##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937973_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1618462 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.892845800519257 33.0 31.0 34.0 30.0 34.0 2 32.07530729791617 34.0 31.0 34.0 30.0 34.0 3 32.138327622149916 34.0 31.0 34.0 30.0 34.0 4 35.66240912668941 37.0 35.0 37.0 35.0 37.0 5 35.68130546160491 37.0 35.0 37.0 35.0 37.0 6 35.71245787667551 37.0 36.0 37.0 35.0 37.0 7 35.652830897481685 37.0 35.0 37.0 33.0 37.0 8 35.63852534072471 37.0 35.0 37.0 33.0 37.0 9 37.29895048509017 39.0 37.0 39.0 34.0 39.0 10-11 37.224097630960756 39.0 37.0 39.0 34.0 39.0 12-13 37.122777365177555 39.0 37.0 39.0 33.5 39.0 14-15 38.36836916776545 40.0 38.0 41.0 33.5 41.0 16-17 38.28770215179596 40.0 38.0 41.0 33.0 41.0 18-19 38.239885768093416 40.0 38.0 41.0 33.5 41.0 20-21 38.14263078156917 40.0 38.0 41.0 33.5 41.0 22-23 37.99202977888885 40.0 38.0 41.0 33.0 41.0 24-25 37.93399288954575 40.0 38.0 41.0 33.0 41.0 26-27 37.93507076471366 40.0 38.0 41.0 33.0 41.0 28-29 37.83666344962069 40.0 38.0 41.0 33.0 41.0 30-31 37.632252410004064 40.0 37.5 41.0 32.5 41.0 32-33 37.39080744558723 40.0 37.0 41.0 32.0 41.0 34-35 37.17534733592757 40.0 36.0 41.0 31.0 41.0 36-37 36.9469851624567 40.0 36.0 41.0 31.0 41.0 38-39 36.70353459024679 39.5 36.0 41.0 30.0 41.0 40-41 36.46991650097438 39.0 35.0 41.0 30.0 41.0 42-43 36.178377682021576 39.0 35.0 41.0 29.5 41.0 44-45 35.93242720558159 39.0 35.0 41.0 29.0 41.0 46-47 35.75960912273504 39.0 35.0 41.0 27.5 41.0 48-49 35.477148366782785 39.0 35.0 41.0 26.5 41.0 50-51 34.82183671905797 38.5 34.5 40.0 25.0 40.5 52-53 34.88999649049529 38.5 34.5 40.0 26.0 41.0 54-55 35.02305120540365 39.0 35.0 40.0 25.5 41.0 56-57 34.80824572958772 38.5 35.0 40.0 25.0 41.0 58-59 34.54536220189291 38.0 34.0 40.0 24.0 41.0 60-61 34.16771787042266 38.0 34.0 40.0 23.0 41.0 62-63 33.75128084564234 37.0 34.0 40.0 22.0 41.0 64-65 33.32596625685373 36.5 33.0 39.5 20.5 41.0 66-67 32.894932967224435 36.0 33.0 39.0 18.5 41.0 68-69 32.405646533560876 35.5 32.0 39.0 17.0 40.5 70-71 31.919332057224697 35.0 32.0 38.5 12.5 40.0 72-73 31.43734638193544 35.0 31.0 37.5 8.0 40.0 74-75 30.92055111581242 35.0 31.0 37.0 6.5 39.0 76-77 30.389161129516786 35.0 30.0 36.0 2.0 39.0 78-79 29.829135005950093 34.0 29.5 36.0 2.0 38.5 80-81 29.306545658779754 34.0 29.0 35.0 2.0 37.0 82-83 28.78054350364729 34.0 29.0 35.0 2.0 37.0 84-85 28.29187710307687 34.0 27.5 35.0 2.0 36.0 86-87 27.798378336964355 34.0 26.5 35.0 2.0 36.0 88-89 27.248124762892175 33.0 26.0 35.0 2.0 35.5 90-91 26.64479023912826 33.0 25.0 35.0 2.0 35.0 92-93 26.040624370544382 32.5 23.5 35.0 2.0 35.0 94-95 25.450326915306015 32.0 21.0 35.0 2.0 35.0 96-97 24.63291476722963 32.0 12.5 34.0 2.0 35.0 98-99 23.580068608345456 31.0 2.0 34.0 2.0 35.0 100-101 20.761305177384454 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3238538864978935 1101 2 0.306404096126073 1101 3 0.2751687330781323 1101 4 0.20264201630492096 1101 5 0.1439530598544181 1101 6 0.14921098066754013 1101 7 0.26054667367792206 1101 8 0.17838967616361145 1101 9 0.1630885358952625 1101 10-11 0.2732120480255773 1101 12-13 0.07333072079092773 1101 14-15 0.2444871720243853 1101 16-17 0.3358579635556751 1101 18-19 0.3065908325202713 1101 20-21 0.3973372094662153 1101 22-23 0.23154608032766788 1101 24-25 0.2844016400824785 1101 26-27 0.18586111816727424 1101 28-29 0.2588247362024134 1101 30-31 0.45044701464445325 1101 32-33 0.39041944906055903 1101 34-35 0.40315512068713844 1101 36-37 0.357656737555466 1101 38-39 0.5478329563031821 1101 40-41 0.47233422962995064 1101 42-43 0.46635454271592636 1101 44-45 0.35498711873538014 1101 46-47 0.3338142782733229 1101 48-49 0.4261859636889369 1101 50-51 0.42184753021220445 1101 52-53 0.48785967136523567 1101 54-55 0.4912098747436744 1101 56-57 0.37529841962950883 1101 58-59 0.39689018176030544 1101 60-61 0.46119020249758336 1101 62-63 0.5210576709920431 1101 64-65 0.48776380054378876 1101 66-67 0.4469142393792609 1101 68-69 0.575284517278206 1101 70-71 0.5407105631420706 1101 72-73 0.525260522603002 1101 74-75 0.6802840331281672 1101 76-77 0.6638536066584955 1101 78-79 0.5893767136929142 1101 80-81 0.5965205539326384 1101 82-83 0.5534240682380478 1101 84-85 0.41052358452870763 1101 86-87 0.6679852932177859 1101 88-89 0.6804847417841415 1101 90-91 0.7083254553672553 1101 92-93 1.0200457831337495 1101 94-95 1.078553495424245 1101 96-97 1.1412044355005797 1101 98-99 1.2433902570835222 1101 100-101 1.2209150514354477 1102 1 0.42080166989790513 1102 2 0.19300898069223393 1102 3 0.2846707489560316 1102 4 0.21069831371700332 1102 5 0.14101833793879592 1102 6 0.24760544442190024 1102 7 0.044282389032019864 1102 8 0.08055310775866786 1102 9 0.12978314542382208 1102 10-11 0.278278555403503 1102 12-13 0.2163527244720811 1102 14-15 0.30818703779283396 1102 16-17 0.24613516540269842 1102 18-19 0.1883639105106738 1102 20-21 0.26435034838264215 1102 22-23 0.39186404220129134 1102 24-25 0.3641859654355031 1102 26-27 0.33857583212807896 1102 28-29 0.41476321958314344 1102 30-31 0.32903335592346394 1102 32-33 0.3632850544756039 1102 34-35 0.4020390895161867 1102 36-37 0.4040786805414385 1102 38-39 0.395551332654577 1102 40-41 0.5289221320540491 1102 42-43 0.4918472340463751 1102 44-45 0.5328768603384546 1102 46-47 0.4403165004763139 1102 48-49 0.47549934360653623 1102 50-51 0.5904721209887498 1102 52-53 0.5160069345759339 1102 54-55 0.6952016640631555 1102 56-57 0.5373153171253691 1102 58-59 0.495110784162236 1102 60-61 0.5399094767719248 1102 62-63 0.6659810878317245 1102 64-65 0.6202252300159543 1102 66-67 0.585654877197932 1102 68-69 0.418807135846599 1102 70-71 0.5070980897563189 1102 72-73 0.6647805306059134 1102 74-75 0.6826728833010414 1102 76-77 0.7458120405692981 1102 78-79 0.5334493882191715 1102 80-81 0.8472793637494824 1102 82-83 0.9525700555036067 1102 84-85 0.6693591318889709 1102 86-87 0.6197242193558061 1102 88-89 0.6699330650440949 1102 90-91 0.8268904811963687 1102 92-93 0.828400795029907 1102 94-95 0.9002864530439147 1102 96-97 1.0132922578474641 1102 98-99 1.1434632644232146 1102 100-101 0.877896221986461 1103 1 0.3699119514238802 1103 2 0.2730644099561985 1103 3 0.1616562497543299 1103 4 0.04549825161398502 1103 5 -0.05727939043463692 1103 6 0.07196048832270918 1103 7 0.1335026485206896 1103 8 0.1719478999105135 1103 9 0.23210575208229045 1103 10-11 0.232579305506043 1103 12-13 0.0682738629038866 1103 14-15 0.09349295310563122 1103 16-17 0.21933423571119448 1103 18-19 0.21793699858341853 1103 20-21 0.15186995833858674 1103 22-23 0.25180527605856184 1103 24-25 0.2645680843998619 1103 26-27 0.3322431777568866 1103 28-29 0.39387968823849917 1103 30-31 0.1647917195551969 1103 32-33 0.257911984477623 1103 34-35 0.39695475222261933 1103 36-37 0.4264770158097022 1103 38-39 0.3972262604134684 1103 40-41 0.4346237919195133 1103 42-43 0.2805023096938797 1103 44-45 0.40850976659764626 1103 46-47 0.424030909999388 1103 48-49 0.5337839561925861 1103 50-51 0.4070517739070141 1103 52-53 0.6286766461444699 1103 54-55 0.7422231521720519 1103 56-57 0.47628310929344053 1103 58-59 0.5601771393868873 1103 60-61 0.6112505544447302 1103 62-63 0.6595939774528716 1103 64-65 0.5960034295381362 1103 66-67 0.506567374504094 1103 68-69 0.4896546335351175 1103 70-71 0.6583015675255268 1103 72-73 0.5937949232128794 1103 74-75 0.5811543717343532 1103 76-77 0.43053933183478676 1103 78-79 0.4079206435575955 1103 80-81 0.35513817670120673 1103 82-83 0.42143118341497754 1103 84-85 0.5589703159943049 1103 86-87 0.6302240328150113 1103 88-89 0.37265169432845013 1103 90-91 0.43176742220544995 1103 92-93 0.3760650957568359 1103 94-95 0.4228391189551495 1103 96-97 0.5516040289190407 1103 98-99 0.6950134068198857 1103 100-101 0.5470569738037838 1104 1 0.3001280913174398 1104 2 0.09388769658593787 1104 3 0.0643759195707787 1104 4 0.1436974478776989 1104 5 0.1260224451252654 1104 6 0.11246879721811354 1104 7 0.20155521721842007 1104 8 0.1511571971843253 1104 9 0.1048851590552431 1104 10-11 0.17337435275270252 1104 12-13 0.026644667726309024 1104 14-15 0.18969692834186702 1104 16-17 0.3129488767451889 1104 18-19 0.26501422243483574 1104 20-21 0.23594837693313053 1104 22-23 0.11195950653537068 1104 24-25 0.20798722201255515 1104 26-27 0.17631399344059417 1104 28-29 0.3019876495722684 1104 30-31 0.3279610472406489 1104 32-33 0.3941224764945659 1104 34-35 0.5696222877933863 1104 36-37 0.35757400722927457 1104 38-39 0.36610102961081736 1104 40-41 0.4082123658306642 1104 42-43 0.38589603186179744 1104 44-45 0.21414786804220398 1104 46-47 0.12176089805196 1104 48-49 0.4098260980411865 1104 50-51 0.1433393811623631 1104 52-53 0.29668251124710565 1104 54-55 0.38408695145520255 1104 56-57 0.3745757127473368 1104 58-59 0.3949995571125271 1104 60-61 0.41707329800940585 1104 62-63 0.524115537135728 1104 64-65 0.5128960112694259 1104 66-67 0.4796335198283046 1104 68-69 0.44608995448655975 1104 70-71 0.6314046412737611 1104 72-73 0.6993950101032844 1104 74-75 0.5472649397804048 1104 76-77 0.7376274169873902 1104 78-79 0.7095020490099699 1104 80-81 0.7784693377743039 1104 82-83 0.7874309661889534 1104 84-85 0.8472531442771327 1104 86-87 0.7118803812455567 1104 88-89 0.6881105843034696 1104 90-91 0.7134932829635687 1104 92-93 0.7344956377357654 1104 94-95 0.7051775571000398 1104 96-97 0.8645648196217692 1104 98-99 0.8595023825991817 1104 100-101 0.7365204255252991 1105 1 0.37339882936422697 1105 2 0.2959638183646618 1105 3 0.3432254289499923 1105 4 0.24943055823211324 1105 5 0.2762878034698488 1105 6 0.12730468607789902 1105 7 0.22160961870136475 1105 8 0.28313640372862636 1105 9 0.41773720717033314 1105 10-11 0.4002768935034453 1105 12-13 0.221842024217203 1105 14-15 0.24870339607372927 1105 16-17 0.28329341947050324 1105 18-19 0.254798429885831 1105 20-21 0.34736603928126186 1105 22-23 0.31675247866972 1105 24-25 0.38046450435624735 1105 26-27 0.4122838509893043 1105 28-29 0.4495113746634374 1105 30-31 0.4843068794825953 1105 32-33 0.3918825458060837 1105 34-35 0.6230838340044258 1105 36-37 0.5887817201506351 1105 38-39 0.47628438275704355 1105 40-41 0.5244544322810754 1105 42-43 0.5502211512428303 1105 44-45 0.6223639023755823 1105 46-47 0.624317767750334 1105 48-49 0.6561245808442706 1105 50-51 0.6901730133680388 1105 52-53 0.678611346047937 1105 54-55 0.6909149420256071 1105 56-57 0.810588688508453 1105 58-59 0.7906347447976501 1105 60-61 0.7250153100583319 1105 62-63 0.7170866640523741 1105 64-65 0.7467581135845123 1105 66-67 0.7313479724889405 1105 68-69 0.7027480915380053 1105 70-71 0.8434015786717595 1105 72-73 0.7967055619761076 1105 74-75 0.7683275820805804 1105 76-77 0.8291069358364958 1105 78-79 0.8573737421050112 1105 80-81 0.9942814310224755 1105 82-83 0.9355162354762925 1105 84-85 0.886503612734348 1105 86-87 1.1020331083717458 1105 88-89 1.009984211673462 1105 90-91 0.8082274209015523 1105 92-93 0.8841015292148775 1105 94-95 1.2192399374671474 1105 96-97 1.1868978372921752 1105 98-99 1.1224156827543261 1105 100-101 0.9115890318399025 1106 1 0.09482021004000529 1106 2 0.11969173186442106 1106 3 0.017079395065117353 1106 4 0.1158951040333065 1106 5 0.1202562657527082 1106 6 0.17047371043143755 1106 7 0.2660036888868973 1106 8 0.1296220193823885 1106 9 0.11203992527488538 1106 10-11 -0.4740260741330502 1106 12-13 -0.07020644580114066 1106 14-15 0.039191511725540806 1106 16-17 0.15059818607626596 1106 18-19 0.10248472301878309 1106 20-21 0.10734985089712978 1106 22-23 0.03565690092142404 1106 24-25 0.07876246263918318 1106 26-27 -0.08732138169531822 1106 28-29 -0.02945288846485994 1106 30-31 0.03323268350177244 1106 32-33 0.12359692375806475 1106 34-35 -0.08653936497935888 1106 36-37 -0.07011436617494127 1106 38-39 0.17404311812920525 1106 40-41 0.34312074648389057 1106 42-43 0.1374614138439938 1106 44-45 0.17070096699109882 1106 46-47 0.2966692787051741 1106 48-49 0.06147282994887604 1106 50-51 0.1758048477162646 1106 52-53 0.11303142993745752 1106 54-55 0.01257271521824066 1106 56-57 0.050525533535868306 1106 58-59 0.1512224236905979 1106 60-61 -0.004326565064978638 1106 62-63 0.12190056391676762 1106 64-65 0.1028263402936318 1106 66-67 -0.04964077440067882 1106 68-69 -0.30637873384656444 1106 70-71 -0.16082603811893392 1106 72-73 0.07714338914561125 1106 74-75 0.25165904392441973 1106 76-77 0.3604867460518655 1106 78-79 0.45493195517565255 1106 80-81 0.43696959954386827 1106 82-83 0.20753567401384743 1106 84-85 0.3170908471485454 1106 86-87 0.12424476447619526 1106 88-89 0.077539523935382 1106 90-91 0.1303411747117842 1106 92-93 0.18594100844173767 1106 94-95 -0.024648869544076746 1106 96-97 0.2684333710292819 1106 98-99 0.16157036214088194 1106 100-101 -0.05986997357260293 1107 1 0.29479594501016493 1107 2 0.2533542925361587 1107 3 0.19433140609361033 1107 4 0.1935131431403292 1107 5 0.28851482100843384 1107 6 0.22066420191191582 1107 7 0.13304158851764925 1107 8 0.17164817136650612 1107 9 0.16514845153719904 1107 10-11 0.2272868032669777 1107 12-13 0.16964695969682708 1107 14-15 0.3217627325204546 1107 16-17 0.34821889360348024 1107 18-19 0.3800662179645471 1107 20-21 0.3936929642837299 1107 22-23 0.42119065460424565 1107 24-25 0.4853849830793635 1107 26-27 0.46660361517075444 1107 28-29 0.48904369866968267 1107 30-31 0.5745429804514899 1107 32-33 0.5598544864201216 1107 34-35 0.5809410232317944 1107 36-37 0.5933289368600967 1107 38-39 0.6762658241858617 1107 40-41 0.6309432203000114 1107 42-43 0.6708364595700189 1107 44-45 0.7509427082672318 1107 46-47 0.7687254577473794 1107 48-49 0.8076367926408423 1107 50-51 0.6664996486220645 1107 52-53 0.8027918439237922 1107 54-55 0.8709893817016336 1107 56-57 0.8212406115028514 1107 58-59 0.7941397840365596 1107 60-61 0.6267300706876071 1107 62-63 0.6878503125794424 1107 64-65 0.7834419169766207 1107 66-67 0.7535527894284471 1107 68-69 0.7785791102458504 1107 70-71 0.9518516702359321 1107 72-73 0.9987975824140101 1107 74-75 0.903556114746781 1107 76-77 0.863508878544792 1107 78-79 0.7903756496767258 1107 80-81 0.8066816007637136 1107 82-83 0.7188775594873071 1107 84-85 0.6805003550815947 1107 86-87 0.8574491119484904 1107 88-89 0.8269591058877701 1107 90-91 0.9166688933664631 1107 92-93 0.8278938155244866 1107 94-95 0.86521373613893 1107 96-97 0.7939045048073048 1107 98-99 0.8258984957202387 1107 100-101 0.7238102177325985 1108 1 0.4187128135658753 1108 2 0.3059747499466994 1108 3 0.23937393765341142 1108 4 0.23142579307107525 1108 5 0.21897210442423187 1108 6 0.26828298774643855 1108 7 0.2566502548748488 1108 8 0.17638115384639974 1108 9 0.28577645560670817 1108 10-11 0.24761320210810567 1108 12-13 0.23321821573589574 1108 14-15 0.29703096635692816 1108 16-17 0.2903744604670422 1108 18-19 0.3380586362591842 1108 20-21 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1258.0 4 1257.0 5 1397.0 6 1695.0 7 2125.0 8 2583.0 9 3163.0 10 3991.0 11 4885.0 12 6294.0 13 7531.0 14 9313.0 15 11025.0 16 13433.0 17 17743.0 18 16164.0 19 12908.0 20 12098.0 21 12901.0 22 13474.0 23 14571.0 24 15831.0 25 18187.0 26 20466.0 27 23308.0 28 27542.0 29 32659.0 30 38763.0 31 47117.0 32 58836.0 33 76333.0 34 106056.0 35 158855.0 36 232130.0 37 316155.0 38 242604.0 39 29289.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.26782680404177 18.41095043169828 14.616513825273971 22.70470893898598 2 15.629677024508304 19.6440438546873 40.615844932199565 24.110434188604827 3 19.36188273465403 24.17307943173226 29.624656177491243 26.84038165612247 4 12.828477747817876 16.547778553396395 36.41562385808364 34.20811984070209 5 13.612128362960407 37.17539153374369 34.036133775111274 15.176346328184618 6 32.285073807037776 35.70567000361241 17.138362834704896 14.87089335464492 7 28.59602042961552 33.07977690661984 20.207635135970715 18.116567527793922 8 26.67856504781505 34.71127055299035 19.216215263846706 19.3939491353479 9 26.50214884125775 16.9003026676294 20.028610003857526 36.56893848725532 10-11 25.276212389588476 26.611059873354282 26.498828058695693 21.613899678361552 12-13 26.307451876461553 23.97970119008701 27.2391954580052 22.47365147544624 14-15 22.977141899757715 25.272503695515493 25.902611497612675 25.847742907114124 16-17 22.01940932417924 28.592228912925133 26.772013551223754 22.616348211671873 18-19 22.111203334894437 26.62258472866887 29.565007720521752 21.70120421591494 20-21 22.547736513675463 25.67984667179286 30.113107069725192 21.65930974480648 22-23 22.266509084714933 25.677871864604 30.060014486575188 21.99560456410588 24-25 22.11984687503865 25.907061311828222 30.14743534242848 21.825656470704647 26-27 21.668916773658818 26.238514638782856 29.95256689959164 22.140001687966684 28-29 21.739076668904943 26.624448624203772 29.82409767281388 21.812377034077404 30-31 21.851884142386417 26.16884349526762 29.792315526065366 22.1869568362806 32-33 21.537406099069848 26.215000281339524 29.805232667785013 22.44236095180561 34-35 22.304778005259635 26.310348047239003 29.556220756197504 21.82865319130386 36-37 22.349354233959726 25.902798731716487 29.588222127689047 22.159624906634747 38-39 21.853507198663834 26.21829307152365 29.554221548178646 22.373978181633863 40-41 22.267543713242613 26.1246508936751 29.717834310645596 21.88997108243669 42-43 22.006990015676227 26.15361839014524 29.425660305513386 22.413731288665147 44-45 21.874663957312844 26.465207916949407 29.540670589791223 22.119457535946523 46-47 22.423583916775446 26.643681272497133 28.84308497488232 22.0896498358451 48-49 22.18281785582287 26.76970448373344 28.68802001505579 22.3594576453879 50-51 22.298433362515098 26.95857497391101 28.634914474145706 22.108077189428187 52-53 22.576777433667424 26.77737186352958 28.505563277608882 22.14028742519411 54-55 22.14865582700546 27.049772270701673 28.54995196347184 22.251619938821023 56-57 22.145988130719683 27.201325057901755 28.315140053490563 22.337546757888006 58-59 22.178731141275733 26.752752020290664 28.443733452651408 22.624783385782195 60-61 21.973645297133963 26.959196595971918 28.715198191803225 22.351959915090898 62-63 22.093029363951516 26.87288702005749 28.88951306868728 22.14457054730371 64-65 21.872167749275917 26.960971990595034 28.71038943341235 22.4564708267167 66-67 21.77107199634155 27.117257276341057 28.53984973789877 22.57182098941862 68-69 22.241634072679698 26.762809915823162 28.65553743462405 22.340018576873092 70-71 22.167062745501653 26.89716816768646 28.276598092331028 22.65917099448086 72-73 22.042139734752208 27.06353287905611 28.24089756015547 22.653429826036216 74-75 22.03449648434023 27.449620002175656 28.14527314352397 22.370610369960144 76-77 22.29486363383537 27.101608706669055 28.134042408548453 22.469485250947116 78-79 22.242913841395495 27.323133776107206 28.125255877259253 22.30869650523805 80-81 22.439844194656565 27.36774745345522 27.819336993016197 22.373071358872014 82-83 22.3773732294832 27.447730395074228 27.923782260810665 22.251114114631903 84-85 22.27595304139268 27.54478343613086 27.88412188374553 22.295141638730932 86-87 22.551419858554837 27.527644836077474 27.62450603876302 22.29642926660467 88-89 22.481279508649692 27.571973597833033 27.496590935783495 22.450155957733784 90-91 22.443086363693972 27.57467185925967 27.49482940514435 22.48741237190201 92-93 22.550864182727224 27.292944439259355 27.670442991383204 22.485748386630217 94-95 22.4499472966749 27.04641911044206 27.838576361090418 22.665057231792623 96-97 22.765723533351608 26.841792768236346 27.717536604973507 22.674947093438536 98-99 22.51813488796422 27.144107459409746 27.3706081306602 22.967149521965837 100-101 23.05718899334226 26.481714264847778 27.63160907908768 22.82948766272228 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1795.0 1 1202.0 2 993.0 3 1336.0 4 963.5 5 775.5 6 1427.5 7 3020.0 8 4882.0 9 5454.0 10 4166.0 11 2782.0 12 2428.5 13 2371.0 14 2480.5 15 2564.0 16 2570.0 17 2641.0 18 2779.5 19 2981.0 20 3291.5 21 3828.0 22 4555.0 23 5500.5 24 6697.0 25 8183.0 26 9996.5 27 12203.0 28 14004.5 29 15725.0 30 17975.5 31 21038.5 32 25699.5 33 28062.5 34 28526.0 35 30598.5 36 33825.0 37 37122.5 38 39876.5 39 42388.5 40 44641.0 41 47236.5 42 48961.0 43 50260.5 44 52556.0 45 55700.0 46 56977.5 47 57658.0 48 59161.0 49 58935.0 50 57993.5 51 56594.5 52 55494.5 53 55422.5 54 55868.0 55 53260.0 56 48987.5 57 45393.5 58 42346.0 59 37648.5 60 30209.5 61 24847.0 62 20936.0 63 15771.5 64 11234.5 65 8838.0 66 6787.0 67 5234.5 68 4089.5 69 3140.0 70 2466.5 71 1693.0 72 1212.5 73 945.5 74 676.5 75 498.0 76 342.5 77 211.0 78 147.5 79 90.0 80 49.0 81 34.0 82 28.0 83 21.5 84 12.0 85 7.0 86 6.5 87 4.5 88 4.0 89 2.0 90 2.5 91 3.5 92 2.0 93 1.0 94 2.0 95 2.5 96 1.0 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4991158272483382 2 0.05233363526607359 3 0.06086024880411156 4 0.06889256590516181 5 0.08403039428791037 6 0.1120817170869628 7 0.07426803965740314 8 0.054063672795530565 9 0.05227184821145014 10-11 0.07868581406298078 12-13 0.112174397668898 14-15 0.05937735949314843 16-17 0.06447479149958418 18-19 0.06299190218862105 20-21 0.07893296228147463 22-23 0.06812022772236852 24-25 0.09156841495197292 26-27 0.06991205230644897 28-29 0.07068439048924226 30-31 0.07420625260277967 32-33 0.07408267849353274 34-35 0.07655416067847129 36-37 0.07154940925397074 38-39 0.043034683545242335 40-41 0.04695816151383227 42-43 0.04485740165663451 44-45 0.022490487882940717 46-47 0.02953421211001556 48-49 0.02965778621926249 50-51 0.027340771670882606 52-53 0.027248091088947407 54-55 0.026105030578413332 56-57 0.025919669414542944 58-59 0.023355506647669208 60-61 0.03067727262054963 62-63 0.020914917990042397 64-65 0.023509974284227866 66-67 0.017887352313492685 68-69 0.01983364453413179 70-71 0.04501186929319317 72-73 0.040408733723745136 74-75 0.03447717647989264 76-77 0.024220525412397696 78-79 0.01572480540167146 80-81 0.017856458786180956 82-83 0.02230512671907033 84-85 0.01937024162445581 86-87 0.010318438122118407 88-89 0.044239531110399875 90-91 0.05570102974305235 92-93 0.03552755640849152 94-95 0.056658729089716045 96-97 0.046432971549532825 98-99 0.03620721400934962 100-101 0.1698526131599012 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1618462.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.43402376257174 #Duplication Level Percentage of deduplicated Percentage of total 1 80.82677707950285 48.038605895952614 2 11.184927168578861 13.295304542399005 3 3.350286783564022 5.973630729173224 4 1.4374048550996836 3.4172301725772236 5 0.7706260863487236 2.2900704564053855 6 0.4835587101934988 1.7243903923343369 7 0.35982836116959527 1.4970233157740682 8 0.24343477361294996 1.1574646495638674 9 0.19041354339924002 1.0185338756795326 >10 1.0496548666833185 11.35846409703709 >50 0.06684262655004304 2.7069398788584538 >100 0.03155850621839276 3.494940854916183 >500 0.0029161309823909696 1.248813752397077 >1k 0.0014580654911954848 1.2570463288069313 >5k 2.0829507017078354E-4 0.8722198059493043 >10k+ 1.0414753508539177E-4 0.6493212521757292 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10490 0.6481462030001323 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8701 0.537609162278756 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5390 0.3330322244204683 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2140 0.13222429689421192 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1815 0.11214350414158628 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1694 0.10466727053214718 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.17870546234635E-5 0.0 0.0 0.0 0.0 3 6.17870546234635E-5 0.0 0.0 0.0 0.0 4 6.17870546234635E-5 0.0 0.0 0.0 0.0 5 6.17870546234635E-5 0.0 0.0 0.0 0.0 6 6.17870546234635E-5 0.0 0.0 0.0 0.0 7 6.17870546234635E-5 0.0 0.0 6.17870546234635E-5 0.0 8 6.17870546234635E-5 0.0 0.0 1.23574109246927E-4 0.0 9 6.17870546234635E-5 0.0 0.0 1.23574109246927E-4 0.0 10-11 6.17870546234635E-5 0.0 0.0 1.23574109246927E-4 0.0 12-13 6.17870546234635E-5 0.0 0.0 1.23574109246927E-4 0.0 14-15 9.268058193519525E-5 0.0 0.0 1.23574109246927E-4 0.0 16-17 1.23574109246927E-4 0.0 0.0 1.5446763655865877E-4 9.268058193519525E-5 18-19 1.5446763655865877E-4 0.0 0.0 1.8536116387039053E-4 1.8536116387039053E-4 20-21 1.8536116387039053E-4 0.0 0.0 1.8536116387039053E-4 2.47148218493854E-4 22-23 1.8536116387039053E-4 0.0 0.0 4.634029096759763E-4 2.47148218493854E-4 24-25 1.8536116387039053E-4 0.0 0.0 8.032317101050256E-4 4.94296436987708E-4 26-27 1.8536116387039053E-4 0.0 0.0 0.00176093105676871 5.560834916111716E-4 28-29 1.8536116387039053E-4 0.0 0.0 0.004386880878265909 5.560834916111716E-4 30-31 1.8536116387039053E-4 0.0 0.0 0.0123574109246927 5.560834916111716E-4 32-33 1.8536116387039053E-4 0.0 0.0 0.029843147383132873 6.178705462346351E-4 34-35 1.8536116387039053E-4 0.0 0.0 0.05551566857918197 6.178705462346351E-4 36-37 2.47148218493854E-4 0.0 0.0 0.10024949612656955 6.178705462346351E-4 38-39 2.47148218493854E-4 0.0 0.0 0.16398284297067217 6.178705462346351E-4 40-41 2.47148218493854E-4 0.0 0.0 0.22700563868660495 6.178705462346351E-4 42-43 2.47148218493854E-4 0.0 0.0 0.28993575382060255 6.178705462346351E-4 44-45 2.47148218493854E-4 0.0 0.0 0.36914675784788276 6.178705462346351E-4 46-47 2.47148218493854E-4 0.0 0.0 0.45978836698050374 6.178705462346351E-4 48-49 2.47148218493854E-4 0.0 0.0 0.5542298799724676 6.178705462346351E-4 50-51 2.47148218493854E-4 0.0 0.0 0.6560549459919356 6.178705462346351E-4 52-53 2.47148218493854E-4 0.0 0.0 0.7549142333894772 6.178705462346351E-4 54-55 2.47148218493854E-4 0.0 0.0 0.8616822637788222 6.178705462346351E-4 56-57 2.47148218493854E-4 0.0 0.0 0.9802207280739369 6.487640735463668E-4 58-59 2.47148218493854E-4 0.0 0.0 1.1087995887453643 6.796576008580986E-4 60-61 2.47148218493854E-4 0.0 0.0 1.2448855765535427 6.796576008580986E-4 62-63 2.47148218493854E-4 0.0 0.0 1.3788399109772116 7.414446554815621E-4 64-65 2.47148218493854E-4 0.0 0.0 1.517551848606887 7.414446554815621E-4 66-67 2.47148218493854E-4 0.0 0.0 1.6781981906278924 7.414446554815621E-4 68-69 2.47148218493854E-4 0.0 0.0 1.8540441480862695 7.414446554815621E-4 70-71 2.47148218493854E-4 0.0 0.0 2.0323615877295853 7.414446554815621E-4 72-73 3.0893527311731754E-4 0.0 0.0 2.2156837787974015 7.414446554815621E-4 74-75 3.7072232774078105E-4 0.0 0.0 2.403114808997678 8.032317101050256E-4 76-77 3.7072232774078105E-4 0.0 0.0 2.5969716928787947 9.268058193519526E-4 78-79 3.7072232774078105E-4 0.0 0.0 2.8200229600694984 9.88592873975416E-4 80-81 3.7072232774078105E-4 0.0 0.0 3.0503033126511467 9.88592873975416E-4 82-83 3.7072232774078105E-4 0.0 0.0 3.2891411722981445 0.001112166983222343 84-85 4.016158550525128E-4 0.0 0.0 3.529369240674171 0.001112166983222343 86-87 4.3250938236424457E-4 0.0 0.0 3.7704623278149256 0.001112166983222343 88-89 4.3250938236424457E-4 0.0 0.0 4.032161397672605 0.001112166983222343 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4335 0.0 50.563084 1 GTATCAA 7280 0.0 40.407253 1 ATCAACG 9225 0.0 31.989498 3 TATCAAC 9220 0.0 31.903767 2 TCAACGC 9325 0.0 31.799332 4 CAACGCA 9465 0.0 31.328976 5 AACGCAG 9860 0.0 30.1703 6 ACGCAGA 11075 0.0 26.616144 7 CGCAGAG 11165 0.0 26.359013 8 TAAGGTA 575 0.0 25.619822 4 GTACATG 6470 0.0 25.09485 1 TACATGG 6385 0.0 24.56039 2 GTCCTAC 380 0.0 23.877012 1 TGGTATC 1885 0.0 23.697292 2 GCAGAGT 12260 0.0 23.616962 9 GAGTACT 7660 0.0 22.837526 12-13 ACATGGG 6630 0.0 22.075949 3 GTACTTT 8165 0.0 21.441542 14-15 CAGAGTA 11690 0.0 21.331173 10-11 GTGGTAT 2380 0.0 20.265362 1 >>END_MODULE