FastQCFastQC Report
Thu 26 May 2016
SRR937972_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937972_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651656
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100850.6105993015494752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86680.524806618327303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55720.337358384554653No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22090.13374455697796636No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17760.10752844417965968No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17700.10716517240878246No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA16740.1013528240747468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43100.053.0241661
GTATCAA73700.040.4853741
TGGTATC19500.031.9146522
TATCAAC95150.031.2050342
ATCAACG95500.031.0334053
TCAACGC96800.030.5148074
CAACGCA98800.029.89715
AACGCAG102700.028.900496
GTGGTAT22700.028.2562121
ACGCAGA116150.025.3085377
CGCAGAG116700.025.067188
GCAGAGT128450.022.5153569
GAGTACT78750.022.25279612-13
GTACTTT82300.021.1486614-15
GTCTATA3650.020.8273091
AGTACTT81050.020.80099512-13
CAGAGTA123200.020.66154710-11
AGAGTAC115600.020.5615110-11
GTACATG68350.020.2978651
GTAAGGT9200.020.1313274
Produced by FastQC (version 0.11.5)