##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937973_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1618462 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51537879789578 34.0 31.0 34.0 31.0 34.0 2 32.73170454419072 34.0 31.0 34.0 31.0 34.0 3 32.783959092026876 34.0 31.0 34.0 31.0 34.0 4 36.24223738339238 37.0 35.0 37.0 35.0 37.0 5 36.20795854335783 37.0 37.0 37.0 35.0 37.0 6 36.24990824622388 37.0 37.0 37.0 35.0 37.0 7 36.21395126978576 37.0 37.0 37.0 35.0 37.0 8 36.206592431580106 37.0 37.0 37.0 35.0 37.0 9 37.9227198414297 39.0 38.0 39.0 35.0 39.0 10-11 37.90861849088826 39.0 38.0 39.0 35.0 39.0 12-13 37.85668863402415 39.0 38.0 39.0 35.0 39.0 14-15 39.173085311857804 40.5 39.0 41.0 36.0 41.0 16-17 39.127998371293245 40.0 38.5 41.0 36.0 41.0 18-19 39.15936673211975 40.0 39.0 41.0 36.0 41.0 20-21 39.092184431886565 40.0 39.0 41.0 36.0 41.0 22-23 38.94797622681286 40.0 38.0 41.0 35.0 41.0 24-25 38.90359860163538 40.0 38.0 41.0 35.0 41.0 26-27 38.85849868578934 40.0 38.0 41.0 35.0 41.0 28-29 38.79932151635318 40.0 38.0 41.0 35.0 41.0 30-31 38.590839636642684 40.0 38.0 41.0 34.5 41.0 32-33 38.40041317003427 40.0 38.0 41.0 34.0 41.0 34-35 38.1952291743643 40.0 38.0 41.0 34.0 41.0 36-37 38.0572657869014 40.0 38.0 41.0 33.5 41.0 38-39 38.035298635371106 40.0 38.0 41.0 33.0 41.0 40-41 37.96744501878945 40.0 38.0 41.0 33.0 41.0 42-43 37.80784164225048 40.0 37.0 41.0 33.0 41.0 44-45 37.5922737141805 40.0 37.0 41.0 33.0 41.0 46-47 37.25318821201857 40.0 37.0 41.0 32.5 41.0 48-49 36.892383015480135 40.0 36.0 41.0 31.0 41.0 50-51 36.59078248361716 40.0 36.0 41.0 31.0 41.0 52-53 36.180563708014155 39.0 35.0 41.0 30.0 41.0 54-55 35.95751583911145 39.0 35.0 41.0 29.0 41.0 56-57 35.709850154035124 39.0 35.0 40.5 29.0 41.0 58-59 35.369364248280164 38.0 35.0 40.0 28.0 41.0 60-61 35.012058979450856 38.0 34.5 40.0 27.5 41.0 62-63 34.65590047835538 37.0 34.0 40.0 26.5 41.0 64-65 34.262091726589816 37.0 34.0 40.0 26.0 41.0 66-67 33.84413103304248 36.0 33.5 39.0 26.0 41.0 68-69 33.393321869775136 36.0 33.0 39.0 25.0 40.5 70-71 32.87296241740616 35.0 32.5 38.5 23.5 40.0 72-73 32.45859680363209 35.0 32.0 37.5 22.5 40.0 74-75 32.01607235758393 35.0 32.0 37.0 21.5 39.0 76-77 30.725005900663717 34.0 30.0 35.5 20.5 38.5 78-79 31.445600823497863 35.0 31.5 36.0 21.0 38.5 80-81 31.28287442028296 35.0 32.0 36.0 20.0 37.0 82-83 30.918615327391066 35.0 31.0 35.0 18.5 37.0 84-85 30.535849775898352 34.0 31.0 35.0 17.5 36.5 86-87 30.107897188812586 34.0 31.0 35.0 11.0 36.0 88-89 29.64811592734337 34.0 30.0 35.0 7.0 36.0 90-91 29.29972591262569 34.0 30.0 35.0 2.0 35.0 92-93 28.88518451468122 34.0 29.0 35.0 2.0 35.0 94-95 28.47034252271601 34.0 29.0 35.0 2.0 35.0 96-97 27.893562221417618 34.0 29.0 35.0 2.0 35.0 98-99 27.204159257369035 33.5 27.0 35.0 2.0 35.0 100-101 24.756447170214685 31.0 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13383896198463674 1101 2 0.19923654329541307 1101 3 0.202107480118336 1101 4 0.0760271275454798 1101 5 0.07917791448755906 1101 6 0.028506806774835525 1101 7 0.10647439930452407 1101 8 0.08825342902240152 1101 9 0.07676897930532078 1101 10-11 0.07400134285823867 1101 12-13 0.14181068751169335 1101 14-15 0.2677979571064597 1101 16-17 0.20661875507573058 1101 18-19 0.23519899448838544 1101 20-21 0.15618206444029425 1101 22-23 0.26389275443953863 1101 24-25 0.13472890998484388 1101 26-27 0.1314385364292221 1101 28-29 0.14197146628812618 1101 30-31 0.14808380770424634 1101 32-33 0.16306855678254095 1101 34-35 4.099707615878856E-4 1101 36-37 0.17684488009598454 1101 38-39 0.08741145082370139 1101 40-41 0.11388417982225718 1101 42-43 0.11446733193341174 1101 44-45 0.05832441562557733 1101 46-47 0.11462379046961502 1101 48-49 -0.035695174283326025 1101 50-51 -0.009104125279321806 1101 52-53 0.02803555018534354 1101 54-55 0.059769100557545585 1101 56-57 0.12789113435749044 1101 58-59 -0.0053629855285706185 1101 60-61 0.11262033122950754 1101 62-63 -0.007126538335768373 1101 64-65 0.026060382563883877 1101 66-67 -0.02057311297681963 1101 68-69 -0.0047114583001217625 1101 70-71 -0.10987405036802045 1101 72-73 -0.07472551497706803 1101 74-75 -0.08562237471609535 1101 76-77 -0.09837955675630994 1101 78-79 -0.043531720431570164 1101 80-81 0.19398571972217837 1101 82-83 0.23938462306298547 1101 84-85 0.28709667093102453 1101 86-87 0.2934919967381475 1101 88-89 0.1143649141915617 1101 90-91 0.27732002022997904 1101 92-93 0.4330639364046256 1101 94-95 0.28870409675278097 1101 96-97 0.42437531945343565 1101 98-99 0.6386717319297013 1101 100-101 0.9430795568601944 1102 1 0.10257902747962788 1102 2 0.15204436806624955 1102 3 0.11415143072920131 1102 4 0.057374185104407616 1102 5 0.08026100097624322 1102 6 0.007693462687583974 1102 7 -0.003152036855176732 1102 8 0.05754032814866861 1102 9 0.08489020419342097 1102 10-11 0.10057760162165152 1102 12-13 0.2138686432196124 1102 14-15 0.24161823689097872 1102 16-17 0.15943090834497298 1102 18-19 0.23214860547660976 1102 20-21 0.12188448594658041 1102 22-23 0.08977265399913392 1102 24-25 0.059198777143230075 1102 26-27 -0.024202242450400036 1102 28-29 0.058679133453530596 1102 30-31 0.13177546856351086 1102 32-33 0.05505424580493923 1102 34-35 0.10980940122738758 1102 36-37 0.11324456979075137 1102 38-39 0.06723199128001056 1102 40-41 -0.030156223343702493 1102 42-43 -0.07316080965465233 1102 44-45 0.13422776121818458 1102 46-47 0.10424645602626015 1102 48-49 0.06446267162501584 1102 50-51 0.13640003176244875 1102 52-53 0.07429276975997112 1102 54-55 0.08365135001082535 1102 56-57 0.15468862748358703 1102 58-59 0.09336020363210196 1102 60-61 0.10198641301985845 1102 62-63 0.13518054169287552 1102 64-65 0.17537313725393489 1102 66-67 0.018427138557036926 1102 68-69 0.4065430222969013 1102 70-71 0.4830564055719506 1102 72-73 0.38754296446472836 1102 74-75 0.26474552360074455 1102 76-77 0.384676055310198 1102 78-79 0.5030027635128249 1102 80-81 0.4325641350396765 1102 82-83 0.5081042965210649 1102 84-85 0.5106542349098397 1102 86-87 0.4106603604166601 1102 88-89 0.16682751716773936 1102 90-91 0.09542713902273192 1102 92-93 0.08644836525686017 1102 94-95 0.1366403408570065 1102 96-97 0.1757321834659642 1102 98-99 0.0020888505637444155 1102 100-101 0.2555341376860021 1103 1 0.03794924722699733 1103 2 0.07634727323632973 1103 3 0.04351526869593414 1103 4 0.01849583982551195 1103 5 -0.01023190699773835 1103 6 -0.012113230071619796 1103 7 0.009580936373325244 1103 8 -0.005527144003451667 1103 9 0.1336776890442195 1103 10-11 0.020993274755838343 1103 12-13 0.03592796087424688 1103 14-15 0.12595182280749384 1103 16-17 -0.048981270335623606 1103 18-19 -0.0031340212031238934 1103 20-21 0.06674047877228872 1103 22-23 0.10108861239011446 1103 24-25 -0.014130755855951804 1103 26-27 -0.03917324172972059 1103 28-29 0.026413369597953817 1103 30-31 0.07536924065005479 1103 32-33 0.12315368479219302 1103 34-35 0.03618279332686569 1103 36-37 0.04047489089580836 1103 38-39 0.14892596705519878 1103 40-41 0.1612763404219706 1103 42-43 0.1421784049275061 1103 44-45 0.3419340973484708 1103 46-47 0.34439953533969714 1103 48-49 0.34981400556547015 1103 50-51 0.24169315570664196 1103 52-53 0.3198149797006238 1103 54-55 0.4158710000556667 1103 56-57 0.45919155999753514 1103 58-59 0.5732724251823171 1103 60-61 0.4148438240190444 1103 62-63 0.39093584651907065 1103 64-65 0.16004805806099398 1103 66-67 0.2636337353809779 1103 68-69 0.45206384187140713 1103 70-71 0.34560527114903294 1103 72-73 0.05684808263426788 1103 74-75 0.11451738768781894 1103 76-77 0.25204925971024394 1103 78-79 0.16235100620587062 1103 80-81 0.30268910578405794 1103 82-83 0.37447133928177223 1103 84-85 0.35054244947114555 1103 86-87 0.45432040662316453 1103 88-89 0.2545338996435653 1103 90-91 0.13028361847659653 1103 92-93 0.13899748573440363 1103 94-95 0.015112838255863181 1103 96-97 0.14387217479935188 1103 98-99 0.2563770873709075 1103 100-101 0.5062203992463168 1104 1 0.10415973924393995 1104 2 0.08551638369576153 1104 3 0.07183719766302232 1104 4 0.057692103537902995 1104 5 0.07933255482478785 1104 6 0.0028536429597707524 1104 7 0.027495566546015482 1104 8 0.054807384073008336 1104 9 0.1367166811154732 1104 10-11 0.01698436848886331 1104 12-13 0.12011933700110689 1104 14-15 0.19986080510357596 1104 16-17 0.0786096930618001 1104 18-19 0.1646730820878517 1104 20-21 0.0835635481760022 1104 22-23 0.11968207388679275 1104 24-25 0.0710283622063912 1104 26-27 0.10189659643689453 1104 28-29 0.03279764243383454 1104 30-31 -0.004051851996841549 1104 32-33 0.04402367501702287 1104 34-35 0.15313511221467024 1104 36-37 0.08266778760483362 1104 38-39 0.05840353846825508 1104 40-41 0.004277317939077818 1104 42-43 0.03946090737130703 1104 44-45 -0.08379523902603125 1104 46-47 -0.08608922321357682 1104 48-49 -0.01732295544245943 1104 50-51 0.06691309705561821 1104 52-53 0.0733199215926632 1104 54-55 0.0026549687751185047 1104 56-57 0.07155606526526981 1104 58-59 0.02335931559178306 1104 60-61 -0.01116790618623753 1104 62-63 0.012635640154357475 1104 64-65 -0.08737889968853807 1104 66-67 0.027122385321241893 1104 68-69 0.018771781482570304 1104 70-71 -0.10614013995121496 1104 72-73 -0.1741696212866657 1104 74-75 -0.042746650543961096 1104 76-77 -0.14598375864535384 1104 78-79 -0.15448075223879343 1104 80-81 -0.18315861877334427 1104 82-83 0.07014745528047328 1104 84-85 0.165547337056676 1104 86-87 0.021900508719394196 1104 88-89 -0.14165704203124818 1104 90-91 0.03596950964635681 1104 92-93 -0.015770625485320267 1104 94-95 0.084000641014633 1104 96-97 0.25825688208728437 1104 98-99 0.18438621086390228 1104 100-101 0.12858055999357632 1105 1 0.018640608055768837 1105 2 0.11681819103392144 1105 3 0.006320081344590278 1105 4 0.02925342609839987 1105 5 0.05877967456849831 1105 6 -0.07407408366270118 1105 7 -0.014893665990101113 1105 8 0.009426472948916853 1105 9 -0.001647244656723501 1105 10-11 0.0976684167651456 1105 12-13 0.08133813170603332 1105 14-15 0.16625197191464025 1105 16-17 0.10452716399730377 1105 18-19 0.08715298376220915 1105 20-21 0.03745385866743334 1105 22-23 0.1276595476431197 1105 24-25 0.06938060173410321 1105 26-27 0.0414144381084256 1105 28-29 0.05080574221018708 1105 30-31 0.23395846413621513 1105 32-33 0.18054016672584083 1105 34-35 0.12910094002390338 1105 36-37 0.22982561235146193 1105 38-39 0.2600158372331407 1105 40-41 0.33580741001861725 1105 42-43 0.23799730599313307 1105 44-45 0.40713724268903206 1105 46-47 0.41403025856192954 1105 48-49 0.5474911728241523 1105 50-51 0.6540320348162894 1105 52-53 0.6578122751620015 1105 54-55 0.588477136371985 1105 56-57 0.7030500911764932 1105 58-59 0.6867219316986564 1105 60-61 0.6613204579410237 1105 62-63 0.635490912061023 1105 64-65 0.5246051425431304 1105 66-67 0.5703772058970884 1105 68-69 0.4100284557385834 1105 70-71 0.49763693486089977 1105 72-73 0.34537945086582056 1105 74-75 0.3716876747769078 1105 76-77 0.4610596215001195 1105 78-79 0.5795868667896364 1105 80-81 0.5449951560851112 1105 82-83 0.6450528571462399 1105 84-85 0.6927793090096905 1105 86-87 0.6390645795622127 1105 88-89 0.6941953486116965 1105 90-91 0.7032651514831159 1105 92-93 0.6212556282673738 1105 94-95 0.7446331086256031 1105 96-97 0.9069901334825801 1105 98-99 1.032450122532012 1105 100-101 0.903172851101953 1106 1 -0.12027342897320636 1106 2 -0.03352674787364407 1106 3 -0.03038099733516475 1106 4 -0.050194812011206125 1106 5 -0.0537494797287934 1106 6 -0.04984258353130855 1106 7 -0.017521549156128913 1106 8 -0.09745390222234818 1106 9 -0.11708461565066841 1106 10-11 -0.03561399645818142 1106 12-13 -0.13679742027809993 1106 14-15 -0.29870107319371897 1106 16-17 -0.1712167335823196 1106 18-19 -0.10620404730686062 1106 20-21 -0.07749400085545233 1106 22-23 -0.08182289973488821 1106 24-25 -0.06575751029169652 1106 26-27 -0.027821900603100858 1106 28-29 0.044648151877680675 1106 30-31 0.044916775927923425 1106 32-33 -0.01833990276880826 1106 34-35 0.1398013761589354 1106 36-37 0.02269597817307556 1106 38-39 0.04040063886694867 1106 40-41 0.12180862960594396 1106 42-43 0.06316353714859702 1106 44-45 0.21391608955518393 1106 46-47 0.17326118554966286 1106 48-49 0.270343268229567 1106 50-51 0.18005996991615092 1106 52-53 0.05113756888837884 1106 54-55 -0.11694593332306624 1106 56-57 -0.01914990710798037 1106 58-59 -0.04742518765012704 1106 60-61 0.025722271414345244 1106 62-63 0.06849041845566717 1106 64-65 0.008474267723158846 1106 66-67 -0.02479739237599432 1106 68-69 -0.035255039829500845 1106 70-71 -0.03914485180333571 1106 72-73 -0.05996801845149946 1106 74-75 0.04587402522422934 1106 76-77 0.061717099153366206 1106 78-79 0.09265025335905008 1106 80-81 -0.17434906619793722 1106 82-83 -0.3412987208703093 1106 84-85 -0.01027925067640112 1106 86-87 0.08237813748987932 1106 88-89 0.03101784017244924 1106 90-91 -0.15943945495434164 1106 92-93 -0.037034368387338645 1106 94-95 -0.14943337291956027 1106 96-97 -0.07327306032341241 1106 98-99 0.1258811164254965 1106 100-101 0.246348269711266 1107 1 0.08375551898746636 1107 2 0.06927597303526056 1107 3 0.1348561585101038 1107 4 0.11254179952032217 1107 5 0.005512540807778521 1107 6 0.08712617909145592 1107 7 0.006593931859598001 1107 8 0.008439494390664493 1107 9 0.0207084982124357 1107 10-11 0.07190244474897156 1107 12-13 0.05507757010019532 1107 14-15 0.17142673240454087 1107 16-17 0.1067844510789584 1107 18-19 0.09430958692750835 1107 20-21 0.0627386282055511 1107 22-23 0.11428912406390168 1107 24-25 0.17794897668179743 1107 26-27 0.11951503069900582 1107 28-29 0.08123739770442029 1107 30-31 0.1272831734800164 1107 32-33 0.0849448198657754 1107 34-35 0.0774239383848041 1107 36-37 0.03557385469706276 1107 38-39 0.12290375630016115 1107 40-41 0.23201603329906817 1107 42-43 0.05535657535759242 1107 44-45 0.007777696779854182 1107 46-47 0.13973219250441815 1107 48-49 0.19688858775455742 1107 50-51 0.2262978639754749 1107 52-53 0.15042900802457382 1107 54-55 0.17394320774619842 1107 56-57 0.18433692636427423 1107 58-59 0.24392927751855353 1107 60-61 0.12503973497830856 1107 62-63 0.33182170648890263 1107 64-65 0.43632007555438435 1107 66-67 0.37283753695118804 1107 68-69 0.3632848798099957 1107 70-71 0.36446543275728516 1107 72-73 0.40621452055032137 1107 74-75 0.37682355762757425 1107 76-77 0.2842155101971038 1107 78-79 0.40202351916082435 1107 80-81 0.4779233428503531 1107 82-83 0.5286152445707195 1107 84-85 0.6483982832048376 1107 86-87 0.6007255423412765 1107 88-89 0.6619830238865809 1107 90-91 0.7332807062065108 1107 92-93 0.8780268476645254 1107 94-95 0.7584091800254882 1107 96-97 0.9962873027869961 1107 98-99 1.0961601202837095 1107 100-101 1.0821136041471568 1108 1 0.06817189500377197 1108 2 0.0974618715305624 1108 3 -0.014671041450590394 1108 4 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 6.0 4 14.0 5 27.0 6 62.0 7 140.0 8 369.0 9 681.0 10 1194.0 11 1846.0 12 2787.0 13 4075.0 14 5583.0 15 7820.0 16 10118.0 17 14164.0 18 14369.0 19 10057.0 20 8699.0 21 8450.0 22 8767.0 23 9404.0 24 10615.0 25 12304.0 26 14471.0 27 17541.0 28 21655.0 29 26076.0 30 31921.0 31 39572.0 32 49544.0 33 65087.0 34 91928.0 35 142909.0 36 231668.0 37 376889.0 38 337962.0 39 39676.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.42122335896672 18.83306497155942 14.991454850345576 22.754256819128283 2 15.92503307673246 19.956841121067946 39.86282348302782 24.255302319171776 3 19.48857408032191 23.977871124573483 29.931880731960813 26.601674063143793 4 13.120604623401725 16.828445771355767 36.082651307228716 33.96829829801379 5 13.667111121546258 37.197104411472125 34.13092182578275 15.004862641198866 6 32.61763328394488 35.76080253969509 16.798911559245752 14.822652617114274 7 28.672097336854375 32.99317500194629 20.089566514382174 18.245161146817164 8 26.81061456103842 34.82423989283613 19.052973945538216 19.312171600587227 9 26.404890098822705 16.816480418967732 19.90967923709911 36.868950245110454 10-11 25.36964105428487 26.601149733512436 26.431204439770596 21.5980047724321 12-13 26.280783855289773 23.900746511193958 27.268542604027772 22.54992702948849 14-15 22.82274776917839 25.287093549307922 25.88108339893059 26.009075282583094 16-17 22.06961811798638 28.538089959702383 26.798409350134513 22.593882572176728 18-19 22.033912442800634 26.606834142537792 29.5661869107832 21.79306650387837 20-21 22.533553460013273 25.675486974671013 30.080409672886976 21.710549892428737 22-23 22.305590121980003 25.61150647960842 30.046334112262134 22.036569286149444 24-25 22.137247038861677 25.922202497434167 30.094427702747375 21.84612276095678 26-27 21.652335907998044 26.26355762281311 29.938240800623184 22.14586566856566 28-29 21.750934452256047 26.62395738174804 29.80450878812996 21.820599377865953 30-31 21.856089299594306 26.15100014705319 29.764523356124517 22.228387197227985 32-33 21.56351485613449 26.234602608906265 29.715296554246763 22.48658598071248 34-35 22.329346207575053 26.323465477749046 29.528481772425767 21.818706542250133 36-37 22.378558936140042 25.92945476991748 29.55832844427102 22.13365784967145 38-39 21.842293583424382 26.26095054703297 29.532828157984508 22.363927711558144 40-41 22.235298806824307 26.12136175961049 29.6645222647134 21.9788171688518 42-43 22.018107409863052 26.176187619064684 29.36253315891625 22.443171812156013 44-45 21.918919144363812 26.41546099084818 29.56006825168845 22.105551613099554 46-47 22.405561576360768 26.65363783641507 28.84349462637986 22.097305960844306 48-49 22.149516022001134 26.770168221434922 28.70623468453384 22.374081072030112 50-51 22.310934702204932 26.921268463516597 28.649112552534444 22.118684281744024 52-53 22.585392798842356 26.744989996675855 28.517135403858724 22.15248180062306 54-55 22.166074952640223 26.999151045869475 28.523931979867307 22.310842021623 56-57 22.171110597592033 27.14796516693009 28.325193918670937 22.355730316806945 58-59 22.19567095180486 26.74789398824316 28.404404922698216 22.65203013725376 60-61 21.95803793972302 26.984105898068663 28.652201905265617 22.405654256942704 62-63 22.018743720890573 26.967299819211078 28.795949487847107 22.218006972051242 64-65 21.848427704820995 26.98660827378091 28.625509897668277 22.53945412372981 66-67 21.693743813571157 27.163782652913692 28.472895872748328 22.669577660766826 68-69 22.188242701311157 26.822498074556744 28.55986112711571 22.42939809701639 70-71 22.089829727234868 26.910702877175986 28.2592671313877 22.740200264201444 72-73 22.007900092804157 27.056118710232308 28.145918779680954 22.79006241728258 74-75 21.892080258912475 27.443029246284432 28.060034773754346 22.60485572104875 76-77 22.07876984445727 27.011261308575673 28.20294823109841 22.707020615868643 78-79 22.003729466617074 27.24524888443473 28.26445724397607 22.48656440497213 80-81 22.213774558809536 27.297767880864672 27.930745361954745 22.557712198371046 82-83 22.106024764877013 27.321996847005632 28.045461693095575 22.526516695021783 84-85 21.98118302159127 27.462506920278397 27.95835722477064 22.597952833359695 86-87 22.144617417891677 27.344108414405948 27.835255380666702 22.676018787035673 88-89 22.074884079726335 27.461882781888846 27.622590960612904 22.840642177771915 90-91 21.973563115767256 27.48294132966647 27.69817699990454 22.845318554661738 92-93 22.10312402105954 27.148324424653325 27.87407651352305 22.874475040764086 94-95 21.912593561047462 26.896090238757537 28.071681633550867 23.119634566644134 96-97 22.100302632993547 26.74486642256661 27.98755855868102 23.167272385758825 98-99 21.809378286298966 27.032516055366145 27.705871376652645 23.452234281682237 100-101 21.892296513603657 26.70417964709706 28.056296656949627 23.347227182349663 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1714.0 1 1154.5 2 952.0 3 1254.5 4 904.0 5 733.5 6 1467.0 7 3120.5 8 4910.0 9 5468.5 10 4106.0 11 2580.5 12 2239.5 13 2266.0 14 2365.5 15 2412.5 16 2457.5 17 2588.0 18 2683.0 19 2834.0 20 3137.5 21 3658.0 22 4338.5 23 5311.0 24 6472.0 25 7807.5 26 9758.5 27 12011.5 28 13802.0 29 15629.5 30 17921.5 31 21021.5 32 25619.5 33 27892.5 34 28449.5 35 30473.5 36 33764.0 37 37235.0 38 40058.5 39 42271.0 40 44275.5 41 47128.5 42 48891.5 43 50524.0 44 52989.0 45 56063.0 46 57187.5 47 58131.0 48 59922.0 49 59694.5 50 58553.5 51 57066.0 52 56082.0 53 56335.0 54 57135.5 55 54042.5 56 49287.0 57 45512.5 58 42593.5 59 37960.0 60 30209.5 61 24559.5 62 20724.0 63 15467.5 64 10848.5 65 8360.0 66 6205.0 67 4825.0 68 3822.0 69 2913.5 70 2201.5 71 1403.0 72 975.5 73 786.0 74 581.5 75 435.5 76 308.5 77 199.0 78 119.0 79 69.0 80 33.5 81 20.0 82 14.5 83 8.5 84 5.5 85 1.5 86 1.0 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015755698928983194 3 0.007661594773309476 4 0.0 5 0.0 6 0.0 7 0.0 8 3.7072232774078105E-4 9 4.942964369877081E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.4714821849385407E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005004751424500544 26-27 0.019926325116066984 28-29 3.089352731173176E-5 30-31 0.0 32-33 3.7072232774078105E-4 34-35 0.018258074641233468 36-37 0.0033056074223552978 38-39 0.017176801185322856 40-41 0.013438684380603314 42-43 0.007352659500192158 44-45 0.002502375712250272 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.162546911821223E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.089352731173176E-5 84-85 0.0018536116387039055 86-87 3.398288004290493E-4 88-89 3.089352731173176E-5 90-91 2.162546911821223E-4 92-93 1.8536116387039053E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1618462.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.728002765849105 #Duplication Level Percentage of deduplicated Percentage of total 1 79.34544848360981 45.80454269519365 2 12.16236812125347 14.042184410859907 3 3.5426848525997854 6.135363629082365 4 1.5217766826880723 3.513965141888869 5 0.8382061214330678 2.4193982648219894 6 0.5190092763092676 1.7976821362969648 7 0.3657199345391967 1.4778596974823432 8 0.27249993354605045 1.2584701533952076 9 0.19014485817001345 0.9879014608515471 >10 1.1313799719261433 11.90271119013404 >50 0.07125548019148964 2.835590134141783 >100 0.03436747991528139 3.666184733908775 >500 0.0032117523864925654 1.2969293143014036 >1k 0.0016058761932462827 1.3433431296476201 >5k 2.14116825766171E-4 0.8855706525911275 >10k+ 1.070584128830855E-4 0.6323032554023189 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10231 0.6321433558526551 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8715 0.5384741810434845 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5614 0.34687252465612417 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2100 0.12975281470927336 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1782 0.11010453133901198 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1770 0.10936308668353042 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1653 0.1021340012925852 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 3.089352731173175E-5 0.0 0.0 0.0 6.17870546234635E-5 14-15 6.17870546234635E-5 0.0 0.0 0.0 1.5446763655865877E-4 16-17 6.17870546234635E-5 0.0 0.0 3.089352731173175E-5 2.47148218493854E-4 18-19 6.17870546234635E-5 0.0 0.0 6.17870546234635E-5 2.780417458055858E-4 20-21 6.17870546234635E-5 0.0 0.0 6.17870546234635E-5 3.398288004290493E-4 22-23 6.17870546234635E-5 0.0 0.0 3.398288004290493E-4 3.7072232774078105E-4 24-25 1.8536116387039053E-4 0.0 0.0 6.796576008580986E-4 3.7072232774078105E-4 26-27 1.8536116387039053E-4 0.0 0.0 0.001637356947521783 3.7072232774078105E-4 28-29 2.1625469118212226E-4 0.0 0.0 0.0042324132417072505 3.7072232774078105E-4 30-31 2.47148218493854E-4 0.0 0.0 0.012295623870069237 3.7072232774078105E-4 32-33 2.47148218493854E-4 0.0 0.0 0.02965778621926248 3.7072232774078105E-4 34-35 2.47148218493854E-4 0.0 0.0 0.05520673330606465 3.7072232774078105E-4 36-37 2.47148218493854E-4 0.0 0.0 0.09987877379882876 3.7072232774078105E-4 38-39 2.47148218493854E-4 0.0 0.0 0.1640137364979839 3.7072232774078105E-4 40-41 2.47148218493854E-4 0.0 0.0 0.22682027752273454 3.7072232774078105E-4 42-43 2.780417458055858E-4 0.0 0.0 0.2901211149844729 3.7072232774078105E-4 44-45 3.7072232774078105E-4 0.0 0.0 0.36998088308529953 4.016158550525128E-4 46-47 3.7072232774078105E-4 0.0 0.0 0.4612403627641551 4.3250938236424457E-4 48-49 3.7072232774078105E-4 0.0 0.0 0.5556509822288074 4.3250938236424457E-4 50-51 3.7072232774078105E-4 0.0 0.0 0.6575378353028987 4.3250938236424457E-4 52-53 3.7072232774078105E-4 0.0 0.0 0.7567060579735576 4.3250938236424457E-4 54-55 3.7072232774078105E-4 0.0 0.0 0.8636594495267729 4.3250938236424457E-4 56-57 3.7072232774078105E-4 0.0 0.0 0.9825068490950051 4.3250938236424457E-4 58-59 3.7072232774078105E-4 0.0 0.0 1.1112092838756795 4.3250938236424457E-4 60-61 3.7072232774078105E-4 0.0 0.0 1.2480676098666512 4.3250938236424457E-4 62-63 3.7072232774078105E-4 0.0 0.0 1.3818365831264496 4.3250938236424457E-4 64-65 3.7072232774078105E-4 0.0 0.0 1.5206720948653722 4.94296436987708E-4 66-67 4.94296436987708E-4 0.0 0.0 1.6817509462687417 4.94296436987708E-4 68-69 4.94296436987708E-4 0.0 0.0 1.8587399642376528 4.94296436987708E-4 70-71 4.94296436987708E-4 0.0 0.0 2.037767955009138 4.94296436987708E-4 72-73 4.94296436987708E-4 0.0 0.0 2.2215535489866305 6.178705462346351E-4 74-75 4.94296436987708E-4 0.0 0.0 2.4098187044243238 6.796576008580986E-4 76-77 4.94296436987708E-4 0.0 0.0 2.605127584089092 6.796576008580986E-4 78-79 4.94296436987708E-4 0.0 0.0 2.829105657099147 6.796576008580986E-4 80-81 4.94296436987708E-4 0.0 0.0 3.062166427138852 7.105511281698303E-4 82-83 4.94296436987708E-4 0.0 0.0 3.3037229171892823 7.414446554815621E-4 84-85 5.560834916111716E-4 0.0 0.0 3.5475655282607805 7.414446554815621E-4 86-87 6.178705462346351E-4 0.0 0.0 3.79230405162432 8.341252374167574E-4 88-89 6.178705462346351E-4 0.0 0.0 4.05758677065016 9.268058193519527E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4735 0.0 48.564457 1 GTATCAA 7535 0.0 37.20163 1 TATCAAC 9640 0.0 28.83182 2 ATCAACG 9655 0.0 28.58224 3 TCAACGC 9735 0.0 28.346483 4 CAACGCA 9840 0.0 28.044003 5 AACGCAG 10180 0.0 27.200682 6 GTACATG 6875 0.0 24.947538 1 TACATGG 6755 0.0 24.89838 2 TGGTATC 2250 0.0 24.494505 2 CGCAGAG 11345 0.0 24.323757 8 ACGCAGA 11360 0.0 24.29164 7 ACATGGG 6765 0.0 24.222765 3 GTGGTAT 2545 0.0 23.148726 1 GAGTACT 7575 0.0 22.353004 12-13 GCAGAGT 12665 0.0 21.488617 9 TAAGGTG 800 0.0 21.373297 5 GTGTAAG 645 0.0 21.361486 1 GTACTTT 8030 0.0 20.997704 14-15 CAGAGTA 11840 0.0 20.679306 10-11 >>END_MODULE