FastQCFastQC Report
Thu 26 May 2016
SRR937974_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937974_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1616566
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101130.6255853457266823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86150.5329197818090942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54840.3392376185073792No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21500.13299797224486967No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18160.11233689190543411No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17570.10868718010894698No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA17070.10559420401022909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44650.049.473951
GTATCAA72400.038.6475371
TATCAAC91150.030.4890792
ATCAACG90850.030.427243
TCAACGC92200.030.1344014
CAACGCA93550.029.6995375
AACGCAG96400.028.8214916
ACGCAGA109250.025.4315037
CGCAGAG111550.024.821998
TGGTATC21500.023.8632952
GTGGTAT23100.023.649991
GCAGAGT121950.022.3546419
GAGTACT76700.022.2917812-13
TACATGG62250.022.2837682
GTACATG64650.021.9708941
GTACTTT79700.021.24412214-15
CAGAGTA114700.021.179710-11
ACATGGG62500.021.0505893
GACCGCG1604.995856E-520.778577
AGTACTT79200.020.53870212-13
Produced by FastQC (version 0.11.5)