FastQCFastQC Report
Thu 26 May 2016
SRR937972_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937972_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651656
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103170.6246458100233947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83660.5065219391931491No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52860.32004243014283845No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21990.1331391040265043No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18350.11110061659328577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47450.047.756841
GTATCAA78800.041.2628631
ATCAACG98900.032.6495133
TATCAAC99350.032.645232
TCAACGC99400.032.4852834
CAACGCA100750.031.8611875
AACGCAG103600.030.9752966
ACGCAGA116150.027.341077
CGCAGAG115900.027.3146958
TGGTATC21850.026.7705272
GTGGTAT24600.024.5772611
GCAGAGT126900.024.460049
GAGTACT77800.023.21859412-13
GTACATG69900.022.6113131
TACATGG69050.022.3832382
GTACTTT81700.021.99253514-15
CAGAGTA121350.021.60155310-11
ACATGGG70300.021.5117153
GTATAGG5350.021.356121
AGAGTAC113600.021.1924110-11
Produced by FastQC (version 0.11.5)