FastQCFastQC Report
Thu 26 May 2016
SRR937973_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937973_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618462
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104900.6481462030001323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87010.537609162278756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53900.3330322244204683No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21400.13222429689421192No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18150.11214350414158628No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16940.10466727053214718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43350.050.5630841
GTATCAA72800.040.4072531
ATCAACG92250.031.9894983
TATCAAC92200.031.9037672
TCAACGC93250.031.7993324
CAACGCA94650.031.3289765
AACGCAG98600.030.17036
ACGCAGA110750.026.6161447
CGCAGAG111650.026.3590138
TAAGGTA5750.025.6198224
GTACATG64700.025.094851
TACATGG63850.024.560392
GTCCTAC3800.023.8770121
TGGTATC18850.023.6972922
GCAGAGT122600.023.6169629
GAGTACT76600.022.83752612-13
ACATGGG66300.022.0759493
GTACTTT81650.021.44154214-15
CAGAGTA116900.021.33117310-11
GTGGTAT23800.020.2653621
Produced by FastQC (version 0.11.5)