FastQCFastQC Report
Thu 26 May 2016
SRR937973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937973_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618462
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102310.6321433558526551No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87150.5384741810434845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56140.34687252465612417No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21000.12975281470927336No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17820.11010453133901198No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17700.10936308668353042No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA16530.1021340012925852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47350.048.5644571
GTATCAA75350.037.201631
TATCAAC96400.028.831822
ATCAACG96550.028.582243
TCAACGC97350.028.3464834
CAACGCA98400.028.0440035
AACGCAG101800.027.2006826
GTACATG68750.024.9475381
TACATGG67550.024.898382
TGGTATC22500.024.4945052
CGCAGAG113450.024.3237578
ACGCAGA113600.024.291647
ACATGGG67650.024.2227653
GTGGTAT25450.023.1487261
GAGTACT75750.022.35300412-13
GCAGAGT126650.021.4886179
TAAGGTG8000.021.3732975
GTGTAAG6450.021.3614861
GTACTTT80300.020.99770414-15
CAGAGTA118400.020.67930610-11
Produced by FastQC (version 0.11.5)