##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937970_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2669690 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53589517884099 34.0 31.0 34.0 31.0 34.0 2 32.756298296805994 34.0 31.0 34.0 31.0 34.0 3 32.71263255284321 34.0 31.0 34.0 31.0 34.0 4 36.23364285741041 37.0 35.0 37.0 35.0 37.0 5 36.201641389075135 37.0 37.0 37.0 35.0 37.0 6 36.26550685660133 37.0 37.0 37.0 35.0 37.0 7 36.22637085204649 37.0 37.0 37.0 35.0 37.0 8 36.216276421607006 37.0 37.0 37.0 35.0 37.0 9 37.93627162704284 39.0 38.0 39.0 35.0 39.0 10-11 37.9022927755657 39.0 38.0 39.0 35.0 39.0 12-13 37.851919323966456 39.0 38.0 39.0 35.0 39.0 14-15 39.133336267506714 40.0 38.5 41.0 36.0 41.0 16-17 39.08001472081028 40.0 38.0 41.0 36.0 41.0 18-19 39.0939622203327 40.0 38.5 41.0 36.0 41.0 20-21 39.098450943742534 40.0 39.0 41.0 36.0 41.0 22-23 39.12222767437418 40.0 39.0 41.0 35.5 41.0 24-25 39.05453985294173 40.0 39.0 41.0 35.0 41.0 26-27 38.8753388970255 40.0 38.0 41.0 35.0 41.0 28-29 38.7965304960501 40.0 38.0 41.0 35.0 41.0 30-31 38.548676999951304 40.0 38.0 41.0 34.5 41.0 32-33 38.30389052661545 40.0 38.0 41.0 34.0 41.0 34-35 38.07361959628271 40.0 38.0 41.0 34.0 41.0 36-37 37.9503281279849 40.0 37.5 41.0 33.0 41.0 38-39 37.855139922612736 40.0 37.0 41.0 33.0 41.0 40-41 37.71739977300735 40.0 37.0 41.0 33.0 41.0 42-43 37.503810929358835 40.0 37.0 41.0 33.0 41.0 44-45 37.24306436327814 40.0 37.0 41.0 32.0 41.0 46-47 36.85632564080474 40.0 36.0 41.0 31.0 41.0 48-49 36.420418662841 39.5 35.5 41.0 30.0 41.0 50-51 36.07670178934633 39.0 35.0 41.0 29.5 41.0 52-53 35.6018769969547 39.0 35.0 40.0 28.0 41.0 54-55 35.26728777498511 38.5 35.0 40.0 27.0 41.0 56-57 34.95926455880645 38.0 34.5 40.0 26.5 41.0 58-59 34.56010922616484 38.0 34.0 40.0 26.0 41.0 60-61 34.12301428255715 37.0 34.0 40.0 25.0 41.0 62-63 33.69789544853522 36.5 33.0 39.5 23.5 41.0 64-65 33.25765444677097 36.0 33.0 39.0 22.5 40.5 66-67 32.75086995119284 35.5 32.5 39.0 21.5 40.0 68-69 32.22361791069375 35.0 32.0 38.0 19.5 40.0 70-71 31.616593874195132 35.0 31.0 37.5 17.5 39.5 72-73 31.17053047357558 35.0 31.0 37.0 14.5 39.0 74-75 30.65693526214654 34.0 30.0 36.0 10.0 39.0 76-77 29.446551472268318 33.5 28.5 35.0 9.0 37.0 78-79 30.12985852289966 34.0 30.0 35.0 7.5 37.0 80-81 29.968733635740477 34.0 30.0 35.0 6.0 37.0 82-83 29.533394326682124 34.0 29.5 35.0 2.0 36.5 84-85 29.070959924185953 34.0 29.0 35.0 2.0 36.0 86-87 28.59337526079807 34.0 29.0 35.0 2.0 36.0 88-89 27.989751244526516 33.0 27.5 35.0 2.0 35.0 90-91 27.49293569665392 33.0 26.5 35.0 2.0 35.0 92-93 26.924283531046676 33.0 25.5 35.0 2.0 35.0 94-95 26.227632421741852 32.0 24.5 34.5 2.0 35.0 96-97 25.460731021204708 32.0 21.5 34.0 2.0 35.0 98-99 24.483728260584563 32.0 10.0 34.0 2.0 35.0 100-101 20.93872603186138 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.025380071589538034 1101 2 0.010858405790564518 1101 3 0.07950752640967806 1101 4 0.02700569593847746 1101 5 -0.030827795275321535 1101 6 0.045369211618499605 1101 7 0.02335277995226903 1101 8 0.033097838027423165 1101 9 0.026947306114962544 1101 10-11 0.08386553600782776 1101 12-13 0.060583516420457784 1101 14-15 0.1408862824533088 1101 16-17 0.16530512123256358 1101 18-19 0.1806026473498079 1101 20-21 0.13379434480977892 1101 22-23 0.0671886535665962 1101 24-25 0.15123348993314067 1101 26-27 0.18709184902710518 1101 28-29 0.17735630820067882 1101 30-31 0.04128354043061222 1101 32-33 0.1037694021587825 1101 34-35 0.056204575095641474 1101 36-37 0.17481364013359268 1101 38-39 0.19535980564328526 1101 40-41 0.21379286067510606 1101 42-43 0.16505074260388852 1101 44-45 0.1968110033108701 1101 46-47 0.2137506100371951 1101 48-49 0.1889769830561363 1101 50-51 0.2668758650761234 1101 52-53 0.23184600983451986 1101 54-55 0.2584085948752062 1101 56-57 0.27415767413418024 1101 58-59 0.2551846423166282 1101 60-61 0.1917383090745588 1101 62-63 0.19522827896435757 1101 64-65 0.18753324224267232 1101 66-67 0.16408408717651923 1101 68-69 0.2636669983216464 1101 70-71 0.33285072790170034 1101 72-73 0.4417857005081629 1101 74-75 0.5928159606801309 1101 76-77 0.5842110718558686 1101 78-79 0.6147856413244419 1101 80-81 0.8083366552350846 1101 82-83 0.8230927936147232 1101 84-85 0.8351237813620997 1101 86-87 0.9722543647919011 1101 88-89 1.168854693398277 1101 90-91 1.2539769745108131 1101 92-93 1.439899963665436 1101 94-95 1.4055526138112278 1101 96-97 1.6095556623302691 1101 98-99 1.738713044941246 1101 100-101 2.280146452037208 1102 1 0.10624997990548479 1102 2 0.11147724722802366 1102 3 0.269827226541544 1102 4 0.10743950793943213 1102 5 0.03761855968630812 1102 6 0.07473822178369716 1102 7 0.04670989091801658 1102 8 0.09720399633038568 1102 9 0.06505900907107076 1102 10-11 0.14697230692227947 1102 12-13 0.1912644496665692 1102 14-15 0.182709995709601 1102 16-17 0.16678732954123632 1102 18-19 0.2304448220167714 1102 20-21 0.18133143895466475 1102 22-23 0.224219668321183 1102 24-25 0.11572019418715485 1102 26-27 0.31018262974334476 1102 28-29 0.12303895468230763 1102 30-31 0.14144292325049435 1102 32-33 0.20467637866149602 1102 34-35 0.14009600787970555 1102 36-37 0.1736818663637436 1102 38-39 0.2777923924212615 1102 40-41 0.3712911214814696 1102 42-43 0.33892597752482345 1102 44-45 0.33125067146234244 1102 46-47 0.42954300742476903 1102 48-49 0.4284976924394712 1102 50-51 0.48987683071796795 1102 52-53 0.4947445966747992 1102 54-55 0.571658135416591 1102 56-57 0.5803907364647998 1102 58-59 0.5954410046503682 1102 60-61 0.7093067606211392 1102 62-63 0.5734379997588803 1102 64-65 0.637504169155001 1102 66-67 0.7071511940927948 1102 68-69 0.6697375628587068 1102 70-71 0.769912684728169 1102 72-73 0.7411580333119474 1102 74-75 0.7952477167984568 1102 76-77 0.9455428269358741 1102 78-79 1.025089974252829 1102 80-81 1.1174510682182976 1102 82-83 1.1498509366750795 1102 84-85 1.1025520590931528 1102 86-87 0.9619074605565778 1102 88-89 1.1413287093261744 1102 90-91 1.3238847712899329 1102 92-93 1.4653103614475746 1102 94-95 1.4398666446909978 1102 96-97 1.7400107599846955 1102 98-99 1.798276533296434 1102 100-101 2.0888974514245966 1103 1 0.2771969541535455 1103 2 0.273747629168291 1103 3 0.3641917385364195 1103 4 0.1573347113249639 1103 5 0.08728097441390759 1103 6 0.1015139236540179 1103 7 0.18388081619869467 1103 8 0.1872439792425311 1103 9 0.17961105360629404 1103 10-11 0.28238751344503754 1103 12-13 0.23967948430952646 1103 14-15 0.4169773622441042 1103 16-17 0.3852144291447388 1103 18-19 0.36082086330797125 1103 20-21 0.32706047629429946 1103 22-23 0.3942313160977662 1103 24-25 0.4496317445095528 1103 26-27 0.35440183655979496 1103 28-29 0.2999616721853542 1103 30-31 0.4188302168278639 1103 32-33 0.4140524633398286 1103 34-35 0.38482655709406544 1103 36-37 0.3212066721228908 1103 38-39 0.28559957332558383 1103 40-41 0.4070779972073666 1103 42-43 0.3445838265909984 1103 44-45 0.5850793773910254 1103 46-47 0.6039670015107674 1103 48-49 0.5639817343180624 1103 50-51 0.5126139831202821 1103 52-53 0.4965207574474846 1103 54-55 0.5906443867015128 1103 56-57 0.6767216451248714 1103 58-59 0.6940929163542151 1103 60-61 0.7668272526979862 1103 62-63 0.7143948696108353 1103 64-65 0.6724850454415972 1103 66-67 0.7404862795988265 1103 68-69 0.8173287008698438 1103 70-71 0.8702669156411673 1103 72-73 0.676405865624016 1103 74-75 0.7960445381903156 1103 76-77 0.3330356096531091 1103 78-79 0.7375649014959436 1103 80-81 0.8867068203509412 1103 82-83 0.952783239397121 1103 84-85 1.0953779548705853 1103 86-87 1.0882820817535261 1103 88-89 1.3419289119107134 1103 90-91 1.3765452786523937 1103 92-93 1.697420261056422 1103 94-95 1.5509089204462683 1103 96-97 1.5587893788053684 1103 98-99 2.1665356746316924 1103 100-101 2.5177219020767367 1104 1 0.08676838272497633 1104 2 0.06750159742226458 1104 3 0.12754888139356524 1104 4 0.06984264783289973 1104 5 -0.0040920414591099075 1104 6 0.043013923654015684 1104 7 -0.027301723483844853 1104 8 -0.007248084249532383 1104 9 0.06580152979677223 1104 10-11 0.07934981503233729 1104 12-13 0.06629853192857382 1104 14-15 0.11200712414886738 1104 16-17 0.1391549053352179 1104 18-19 0.08035062521273062 1104 20-21 0.1475207937546159 1104 22-23 0.10285830022474585 1104 24-25 0.07013174450955972 1104 26-27 0.15707445560742173 1104 28-29 0.030374370598046596 1104 30-31 0.09381434381199227 1104 32-33 0.1878163522287153 1104 34-35 0.1416797316972378 1104 36-37 0.19809357688479423 1104 38-39 0.1745757638017693 1104 40-41 0.23981609244545865 1104 42-43 0.2707346202417895 1104 44-45 0.2552480281846812 1104 46-47 0.2895285094472797 1104 48-49 0.2442892740006073 1104 50-51 0.42063977677107545 1104 52-53 0.43478464633637515 1104 54-55 0.43225351368565157 1104 56-57 0.41253315306138205 1104 58-59 0.3647318052431032 1104 60-61 0.5095336019043302 1104 62-63 0.464819472785436 1104 64-65 0.4729850454415967 1104 66-67 0.5687362795988307 1104 68-69 0.7499656056317434 1104 70-71 0.7566339791332268 1104 72-73 0.7859951513382981 1104 74-75 0.7675762842220628 1104 76-77 0.7014741017166024 1104 78-79 0.8825192665753079 1104 80-81 1.001117534636652 1104 82-83 0.9696086362225174 1104 84-85 1.231125970743598 1104 86-87 1.2496670023884455 1104 88-89 1.4116451817519824 1104 90-91 1.3208925008746135 1104 92-93 1.4883210547072174 1104 94-95 1.6132144760018257 1104 96-97 2.0159639819799686 1104 98-99 2.278321388917405 1104 100-101 2.5947596004894358 1105 1 0.13192283778305836 1105 2 0.11766976719297162 1105 3 0.18862762981486014 1105 4 0.07653471606848683 1105 5 0.10768368474470691 1105 6 0.06531379735770315 1105 7 0.09195985219611202 1105 8 0.028818443669074156 1105 9 0.07742221393146309 1105 10-11 0.04287486973109367 1105 12-13 0.06041653893713317 1105 14-15 0.12739108273790123 1105 16-17 0.1421268177460604 1105 18-19 0.16235137231769414 1105 20-21 0.13441486494304655 1105 22-23 0.21015851190448842 1105 24-25 0.20746856433274274 1105 26-27 0.157431002559413 1105 28-29 0.21780087859385588 1105 30-31 0.1658403146037486 1105 32-33 0.21420345933155005 1105 34-35 0.2355802955836097 1105 36-37 0.29195756820414687 1105 38-39 0.20003048390422862 1105 40-41 0.3485180865041997 1105 42-43 0.39964687098444784 1105 44-45 0.3677278830328561 1105 46-47 0.33471227948125204 1105 48-49 0.38048473089087054 1105 50-51 0.5352499480715665 1105 52-53 0.5146985513828923 1105 54-55 0.5372607534885532 1105 56-57 0.4856577950735854 1105 58-59 0.5896496059083844 1105 60-61 0.5056655192958672 1105 62-63 0.5786609798028906 1105 64-65 0.72233317856859 1105 66-67 0.8710856756889811 1105 68-69 0.7662373383816217 1105 70-71 0.9228489674819542 1105 72-73 0.7489247989537944 1105 74-75 0.7706981868493799 1105 76-77 0.6998898122667896 1105 78-79 0.8859179538051478 1105 80-81 0.9624437526845746 1105 82-83 0.9082385880164594 1105 84-85 1.0065815091038814 1105 86-87 1.1941584551518467 1105 88-89 1.2198210716073667 1105 90-91 1.170764461313631 1105 92-93 1.5077925313068228 1105 94-95 1.5444097372217378 1105 96-97 1.814164383993596 1105 98-99 2.1986600765030104 1105 100-101 2.4082342433910497 1106 1 -0.032765589719886634 1106 2 0.02286688633210332 1106 3 0.11901454224535968 1106 4 0.033703300564837946 1106 5 0.011992195536016936 1106 6 -0.06588942382358454 1106 7 -0.0441922887308408 1106 8 -0.03968645189581821 1106 9 -0.059518811761194 1106 10-11 -0.00858585472511919 1106 12-13 -0.05274678212663986 1106 14-15 0.0050753833557379835 1106 16-17 0.0023286703307690004 1106 18-19 -0.04802634581771059 1106 20-21 -0.0463286483324481 1106 22-23 -0.03851463706898528 1106 24-25 -0.018029604958719858 1106 26-27 -0.016190356588090538 1106 28-29 -0.04748973962249892 1106 30-31 -0.08900151872663997 1106 32-33 0.026213753873648216 1106 34-35 -0.13951173983086562 1106 36-37 2.1437944123903208E-4 1106 38-39 0.14484115224537675 1106 40-41 0.18956725803820973 1106 42-43 0.010481071077350634 1106 44-45 0.23113780763881664 1106 46-47 -0.13226770826973677 1106 48-49 0.07563193207593599 1106 50-51 0.25348487080427873 1106 52-53 0.042953748960925964 1106 54-55 0.055160109107092126 1106 56-57 0.21626912665874443 1106 58-59 0.20067229453012914 1106 60-61 0.22043600643049643 1106 62-63 0.1794811103777647 1106 64-65 0.2520498471598671 1106 66-67 0.28287489346021744 1106 68-69 -0.10789410414875533 1106 70-71 0.1951030450874427 1106 72-73 0.2645415393104322 1106 74-75 0.3482964895759295 1106 76-77 0.29622039539358624 1106 78-79 0.5753207752976088 1106 80-81 0.6056066121158317 1106 82-83 0.5645753709912356 1106 84-85 0.6975531277450138 1106 86-87 0.9172657241653859 1106 88-89 1.0355179880802332 1106 90-91 0.9862329587299286 1106 92-93 0.9830445080049266 1106 94-95 1.2987070633557956 1106 96-97 1.3872638929234462 1106 98-99 1.6490877988917383 1106 100-101 2.269130599494101 1107 1 0.0995658230735259 1107 2 0.07737082374038096 1107 3 0.1539884470697288 1107 4 0.08824341028226712 1107 5 0.0456391270626213 1107 6 0.06401477460197214 1107 7 0.01532859068614556 1107 8 0.0706314895957334 1107 9 0.0390089228326147 1107 10-11 0.05832990285016848 1107 12-13 0.08059050919124644 1107 14-15 0.07609984343815057 1107 16-17 0.14127424227656604 1107 18-19 0.10940137574882414 1107 20-21 0.03642062016123049 1107 22-23 0.11002675388211713 1107 24-25 0.0038520208974475167 1107 26-27 0.06291120975153319 1107 28-29 0.09074477122434388 1107 30-31 0.1499618641496454 1107 32-33 0.004161292492177893 1107 34-35 0.1392901336850514 1107 36-37 0.12624545407598475 1107 38-39 0.1516412187185523 1107 40-41 0.14837492698713817 1107 42-43 0.08122748929792323 1107 44-45 0.04314448483739852 1107 46-47 0.23642431937953745 1107 48-49 0.31650071754872044 1107 50-51 0.3513208755150714 1107 52-53 0.32284210234237065 1107 54-55 0.3332271342990083 1107 56-57 0.35736708205828194 1107 58-59 0.37408365520396103 1107 60-61 0.31190692979159707 1107 62-63 0.3531593073611532 1107 64-65 0.2486342506562096 1107 66-67 0.3767815500300955 1107 68-69 0.5078990274636652 1107 70-71 0.6539380398568753 1107 72-73 0.4373666071400635 1107 74-75 0.5337816690207262 1107 76-77 0.6761901233647158 1107 78-79 0.7782208901840093 1107 80-81 0.821668336229628 1107 82-83 1.0481347943626886 1107 84-85 1.149671802800512 1107 86-87 1.240720544033838 1107 88-89 1.234707164801101 1107 90-91 1.209432750712935 1107 92-93 1.4286572131877655 1107 94-95 1.4399903363102062 1107 96-97 1.4550644959525378 1107 98-99 1.623658534497583 1107 100-101 2.2797488104693535 1108 1 0.11112885093460534 1108 2 0.10735807520890717 1108 3 0.20489072762926241 1108 4 0.09360987315878333 1108 5 0.07707768453184372 1108 6 0.07341469513579568 1108 7 0.09443437654275755 1108 8 0.09588546190636293 1108 9 0.07240168941368808 1108 10-11 0.07957287967719395 1108 12-13 0.10462029303525355 1108 14-15 0.2481783132949147 1108 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2316 96-97 -3.0349360106827348 2316 98-99 -3.49451887816015 2316 100-101 -3.8143644731770117 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 6.0 4 18.0 5 42.0 6 93.0 7 275.0 8 680.0 9 1281.0 10 2293.0 11 3804.0 12 5857.0 13 8233.0 14 11581.0 15 15808.0 16 20032.0 17 29345.0 18 29465.0 19 22570.0 20 19165.0 21 18081.0 22 18240.0 23 19932.0 24 22332.0 25 25419.0 26 29046.0 27 35275.0 28 42108.0 29 50827.0 30 62349.0 31 76921.0 32 97043.0 33 129192.0 34 184206.0 35 280416.0 36 435478.0 37 588376.0 38 360125.0 39 23770.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.548164768194056 18.533163026418798 14.93686532893332 22.98180687645382 2 15.790067157740708 20.26753707873755 38.946881812191336 24.995513951330402 3 19.411095939929613 23.60999200235247 30.35888272676702 26.620029330950896 4 13.032936408347037 16.808730601680345 36.52712487217617 33.63120811779645 5 13.347809507827549 37.38029437302083 34.29107823651877 14.980817882632861 6 32.42387488556698 36.482744774287895 16.310244957830214 14.78313538231491 7 28.43142836808768 33.69780761062145 19.947971487326242 17.922792533964618 8 27.471882461083435 33.284925642940784 19.505177762478436 19.738014133497348 9 26.635826631556476 16.41917226344632 20.017904700545756 36.927096404451454 10-11 25.089523502728785 26.231304009079704 26.713082792384135 21.966089695807376 12-13 26.720742857784984 23.731144814566484 27.142083912364356 22.406028415284172 14-15 22.658267439290704 25.154100288797576 25.997194430814062 26.19043784109766 16-17 21.81949317307993 28.30429467707562 27.363286016327372 22.512926133517077 18-19 22.05836632717656 26.175791945881354 30.086152324801755 21.679689402140323 20-21 22.036528585715946 25.221280373376686 30.757634781566402 21.984556259340973 22-23 22.04212848682806 25.230438740078437 30.92064996310433 21.806782809989176 24-25 22.162946658922802 25.588014566006173 30.554357412543 21.694681362528026 26-27 21.173051961614988 25.875135742584295 30.542170446415845 22.409641849384883 28-29 21.562450251621446 26.867308315400507 30.0457544616687 21.524486971309347 30-31 21.466390229558918 25.89009564798843 29.81464105315796 22.82887306929469 32-33 21.07144870552729 26.025579482772798 30.03507453643345 22.86789727526647 34-35 22.637429083769895 26.098952199646458 29.483450179141514 21.78016853744214 36-37 22.700912390165545 25.252240892581007 29.770344098008895 22.276502619244553 38-39 21.800458951903714 25.84455580012176 29.653219688100034 22.70176555987449 40-41 22.327159251886712 25.754881585696243 29.758892734186293 22.15906642823075 42-43 22.009847134144536 25.891211721251622 29.236423756454645 22.862517388149197 44-45 21.94030233744263 26.008710833699052 29.933942938101683 22.117043890756637 46-47 22.966208943460817 26.73756047896588 28.514775381499994 21.781455196073306 48-49 22.250321714527264 26.748949921160982 28.327284856745965 22.673443507565793 50-51 22.365967584251358 26.98337634706651 28.716648749480278 21.934007319201854 52-53 23.19705658709438 26.3548951376377 28.434387513156956 22.013660762110955 54-55 21.957174803067023 26.854391333825276 28.778472406908666 22.409961456199035 56-57 22.422397356996505 26.948840502080767 28.25228771879881 22.37647442212392 58-59 22.371942060688692 26.313448377901555 28.17014334997696 23.14446621143279 60-61 21.4494379497245 26.90922916143822 28.93628099142597 22.70505189741131 62-63 21.82062336825624 26.94037509973068 29.066595747071755 22.172405784941322 64-65 21.206132547224584 27.26767901891231 28.61219842004128 22.91399001382183 66-67 21.21986699177375 26.992234475646843 28.66063386890848 23.127264663670932 68-69 22.2099902385524 26.37697841618827 28.83597546677774 22.577055878481588 70-71 22.01609550172492 26.89724275103102 27.88252568650293 23.204136060741135 72-73 21.81762676565444 26.922882432042673 28.260247444459846 22.999243357843046 74-75 21.787904213597834 27.789705920912166 27.85797227393443 22.564417591555575 76-77 22.056118875225213 26.854522435189104 28.23799767014148 22.851361019444205 78-79 22.12665140896509 27.339747311485603 28.0961272657125 22.437474013836813 80-81 22.697571418966234 27.299628626806932 27.600431736085866 22.40236821814097 82-83 22.244480495522104 27.36425377078582 27.943183218593763 22.448082515098314 84-85 21.93982196928336 27.811273280914655 27.902223389273768 22.346681360528216 86-87 22.585855407628824 27.799481398034033 27.098610225392395 22.51605296894475 88-89 22.01863213280648 28.01258873224559 26.756861941596945 23.21191719335099 90-91 21.91860614298137 27.747738939867194 27.13592432123245 23.197730595918976 92-93 22.384526926548975 27.205141459431314 27.523233208598814 22.887098405420893 94-95 21.849305293612854 26.68765912433995 27.9814615110599 23.481574070987296 96-97 22.132554813207047 26.10911680255371 28.135108302285612 23.623220081953626 98-99 21.78031349432507 26.97647640739167 27.19899358781188 24.04421651047138 100-101 21.807041284081492 26.169359785362108 27.88035716794104 24.14324176261536 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1207.0 1 852.5 2 689.5 3 903.0 4 739.0 5 678.0 6 1380.0 7 3090.0 8 5068.0 9 5804.0 10 4666.0 11 3366.5 12 3216.0 13 3381.0 14 3529.5 15 3725.5 16 3941.5 17 4221.5 18 4527.0 19 4984.0 20 5573.5 21 6563.5 22 7964.0 23 9806.0 24 12294.5 25 15201.5 26 18906.5 27 23178.5 28 26852.0 29 30112.5 30 33962.5 31 41086.5 32 54191.5 33 56431.5 34 48871.0 35 48740.0 36 52906.0 37 57690.0 38 61096.0 39 63264.0 40 67326.0 41 73957.5 42 77359.5 43 78478.0 44 79973.0 45 87096.5 46 91253.0 47 96147.0 48 103677.5 49 98796.0 50 91978.5 51 88582.5 52 87151.5 53 91423.0 54 97991.0 55 94064.5 56 85725.0 57 79917.0 58 75428.0 59 65890.0 60 49662.0 61 40973.5 62 37312.5 63 27292.5 64 16986.5 65 12519.0 66 9366.0 67 7297.5 68 5907.0 69 4627.0 70 3498.0 71 2371.5 72 1683.0 73 1281.5 74 922.0 75 644.5 76 423.5 77 260.5 78 163.5 79 93.5 80 49.5 81 31.5 82 17.0 83 11.5 84 7.5 85 4.0 86 4.0 87 4.5 88 2.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011349632354318292 3 0.0058059175409879045 4 0.0 5 5.993205203600418E-4 6 2.2474519513501567E-4 7 0.0 8 1.4983013009001045E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.618629878375392E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0018541478598638793 26-27 0.021556809966700254 28-29 1.8728766261251307E-4 30-31 1.8728766261251307E-4 32-33 0.0033524491607639837 34-35 0.016162925283459875 36-37 0.008165742089905571 38-39 0.021163505875213977 40-41 0.023560787956654144 42-43 0.018241818338458775 44-45 0.009570399559499418 46-47 0.0023785533151789158 48-49 3.5584655896377484E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.0601642887376436E-4 68-69 1.4983013009001045E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.3110136382875914E-4 82-83 7.866081829725549E-4 84-85 0.0035397368233764967 86-87 4.6821915653128264E-4 88-89 3.745753252250261E-5 90-91 0.0033899066932864866 92-93 7.491506504500523E-4 94-95 3.745753252250261E-5 96-97 3.5584655896377484E-4 98-99 3.183890264412722E-4 100-101 0.0029029587704939522 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2669690.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.64971748128532 #Duplication Level Percentage of deduplicated Percentage of total 1 67.40738108188448 26.726836160500568 2 15.780316143170076 12.51370153684119 3 6.4612517767341675 7.685604225688877 4 3.2821918734600595 5.205519220082478 5 1.905628852659834 3.7778822816074165 6 1.1859430331260192 2.8213383727407155 7 0.7780165425440642 2.1593695275346985 8 0.5579078164791408 1.769670984319897 9 0.4042062758752082 1.4423998178353035 >10 2.0062096528628235 14.124317643744755 >50 0.13634168187758117 3.7336136313894075 >100 0.07700423100908174 5.879854194217568 >500 0.008800519158895357 2.45401035718829 >1k 0.007948856014485197 6.888901026397328 >5k 6.624046678737664E-4 1.7953427962915613 >10k+ 1.892584765353618E-4 1.0216382236201238 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15197 0.5692421217444722 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12032 0.45068903131075144 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8172 0.30610295577389135 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 7249 0.27152965325562145 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6999 0.2621652701249958 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6967 0.2609666290842757 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 6458 0.24190074503032186 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 6370 0.23860448216834165 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5635 0.21107319576430225 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4957 0.18567698871404545 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4752 0.17799819454693241 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4731 0.17721158636395984 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4690 0.17567582753053723 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 4355 0.16312755413549887 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4007 0.15009233281766798 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3926 0.14705827268334526 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3578 0.13402305136551435 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 3557 0.1332364431825418 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3525 0.1320378021418217 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3479 0.1303147556457866 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3445 0.1290411995400215 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3401 0.1273930681090314 No Hit TCTTAGGAGTGGGGGTGGCTTTTGGGAGGGTGAGGGACTTCCTGTAACCA 3357 0.1257449366780413 No Hit CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT 3288 0.1231603669339886 No Hit TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG 3214 0.1203885095273234 No Hit GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA 3193 0.11960190134435085 No Hit GGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTG 3179 0.11907749588903581 No Hit TTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCTCCCA 3055 0.11443276185624549 No Hit CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA 3020 0.1131217482179579 No Hit GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG 2877 0.10776532106724002 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2822 0.10570515677850237 No Hit GCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTT 2769 0.10371990755480974 No Hit GCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTA 2704 0.10128516794084706 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2695 0.10094805014814455 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1237259756750783E-4 2 0.0 0.0 0.0 0.0 1.1237259756750783E-4 3 0.0 0.0 0.0 0.0 1.1237259756750783E-4 4 0.0 0.0 0.0 0.0 1.1237259756750783E-4 5 0.0 0.0 0.0 0.0 1.4983013009001045E-4 6 0.0 0.0 0.0 0.0 2.2474519513501567E-4 7 0.0 0.0 0.0 0.0 2.2474519513501567E-4 8 0.0 0.0 0.0 0.0 2.2474519513501567E-4 9 0.0 0.0 0.0 0.0 2.2474519513501567E-4 10-11 0.0 0.0 0.0 5.618629878375392E-5 2.622027276575183E-4 12-13 7.491506504500523E-5 0.0 0.0 1.1237259756750783E-4 5.431342215762879E-4 14-15 1.1237259756750783E-4 0.0 0.0 1.1237259756750783E-4 7.304218841888009E-4 16-17 1.3110136382875914E-4 0.0 0.0 1.1237259756750783E-4 8.427944817563087E-4 18-19 1.4983013009001045E-4 0.0 0.0 1.4983013009001045E-4 0.0010300821443688219 20-21 1.4983013009001045E-4 0.0 0.0 2.0601642887376436E-4 0.0012173698069813349 22-23 1.4983013009001045E-4 0.0 0.0 5.056766890537852E-4 0.0012360985732425861 24-25 2.622027276575183E-4 0.0 0.0 8.802520142788114E-4 0.0012360985732425861 26-27 2.809314939187696E-4 0.0 0.0 0.0018541478598638793 0.0012548273395038374 28-29 3.745753252250261E-4 0.0 0.0 0.0060306627361229204 0.0013110136382875914 30-31 5.805917540987904E-4 0.0 0.0 0.018091988208368763 0.0013110136382875914 32-33 5.993205203600418E-4 0.0 0.0 0.043319636362274275 0.0013110136382875914 34-35 6.929643516662984E-4 0.0 0.0 0.0787357333623005 0.0014233862358550993 36-37 7.491506504500523E-4 0.0 0.0 0.13726312792871082 0.0014233862358550993 38-39 7.866081829725549E-4 0.0 0.0 0.2227412171450618 0.0014795725346388533 40-41 7.866081829725549E-4 0.0 0.0 0.3114781116908705 0.0014983013009001046 42-43 8.427944817563087E-4 0.0 0.0 0.40412931838528066 0.0015732163659451098 44-45 8.6152324801756E-4 0.0 0.0 0.5156965790035547 0.0016106738984676123 46-47 9.177095468013139E-4 0.0 0.0 0.648446074263304 0.0016668601972513663 48-49 9.364383130625653E-4 0.0 0.0 0.7789668463379644 0.0017605040285576228 50-51 9.364383130625653E-4 0.0 0.0 0.9251074094745082 0.0019477916911701358 52-53 9.364383130625653E-4 0.0 0.0 1.0628387565597504 0.0020976218212601463 54-55 9.364383130625653E-4 0.0 0.0 1.2061887335233679 0.0021163505875213973 56-57 9.364383130625653E-4 0.0 0.0 1.3723690765594507 0.002191265652566403 58-59 9.364383130625653E-4 0.0 0.0 1.5375942525162096 0.0023036382501339103 60-61 9.364383130625653E-4 0.0 0.0 1.7204057399922839 0.0023410957826564133 62-63 9.364383130625653E-4 0.0 0.0 1.896156482587866 0.0025471122115301777 64-65 9.364383130625653E-4 0.0 0.0 2.0757091647344823 0.002565840977791429 66-67 9.364383130625653E-4 0.0 0.0 2.2887114234236936 0.0026220272765751828 68-69 9.738958455850679E-4 0.0 0.0 2.517239829343482 0.0026594848090976857 70-71 9.926246118463194E-4 0.0 0.0 2.7476411118893953 0.002753128640403942 72-73 0.0010113533781075706 0.0 0.0 2.9928193910154364 0.0028655012379714497 74-75 0.0010300821443688219 0.0 0.0 3.2380913139727836 0.0029778738355389577 76-77 0.0010488109106300731 0.0 0.0 3.4944881240893135 0.002996602601800209 78-79 0.0010488109106300731 0.0 0.0 3.7878180612730317 0.002996602601800209 80-81 0.0010862684431525757 0.0 0.0 4.087684337881926 0.0030153313680614602 82-83 0.0010862684431525757 0.0 0.0 4.400005244054553 0.0032213477969352247 84-85 0.0010862684431525757 0.0 0.0 4.723919256542894 0.003240076563196476 86-87 0.0010862684431525757 0.0 0.0 5.052140885271323 0.003371177927025235 88-89 0.0010862684431525757 0.0 0.0 5.4003273788342465 0.003483550524592743 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTAAG 2195 0.0 37.227196 2 TAAGGTG 2515 0.0 34.74885 5 AAGGTAA 2465 0.0 32.95684 1 GGTATCA 11855 0.0 32.54025 1 GTAAGGT 2825 0.0 30.599447 4 GGTAAGG 2845 0.0 30.221354 3 GTATCAA 17045 0.0 29.628021 1 GTGTAAG 985 0.0 26.527288 1 AAGGTGT 3595 0.0 25.366167 6 GGTGTGC 3610 0.0 24.866068 8 TATCAAC 20320 0.0 24.712557 2 GTGTGCA 3825 0.0 24.585907 9 ATCAACG 20530 0.0 24.41075 3 TCAACGC 20710 0.0 24.310081 4 AGGTGTG 3905 0.0 24.082226 7 CAACGCA 20890 0.0 24.077873 5 AACGCAG 21315 0.0 23.619625 6 GAGTACT 13755 0.0 23.10032 12-13 TAAGGTA 1220 0.0 22.190937 4 GTACTTT 14605 0.0 21.60956 14-15 >>END_MODULE