##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937974_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1616566 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50345794727836 33.0 31.0 34.0 31.0 34.0 2 32.73360320580787 34.0 31.0 34.0 31.0 34.0 3 32.69061393101179 34.0 31.0 34.0 31.0 34.0 4 36.21517216123561 37.0 35.0 37.0 35.0 37.0 5 36.1826779729377 37.0 35.0 37.0 35.0 37.0 6 36.242864194842646 37.0 37.0 37.0 35.0 37.0 7 36.20532350674207 37.0 37.0 37.0 35.0 37.0 8 36.20306625278523 37.0 37.0 37.0 35.0 37.0 9 37.909877481030776 39.0 38.0 39.0 35.0 39.0 10-11 37.87577030569739 39.0 38.0 39.0 35.0 39.0 12-13 37.813237442826335 39.0 38.0 39.0 35.0 39.0 14-15 39.086278877571345 40.0 38.5 41.0 36.0 41.0 16-17 39.02340176646051 40.0 38.0 41.0 36.0 41.0 18-19 39.023347639378784 40.0 38.5 41.0 36.0 41.0 20-21 39.03380406367572 40.0 38.5 41.0 35.5 41.0 22-23 39.052109224120755 40.0 39.0 41.0 35.0 41.0 24-25 38.99394024122739 40.0 38.5 41.0 35.0 41.0 26-27 38.816955818692215 40.0 38.0 41.0 35.0 41.0 28-29 38.73628048591892 40.0 38.0 41.0 35.0 41.0 30-31 38.50242025379725 40.0 38.0 41.0 34.0 41.0 32-33 38.28644360947836 40.0 38.0 41.0 34.0 41.0 34-35 38.072182948299044 40.0 37.5 41.0 34.0 41.0 36-37 37.98103789143159 40.0 37.0 41.0 33.0 41.0 38-39 37.92588610672252 40.0 37.0 41.0 33.0 41.0 40-41 37.79520662936125 40.0 37.0 41.0 33.0 41.0 42-43 37.59436731936711 40.0 37.0 41.0 33.0 41.0 44-45 37.333260132899 40.0 37.0 41.0 32.5 41.0 46-47 36.95818729331187 40.0 36.0 41.0 31.0 41.0 48-49 36.563033306403824 39.5 36.0 41.0 30.5 41.0 50-51 36.25276140906094 39.0 35.0 41.0 30.0 41.0 52-53 35.78097151616451 39.0 35.0 40.0 28.5 41.0 54-55 35.45911982560563 38.5 35.0 40.0 28.0 41.0 56-57 35.17963046358763 38.0 35.0 40.0 27.0 41.0 58-59 34.79063304560408 38.0 34.0 40.0 26.5 41.0 60-61 34.36430680838271 37.0 34.0 40.0 26.0 41.0 62-63 33.92782447484359 37.0 33.0 40.0 25.0 41.0 64-65 33.485655704746975 36.0 33.0 39.0 23.5 41.0 66-67 32.963688151303444 35.5 32.0 39.0 22.0 40.0 68-69 32.418518946952986 35.0 31.5 38.5 20.5 40.0 70-71 31.824428139649108 35.0 31.0 37.5 19.0 40.0 72-73 31.346129697148154 35.0 31.0 37.0 18.0 39.0 74-75 30.790565618725125 34.0 30.0 36.5 14.0 39.0 76-77 29.58378408305012 33.5 28.5 35.0 13.0 37.5 78-79 30.277894932839118 34.0 30.0 35.5 11.0 37.5 80-81 30.078967082073977 34.0 30.0 35.0 7.0 37.0 82-83 29.611779537612446 34.0 29.5 35.0 4.5 37.0 84-85 29.09910421226229 34.0 29.0 35.0 2.0 36.0 86-87 28.555724913180164 34.0 29.0 35.0 2.0 36.0 88-89 27.916853070026214 33.0 27.0 35.0 2.0 35.0 90-91 27.370516885793712 33.0 26.0 35.0 2.0 35.0 92-93 26.726702776131628 33.0 25.0 35.0 2.0 35.0 94-95 25.970218042443058 32.0 23.5 34.0 2.0 35.0 96-97 25.14319798882322 32.0 19.5 34.0 2.0 35.0 98-99 24.105557088297047 31.5 6.0 34.0 2.0 35.0 100-101 20.606473846412705 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07996664806953646 1101 2 0.04717247163911509 1101 3 0.16851464976070218 1101 4 0.03704528768657411 1101 5 -0.03724297577986846 1101 6 -0.013071403112839164 1101 7 0.07677139818672174 1101 8 0.10369297126310073 1101 9 0.09820633183790761 1101 10-11 0.11497886774643007 1101 12-13 0.08902312940641366 1101 14-15 0.1492931632428025 1101 16-17 0.19169256138887647 1101 18-19 0.22251694421013468 1101 20-21 0.14814894584206684 1101 22-23 0.025705798308159444 1101 24-25 0.11913386199310594 1101 26-27 0.18296708991221777 1101 28-29 0.09897256919703068 1101 30-31 0.03517359122079711 1101 32-33 -0.00681078912482036 1101 34-35 -0.04548706713473649 1101 36-37 0.03441437568081085 1101 38-39 0.2033429649295968 1101 40-41 0.23795026773387207 1101 42-43 0.23669366852043794 1101 44-45 0.3153806316665353 1101 46-47 0.20568932964090436 1101 48-49 0.3347538945615085 1101 50-51 0.4457017282955462 1101 52-53 0.49826825237165195 1101 54-55 0.47540962282666044 1101 56-57 0.4252447174631868 1101 58-59 0.478253054310926 1101 60-61 0.5322930657699843 1101 62-63 0.6177292520726567 1101 64-65 0.45183443034603243 1101 66-67 0.5336016260281866 1101 68-69 0.5731198404529323 1101 70-71 0.6258145512060338 1101 72-73 0.7535832291255069 1101 74-75 0.7553725030760283 1101 76-77 0.9766810360231339 1101 78-79 1.0881977458230416 1101 80-81 1.2117501844110556 1101 82-83 1.3689876573242437 1101 84-85 1.4691934269601035 1101 86-87 1.561281504623441 1101 88-89 1.8095607369341131 1101 90-91 1.877097903338253 1101 92-93 2.1399797857304073 1101 94-95 2.256709694022618 1101 96-97 2.3956833861104236 1101 98-99 2.6188648207628162 1101 100-101 2.5559034960511404 1102 1 0.029230723629041222 1102 2 0.07494854854571287 1102 3 0.20836478600048736 1102 4 0.018231951495778276 1102 5 -0.0561787746960718 1102 6 -0.01556466766293596 1102 7 0.06116122765737941 1102 8 -0.029672763666333424 1102 9 0.0017152716168595816 1102 10-11 0.10989092420174984 1102 12-13 0.14885382468853692 1102 14-15 0.11291599335638125 1102 16-17 0.13881162577365558 1102 18-19 0.13345592715720045 1102 20-21 0.12247291332820964 1102 22-23 0.15441316597186727 1102 24-25 0.04801937465268935 1102 26-27 0.06107553830948831 1102 28-29 -0.11343520106289162 1102 30-31 -0.02953456479671246 1102 32-33 -0.03823874277268402 1102 34-35 -0.07409550787808428 1102 36-37 0.09121890220929885 1102 38-39 0.1788772085691832 1102 40-41 0.05792623442400924 1102 42-43 -0.0247498991249131 1102 44-45 0.1722676291866705 1102 46-47 0.17797352735697558 1102 48-49 0.04974103597533741 1102 50-51 0.1394081009496233 1102 52-53 0.04045953149481818 1102 54-55 0.018419871425990664 1102 56-57 0.14591585146802544 1102 58-59 0.11037311370534297 1102 60-61 0.18292126128172725 1102 62-63 -0.0571848326715525 1102 64-65 -0.09149536942741321 1102 66-67 0.09435653219112083 1102 68-69 0.1918885925577598 1102 70-71 0.5578037285626749 1102 72-73 0.5949777351414944 1102 74-75 0.4964853448235722 1102 76-77 0.46206683187776676 1102 78-79 0.6432533515542964 1102 80-81 0.7652061207916674 1102 82-83 0.8002688470496189 1102 84-85 0.9925053548295573 1102 86-87 0.9247202579528953 1102 88-89 1.010037958867187 1102 90-91 1.1511666125811253 1102 92-93 1.3692711475159136 1102 94-95 1.357113821016469 1102 96-97 1.6582319885045678 1102 98-99 1.9132068515009593 1102 100-101 2.1601498910546617 1103 1 0.373006331045282 1103 2 0.31914531121311995 1103 3 0.44021391626436923 1103 4 0.2367330252029447 1103 5 0.1114411263052375 1103 6 0.10977149039741363 1103 7 0.245334761830577 1103 8 0.21999357655782603 1103 9 0.2407533543556042 1103 10-11 0.2823477816041091 1103 12-13 0.26086135729392623 1103 14-15 0.40198139067244654 1103 16-17 0.39795978847971014 1103 18-19 0.455663011625866 1103 20-21 0.40785306340293914 1103 22-23 0.4702090972101942 1103 24-25 0.4464663299655953 1103 26-27 0.5174682597518725 1103 28-29 0.5028396790749596 1103 30-31 0.500236936550138 1103 32-33 0.5732966140600766 1103 34-35 0.5396141892410071 1103 36-37 0.5728739990428124 1103 38-39 0.645643084889663 1103 40-41 0.6366171999610017 1103 42-43 0.5317024480411376 1103 44-45 0.6189334186778908 1103 46-47 0.6958037839141369 1103 48-49 0.5626208029797723 1103 50-51 0.5299499288944247 1103 52-53 0.7406823229557915 1103 54-55 0.9549348533606192 1103 56-57 1.0204564447051112 1103 58-59 1.087358916787224 1103 60-61 1.1759255094757393 1103 62-63 1.153893837752534 1103 64-65 1.155163036245142 1103 66-67 1.1954510028768084 1103 68-69 1.3584619899360035 1103 70-71 1.2008119184944803 1103 72-73 1.2251153184454004 1103 74-75 1.1007942749137989 1103 76-77 0.8816667621478729 1103 78-79 1.2203315356456663 1103 80-81 1.6559369855978332 1103 82-83 1.5700083481477556 1103 84-85 1.687506454074743 1103 86-87 1.6930601645503671 1103 88-89 1.888092805650171 1103 90-91 1.8591110462922025 1103 92-93 2.107692153980363 1103 94-95 2.1018146819808265 1103 96-97 2.2875018343215494 1103 98-99 2.6535384904422195 1103 100-101 2.9955171055673624 1104 1 0.01864791730859139 1104 2 -0.016221731803241823 1104 3 0.03439281921030357 1104 4 0.040161753884902396 1104 5 -0.024143047350882796 1104 6 -0.018488304296191416 1104 7 -0.009594149053619105 1104 8 -0.07531014172877804 1104 9 -0.09403003666250953 1104 10-11 -0.0649002085010224 1104 12-13 -0.047769585636693535 1104 14-15 0.006119422857985057 1104 16-17 0.02804160560309299 1104 18-19 -0.016198460235607115 1104 20-21 0.04714630014640164 1104 22-23 0.04550096101062451 1104 24-25 -0.04261968007745054 1104 26-27 -0.07541405876789042 1104 28-29 -0.1329765945338366 1104 30-31 -0.11375362522247201 1104 32-33 -0.09803322038537488 1104 34-35 -0.18657968906604339 1104 36-37 -0.18757238889222805 1104 38-39 -0.11887601459275743 1104 40-41 0.01601670370560271 1104 42-43 -0.217538879963719 1104 44-45 -0.2035917683251185 1104 46-47 -0.2596723385197208 1104 48-49 -0.2309750527886294 1104 50-51 -0.08667916328478498 1104 52-53 0.0019338784790932095 1104 54-55 0.14740090401738115 1104 56-57 0.31415133986700283 1104 58-59 0.10935700252963443 1104 60-61 0.15110291095422923 1104 62-63 0.04881152921231546 1104 64-65 -0.03268898831662881 1104 66-67 -0.07915877435259944 1104 68-69 -0.02763439127040357 1104 70-71 0.09830009802946549 1104 72-73 0.24253944746575584 1104 74-75 0.2718670605517275 1104 76-77 0.2593515991411266 1104 78-79 0.42930465590065836 1104 80-81 0.4871950278233932 1104 82-83 0.4859102946120508 1104 84-85 0.5419548348940637 1104 86-87 0.5387288165410595 1104 88-89 0.6393663312862543 1104 90-91 0.6336580733715351 1104 92-93 1.0987241747015197 1104 94-95 0.9943115784944894 1104 96-97 1.3240133493521036 1104 98-99 1.4615260553281466 1104 100-101 1.5944710681259338 1105 1 0.14949083591658763 1105 2 0.1708623537885714 1105 3 0.2000509967368771 1105 4 0.1395244733715444 1105 5 0.10942330194109218 1105 6 0.13377976681296389 1105 7 0.12715388052973253 1105 8 0.06660556829933029 1105 9 0.07551778468739201 1105 10-11 0.13431275818729205 1105 12-13 0.21183788371856593 1105 14-15 0.26707292960082896 1105 16-17 0.1776699247488125 1105 18-19 0.21253533905409228 1105 20-21 0.187855941030584 1105 22-23 0.2749038122827443 1105 24-25 0.21628549247638063 1105 26-27 0.22606091562793296 1105 28-29 0.18664665099917244 1105 30-31 0.20536150347464854 1105 32-33 0.21039615458558814 1105 34-35 0.20679813187962992 1105 36-37 0.33029745688581613 1105 38-39 0.3613155511211019 1105 40-41 0.3898407154561383 1105 42-43 0.28104141515204617 1105 44-45 0.3855804696820897 1105 46-47 0.36733080349345926 1105 48-49 0.31897585443604015 1105 50-51 0.4152050246104011 1105 52-53 0.3287999611702901 1105 54-55 0.43764120739146506 1105 56-57 0.5263003189677207 1105 58-59 0.6124346038706108 1105 60-61 0.49066379222905 1105 62-63 0.5935326108476104 1105 64-65 0.5483380801438997 1105 66-67 0.7158791719638131 1105 68-69 0.9058041921376372 1105 70-71 1.0496719945972757 1105 72-73 0.791812802612224 1105 74-75 0.7182012336945256 1105 76-77 0.706124755007572 1105 78-79 1.0592299419477733 1105 80-81 1.1279607693396336 1105 82-83 1.0815609278314078 1105 84-85 1.2053136420699566 1105 86-87 1.414310648096361 1105 88-89 1.5759157608542758 1105 90-91 1.4439385803096947 1105 92-93 1.6500139414551143 1105 94-95 1.7203455584773266 1105 96-97 1.9309647654491684 1105 98-99 2.1472363365528686 1105 100-101 2.709853068096468 1106 1 0.09211139360820653 1106 2 0.08178224251376065 1106 3 0.1384901399567795 1106 4 0.1210395595526279 1106 5 0.08767440690354533 1106 6 -0.00718491930582843 1106 7 -0.0458120453030304 1106 8 0.010466214268163299 1106 9 0.08039541533647565 1106 10-11 0.08657572278058012 1106 12-13 0.07316060462122209 1106 14-15 0.05315414583808575 1106 16-17 0.05581805709277887 1106 18-19 0.06718261946900128 1106 20-21 -0.026455867993583126 1106 22-23 0.12279835724184096 1106 24-25 0.19129320325770038 1106 26-27 0.15552092886353108 1106 28-29 0.13627428763721383 1106 30-31 0.1911137762835864 1106 32-33 0.20775670922283496 1106 34-35 0.03743662200383824 1106 36-37 0.16502546843251764 1106 38-39 0.28602912928297286 1106 40-41 0.3439654968592265 1106 42-43 0.3484886674188701 1106 44-45 0.24760689295757032 1106 46-47 0.08510591369025633 1106 48-49 0.34084003688359843 1106 50-51 0.22899994926932976 1106 52-53 0.19850853106740374 1106 54-55 0.3106775783234639 1106 56-57 0.5029325341629018 1106 58-59 0.5394449467982554 1106 60-61 0.6043938864353251 1106 62-63 0.5116830414535727 1106 64-65 0.613898624001024 1106 66-67 0.6158368375285121 1106 68-69 0.3101592489129388 1106 70-71 0.5231143419088369 1106 72-73 0.4913111152237697 1106 74-75 0.7117333479757875 1106 76-77 0.8450750930841586 1106 78-79 1.051556965915573 1106 80-81 1.1578905787710418 1106 82-83 1.0324391334203007 1106 84-85 1.0694908492998252 1106 86-87 1.2036311411072482 1106 88-89 1.5025547884873163 1106 90-91 1.395497468220384 1106 92-93 1.414499916816549 1106 94-95 1.6307320557763134 1106 96-97 1.6234524431714483 1106 98-99 1.8280277696502374 1106 100-101 2.0292353147336684 1107 1 0.11539570733369686 1107 2 0.07187018945724333 1107 3 0.20066574549311156 1107 4 0.12594152033693717 1107 5 0.06662481059443337 1107 6 0.07798664932162325 1107 7 0.0698526029091937 1107 8 0.10652301934313613 1107 9 0.013101586040050961 1107 10-11 0.006107873876317171 1107 12-13 0.0861363831679327 1107 14-15 0.14477755990959906 1107 16-17 0.1470630109566713 1107 18-19 0.13242195049553374 1107 20-21 0.0773503603801231 1107 22-23 0.1141658601253468 1107 24-25 0.07959337626807894 1107 26-27 0.15117764743331463 1107 28-29 0.09006609847919833 1107 30-31 0.0607792895477175 1107 32-33 0.11597109791948412 1107 34-35 0.07872482846289586 1107 36-37 0.05379421122376016 1107 38-39 0.1642730739196523 1107 40-41 0.22983271139209904 1107 42-43 0.24053739867607504 1107 44-45 0.33497713517210315 1107 46-47 0.2724437164584188 1107 48-49 0.2628411902861387 1107 50-51 0.3251829077468358 1107 52-53 0.36065169715736545 1107 54-55 0.3392963787848231 1107 56-57 0.4669247486957744 1107 58-59 0.5223601717117532 1107 60-61 0.5553202128482511 1107 62-63 0.666959858062576 1107 64-65 0.5667352733666533 1107 66-67 0.7318078582897627 1107 68-69 0.8466896699048689 1107 70-71 0.929220166591648 1107 72-73 0.7938449964233136 1107 74-75 0.8127101357497217 1107 76-77 0.7982361369134559 1107 78-79 0.9981760547275691 1107 80-81 0.9762296791170648 1107 82-83 0.9697589142738146 1107 84-85 1.0394302956666088 1107 86-87 1.2912897339561518 1107 88-89 1.3989648230893934 1107 90-91 1.5608845789470287 1107 92-93 1.5176393343482637 1107 94-95 1.5145988377832325 1107 96-97 1.578938313990367 1107 98-99 1.789623069534894 1107 100-101 2.3554139479516607 1108 1 -0.038706710332398586 1108 2 -0.022949577398669874 1108 3 0.03823195415819214 1108 4 0.040847288469436194 1108 5 -0.011269053975951238 1108 6 0.029062225183032808 1108 7 -0.05628820178504412 1108 8 -4.8586073659606654E-4 1108 9 -0.08501601622644017 1108 10-11 -0.06078947329784512 1108 12-13 -0.043487025304536076 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3068.0 13 4462.0 14 6349.0 15 8679.0 16 11399.0 17 15455.0 18 16300.0 19 12248.0 20 10917.0 21 10997.0 22 11650.0 23 12754.0 24 14099.0 25 16314.0 26 19060.0 27 22710.0 28 27540.0 29 33334.0 30 40617.0 31 49676.0 32 62220.0 33 81713.0 34 113088.0 35 170180.0 36 251704.0 37 345543.0 38 224614.0 39 15315.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.51371982337869 18.78036529284916 14.982623660277403 22.72329122349474 2 15.890002474634993 19.974758723088346 39.895446671615936 24.239792130660728 3 19.731241864999827 23.993363324573515 29.762161051969972 26.51323375845669 4 13.071350009835664 16.825233241327606 36.09527851012578 34.00813823871094 5 13.703728905824713 37.21507398426288 34.00053199386351 15.080665116048895 6 32.530952977455286 35.796766600786235 16.80847970851775 14.86380071324073 7 28.755027632648467 32.9984052615235 20.001534116144963 18.245032989683068 8 26.872456800402826 34.7421014669367 19.064238639189494 19.321203093470977 9 26.398674721601225 16.78118926168186 19.92866359925917 36.89147241745775 10-11 25.36973436284074 26.63868966686173 26.420542062619155 21.571033907678373 12-13 26.31225696940304 23.838587474931426 27.25688279971248 22.59227275595305 14-15 22.829071006070894 25.271810739555328 25.860527810185296 26.03859044418848 16-17 22.059273830622338 28.56949766944107 26.766322418213928 22.604906081722664 18-19 22.04398088293333 26.619358566244745 29.574820947613645 21.761839603208283 20-21 22.51485556420214 25.687877884354858 30.12549441222938 21.671772139213616 22-23 22.2684072286563 25.664649633853486 30.071924066199585 21.995019071290624 24-25 22.153938563884243 25.911560548108586 30.123764259613584 21.810736628393588 26-27 21.655957555414624 26.314683908490466 29.903633466874975 22.125725069219943 28-29 21.759194535550225 26.566136198020736 29.831097925345084 21.843571341083955 30-31 21.845975310001357 26.18839137665625 29.75811991533903 22.20751339800336 32-33 21.550496502337136 26.20887122117552 29.761560103630792 22.47907217285655 34-35 22.254885917156027 26.343814760037333 29.553762644206365 21.847536678600274 36-37 22.30734753305179 25.93906178421043 29.605017939107565 22.14857274363022 38-39 21.842072047211452 26.22746436433699 29.536145495224037 22.394318093227522 40-41 22.234387336126737 26.110947488782244 29.6657319569258 21.98893321816521 42-43 21.971138988166224 26.183106900576547 29.398238591665283 22.44751551959195 44-45 21.93885173706884 26.433704700307565 29.51960680901662 22.10783675360698 46-47 22.41963416071281 26.66978858103115 28.85465436912843 22.0559228891276 48-49 22.1357287034282 26.794996545139288 28.670457012810513 22.398817738622 50-51 22.334999004061697 26.946069631552316 28.606317341822106 22.11261402256388 52-53 22.593231578543655 26.75254211705554 28.47780418492038 22.176422119480428 54-55 22.16380277699766 27.05385984859263 28.485938712060012 22.2963986623497 56-57 22.170298026804968 27.130596585601825 28.349043589930755 22.350061797662452 58-59 22.128944936365112 26.748985194542012 28.4444619025762 22.67760796651668 60-61 21.9526143689772 26.957018766941776 28.69431251183063 22.39605435225039 62-63 22.027464390566177 26.96608118691102 28.823537053235064 22.182917369287736 64-65 21.802017362730627 27.06644826131442 28.590481304196675 22.54105307175828 66-67 21.701580267258542 27.136045508925733 28.45551237750238 22.70686184631335 68-69 22.178233133578196 26.772178619756424 28.580738449642375 22.468849797023005 70-71 22.090622962501996 26.90456189230752 28.22260272701517 22.78221241817532 72-73 22.02072170267097 27.058066296086892 28.10788424351372 22.81332775772842 74-75 21.974419850473165 27.380014178202437 27.96730847982699 22.67825749149741 76-77 22.060435515778508 27.011640724845137 28.232933267184883 22.694990492191472 78-79 22.069157708376892 27.305164156613465 28.126998835803796 22.498679299205847 80-81 22.21581974585571 27.22902060050898 27.961098924755127 22.59406072888018 82-83 22.146388688273348 27.263915029051024 28.059218614922806 22.530477667752827 84-85 21.969388831374424 27.426482437535626 28.004551805241135 22.599576925848815 86-87 22.179480438339557 27.29430795735375 27.81810702388722 22.708104580419473 88-89 22.099815936935435 27.45889356168989 27.615954936561494 22.82533556481318 90-91 22.041221584186896 27.38023176508615 27.717254636925592 22.86129201380136 92-93 22.1138366283578 27.089793192739986 27.8250611563428 22.971309022559414 94-95 21.846174402567915 26.843500522249496 28.094517725707817 23.215807349474765 96-97 22.09150266150758 26.73158827513895 27.981491619576076 23.19541744377739 98-99 21.85956700150226 26.920051751724106 27.655252852577178 23.565128394196456 100-101 22.05942667952562 26.416057278846505 28.035252422962266 23.48926361866561 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1724.0 1 1151.0 2 950.5 3 1261.5 4 908.5 5 739.0 6 1478.5 7 3176.0 8 4889.0 9 5393.0 10 4153.5 11 2684.5 12 2332.0 13 2391.0 14 2440.0 15 2423.5 16 2531.5 17 2638.5 18 2708.5 19 2858.5 20 3147.0 21 3706.5 22 4419.5 23 5364.5 24 6603.5 25 7952.0 26 9645.5 27 11853.0 28 13656.0 29 15416.0 30 17788.0 31 20834.0 32 25244.0 33 27911.0 34 28760.5 35 30528.0 36 33479.5 37 36767.0 38 39785.5 39 42192.0 40 44251.0 41 46881.0 42 48669.0 43 50326.0 44 52721.0 45 56082.0 46 57360.5 47 58018.5 48 59542.5 49 59336.0 50 58309.0 51 56777.0 52 56173.5 53 56449.5 54 56910.0 55 53762.0 56 49011.5 57 45487.5 58 42600.5 59 37988.0 60 30286.0 61 24790.0 62 20908.0 63 15592.5 64 10917.5 65 8423.5 66 6429.0 67 4997.0 68 3937.5 69 3020.5 70 2262.5 71 1488.0 72 1076.0 73 843.5 74 629.0 75 460.5 76 318.5 77 210.0 78 127.0 79 69.5 80 41.0 81 27.0 82 10.5 83 8.0 84 5.0 85 2.5 86 1.5 87 0.5 88 2.0 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010268680647743427 3 0.005814795065589651 4 0.0 5 3.7115713184614793E-4 6 6.1859521974358E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.1859521974358E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0015464880493589496 26-27 0.021094096993256074 28-29 4.94876175794864E-4 30-31 3.0929760987179E-5 32-33 0.003587852274512763 34-35 0.016238124518268972 36-37 0.007763370007781927 38-39 0.02152711364707658 40-41 0.023382899306307322 42-43 0.01871250539724329 44-45 0.009309858057140878 46-47 0.0022578725520640665 48-49 1.8557856592307396E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.47438087897432E-4 68-69 2.1650832691025296E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.47438087897432E-4 82-83 8.351035466538328E-4 84-85 0.003989939167346091 86-87 6.804547417179379E-4 88-89 3.0929760987179E-5 90-91 0.0036187820354999425 92-93 7.732440246794748E-4 94-95 9.278928296153698E-5 96-97 6.1859521974358E-5 98-99 9.278928296153698E-5 100-101 0.0032785546646409737 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1616566.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.78787433010698 #Duplication Level Percentage of deduplicated Percentage of total 1 79.41949607222995 45.894838583824495 2 12.040549245697289 13.915954933516387 3 3.5521779239813673 6.158184344076467 4 1.5429548002272766 3.566563123702768 5 0.8718735306256614 2.5191859009771207 6 0.5165380656213633 1.7909782093706341 7 0.3564430239235255 1.4418659270635206 8 0.24827982248986452 1.147805054459645 9 0.2000996517679822 1.040700017687969 >10 1.1435442559761964 12.02617079043613 >50 0.06818746602636183 2.7035135716899155 >100 0.03471365108648837 3.668727236996941 >500 0.00332119168220894 1.3492919022942669 >1k 0.0014998930177717795 1.2772273077145417 >5k 2.1427043111025418E-4 0.8728854974051055 >10k+ 1.0713521555512709E-4 0.6261075987841571 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10113 0.6255853457266823 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8615 0.5329197818090942 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5484 0.3392376185073792 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2150 0.13299797224486967 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1816 0.11233689190543411 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1757 0.10868718010894698 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1707 0.10559420401022909 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.185952197435799E-5 2 0.0 0.0 0.0 0.0 6.185952197435799E-5 3 0.0 0.0 0.0 0.0 6.185952197435799E-5 4 0.0 0.0 0.0 0.0 6.185952197435799E-5 5 0.0 0.0 0.0 0.0 6.185952197435799E-5 6 0.0 0.0 0.0 0.0 1.2371904394871598E-4 7 0.0 0.0 0.0 0.0 1.2371904394871598E-4 8 0.0 0.0 0.0 0.0 1.2371904394871598E-4 9 0.0 0.0 0.0 0.0 1.2371904394871598E-4 10-11 0.0 0.0 0.0 0.0 1.2371904394871598E-4 12-13 6.185952197435799E-5 0.0 0.0 0.0 1.2371904394871598E-4 14-15 1.2371904394871598E-4 0.0 0.0 0.0 2.1650832691025296E-4 16-17 1.5464880493589497E-4 0.0 0.0 3.0929760987178994E-5 2.4743808789743195E-4 18-19 1.8557856592307396E-4 0.0 0.0 6.185952197435799E-5 2.4743808789743195E-4 20-21 1.8557856592307396E-4 0.0 0.0 1.2371904394871598E-4 2.7836784888461095E-4 22-23 1.8557856592307396E-4 0.0 0.0 4.330166538205059E-4 3.7115713184614793E-4 24-25 2.4743808789743195E-4 0.0 0.0 7.113845027051169E-4 3.7115713184614793E-4 26-27 2.4743808789743195E-4 0.0 0.0 0.0017629963762692026 4.639464148076849E-4 28-29 2.7836784888461095E-4 0.0 0.0 0.004330166538205059 4.948761757948639E-4 30-31 3.0929760987178994E-4 0.0 0.0 0.01234097463388442 4.948761757948639E-4 32-33 3.0929760987178994E-4 0.0 0.0 0.02771306584451238 4.948761757948639E-4 34-35 3.7115713184614793E-4 0.0 0.0 0.05171456037056328 4.948761757948639E-4 36-37 4.330166538205059E-4 0.0 0.0 0.09136651395612674 4.948761757948639E-4 38-39 4.330166538205059E-4 0.0 0.0 0.15140118003224118 4.948761757948639E-4 40-41 4.330166538205059E-4 0.0 0.0 0.21666297571518886 4.948761757948639E-4 42-43 4.639464148076849E-4 0.0 0.0 0.2789245845823802 4.948761757948639E-4 44-45 4.948761757948639E-4 0.0 0.0 0.35448599067405845 5.258059367820428E-4 46-47 4.948761757948639E-4 0.0 0.0 0.44671853793782623 5.567356977692219E-4 48-49 4.948761757948639E-4 0.0 0.0 0.5435286898276965 6.185952197435799E-4 50-51 4.948761757948639E-4 0.0 0.0 0.6503910140383999 6.185952197435799E-4 52-53 4.948761757948639E-4 0.0 0.0 0.7487167242166419 6.185952197435799E-4 54-55 4.948761757948639E-4 0.0 0.0 0.8547130151197043 6.185952197435799E-4 56-57 4.948761757948639E-4 0.0 0.0 0.9792362328540869 6.185952197435799E-4 58-59 4.948761757948639E-4 0.0 0.0 1.1044089755692004 6.185952197435799E-4 60-61 4.948761757948639E-4 0.0 0.0 1.239664820366134 6.185952197435799E-4 62-63 4.948761757948639E-4 0.0 0.0 1.3684872748777348 6.185952197435799E-4 64-65 4.948761757948639E-4 0.0 0.0 1.5026915078011043 6.185952197435799E-4 66-67 4.948761757948639E-4 0.0 0.0 1.6568145068002171 6.185952197435799E-4 68-69 4.948761757948639E-4 0.0 0.0 1.8306706933091503 6.185952197435799E-4 70-71 4.948761757948639E-4 0.0 0.0 2.0079291535266734 6.804547417179379E-4 72-73 5.258059367820428E-4 0.0 0.0 2.1857443494419653 7.732440246794748E-4 74-75 5.567356977692219E-4 0.0 0.0 2.367549484524603 8.041737856666538E-4 76-77 5.567356977692219E-4 0.0 0.0 2.56011817643078 8.041737856666538E-4 78-79 5.567356977692219E-4 0.0 0.0 2.7866786756618662 8.041737856666538E-4 80-81 5.567356977692219E-4 0.0 0.0 3.021219053227644 8.351035466538329E-4 82-83 5.567356977692219E-4 0.0 0.0 3.2657188138312945 8.660333076410118E-4 84-85 5.567356977692219E-4 0.0 0.0 3.5067853709653676 8.660333076410118E-4 86-87 5.567356977692219E-4 0.0 0.0 3.756233893326966 9.278928296153698E-4 88-89 5.567356977692219E-4 0.0 0.0 4.024704218695679 0.0010825416345512648 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4465 0.0 49.47395 1 GTATCAA 7240 0.0 38.647537 1 TATCAAC 9115 0.0 30.489079 2 ATCAACG 9085 0.0 30.42724 3 TCAACGC 9220 0.0 30.134401 4 CAACGCA 9355 0.0 29.699537 5 AACGCAG 9640 0.0 28.821491 6 ACGCAGA 10925 0.0 25.431503 7 CGCAGAG 11155 0.0 24.82199 8 TGGTATC 2150 0.0 23.863295 2 GTGGTAT 2310 0.0 23.64999 1 GCAGAGT 12195 0.0 22.354641 9 GAGTACT 7670 0.0 22.29178 12-13 TACATGG 6225 0.0 22.283768 2 GTACATG 6465 0.0 21.970894 1 GTACTTT 7970 0.0 21.244122 14-15 CAGAGTA 11470 0.0 21.1797 10-11 ACATGGG 6250 0.0 21.050589 3 GACCGCG 160 4.995856E-5 20.77857 7 AGTACTT 7920 0.0 20.538702 12-13 >>END_MODULE