FastQCFastQC Report
Thu 26 May 2016
SRR937969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937969_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2692389
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153730.5709798992641851No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123200.4575861808973369No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81810.30385653781827215No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA72820.2704661176375331No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA72750.27020612548929596No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA71390.2651548494664033No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC66560.24721539123804176No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA64510.2396013354682403No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG56800.21096505742669427No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50130.18619151987324267No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA49560.18407444095188324No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA48440.17991456658008928No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA46930.1743061645252599No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT45100.16750922693563225No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG41250.1532096587825905No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA40650.1509811546548437No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA36730.1364215943535648No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT36610.13597589352801545No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG36250.1346387910513674No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG36070.13397023981304337No Hit
TCTTAGGAGTGGGGGTGGCTTTTGGGAGGGTGAGGGACTTCCTGTAACCA35470.1317417356852966No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT35400.13148174353705946No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT34240.12717330222341572No Hit
CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT34230.12713616048795326No Hit
TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG33070.1228277191743095No Hit
GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA32320.12004208901462605No Hit
GGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTG31580.11729360059040503No Hit
CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA31130.11562222249459495No Hit
TTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCTCCCA30220.11224232456751235No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30060.11164805680011321No Hit
GCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTT28520.1059282295388965No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28520.1059282295388965No Hit
GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG27950.10381115061753707No Hit
GTTACAGGAAGTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGG27210.10106266219331606No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA169100.030.5090221
AGGTAAG22300.029.3979032
GGTATCA116850.029.352871
TAAGGTG25100.028.3848785
GTGTAAG11050.028.3742081
AAGGTAA23950.026.777521
GTAAGGT27650.025.7671054
TAAGGTA12550.025.3571594
TATCAAC202750.025.281492
ATCAACG205900.024.8231953
TCAACGC207100.024.7004644
CAACGCA208500.024.4890485
TGTAAGG12850.024.3996122
GGTAAGG28850.024.038583
AACGCAG213000.023.9939736
GAGTACT135550.022.46094512-13
AGAGTAC175450.022.32064810-11
ACGCAGA236100.021.5458187
GGTGTGC35450.021.4374548
AGTACTT140300.021.39581912-13
Produced by FastQC (version 0.11.5)