##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937971_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2689989 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.522493214656265 33.0 31.0 34.0 31.0 34.0 2 32.740052840364775 34.0 31.0 34.0 31.0 34.0 3 32.79830437968334 34.0 31.0 34.0 31.0 34.0 4 36.2531817044605 37.0 35.0 37.0 35.0 37.0 5 36.18101709709594 37.0 37.0 37.0 35.0 37.0 6 36.242145599851895 37.0 37.0 37.0 35.0 37.0 7 36.214067418119555 37.0 37.0 37.0 35.0 37.0 8 36.2037543647948 37.0 37.0 37.0 35.0 37.0 9 37.90988104412323 39.0 38.0 39.0 35.0 39.0 10-11 37.87984225957801 39.0 38.0 39.0 35.0 39.0 12-13 37.809306097534225 39.0 38.0 39.0 35.0 39.0 14-15 39.06182608925167 40.0 38.0 41.0 36.0 41.0 16-17 38.9822962101332 40.0 38.0 41.0 36.0 41.0 18-19 39.237088515975344 40.5 39.0 41.0 36.0 41.0 20-21 39.20797259765746 41.0 39.0 41.0 36.0 41.0 22-23 39.11265139002427 40.0 39.0 41.0 35.5 41.0 24-25 38.988638057627746 40.0 38.5 41.0 35.0 41.0 26-27 38.82015651365117 40.0 38.0 41.0 35.0 41.0 28-29 38.69883984655699 40.0 38.0 41.0 35.0 41.0 30-31 38.426813455371004 40.0 38.0 41.0 34.0 41.0 32-33 38.16079619656438 40.0 38.0 41.0 34.0 41.0 34-35 38.028418331822174 40.0 37.5 41.0 33.5 41.0 36-37 37.93557259899576 40.0 37.5 41.0 33.0 41.0 38-39 37.71709475391907 40.0 37.0 41.0 33.0 41.0 40-41 37.48998378803779 40.0 37.0 41.0 33.0 41.0 42-43 37.2104778123628 40.0 36.5 41.0 32.0 41.0 44-45 36.925015678502774 40.0 36.0 41.0 31.0 41.0 46-47 36.52840309012416 39.0 35.5 41.0 30.5 41.0 48-49 36.0583076733771 39.0 35.0 41.0 29.5 41.0 50-51 35.635155571268136 39.0 35.0 40.0 28.0 41.0 52-53 35.09685838864026 38.0 34.5 40.0 26.5 41.0 54-55 34.706349542693296 38.0 34.0 40.0 26.0 41.0 56-57 34.3059614741919 38.0 34.0 40.0 24.5 41.0 58-59 33.79672444757209 37.0 33.0 40.0 23.0 41.0 60-61 33.30387317568957 36.5 33.0 39.5 22.0 41.0 62-63 32.76567692284244 36.0 32.0 39.0 19.5 40.0 64-65 32.214512215477455 35.0 31.0 39.0 18.0 40.0 66-67 31.65654469219019 35.0 31.0 38.0 13.5 40.0 68-69 31.044299065906962 35.0 30.0 37.5 8.0 40.0 70-71 30.42019558444291 34.5 29.5 37.0 6.5 39.0 72-73 29.776612841167754 34.0 29.0 36.0 2.0 39.0 74-75 29.168946415766012 34.0 28.0 36.0 2.0 38.5 76-77 27.861561143930327 32.0 26.5 34.5 2.0 36.5 78-79 28.523013662881148 33.5 28.0 35.0 2.0 37.0 80-81 28.278212104213065 34.0 27.5 35.0 2.0 36.5 82-83 27.749802880234824 33.0 26.5 35.0 2.0 36.0 84-85 27.129263911488117 33.0 25.5 35.0 2.0 36.0 86-87 26.4834928693017 32.5 24.5 35.0 2.0 35.0 88-89 25.68893497334004 32.0 22.0 34.5 2.0 35.0 90-91 25.05728220449972 31.5 19.0 34.0 2.0 35.0 92-93 24.173956101679224 31.0 11.5 34.0 2.0 35.0 94-95 23.306488428019595 31.0 2.0 34.0 2.0 35.0 96-97 22.29405733629394 30.0 2.0 34.0 2.0 35.0 98-99 20.889084304805706 29.5 2.0 34.0 2.0 35.0 100-101 16.94006889991 21.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15989957660436716 1101 2 0.1689872979339242 1101 3 0.10405060823107704 1101 4 0.07825773466115038 1101 5 -0.05142705295326522 1101 6 0.03588976257594112 1101 7 0.10852566781776574 1101 8 0.08979555149363705 1101 9 0.06579071910754664 1101 10-11 0.16637256090375274 1101 12-13 0.2077013101627685 1101 14-15 0.28576490191260007 1101 16-17 0.29774172305235425 1101 18-19 0.29579232374421593 1101 20-21 0.21753147928998118 1101 22-23 0.22436254349764084 1101 24-25 0.26678942860095134 1101 26-27 0.2856907406864764 1101 28-29 0.28225458124700964 1101 30-31 0.30295345801715 1101 32-33 0.38324016089135426 1101 34-35 0.37190381515648596 1101 36-37 0.4119635176791405 1101 38-39 0.5402337526742969 1101 40-41 0.717785966864902 1101 42-43 0.6825351759915321 1101 44-45 0.6377753871630674 1101 46-47 0.640140668189396 1101 48-49 0.9604095665724373 1101 50-51 0.9346431270381146 1101 52-53 1.0681209337087196 1101 54-55 1.0453430789940725 1101 56-57 1.0818903245324591 1101 58-59 1.4621151346141303 1101 60-61 1.3580954342972902 1101 62-63 1.2817901118328692 1101 64-65 1.5252683724548035 1101 66-67 1.4590555806237333 1101 68-69 1.7387066399488056 1101 70-71 1.8734508225938278 1101 72-73 1.9924443123426911 1101 74-75 2.0516900323685725 1101 76-77 2.0160677440467616 1101 78-79 2.1248383505465434 1101 80-81 2.2581175720025577 1101 82-83 2.3581637697742437 1101 84-85 2.527269091042413 1101 86-87 2.7009952937063275 1101 88-89 3.106978071457309 1101 90-91 3.317898735254442 1101 92-93 3.4219934886357954 1101 94-95 3.669393230436093 1101 96-97 3.807420393519653 1101 98-99 4.126518623098196 1101 100-101 4.065084698180417 1102 1 0.19270461615762713 1102 2 0.14258230239422431 1102 3 0.21204535804146474 1102 4 0.12031544744905887 1102 5 0.00109645697140337 1102 6 0.05440061074092739 1102 7 0.07533000786972366 1102 8 0.1557349463857065 1102 9 0.14254723370471822 1102 10-11 0.2231820882687927 1102 12-13 0.28308711657381735 1102 14-15 0.38410044247385855 1102 16-17 0.3701377508280572 1102 18-19 0.34199890455823123 1102 20-21 0.2578887751279453 1102 22-23 0.38645900394241295 1102 24-25 0.36200177440610304 1102 26-27 0.2738778140594462 1102 28-29 0.19055310325575903 1102 30-31 0.3110124597737638 1102 32-33 0.2929353276516835 1102 34-35 0.2743561960153045 1102 36-37 0.33960796666467274 1102 38-39 0.3664923638105151 1102 40-41 0.3345148550900845 1102 42-43 0.4616918053383614 1102 44-45 0.31086561104278587 1102 46-47 0.4203190410235962 1102 48-49 0.5134690988497042 1102 50-51 0.6969956128221284 1102 52-53 0.7916391109183323 1102 54-55 0.8677746713027048 1102 56-57 0.8892849965331493 1102 58-59 1.0325480394701572 1102 60-61 0.8806445271069663 1102 62-63 1.1587295578121086 1102 64-65 1.0966013940253845 1102 66-67 1.0921467083534537 1102 68-69 1.4034213180799533 1102 70-71 1.3726204595212046 1102 72-73 1.5529517651754787 1102 74-75 1.5826127060354942 1102 76-77 1.5959625830632156 1102 78-79 1.6251959607671225 1102 80-81 1.7860288532295918 1102 82-83 1.8047845496789066 1102 84-85 2.240043831971491 1102 86-87 2.3054941088768643 1102 88-89 2.5319490893103094 1102 90-91 2.729420464987573 1102 92-93 3.003561629101945 1102 94-95 3.2397787814112675 1102 96-97 3.192052978491379 1102 98-99 3.345555669159179 1102 100-101 3.3674174171577285 1103 1 0.21029628100508546 1103 2 0.18201474627393566 1103 3 0.16636714732286606 1103 4 0.07126992615684458 1103 5 0.019716768308157384 1103 6 0.09776329399132777 1103 7 0.11286487605631379 1103 8 0.16080373117225832 1103 9 0.2034478451457744 1103 10-11 0.27054236829648204 1103 12-13 0.28046055088728394 1103 14-15 0.4290588991869271 1103 16-17 0.40074497504985374 1103 18-19 0.36849583429195576 1103 20-21 0.3496140763237747 1103 22-23 0.33652925342604334 1103 24-25 0.41378693226686636 1103 26-27 0.40376985562325984 1103 28-29 0.44862899508834886 1103 30-31 0.49582690971004695 1103 32-33 0.48257693400454826 1103 34-35 0.48977096650566665 1103 36-37 0.5593374361831138 1103 38-39 0.6833743502755922 1103 40-41 0.6361540097079867 1103 42-43 0.725012338898587 1103 44-45 0.8065652791069837 1103 46-47 0.9083091781080483 1103 48-49 1.023896156002749 1103 50-51 1.1658674415663839 1103 52-53 1.1321368653604722 1103 54-55 1.0477398544271566 1103 56-57 1.2600309844759892 1103 58-59 1.374599865490275 1103 60-61 1.3713492982877042 1103 62-63 1.3982231084803658 1103 64-65 1.459908493421409 1103 66-67 1.5290456927679585 1103 68-69 1.503937413887936 1103 70-71 1.6356232377809938 1103 72-73 1.9113857148010922 1103 74-75 1.923916417036768 1103 76-77 1.9352907255847676 1103 78-79 2.117510965795603 1103 80-81 2.168441199972836 1103 82-83 2.3057067490754903 1103 84-85 2.5266843020179692 1103 86-87 2.658069550105189 1103 88-89 2.9352956888807924 1103 90-91 3.116990597855189 1103 92-93 3.175563431518974 1103 94-95 3.153105472296751 1103 96-97 3.3010641307135202 1103 98-99 3.810089102502399 1103 100-101 3.5313087161107966 1104 1 0.1947714356558734 1104 2 0.18834086683141749 1104 3 0.17832084294634853 1104 4 0.08051408058859977 1104 5 0.03654715441518874 1104 6 0.09957132456979423 1104 7 0.07909821650638094 1104 8 0.11043918853990675 1104 9 0.1590940066348452 1104 10-11 0.23771522134006773 1104 12-13 0.2058429717239747 1104 14-15 0.2916427885583275 1104 16-17 0.3807524787135961 1104 18-19 0.26394772274854006 1104 20-21 0.23143154521224574 1104 22-23 0.23953066709970727 1104 24-25 0.3138898312652074 1104 26-27 0.346002699974747 1104 28-29 0.2287400368746333 1104 30-31 0.16977829688596557 1104 32-33 0.2906872302610779 1104 34-35 0.3425407581097204 1104 36-37 0.5009303790080963 1104 38-39 0.5746150147436779 1104 40-41 0.4953387083142857 1104 42-43 0.6427275147475626 1104 44-45 0.5660425411403054 1104 46-47 0.6078351206780681 1104 48-49 0.8310614984731401 1104 50-51 0.9494478050449615 1104 52-53 1.050950039334225 1104 54-55 1.1007663494077846 1104 56-57 1.0084377514540606 1104 58-59 1.2010507810961641 1104 60-61 1.1200849551311975 1104 62-63 1.1229989486177772 1104 64-65 1.2738184100726997 1104 66-67 1.4000737085187467 1104 68-69 1.60351797566776 1104 70-71 1.5981094966104088 1104 72-73 1.744438351689407 1104 74-75 1.7347889390485491 1104 76-77 1.720680156059455 1104 78-79 1.7023621327673197 1104 80-81 1.8046875405548732 1104 82-83 2.164431623720777 1104 84-85 2.359340854630325 1104 86-87 2.2989122301983045 1104 88-89 2.44634657836896 1104 90-91 2.822652996553998 1104 92-93 3.1245618375086437 1104 94-95 3.273721943875067 1104 96-97 3.5779627096467443 1104 98-99 3.9642452581668017 1104 100-101 3.6573418517647376 1105 1 0.19960290191914964 1105 2 0.1937160005959484 1105 3 0.20461458027937596 1105 4 0.1781242394224165 1105 5 0.06524004381822834 1105 6 0.05111093991467186 1105 7 0.14935121799739903 1105 8 0.18132418357196656 1105 9 0.21695489662970857 1105 10-11 0.1953378542340829 1105 12-13 0.2595519001154756 1105 14-15 0.4177046142352481 1105 16-17 0.3062461160500618 1105 18-19 0.3365734103842968 1105 20-21 0.3452414012503695 1105 22-23 0.3469894982369013 1105 24-25 0.36421096178749934 1105 26-27 0.3658084592151809 1105 28-29 0.38845775067785837 1105 30-31 0.4674078746938761 1105 32-33 0.519095977663909 1105 34-35 0.650960302334596 1105 36-37 0.4453310501584511 1105 38-39 0.4713996599153276 1105 40-41 0.5947820310570009 1105 42-43 0.6003090138541012 1105 44-45 0.6185510963303713 1105 46-47 0.5711351728446417 1105 48-49 0.6875214800809104 1105 50-51 0.697581319579534 1105 52-53 0.834987610744399 1105 54-55 0.9764643483287401 1105 56-57 0.9642809494561746 1105 58-59 1.1697959582392983 1105 60-61 1.2204448598283548 1105 62-63 1.1875228744519646 1105 64-65 1.1321404130139285 1105 66-67 1.4341886050720518 1105 68-69 1.6774022296539037 1105 70-71 1.7371887311265688 1105 72-73 1.8279632175422407 1105 74-75 1.787385501411837 1105 76-77 1.8152714456451449 1105 78-79 1.9184902125400853 1105 80-81 2.018356043042669 1105 82-83 2.1526463153066544 1105 84-85 2.3196512138398973 1105 86-87 2.3942697561508552 1105 88-89 2.5329276208585654 1105 90-91 2.6718131657828614 1105 92-93 2.8839400221885043 1105 94-95 3.121489782227574 1105 96-97 3.383912153989858 1105 98-99 3.598310953608415 1105 100-101 3.4899325980017935 1106 1 0.10824673346871094 1106 2 0.14541945746612583 1106 3 0.07777520937803217 1106 4 0.09627316491833682 1106 5 0.0148045506791874 1106 6 0.03886705403292723 1106 7 0.05894176055047495 1106 8 0.09316686818973352 1106 9 0.14888636028351243 1106 10-11 0.08699710390901316 1106 12-13 0.06124405297367019 1106 14-15 0.004185243807938832 1106 16-17 0.011155794867228508 1106 18-19 -0.06275608242108888 1106 20-21 -0.0883196481630577 1106 22-23 -0.09771261217959903 1106 24-25 0.002890981368956602 1106 26-27 -0.10727576144763873 1106 28-29 0.050649735343299085 1106 30-31 0.1377043345629616 1106 32-33 0.2672090888896079 1106 34-35 0.07786330388403684 1106 36-37 0.17755412389555403 1106 38-39 0.12250892686535053 1106 40-41 0.13766943732409942 1106 42-43 0.12048166136614213 1106 44-45 0.13814046290155346 1106 46-47 0.47303617644035256 1106 48-49 0.5387691907905818 1106 50-51 0.6909626990154081 1106 52-53 0.5850807472947324 1106 54-55 0.7381061892174827 1106 56-57 0.8810010721472494 1106 58-59 0.8892939659071857 1106 60-61 0.870250670880587 1106 62-63 0.7821161837531321 1106 64-65 0.9193815868444091 1106 66-67 1.0672072746202304 1106 68-69 1.0257418921197576 1106 70-71 1.0723027755410008 1106 72-73 1.29884019312345 1106 74-75 1.5045572983881321 1106 76-77 1.487732076840512 1106 78-79 1.7382648342431182 1106 80-81 1.6389084688927582 1106 82-83 1.87032238668845 1106 84-85 2.0527126684656807 1106 86-87 2.0862497875529122 1106 88-89 2.0860113522886152 1106 90-91 2.0050216333386786 1106 92-93 2.142981408546568 1106 94-95 2.497548127946196 1106 96-97 2.93668008718193 1106 98-99 3.149988520644701 1106 100-101 3.2084378581657553 1107 1 0.13951344873954952 1107 2 0.09972515953707983 1107 3 0.1901355065006456 1107 4 0.07244088634419654 1107 5 -0.06005483246038068 1107 6 0.013952103758157364 1107 7 0.06601803725205002 1107 8 0.07806331732388117 1107 9 0.11759082231081663 1107 10-11 0.16495682372808318 1107 12-13 0.1616470246852515 1107 14-15 0.3248099652517027 1107 16-17 0.22358752678090354 1107 18-19 0.13208735417943274 1107 20-21 0.05609983683290665 1107 22-23 0.04842394735460687 1107 24-25 0.20027119497855495 1107 26-27 0.1982184826588025 1107 28-29 0.23313066002412342 1107 30-31 0.1367336921534772 1107 32-33 0.1788040219193121 1107 34-35 0.08838381760773473 1107 36-37 0.18221733365837878 1107 38-39 0.09889683856351184 1107 40-41 0.12915354251421718 1107 42-43 0.38080906298743145 1107 44-45 0.40044320563951885 1107 46-47 0.45423080461687704 1107 48-49 0.35179526198629674 1107 50-51 0.3658191847149581 1107 52-53 0.3615803393776531 1107 54-55 0.4511408465443765 1107 56-57 0.5424330052130486 1107 58-59 0.49549394145339676 1107 60-61 0.6613972774018535 1107 62-63 0.6527727922070383 1107 64-65 0.7628660482224419 1107 66-67 0.9130730221157677 1107 68-69 1.0438636349648256 1107 70-71 0.9964131325086463 1107 72-73 1.1023000854236322 1107 74-75 1.120696251723576 1107 76-77 1.039704566026689 1107 78-79 0.9902862158335424 1107 80-81 1.0880600650788175 1107 82-83 1.4001696597422892 1107 84-85 1.4568661108216432 1107 86-87 1.5352757015890504 1107 88-89 1.6152158790911457 1107 90-91 1.7024111764017675 1107 92-93 2.0157134790814837 1107 94-95 2.1785387672428236 1107 96-97 2.5000866938913084 1107 98-99 2.873821375672879 1107 100-101 2.771765960937195 1108 1 0.25051083238987104 1108 2 0.24733289621845245 1108 3 0.16572427789176203 1108 4 0.15994767754243355 1108 5 0.03952499401871279 1108 6 0.13963330961536258 1108 7 0.161622347275582 1108 8 0.20485336592383163 1108 9 0.23341018849036743 1108 10-11 0.17007641371586146 1108 12-13 0.23506984422387944 1108 14-15 0.33673419680327754 1108 16-17 0.32808714758635205 1108 18-19 0.32475480704898274 1108 20-21 0.30218736494800424 1108 22-23 0.3243844035747827 1108 24-25 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-0.5950098454434496 2316 48-49 -0.4457918503549152 2316 50-51 -0.33768305075449945 2316 52-53 -0.311058224244519 2316 54-55 -0.034147635465416215 2316 56-57 0.1109483696300444 2316 58-59 0.1662767105968257 2316 60-61 0.2561315049229691 2316 62-63 0.16385078525224372 2316 64-65 0.36656699208732135 2316 66-67 0.5642404339389131 2316 68-69 0.8114315861393315 2316 70-71 0.8548440458575932 2316 72-73 0.8409879003409557 2316 74-75 1.183055204246461 2316 76-77 1.3037312497255051 2316 78-79 1.3078541670080526 2316 80-81 1.2542441542137261 2316 82-83 1.4670563249896098 2316 84-85 1.6814633714844796 2316 86-87 1.6842111923278225 2316 88-89 1.9679161842504698 2316 90-91 2.459369518606014 2316 92-93 2.489459132335334 2316 94-95 2.9457163983548824 2316 96-97 3.3356200130311855 2316 98-99 3.8579672844089536 2316 100-101 4.1769288308541626 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 6.0 4 21.0 5 37.0 6 109.0 7 276.0 8 680.0 9 1475.0 10 2441.0 11 4188.0 12 6445.0 13 9402.0 14 12428.0 15 17751.0 16 22878.0 17 32542.0 18 33454.0 19 27253.0 20 24974.0 21 24841.0 22 26167.0 23 28931.0 24 32714.0 25 36807.0 26 41374.0 27 48671.0 28 58474.0 29 70661.0 30 84810.0 31 103108.0 32 127227.0 33 159749.0 34 213403.0 35 295970.0 36 407475.0 37 481608.0 38 241018.0 39 10617.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.5117764422085 18.538514469761772 14.948053690925875 23.001655397103853 2 15.815036036890175 20.18143928197635 38.97724758840052 25.026277092732947 3 19.15715677683975 23.585208327834405 30.509506048323388 26.748128847002455 4 13.014439339420946 16.796526915254336 36.48694414714364 33.702089598181075 5 13.294232285237657 37.36266681157726 34.33294351389839 15.010157389286691 6 32.44781480356597 36.50749985037102 16.275654863759563 14.769030482303446 7 28.476175924882963 33.63441263142712 19.960155970898022 17.929255472791898 8 27.460099190217928 33.27894637252354 19.504063780328725 19.7568906569298 9 26.646726064679076 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22.839294138377518 34-35 22.67755992216908 26.131581251499945 29.408295012429935 21.78256381390104 36-37 22.745815519469645 25.237367932982142 29.687585677086677 22.32923087046154 38-39 21.828535461829404 25.816528560909944 29.660681247377813 22.694254729882836 40-41 22.417053294612913 25.729660717943837 29.709106078018106 22.144179909425148 42-43 22.046622035468882 25.878677868149293 29.213061456657456 22.86163863972437 44-45 21.989762753665726 26.02175893491298 29.85422974638121 22.134248565040085 46-47 22.984610717738995 26.691205800469813 28.504874926997843 21.819308554793345 48-49 22.310016881109924 26.754681896468725 28.23206711997707 22.703234102444288 50-51 22.400835096351695 26.963939257744173 28.662775200939482 21.972450444964643 52-53 23.210856996069502 26.324661550660615 28.380060290209364 22.08442116306052 54-55 21.98587057419194 26.84741461768059 28.741269945713533 22.425444862413933 56-57 22.433753446575434 26.921448377669943 28.18771749624255 22.457080679512075 58-59 22.40118825764715 26.33700360856494 28.10037513164552 23.161433002142388 60-61 21.444307021329827 26.939236554498926 28.86870541106302 22.747751013108232 62-63 21.828527923348386 26.970742259540835 28.960267123768908 22.24046269334187 64-65 21.244993938636924 27.296896009611938 28.477030946966696 22.981079104784445 66-67 21.24338472885381 27.002072128202805 28.581428616038433 23.173114526904953 68-69 22.27382351984461 26.36168719824161 28.76408128366102 22.600407998252763 70-71 22.079476904924146 26.88455231601319 27.812325626610367 23.223645152452296 72-73 21.82772866357446 26.940593437370936 28.21637932348422 23.015298575570384 74-75 21.86310055542978 27.739314175634178 27.790187989616317 22.60739727931973 76-77 22.033454411895363 26.812117075571685 28.32061766795329 22.83381084457966 78-79 22.18728403722097 27.293438746403798 28.073051599839253 22.44622561653598 80-81 22.714849551317535 27.28704636862557 27.589854523460524 22.40824955659637 82-83 22.28210611624291 27.330662257625903 27.920362165894808 22.46686946023638 84-85 22.003187895292044 27.72466994130741 27.898879589570097 22.373262573830445 86-87 22.64086621759198 27.749287473909362 27.03584127522885 22.574005033269806 88-89 22.03297113854369 27.96191731639051 26.730797040433995 23.274314504631803 90-91 22.002020147194898 27.664399050820794 27.14854401813002 23.185036783854287 92-93 22.407582335838548 27.116635049437004 27.478253628546433 22.997528986178008 94-95 21.849089689416886 26.624388914673798 27.943743997418576 23.582777398490737 96-97 22.135922691018724 26.122431909471615 28.01673615280383 23.724909246705835 98-99 21.789196907496645 26.910091453905572 27.137062642263594 24.163648996334185 100-101 21.943230994624884 25.785923288162145 27.864890897323374 24.405954819889597 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1123.0 1 797.5 2 679.0 3 878.0 4 704.0 5 677.0 6 1327.5 7 2963.0 8 4961.0 9 5665.0 10 4550.0 11 3360.5 12 3137.5 13 3313.0 14 3541.5 15 3667.5 16 3872.5 17 4195.5 18 4510.5 19 4961.0 20 5635.5 21 6695.0 22 8190.0 23 10049.5 24 12333.0 25 15502.0 26 19454.5 27 23493.0 28 27052.0 29 30447.5 30 34434.5 31 41629.5 32 54260.0 33 56235.5 34 49234.5 35 49225.5 36 53216.5 37 57682.5 38 60881.5 39 63384.0 40 67615.0 41 74210.0 42 77833.0 43 78944.0 44 80653.0 45 87767.5 46 91932.5 47 96558.5 48 103517.5 49 99153.5 50 92730.0 51 89174.5 52 87675.0 53 92049.5 54 98099.5 55 94245.5 56 86502.5 57 80538.5 58 76044.0 59 66844.0 60 50719.0 61 41780.0 62 37733.5 63 28191.5 64 18204.5 65 13145.0 66 9857.5 67 7717.0 68 6116.0 69 4720.0 70 3602.0 71 2539.5 72 1853.0 73 1442.5 74 1002.0 75 669.5 76 475.5 77 316.0 78 192.5 79 104.5 80 61.5 81 34.5 82 21.0 83 13.0 84 9.5 85 9.5 86 4.5 87 2.0 88 2.5 89 1.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014275151311027667 3 0.0026022411244060848 4 0.001710044167466856 5 0.12174770974899898 6 7.434974641160244E-5 7 0.0 8 2.230492392348073E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.858743660290061E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.788115490435091E-4 24-25 4.832733516754158E-4 26-27 0.006319728444986206 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011617147876812879 36-37 7.249100275131237E-4 38-39 0.007527911824174746 40-41 0.010408964497624339 42-43 0.0061896163887659025 44-45 0.003921949123212028 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.717487320580122E-5 68-69 9.851341399537321E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.434974641160244E-5 82-83 0.0022862547021567747 84-85 0.004702621460533853 86-87 0.0032528014055076063 88-89 0.0 90-91 0.0031970390956989046 92-93 0.0 94-95 1.858743660290061E-5 96-97 1.4869949282320487E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2689989.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.6596471920101 #Duplication Level Percentage of deduplicated Percentage of total 1 67.70883405808031 26.853084705258222 2 15.481864356749158 12.280105565264558 3 6.427951862143883 7.647909090595519 4 3.275557334105781 5.196297929113456 5 1.9038824945421189 3.7753654014292257 6 1.1845996471189855 2.8188482441111167 7 0.8213946217716076 2.2803354633413573 8 0.5746770292887408 1.8233190584755126 9 0.3951427485111104 1.410409979678863 >10 1.9918577975683929 14.037297591248423 >50 0.1386798404719606 3.7668050144348433 >100 0.07797766735135565 5.911410830061587 >500 0.008696757625459782 2.40906891392757 >1k 0.007948649442623327 6.818358184934525 >5k 7.481081828351367E-4 1.9706989990201955 >10k+ 1.8702704570878418E-4 1.0006850291051983 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15045 0.5592959673812793 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11937 0.4437564614576491 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8011 0.29780790925167355 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 7213 0.26814236043344414 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 7111 0.26435052336645243 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 7011 0.26063303604587235 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 6510 0.2420084245697659 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 6414 0.238439636742009 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5780 0.21487076712953102 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 5087 0.18910857999791078 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4935 0.18345799927062897 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4802 0.17851374113425741 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4748 0.17650629798114414 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 4311 0.16026087839020903 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4187 0.15565119411268968 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 4104 0.1525656796366082 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3745 0.13921990015572555 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3696 0.1373983313686413 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3601 0.13386671841409017 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 3542 0.1316734008949479 No Hit TCTTAGGAGTGGGGGTGGCTTTTGGGAGGGTGAGGGACTTCCTGTAACCA 3503 0.13022358083992167 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3480 0.12936855875618825 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3461 0.128662236165278 No Hit CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT 3362 0.12498192371790368 No Hit GGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTG 3261 0.12122726152411775 No Hit TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG 3225 0.1198889660887089 No Hit GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA 3215 0.1195172173566509 No Hit CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA 3128 0.11628300338774619 No Hit TTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCTCCCA 2979 0.11074394728008183 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2870 0.10669188610064949 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2866 0.10654318660782629 No Hit GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG 2837 0.10546511528485804 No Hit GCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTT 2834 0.10535359066524064 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.434974641160242E-5 2 1.1152461961740363E-4 0.0 0.0 0.0 7.434974641160242E-5 3 1.1152461961740363E-4 0.0 0.0 0.0 7.434974641160242E-5 4 1.1152461961740363E-4 0.0 0.0 0.0 7.434974641160242E-5 5 1.1152461961740363E-4 0.0 0.0 0.0 1.1152461961740363E-4 6 1.1152461961740363E-4 0.0 0.0 0.0 1.1152461961740363E-4 7 1.8587436602900606E-4 0.0 0.0 0.0 1.1152461961740363E-4 8 1.8587436602900606E-4 0.0 0.0 0.0 1.1152461961740363E-4 9 1.8587436602900606E-4 0.0 0.0 3.717487320580121E-5 1.1152461961740363E-4 10-11 1.8587436602900606E-4 0.0 0.0 3.717487320580121E-5 1.4869949282320485E-4 12-13 1.8587436602900606E-4 0.0 0.0 3.717487320580121E-5 5.762105346899188E-4 14-15 1.8587436602900606E-4 0.0 0.0 3.717487320580121E-5 6.505602811015213E-4 16-17 1.8587436602900606E-4 0.0 0.0 3.717487320580121E-5 7.249100275131237E-4 18-19 1.8587436602900606E-4 0.0 0.0 5.576230980870182E-5 7.620849007189249E-4 20-21 2.2304923923480727E-4 0.0 0.0 1.1152461961740363E-4 8.921969569392291E-4 22-23 2.602241124406085E-4 0.0 0.0 3.3457385885221093E-4 0.0010037215765566327 24-25 3.3457385885221093E-4 0.0 0.0 7.434974641160243E-4 0.0010223090131595333 26-27 3.717487320580121E-4 0.0 0.0 0.001654281857658154 0.001040896449762434 28-29 5.204482248812169E-4 0.0 0.0 0.005260244558620872 0.001040896449762434 30-31 6.319728444986206E-4 0.0 0.0 0.016133894971317727 0.001040896449762434 32-33 6.319728444986206E-4 0.0 0.0 0.04027897511848562 0.001040896449762434 34-35 6.691477177044219E-4 0.0 0.0 0.07531629311495326 0.001040896449762434 36-37 7.434974641160242E-4 1.8587436602900606E-5 0.0 0.13081837881121447 0.001040896449762434 38-39 8.178472105276267E-4 3.717487320580121E-5 0.0 0.21697114746565876 0.0010780713229682352 40-41 8.178472105276267E-4 3.717487320580121E-5 0.0 0.30509418440001057 0.0010780713229682352 42-43 8.55022083733428E-4 3.717487320580121E-5 0.0 0.3969718835281483 0.0010966587595711357 44-45 9.293718301450303E-4 3.717487320580121E-5 0.0 0.5115262553118247 0.0011524210693798376 46-47 0.0010966587595711357 3.717487320580121E-5 0.0 0.6403557784065288 0.0011895959425856388 48-49 0.0011524210693798376 3.717487320580121E-5 0.0 0.7722708159773144 0.0011895959425856388 50-51 0.0011524210693798376 3.717487320580121E-5 0.0 0.9169368350576899 0.0013382954354088437 52-53 0.0011710085059827382 3.717487320580121E-5 0.0 1.0555805246787253 0.0015241698014378498 54-55 0.0012267708157914401 3.717487320580121E-5 0.0 1.1988153111406774 0.0016356944210552532 56-57 0.0012639456889972412 3.717487320580121E-5 0.0 1.3630539009639073 0.001710044167466856 58-59 0.0012639456889972412 3.717487320580121E-5 0.0 1.5243928506770845 0.001747219040672657 60-61 0.0012639456889972412 3.717487320580121E-5 0.0 1.7065125545123045 0.0018029813504813587 62-63 0.0012639456889972412 3.717487320580121E-5 0.0 1.880509548552057 0.0019702682799074642 64-65 0.0012639456889972412 3.717487320580121E-5 0.0 2.055064165689897 0.0020074431531132654 66-67 0.0012639456889972412 3.717487320580121E-5 0.0 2.266775068596935 0.0020074431531132654 68-69 0.0012639456889972412 3.717487320580121E-5 0.0 2.4942852926164383 0.0020074431531132654 70-71 0.0012639456889972412 3.717487320580121E-5 0.0 2.725104080351258 0.0020074431531132654 72-73 0.0012639456889972412 3.717487320580121E-5 0.0 2.971015866607633 0.002174730082539371 74-75 0.0012639456889972412 3.717487320580121E-5 0.0 3.213210165543428 0.0023420170119654764 76-77 0.0012639456889972412 3.717487320580121E-5 0.0 3.47114802328188 0.0023791918851712775 78-79 0.0012639456889972412 3.717487320580121E-5 0.0 3.767468937605321 0.00245354163158288 80-81 0.0013382954354088437 5.576230980870182E-5 0.0 4.067990612601018 0.002509303941391582 82-83 0.0014126451818204462 7.434974641160242E-5 0.0 4.3763561858431395 0.0026022411244060848 84-85 0.0014498200550262473 7.434974641160242E-5 0.0 4.69894114808648 0.0026022411244060848 86-87 0.0014869949282320485 7.434974641160242E-5 0.0 5.02479749917193 0.0027137657440234886 88-89 0.0014869949282320485 7.434974641160242E-5 0.0 5.361044227318402 0.0028252903636408924 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16890 0.0 30.498499 1 AGGTAAG 2265 0.0 30.449448 2 GGTATCA 11715 0.0 29.963581 1 TAAGGTG 2740 0.0 28.289661 5 AAGGTAA 2375 0.0 27.837536 1 GTGTAAG 1170 0.0 27.237585 1 TGTAAGG 1240 0.0 26.08356 2 TAAGGTA 1225 0.0 26.0108 4 GTAAGGT 2900 0.0 25.746408 4 TATCAAC 20295 0.0 25.33473 2 ATCAACG 20460 0.0 25.057877 3 TCAACGC 20595 0.0 24.892694 4 CAACGCA 20610 0.0 24.87319 5 GGTAAGG 3005 0.0 24.689442 3 AACGCAG 21145 0.0 24.325071 6 GTACATG 8670 0.0 22.986591 1 TACATGG 8440 0.0 22.880379 2 GAGTACT 13325 0.0 22.828915 12-13 AGAGTAC 17400 0.0 22.368305 10-11 AAGGTGT 3680 0.0 22.068949 6 >>END_MODULE