##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937969_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2692389 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.551286236869934 34.0 31.0 34.0 31.0 34.0 2 32.75799819416882 34.0 31.0 34.0 31.0 34.0 3 32.812604345063065 34.0 31.0 34.0 31.0 34.0 4 36.2636610088661 37.0 37.0 37.0 35.0 37.0 5 36.229625436740385 37.0 37.0 37.0 35.0 37.0 6 36.271190010061694 37.0 37.0 37.0 35.0 37.0 7 36.239435311910725 37.0 37.0 37.0 35.0 37.0 8 36.22781106296304 37.0 37.0 37.0 35.0 37.0 9 37.948587666938174 39.0 38.0 39.0 35.0 39.0 10-11 37.93244326878471 39.0 38.0 39.0 35.0 39.0 12-13 37.88979007119699 39.0 38.0 39.0 35.0 39.0 14-15 39.217531530547774 40.5 39.0 41.0 36.0 41.0 16-17 39.17970137301853 40.0 39.0 41.0 36.0 41.0 18-19 39.22465438686609 40.0 39.0 41.0 36.0 41.0 20-21 39.15755412757963 40.0 39.0 41.0 36.0 41.0 22-23 39.027398343998584 40.0 38.5 41.0 35.0 41.0 24-25 38.96645822724725 40.0 38.5 41.0 35.0 41.0 26-27 38.908861980939605 40.0 38.0 41.0 35.0 41.0 28-29 38.85146648571213 40.0 38.0 41.0 35.0 41.0 30-31 38.6246662350797 40.0 38.0 41.0 35.0 41.0 32-33 38.40645426793825 40.0 38.0 41.0 34.0 41.0 34-35 38.1838311625846 40.0 38.0 41.0 34.0 41.0 36-37 38.022740213245555 40.0 38.0 41.0 33.5 41.0 38-39 37.953327695217894 40.0 38.0 41.0 33.0 41.0 40-41 37.86978441822485 40.0 38.0 41.0 33.0 41.0 42-43 37.686919126470954 40.0 37.0 41.0 33.0 41.0 44-45 37.46435043375976 40.0 37.0 41.0 33.0 41.0 46-47 37.112705482008735 40.0 37.0 41.0 31.5 41.0 48-49 36.71030672016562 40.0 36.0 41.0 31.0 41.0 50-51 36.377381017378994 40.0 35.5 41.0 30.0 41.0 52-53 35.94935947220108 39.0 35.0 41.0 29.0 41.0 54-55 35.70028160863828 39.0 35.0 41.0 28.5 41.0 56-57 35.426203828644375 39.0 35.0 40.0 28.0 41.0 58-59 35.06492208221026 38.0 35.0 40.0 27.5 41.0 60-61 34.68463732395281 38.0 34.0 40.0 26.0 41.0 62-63 34.32400370080252 37.0 34.0 40.0 26.0 41.0 64-65 33.92532394093127 36.5 34.0 39.5 25.5 41.0 66-67 33.50209553671479 36.0 33.5 39.0 24.5 41.0 68-69 33.04641268405123 35.5 33.0 39.0 23.0 40.0 70-71 32.512934609374796 35.0 32.5 38.0 21.5 40.0 72-73 32.11331349221825 35.0 32.0 37.0 20.5 39.5 74-75 31.705494822627784 35.0 32.0 37.0 19.5 39.0 76-77 30.40871025695024 33.5 30.0 35.5 18.5 37.5 78-79 31.11165808506869 34.5 31.5 36.0 18.5 38.0 80-81 30.966786188771387 35.0 32.0 35.5 18.0 37.0 82-83 30.612864262927832 35.0 31.0 35.0 14.0 37.0 84-85 30.271257050894206 34.0 31.0 35.0 8.5 36.0 86-87 29.90157904374145 34.0 31.0 35.0 6.5 36.0 88-89 29.457092010107008 34.0 30.0 35.0 2.0 35.5 90-91 29.149436244168285 34.0 30.0 35.0 2.0 35.0 92-93 28.797920359947987 34.0 29.0 35.0 2.0 35.0 94-95 28.42330584473492 34.0 29.0 35.0 2.0 35.0 96-97 27.900977161918284 34.0 29.0 35.0 2.0 35.0 98-99 27.2666004058106 33.5 27.0 35.0 2.0 35.0 100-101 24.828277600302187 31.0 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2204301328895113 1101 2 0.18866056526074004 1101 3 0.14056615397393557 1101 4 -0.012427215957750093 1101 5 0.061653742995837035 1101 6 0.023321393555910674 1101 7 0.10417819737840972 1101 8 0.11034397173333588 1101 9 0.07644359278196333 1101 10-11 0.09683686359573329 1101 12-13 0.15313718981133917 1101 14-15 0.13271145463865963 1101 16-17 0.19970609226107428 1101 18-19 0.17953975943081701 1101 20-21 0.19062882876157516 1101 22-23 0.2222874167964619 1101 24-25 0.13508077217090175 1101 26-27 0.1753688322569289 1101 28-29 0.17492670844301728 1101 30-31 0.21837374671324739 1101 32-33 0.15992107926548726 1101 34-35 0.14435247737975487 1101 36-37 0.23859854007743309 1101 38-39 0.3399815800344257 1101 40-41 0.2836289633451088 1101 42-43 0.3383487756994299 1101 44-45 0.31931388810010475 1101 46-47 0.3559891945315954 1101 48-49 0.4029109063341707 1101 50-51 0.34133467079271895 1101 52-53 0.22840131186286072 1101 54-55 0.1535762656504076 1101 56-57 0.3717808870075032 1101 58-59 0.34632471462875003 1101 60-61 0.19928425148058437 1101 62-63 0.11884488330808551 1101 64-65 0.2978040481131856 1101 66-67 0.20847442495036717 1101 68-69 0.17299367671949284 1101 70-71 0.24419106098542187 1101 72-73 0.33748521921611285 1101 74-75 0.25382107368506013 1101 76-77 0.23185635269552307 1101 78-79 0.3648888623118687 1101 80-81 0.43779778494880617 1101 82-83 0.40760058383595776 1101 84-85 0.40905139824879555 1101 86-87 0.40837888205294703 1101 88-89 0.37815229305799747 1101 90-91 0.41188750371057026 1101 92-93 0.4982512222955151 1101 94-95 0.5389923647381494 1101 96-97 0.6500488896923571 1101 98-99 0.6441779867648911 1101 100-101 0.8015338331770003 1102 1 0.08375845798254744 1102 2 0.10920250344973681 1102 3 0.1449381730518411 1102 4 0.06924175100533603 1102 5 0.09797513225840504 1102 6 0.0724670809368888 1102 7 0.0975550907553071 1102 8 0.10414976848030477 1102 9 0.13376377657273508 1102 10-11 0.20342502900743398 1102 12-13 0.17095021938906996 1102 14-15 0.26140998186659914 1102 16-17 0.22398455624541924 1102 18-19 0.205661372317131 1102 20-21 0.16122448906311604 1102 22-23 0.21174958419980783 1102 24-25 0.13090136652090933 1102 26-27 0.10463034975374086 1102 28-29 0.20560945971400457 1102 30-31 0.22670678886981932 1102 32-33 0.22600277181776107 1102 34-35 0.23861556723107924 1102 36-37 0.14514827015657517 1102 38-39 0.1324670357551767 1102 40-41 0.29099063982442885 1102 42-43 0.2733100783077944 1102 44-45 0.26186573212253705 1102 46-47 0.21844660993018294 1102 48-49 0.3984825445528699 1102 50-51 0.3807426793183737 1102 52-53 0.29913586347976917 1102 54-55 0.42573729222319656 1102 56-57 0.3623253584343331 1102 58-59 0.36568476634173663 1102 60-61 0.3834122061967733 1102 62-63 0.2881123408108337 1102 64-65 0.39059873355492414 1102 66-67 0.2374564127558827 1102 68-69 0.45439097576385024 1102 70-71 0.532782755459472 1102 72-73 0.40229723549871466 1102 74-75 0.375689904377424 1102 76-77 0.436392595467062 1102 78-79 0.4595100995801644 1102 80-81 0.5113691261672066 1102 82-83 0.5762308009955852 1102 84-85 0.5715642828204999 1102 86-87 0.4085243248454482 1102 88-89 0.3187535439533633 1102 90-91 0.4897339021452005 1102 92-93 0.5477476320860433 1102 94-95 0.5200297693049691 1102 96-97 0.44290341195864613 1102 98-99 0.45099632740675943 1102 100-101 0.8967019004038903 1103 1 0.10752380294772479 1103 2 0.160344269143593 1103 3 0.10197319753720535 1103 4 0.0658379855423874 1103 5 0.09261776216654027 1103 6 0.09743566837001083 1103 7 0.11630976261091064 1103 8 0.07144581011765183 1103 9 0.048480701099144596 1103 10-11 0.11952798301066991 1103 12-13 0.1362034118412936 1103 14-15 0.18764029686870032 1103 16-17 0.12889789464072265 1103 18-19 0.1377246912876231 1103 20-21 0.2147161085863516 1103 22-23 0.14433227446389196 1103 24-25 0.1447609028368717 1103 26-27 0.18402766668652504 1103 28-29 0.18659927369935758 1103 30-31 0.161658125726305 1103 32-33 0.21729607628496694 1103 34-35 0.21463440044488635 1103 36-37 0.19878391134795237 1103 38-39 0.19503134619139928 1103 40-41 0.3459770845960435 1103 42-43 0.261358555209128 1103 44-45 0.2949253203503517 1103 46-47 0.17266170869331887 1103 48-49 0.14441770362139295 1103 50-51 0.17000973627899185 1103 52-53 0.14926298851569442 1103 54-55 0.13892871631855286 1103 56-57 0.07701919230125043 1103 58-59 0.19986829663390893 1103 60-61 0.13315555435924864 1103 62-63 0.19324579892361982 1103 64-65 0.2215883368590852 1103 66-67 0.20232195180059165 1103 68-69 0.26796187883616795 1103 70-71 0.26754709168307755 1103 72-73 0.13209311912516597 1103 74-75 0.012973482114993828 1103 76-77 0.13519061708632307 1103 78-79 0.15693709222401964 1103 80-81 0.1945906439651317 1103 82-83 0.2762563355569938 1103 84-85 0.32932514617520425 1103 86-87 0.4093506165108174 1103 88-89 0.3228008983452675 1103 90-91 0.3126040205564635 1103 92-93 0.35842765200805715 1103 94-95 0.31772542261472836 1103 96-97 0.2515473244939237 1103 98-99 0.29603263620471765 1103 100-101 0.33626412172533193 1104 1 0.13932327516982923 1104 2 0.13236132169436132 1104 3 0.13346328162374732 1104 4 0.018979199962750215 1104 5 0.04048277851020288 1104 6 0.0067683448793403045 1104 7 0.016926135867379344 1104 8 0.06452402128825696 1104 9 0.06145650590352858 1104 10-11 0.10912071629438458 1104 12-13 0.13608760866842573 1104 14-15 0.1858420672581218 1104 16-17 0.08566094221978204 1104 18-19 0.10481801425848403 1104 20-21 0.18640092855312673 1104 22-23 0.23696445765961016 1104 24-25 0.15069745566177062 1104 26-27 0.2202878138901454 1104 28-29 0.21650597665576043 1104 30-31 0.18010600591958337 1104 32-33 0.28201890435495613 1104 34-35 0.2948018146809801 1104 36-37 0.34610847072676876 1104 38-39 0.3031588634282869 1104 40-41 0.3055507897979908 1104 42-43 0.26212530659260125 1104 44-45 0.28639722689583635 1104 46-47 0.3712178288870476 1104 48-49 0.416028868027432 1104 50-51 0.5769794747361345 1104 52-53 0.5616221196867741 1104 54-55 0.47075563349739724 1104 56-57 0.4684460897500742 1104 58-59 0.5209769780302125 1104 60-61 0.49957758254155493 1104 62-63 0.5602760111414327 1104 64-65 0.5668836768445544 1104 66-67 0.6703794053512624 1104 68-69 0.741861582696238 1104 70-71 0.7951910085463538 1104 72-73 0.7443668642298107 1104 74-75 0.7431720090670169 1104 76-77 0.6295611600474835 1104 78-79 0.6641445552286633 1104 80-81 0.7888475496284677 1104 82-83 0.7016215463551312 1104 84-85 0.7600139829706727 1104 86-87 0.7211068281455795 1104 88-89 0.6666291346526698 1104 90-91 0.6547593950568107 1104 92-93 0.7378089642690604 1104 94-95 0.6767563626930269 1104 96-97 0.7715072858433274 1104 98-99 0.7231990803817432 1104 100-101 0.9362104807471034 1105 1 0.11458589149754772 1105 2 0.08332432102060494 1105 3 0.034987909238196835 1105 4 0.05186163508098218 1105 5 0.07827978871087993 1105 6 0.0948617902296931 1105 7 0.0012346683430592975 1105 8 0.0344168259590063 1105 9 0.04072366091224211 1105 10-11 0.02896850684654595 1105 12-13 0.021241116712445773 1105 14-15 0.14414060636654114 1105 16-17 0.11406999011584418 1105 18-19 0.06449565668333435 1105 20-21 0.13753418664941108 1105 22-23 0.07396785380889526 1105 24-25 0.09887487883931811 1105 26-27 0.08339139178466581 1105 28-29 -0.0844788454918799 1105 30-31 0.12332707182828528 1105 32-33 0.08814824919279829 1105 34-35 0.1715210715811537 1105 36-37 0.10116484319443231 1105 38-39 -0.0390952578675865 1105 40-41 0.00820895211552397 1105 42-43 -0.020165626702066675 1105 44-45 -0.01762051612070792 1105 46-47 0.049349629411032936 1105 48-49 0.09583494044949958 1105 50-51 0.05225817793353826 1105 52-53 0.10414393814070166 1105 54-55 0.19928934371478846 1105 56-57 0.11644048656961559 1105 58-59 0.033965123535871555 1105 60-61 -0.04376925211570892 1105 62-63 -2.9586972750905716E-5 1105 64-65 0.025615833521641207 1105 66-67 0.09001682914608722 1105 68-69 0.20766169846346116 1105 70-71 0.24999687715287422 1105 72-73 0.08719028601326784 1105 74-75 0.03550478860440265 1105 76-77 0.11039296330841353 1105 78-79 0.1832114993173981 1105 80-81 0.23462919388416026 1105 82-83 0.2833847720913276 1105 84-85 0.3664415099485687 1105 86-87 0.3951439016106022 1105 88-89 0.18335059858401337 1105 90-91 0.09712014099840616 1105 92-93 0.15234037195314087 1105 94-95 0.14173357320362 1105 96-97 0.17818211386668636 1105 98-99 0.3057089261283146 1105 100-101 0.26915430801867046 1106 1 -0.1673098616808133 1106 2 0.008792013783050834 1106 3 0.003002623678270311 1106 4 0.008875715432687059 1106 5 -0.01958548447576902 1106 6 -0.08600848415024842 1106 7 0.009244205750206902 1106 8 -0.12720630006584344 1106 9 -0.08054931707920332 1106 10-11 0.014367437057281052 1106 12-13 -0.06144620928834854 1106 14-15 -0.13266603369701357 1106 16-17 -0.12469064614879244 1106 18-19 -0.04226431155895938 1106 20-21 0.09052639109228977 1106 22-23 0.056173766227296085 1106 24-25 0.002315712728638175 1106 26-27 -0.07956048732965115 1106 28-29 0.062182136534609356 1106 30-31 0.11931367941059534 1106 32-33 -0.08069853447032216 1106 34-35 0.03739034617114356 1106 36-37 0.12392675100117145 1106 38-39 0.027225170182738623 1106 40-41 0.11492517078374931 1106 42-43 0.12140820533154084 1106 44-45 0.08323704564261192 1106 46-47 0.09598534325404273 1106 48-49 0.14589574113961845 1106 50-51 0.14798238622751114 1106 52-53 0.13891040656009324 1106 54-55 -0.053699018618068806 1106 56-57 0.11203131475186012 1106 58-59 0.18365274877253768 1106 60-61 0.26920040727421934 1106 62-63 0.17674694721403483 1106 64-65 -0.10673714477268703 1106 66-67 -0.04393167648344587 1106 68-69 0.010002875590622295 1106 70-71 -0.12316020709506148 1106 72-73 -0.022317071346996897 1106 74-75 -0.06677757899915093 1106 76-77 -0.014491878557908677 1106 78-79 -0.13564352945776292 1106 80-81 -0.2093724730867912 1106 82-83 -0.3393160265347106 1106 84-85 -0.029267708507358492 1106 86-87 0.12510526456688353 1106 88-89 0.021460698313408244 1106 90-91 0.08461035693901309 1106 92-93 0.08561350755522668 1106 94-95 -0.07602824906710026 1106 96-97 -0.016234122385501593 1106 98-99 0.0726643399838629 1106 100-101 0.10665682151583766 1107 1 0.04958341294487667 1107 2 0.07428028705787426 1107 3 0.009574694557372254 1107 4 0.06917532921367098 1107 5 0.050411077665593496 1107 6 0.015005884178570739 1107 7 0.02854048755539651 1107 8 0.035256790961128104 1107 9 -0.013728173475122674 1107 10-11 -0.013279387377494345 1107 12-13 0.04734701431621602 1107 14-15 0.11541960712223442 1107 16-17 0.08815907728894956 1107 18-19 0.02577935217951932 1107 20-21 0.09864416482144378 1107 22-23 0.09072044495980691 1107 24-25 0.0508281254252978 1107 26-27 0.005896570508781451 1107 28-29 0.04442775699875057 1107 30-31 0.1488515928037657 1107 32-33 0.08111905792446805 1107 34-35 -0.012827593796750136 1107 36-37 0.12954874337494005 1107 38-39 0.17327695356190276 1107 40-41 0.15625248559745586 1107 42-43 0.1424709570533338 1107 44-45 0.1950874030438925 1107 46-47 0.2570088143070848 1107 48-49 0.2524651582798967 1107 50-51 0.1953314878368957 1107 52-53 0.20582343087021115 1107 54-55 0.2423324816618404 1107 56-57 0.23917714263436096 1107 58-59 0.19547095459438424 1107 60-61 0.11531149304813226 1107 62-63 0.12054255918354784 1107 64-65 0.13836950752378385 1107 66-67 0.08889521946625933 1107 68-69 0.28354874006537756 1107 70-71 0.17088672327590615 1107 72-73 -0.014300979821548765 1107 74-75 0.10549041021626593 1107 76-77 0.12190282922010454 1107 78-79 0.16498827374843472 1107 80-81 0.2415446937229646 1107 82-83 0.22238693501114426 1107 84-85 0.37466167856965527 1107 86-87 0.19265091887619334 1107 88-89 0.2766230871264206 1107 90-91 0.06494248502151478 1107 92-93 0.215006412124378 1107 94-95 0.2823945179316958 1107 96-97 0.5488094162672041 1107 98-99 0.6209969252028849 1107 100-101 0.5471811336307262 1108 1 0.037859259712654136 1108 2 0.014306513295125 1108 3 0.030766458233571825 1108 4 -0.0010881113503913298 1108 5 -0.0010533225890156928 1108 6 2.8881845690165164E-4 1108 7 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-0.3533409548249473 2316 100-101 -0.3898007990562178 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 7.0 4 19.0 5 38.0 6 97.0 7 243.0 8 641.0 9 1244.0 10 2166.0 11 3517.0 12 5554.0 13 7765.0 14 10745.0 15 14627.0 16 18843.0 17 27456.0 18 27143.0 19 20155.0 20 16337.0 21 14937.0 22 14964.0 23 16036.0 24 18061.0 25 20471.0 26 23649.0 27 28409.0 28 33878.0 29 41315.0 30 50639.0 31 62355.0 32 78449.0 33 102999.0 34 148264.0 35 232000.0 36 400636.0 37 644214.0 38 542500.0 39 62005.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55232471979346 18.516120813151442 14.939148837705101 22.992405629349992 2 15.782247525421866 20.223152229553477 39.00175113132593 24.99284911369872 3 19.19508323926455 23.581010029422263 30.493254720432777 26.730652010880405 4 13.035560611783808 16.790850059185356 36.53116247317902 33.64242685585181 5 13.3263432587193 37.344975038896685 34.31082952723399 15.017852175150026 6 32.38491911829977 36.53409667028056 16.305704710574883 14.77527950084479 7 28.45068078944016 33.739886769705265 19.9336351470757 17.87579729377887 8 27.46480808801135 33.28111187870954 19.51106455998039 19.743015473298716 9 26.64292068184899 16.422874143185318 20.049398581555014 36.88480659341067 10-11 25.086066686500352 26.221489539587335 26.73343265033396 21.959011123578353 12-13 26.743145214157387 23.658821217884935 27.1630696752958 22.43496389266187 14-15 22.67865081903098 25.153813211983856 25.98777516918989 26.179760799795275 16-17 21.789581942836513 28.306893930024074 27.36083610689861 22.542688020240806 18-19 22.035430244292336 26.18046649276906 30.091231244816406 21.692872018122195 20-21 22.031307511656006 25.257457224791814 30.755288333149483 21.9559469304027 22-23 22.04504995377711 25.229489497988588 30.94933533007303 21.77612521816127 24-25 22.17395826175455 25.553095824875427 30.57225262837287 21.70069328499715 26-27 21.17258761334945 25.896082663113706 30.54572492474018 22.38560479879666 28-29 21.58202651660412 26.83652452088545 30.058514958001048 21.522934004509377 30-31 21.49873216685999 25.90246060283265 29.804738468326832 22.794068761980533 32-33 21.05495196904893 26.035752402568956 30.03786428988346 22.871431338498656 34-35 22.645336774764914 26.145035686649926 29.444764540178454 21.764862998406702 36-37 22.690181333433372 25.21654767633168 29.76356137889998 22.32970961133497 38-39 21.74673366684832 25.865558318130503 29.655790095815902 22.73191791920527 40-41 22.354365005439274 25.733456835273312 29.74746800282541 22.164710156462 42-43 22.044991590495773 25.895924213429055 29.192940176629307 22.866144019445862 44-45 21.943239266966508 26.035882077866702 29.892315880844727 22.12856277432206 46-47 22.945648445608796 26.75737546791631 28.501235984331387 21.795740102143505 48-49 22.28905258489765 26.802794841310078 28.241832810934824 22.666319762857448 50-51 22.392046617335012 26.986070734949518 28.704693118267826 21.91718952944764 52-53 23.24927787180827 26.320007992901473 28.421320247557098 22.00939388773316 54-55 21.926976376741994 26.853177605464886 28.788132026984215 22.43171399080891 56-57 22.435502447826075 26.943599160448212 28.264619265641034 22.356279126084676 58-59 22.420311478021933 26.33076052531785 28.1396373258099 23.10929067085031 60-61 21.458619092560546 26.92983814745937 28.9361047010666 22.67543805891348 62-63 21.843444613686952 26.975949612036004 29.018615066396425 22.161990707880623 64-65 21.213929339333955 27.270112156898573 28.585969560862118 22.929988942905354 66-67 21.215489292223374 27.000871716531304 28.668219934043705 23.115419057201617 68-69 22.2580599632593 26.3883616812176 28.817400354035943 22.53617800148716 70-71 21.998009945813923 26.931565238158377 27.871566850109698 23.198857965918002 72-73 21.813731225316996 26.961278626528333 28.283041566430406 22.94194858172426 74-75 21.80420437017088 27.758748828642517 27.87843807117025 22.558608730016353 76-77 22.024584857537302 26.824597040026532 28.333907173146226 22.816910929289936 78-79 22.144255529197306 27.298117025437264 28.085596100712046 22.47203134465339 80-81 22.692504686358472 27.328536106781005 27.5823255851959 22.396633621664623 82-83 22.310551508651706 27.367141616873496 27.895687377838442 22.42661949663635 84-85 21.98902911757698 27.807325943516503 27.847830217079505 22.35581472182701 86-87 22.588704941834013 27.813225542744508 27.058354668228606 22.539714847192872 88-89 22.053113805827056 28.040641237325147 26.799512774623725 23.106732182224075 90-91 21.93552436755341 27.811196147351332 27.09504578010211 23.158233704993144 92-93 22.401988722623447 27.204651346772362 27.554193192903753 22.839166737700435 94-95 21.824650895542955 26.738279646811808 28.01885240208603 23.418217055559207 96-97 22.12159907056521 26.172778153528338 28.14429861361044 23.561324162296014 98-99 21.787657727022356 27.07067960833297 27.211409644000177 23.93025302064449 100-101 21.6866879191677 26.42251918277782 27.950474467099667 23.940318430954814 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1202.0 1 830.0 2 687.5 3 925.5 4 768.0 5 686.0 6 1421.0 7 3182.5 8 5161.5 9 5884.0 10 4778.5 11 3494.5 12 3223.5 13 3365.0 14 3560.5 15 3754.5 16 3905.0 17 4111.0 18 4473.0 19 4984.0 20 5653.5 21 6702.0 22 8027.0 23 9848.5 24 12207.5 25 15257.0 26 19013.0 27 23163.0 28 27009.5 29 30464.5 30 34286.5 31 41238.0 32 54966.0 33 57405.0 34 49336.0 35 49015.5 36 53088.0 37 57725.5 38 61063.5 39 63522.0 40 67552.0 41 74462.0 42 78353.5 43 79303.5 44 80998.0 45 88693.0 46 92470.5 47 97055.5 48 105082.0 49 99683.5 50 92477.0 51 89336.0 52 87861.0 53 92456.5 54 99457.5 55 95546.5 56 86597.0 57 80222.0 58 76250.5 59 66866.0 60 49908.0 61 41066.0 62 37649.0 63 27332.0 64 16572.5 65 12220.5 66 9269.5 67 7340.0 68 5857.0 69 4464.5 70 3423.5 71 2305.5 72 1647.5 73 1289.5 74 870.0 75 596.0 76 430.5 77 264.5 78 162.0 79 85.0 80 43.5 81 30.0 82 20.0 83 10.5 84 7.5 85 5.5 86 3.5 87 2.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015413820216915163 3 0.0077997644471136975 4 0.0 5 0.0 6 0.0 7 0.0 8 3.342756191620156E-4 9 1.485669418497847E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.2285041277467706E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.004976992551967788 26-27 0.020335100165689284 28-29 1.8570867731223088E-5 30-31 0.0 32-33 3.899882223556849E-4 34-35 0.01805088343474884 36-37 0.003101334911114256 38-39 0.016936631370875456 40-41 0.014615272904472572 42-43 0.007316921886101896 44-45 0.002599921482371232 46-47 1.8570867731223088E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.599921482371232E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.5712603193669266E-5 84-85 0.0029899097047269172 86-87 2.971338836995694E-4 88-89 1.8570867731223088E-5 90-91 2.7856301596834634E-4 92-93 2.7856301596834634E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2692389.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.29460206843925 #Duplication Level Percentage of deduplicated Percentage of total 1 67.05046923148426 26.347215069533032 2 16.009425693059377 12.581680239060308 3 6.442274392643179 7.594398260238293 4 3.352417717503987 5.269276807060182 5 1.9271685878756981 3.7863661389685768 6 1.1866510832154684 2.7977389265420527 7 0.7936141305341781 2.182932601866165 8 0.5596794355661708 1.7593904565169096 9 0.42539933134044033 1.5044307700682467 >10 2.0178475714217603 14.167091158208066 >50 0.13969012705086442 3.799519756174196 >100 0.07740130563323844 5.879807987576002 >500 0.0089806963356782 2.455948773003933 >1k 0.008035359879289373 6.851582595994676 >5k 7.562691651095878E-4 1.993919115004344 >10k+ 1.8906729127739696E-4 1.028701344184945 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15373 0.5709798992641851 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12320 0.4575861808973369 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8181 0.30385653781827215 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 7282 0.2704661176375331 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 7275 0.27020612548929596 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 7139 0.2651548494664033 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 6656 0.24721539123804176 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 6451 0.2396013354682403 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5680 0.21096505742669427 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5013 0.18619151987324267 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4956 0.18407444095188324 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4844 0.17991456658008928 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4693 0.1743061645252599 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 4510 0.16750922693563225 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4125 0.1532096587825905 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 4065 0.1509811546548437 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3673 0.1364215943535648 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 3661 0.13597589352801545 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3625 0.1346387910513674 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3607 0.13397023981304337 No Hit TCTTAGGAGTGGGGGTGGCTTTTGGGAGGGTGAGGGACTTCCTGTAACCA 3547 0.1317417356852966 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3540 0.13148174353705946 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3424 0.12717330222341572 No Hit CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT 3423 0.12713616048795326 No Hit TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG 3307 0.1228277191743095 No Hit GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA 3232 0.12004208901462605 No Hit GGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTG 3158 0.11729360059040503 No Hit CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA 3113 0.11562222249459495 No Hit TTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCTCCCA 3022 0.11224232456751235 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3006 0.11164805680011321 No Hit GCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTT 2852 0.1059282295388965 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2852 0.1059282295388965 No Hit GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG 2795 0.10381115061753707 No Hit GTTACAGGAAGTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGG 2721 0.10106266219331606 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.428347092489235E-5 2 3.7141735462446175E-5 0.0 0.0 0.0 7.428347092489235E-5 3 3.7141735462446175E-5 0.0 0.0 0.0 7.428347092489235E-5 4 3.7141735462446175E-5 0.0 0.0 0.0 7.428347092489235E-5 5 3.7141735462446175E-5 0.0 0.0 0.0 7.428347092489235E-5 6 7.428347092489235E-5 0.0 0.0 0.0 7.428347092489235E-5 7 1.1142520638733853E-4 0.0 0.0 0.0 7.428347092489235E-5 8 1.1142520638733853E-4 0.0 0.0 0.0 7.428347092489235E-5 9 1.1142520638733853E-4 0.0 0.0 0.0 7.428347092489235E-5 10-11 1.1142520638733853E-4 0.0 0.0 0.0 7.428347092489235E-5 12-13 1.1142520638733853E-4 0.0 0.0 3.7141735462446175E-5 3.157047514307925E-4 14-15 1.485669418497847E-4 0.0 0.0 3.7141735462446175E-5 6.128386351303619E-4 16-17 1.485669418497847E-4 0.0 0.0 3.7141735462446175E-5 8.35689047905039E-4 18-19 1.485669418497847E-4 0.0 0.0 3.7141735462446175E-5 8.728307833674851E-4 20-21 1.485669418497847E-4 0.0 0.0 3.7141735462446175E-5 0.0011328229316046085 22-23 1.8570867731223088E-4 0.0 0.0 2.0427954504345397E-4 0.001299960741185616 24-25 3.342756191620156E-4 0.0 0.0 4.0855909008690795E-4 0.001299960741185616 26-27 3.528464868932387E-4 0.0 0.0 0.0017828033021974167 0.0014113859475729548 28-29 4.828425610118003E-4 0.0 0.0 0.005608402054829373 0.001485669418497847 30-31 5.199842964742464E-4 0.0 0.0 0.015673812365152286 0.001485669418497847 32-33 5.199842964742464E-4 0.0 0.0 0.04148731851155238 0.0015228111539602933 34-35 5.199842964742464E-4 0.0 0.0 0.0782576366193741 0.0015228111539602933 36-37 5.942677673991388E-4 0.0 0.0 0.13816725592029977 0.0015228111539602933 38-39 7.056929737864774E-4 0.0 0.0 0.2250603460346926 0.0016156654926164087 40-41 7.056929737864774E-4 0.0 0.0 0.31631759006592286 0.001671378095810078 42-43 7.428347092489236E-4 0.0 0.0 0.4067577159169793 0.001671378095810078 44-45 8.171181801738159E-4 0.0 0.0 0.5217299580409814 0.0017085198312725243 46-47 8.728307833674851E-4 0.0 0.0 0.6538431110809025 0.0017828033021974165 48-49 8.914016510987083E-4 0.0 0.0 0.780162153388682 0.001875657640853532 50-51 8.914016510987083E-4 0.0 0.0 0.9280976857356051 0.0019685119795096475 52-53 8.914016510987083E-4 0.0 0.0 1.0615293703844428 0.0020427954504345395 54-55 8.914016510987083E-4 0.0 0.0 1.2050821779467973 0.0020427954504345395 56-57 9.285433865611544E-4 0.0 0.0 1.3682272509655924 0.002098508053628209 58-59 9.285433865611544E-4 0.0 0.0 1.532523717783723 0.0021727915245531013 60-61 9.285433865611544E-4 0.0 0.0 1.715576761010389 0.0022285041277467707 62-63 9.285433865611544E-4 0.0 0.0 1.8920185753247396 0.002302787598671663 64-65 9.285433865611544E-4 0.0 0.0 2.0701874803380935 0.002321358466402886 66-67 9.656851220236006E-4 0.0 0.0 2.284773856972377 0.0023956419373277783 68-69 9.656851220236006E-4 0.0 0.0 2.513975506511132 0.0024513545405214477 70-71 0.001058539460679716 0.0 0.0 2.7431771560498874 0.002507067143715117 72-73 0.0011328229316046085 0.0 0.0 2.9917482206323083 0.002655634085564902 74-75 0.0011513937993358316 0.0 0.0 3.2360108439010857 0.0028227718951459096 76-77 0.0011699646670670547 0.0 0.0 3.4919174012373397 0.0028599136306083554 78-79 0.0011885355347982776 0.0 0.0 3.787101343825131 0.0028970553660708017 80-81 0.0013371024766480623 0.0 0.0 4.086482302520178 0.0028970553660708017 82-83 0.0013371024766480623 0.0 0.0 4.400924234945247 0.002971338836995694 84-85 0.0013371024766480623 0.0 0.0 4.732692786963549 0.0030084805724581404 86-87 0.0013371024766480623 0.0 0.0 5.065427024103872 0.0030827640433830324 88-89 0.0013371024766480623 0.0 0.0 5.408969506263768 0.003157047514307925 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16910 0.0 30.509022 1 AGGTAAG 2230 0.0 29.397903 2 GGTATCA 11685 0.0 29.35287 1 TAAGGTG 2510 0.0 28.384878 5 GTGTAAG 1105 0.0 28.374208 1 AAGGTAA 2395 0.0 26.77752 1 GTAAGGT 2765 0.0 25.767105 4 TAAGGTA 1255 0.0 25.357159 4 TATCAAC 20275 0.0 25.28149 2 ATCAACG 20590 0.0 24.823195 3 TCAACGC 20710 0.0 24.700464 4 CAACGCA 20850 0.0 24.489048 5 TGTAAGG 1285 0.0 24.399612 2 GGTAAGG 2885 0.0 24.03858 3 AACGCAG 21300 0.0 23.993973 6 GAGTACT 13555 0.0 22.460945 12-13 AGAGTAC 17545 0.0 22.320648 10-11 ACGCAGA 23610 0.0 21.545818 7 GGTGTGC 3545 0.0 21.437454 8 AGTACTT 14030 0.0 21.395819 12-13 >>END_MODULE