FastQCFastQC Report
Thu 26 May 2016
SRR937974_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937974_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1616566
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101920.6304722479626567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83620.5172693227495816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54010.33410327818350755No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21060.13027615327799794No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18070.1117801562076649No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16550.10237750886756247No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA16250.10052172320833173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43850.052.6988221
GTATCAA77750.042.3715021
TATCAAC96650.033.776762
ATCAACG96750.033.4471633
TCAACGC97600.033.1071854
CAACGCA98550.032.739825
AACGCAG101600.031.8465756
ACGCAGA113800.028.2984337
CGCAGAG115300.027.8066988
GTGTAAG8350.025.1588821
GCAGAGT124600.025.006959
ACATGGG61150.024.4003663
GAGTACT78700.024.38570812-13
TACATGG63500.024.2456822
GTACATG64700.024.1306291
GTACTTT83050.022.82455314-15
CAGAGTA118450.022.37660810-11
GTATAGA3650.022.2372571
TGGTATC20200.021.642112
TGTAAGG8450.021.3692862
Produced by FastQC (version 0.11.5)