45237.0 58 42067.5 59 37712.0 60 30729.5 61 25163.0 62 21040.5 63 15863.0 64 11400.0 65 8719.0 66 6653.0 67 5206.0 68 4026.5 69 3115.5 70 2373.5 71 1588.5 72 1120.5 73 871.5 74 677.5 75 541.5 76 347.5 77 207.0 78 139.5 79 75.0 80 35.5 81 24.5 82 21.0 83 9.5 84 6.0 85 3.0 86 1.5 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01383225383860551 3 0.002481121764772289 4 0.0017988132794599092 5 0.11462782553247974 6 0.0 7 0.0 8 2.4811217647722887E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 3.101402205965361E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.5507011029826807E-4 24-25 3.7216826471584335E-4 26-27 0.005892664191334186 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010916935764998071 36-37 7.133225073720331E-4 38-39 0.006885112897243101 40-41 0.01004854314732777 42-43 0.005892664191334186 44-45 0.0034425564486215503 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.101402205965361E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.202804411930722E-5 68-69 7.753505514913402E-4 70-71 0.0 72-73 3.101402205965361E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.304206617896084E-5 82-83 0.0017988132794599092 84-85 0.0042179070001128905 86-87 0.0030083601397864 88-89 0.0 90-91 0.0026051778530109034 92-93 0.0 94-95 3.101402205965361E-5 96-97 1.2405608823861443E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1612174.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.127801622924004 #Duplication Level Percentage of deduplicated Percentage of total 1 79.82067020619432 46.39800083154505 2 11.860930675944168 13.78899650789073 3 3.4458694175473674 6.0090244176508225 4 1.4763881952849018 3.4327680053579024 5 0.8371822549269328 2.433178201831247 6 0.5121599677178347 1.786243980162327 7 0.35838943736221734 1.4582673083119624 8 0.2536364504042228 1.1794663418751412 9 0.21043414103693922 1.1008866604397052 >10 1.1163605499129652 11.882476375639932 >50 0.0692189181367518 2.7809790086866837 >100 0.03352799304136858 3.6088159514851905 >500 0.0034166808098267092 1.4126961040836863 >1k 0.0014947978542991852 1.2806864119043522 >5k 3.20313825921254E-4 1.4475138931352172 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9662 0.5993149622807463 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8342 0.5174379440432608 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5319 0.32992716667059513 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2166 0.13435274356241944 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1812 0.1123948159441847 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1786 0.1107820867970827 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1622 0.10060948756151632 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 6.202804411930722E-5 0.0 0.0 0.0 0.0 6 6.202804411930722E-5 0.0 0.0 0.0 0.0 7 6.202804411930722E-5 0.0 0.0 0.0 0.0 8 6.202804411930722E-5 0.0 0.0 0.0 0.0 9 6.202804411930722E-5 0.0 0.0 6.202804411930722E-5 0.0 10-11 6.202804411930722E-5 0.0 0.0 6.202804411930722E-5 6.202804411930722E-5 12-13 6.202804411930722E-5 0.0 0.0 6.202804411930722E-5 1.8608413235792168E-4 14-15 6.202804411930722E-5 0.0 0.0 6.202804411930722E-5 2.7912619853688245E-4 16-17 6.202804411930722E-5 0.0 0.0 9.304206617896082E-5 3.101402205965361E-4 18-19 6.202804411930722E-5 0.0 0.0 1.2405608823861443E-4 3.101402205965361E-4 20-21 6.202804411930722E-5 0.0 0.0 1.5507011029826807E-4 3.101402205965361E-4 22-23 6.202804411930722E-5 0.0 0.0 3.7216826471584335E-4 3.411542426561897E-4 24-25 6.202804411930722E-5 0.0 0.0 7.443365294316866E-4 3.7216826471584335E-4 26-27 6.202804411930722E-5 0.0 0.0 0.0016127291471019877 3.7216826471584335E-4 28-29 9.304206617896082E-5 0.0 0.0 0.004869201463365617 3.7216826471584335E-4 30-31 1.2405608823861443E-4 0.0 0.0 0.012746763066517633 3.7216826471584335E-4 32-33 1.2405608823861443E-4 0.0 0.0 0.028222760074284786 3.7216826471584335E-4 34-35 1.8608413235792165E-4 0.0 0.0 0.05188645890580049 3.7216826471584335E-4 36-37 2.4811217647722887E-4 0.0 0.0 0.09009573408329374 3.7216826471584335E-4 38-39 2.4811217647722887E-4 0.0 0.0 0.15113132949669206 3.7216826471584335E-4 40-41 2.4811217647722887E-4 0.0 0.0 0.2151442710278171 3.7216826471584335E-4 42-43 2.7912619853688245E-4 0.0 0.0 0.278723016250107 3.7216826471584335E-4 44-45 4.3419630883515057E-4 0.0 0.0 0.35455230018596007 3.7216826471584335E-4 46-47 4.6521033089480415E-4 0.0 0.0 0.4463538054825348 3.7216826471584335E-4 48-49 4.962243529544577E-4 0.0 0.0 0.5371938760952601 4.3419630883515057E-4 50-51 4.962243529544577E-4 0.0 0.0 0.6397882610685943 4.3419630883515057E-4 52-53 4.962243529544577E-4 0.0 0.0 0.739653412100679 4.3419630883515057E-4 54-55 4.962243529544577E-4 0.0 0.0 0.8442016804637713 4.3419630883515057E-4 56-57 4.962243529544577E-4 0.0 0.0 0.9654044786728976 4.3419630883515057E-4 58-59 4.962243529544577E-4 0.0 0.0 1.0947949787057725 4.3419630883515057E-4 60-61 4.962243529544577E-4 0.0 0.0 1.2271007968122547 4.3419630883515057E-4 62-63 4.962243529544577E-4 0.0 0.0 1.3594066149187372 4.3419630883515057E-4 64-65 4.962243529544577E-4 0.0 0.0 1.4953410736061987 4.3419630883515057E-4 66-67 5.58252397073765E-4 0.0 0.0 1.6559937078752045 4.3419630883515057E-4 68-69 5.58252397073765E-4 0.0 0.0 1.830912792291651 4.3419630883515057E-4 70-71 5.58252397073765E-4 0.0 0.0 2.0102978958846873 4.3419630883515057E-4 72-73 5.58252397073765E-4 0.0 0.0 2.1927533876616296 5.272383750141113E-4 74-75 5.58252397073765E-4 0.0 0.0 2.3746506270414978 5.58252397073765E-4 76-77 5.58252397073765E-4 0.0 0.0 2.5660691711936803 5.58252397073765E-4 78-79 5.58252397073765E-4 0.0 0.0 2.787323204567249 5.58252397073765E-4 80-81 6.202804411930722E-4 0.0 0.0 3.01822259880137 5.58252397073765E-4 82-83 6.202804411930722E-4 0.0 0.0 3.2580540313886717 5.58252397073765E-4 84-85 6.823084853123794E-4 0.0 0.0 3.4970170713583024 5.58252397073765E-4 86-87 7.443365294316867E-4 0.0 0.0 3.7454704020781877 6.202804411930723E-4 88-89 7.443365294316867E-4 0.0 0.0 4.006019201401338 6.823084853123794E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4250 0.0 49.69138 1 GTATCAA 7080 0.0 38.562542 1 TATCAAC 8870 0.0 30.512348 2 ATCAACG 8920 0.0 30.39181 3 TCAACGC 8945 0.0 30.304045 4 CAACGCA 9065 0.0 29.95535 5 AACGCAG 9330 0.0 29.168427 6 TGGTATC 2015 0.0 27.85441 2 GTGGTAT 2305 0.0 25.588085 1 ACGCAGA 10725 0.0 25.418776 7 CGCAGAG 10810 0.0 25.218904 8 TAAGGTG 875 0.0 23.913918 5 GAGTACT 7260 0.0 23.223719 12-13 GCAGAGT 11925 0.0 22.741428 9 GTACTTT 7700 0.0 21.711607 14-15 TAGCGAG 110 0.0028799246 21.588245 7 GTACATG 6430 0.0 21.452309 1 TACATGG 6220 0.0 21.411873 2 AGGTAAG 800 0.0 21.404226 2 GTAAGGT 825 0.0 21.328184 4 >>END_MODULE