##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937968_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2699591 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.56991559091729 34.0 31.0 34.0 31.0 34.0 2 32.77495294657598 34.0 31.0 34.0 31.0 34.0 3 32.824553793519094 34.0 31.0 34.0 31.0 34.0 4 36.27011499149316 37.0 37.0 37.0 35.0 37.0 5 36.24615580656477 37.0 37.0 37.0 35.0 37.0 6 36.284769433591975 37.0 37.0 37.0 35.0 37.0 7 36.24638806396969 37.0 37.0 37.0 35.0 37.0 8 36.22707921311043 37.0 37.0 37.0 35.0 37.0 9 37.928237277424614 39.0 38.0 39.0 35.0 39.0 10-11 37.91027603811096 39.0 38.0 39.0 35.0 39.0 12-13 37.83905728682605 39.0 38.0 39.0 35.0 39.0 14-15 39.12735151361817 40.0 38.5 41.0 36.0 41.0 16-17 39.02726505607701 40.0 38.0 41.0 36.0 41.0 18-19 39.2823875913055 40.5 39.0 41.0 36.0 41.0 20-21 39.262606076253775 41.0 39.0 41.0 36.0 41.0 22-23 39.17688420208839 41.0 39.0 41.0 36.0 41.0 24-25 39.06610019814113 40.5 39.0 41.0 35.0 41.0 26-27 38.89032894242128 40.0 38.0 41.0 35.0 41.0 28-29 38.775818633267036 40.0 38.0 41.0 35.0 41.0 30-31 38.50736944966849 40.0 38.0 41.0 34.5 41.0 32-33 38.25435204814359 40.0 38.0 41.0 34.0 41.0 34-35 38.069428665305225 40.0 38.0 41.0 34.0 41.0 36-37 38.02269917924604 40.0 38.0 41.0 33.5 41.0 38-39 37.84875171831585 40.0 37.0 41.0 33.0 41.0 40-41 37.662349037317135 40.0 37.0 41.0 33.0 41.0 42-43 37.41168402917331 40.0 37.0 41.0 33.0 41.0 44-45 37.134329422494 40.0 36.0 41.0 32.0 41.0 46-47 36.71539466534004 40.0 36.0 41.0 31.0 41.0 48-49 36.26621014072131 39.0 35.0 41.0 30.0 41.0 50-51 35.8466984443199 39.0 35.0 41.0 28.5 41.0 52-53 35.32575193797875 38.5 35.0 40.0 27.0 41.0 54-55 34.99948844102681 38.0 34.5 40.0 26.5 41.0 56-57 34.63436535386286 38.0 34.0 40.0 26.0 41.0 58-59 34.18419456873282 37.5 34.0 40.0 24.5 41.0 60-61 33.70028126482863 37.0 33.0 40.0 23.0 41.0 62-63 33.25056795640525 36.0 33.0 39.0 22.0 41.0 64-65 32.76695414231267 36.0 32.0 39.0 20.5 40.0 66-67 32.22669248786205 35.0 31.5 38.5 18.5 40.0 68-69 31.66170468044974 35.0 31.0 38.0 15.5 40.0 70-71 31.049117255169396 35.0 30.5 37.0 10.0 39.5 72-73 30.499127830845488 34.0 30.0 36.5 7.5 39.0 74-75 29.93882091768716 34.0 29.0 36.0 6.0 39.0 76-77 28.591792793797282 32.5 28.0 35.0 2.0 36.5 78-79 29.26750255872093 34.0 29.0 35.0 2.0 37.0 80-81 28.984029617819886 34.0 29.0 35.0 2.0 37.0 82-83 28.455527152076 34.0 28.5 35.0 2.0 36.0 84-85 27.913112208478985 33.0 27.0 35.0 2.0 36.0 86-87 27.344804453711692 33.0 26.0 35.0 2.0 35.0 88-89 26.62298826007347 32.0 25.0 35.0 2.0 35.0 90-91 26.11256260670598 32.0 24.0 34.5 2.0 35.0 92-93 25.366123238668376 32.0 21.5 34.0 2.0 35.0 94-95 24.595810809859714 31.0 17.5 34.0 2.0 35.0 96-97 23.600392985455947 31.0 4.5 34.0 2.0 35.0 98-99 22.173555549711047 30.5 2.0 34.0 2.0 35.0 100-101 17.696277139759317 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12810016119831857 1101 2 0.15563031061446964 1101 3 0.11889047827419574 1101 4 0.10085747165000214 1101 5 0.1168773965658545 1101 6 0.11795878554128336 1101 7 0.1106908943664422 1101 8 0.1415424004393131 1101 9 0.048402836148980555 1101 10-11 0.0672507652446157 1101 12-13 0.11753591789958051 1101 14-15 0.0958271656508245 1101 16-17 0.1821321884401712 1101 18-19 0.17276885139628462 1101 20-21 0.19669655890368887 1101 22-23 0.26553744438596993 1101 24-25 0.2638916272570171 1101 26-27 0.25812862378554513 1101 28-29 0.33680360910140195 1101 30-31 0.32584319039332854 1101 32-33 0.3418817716578886 1101 34-35 0.21353539526885612 1101 36-37 0.3613398998756452 1101 38-39 0.2888740933889906 1101 40-41 0.24133850033604887 1101 42-43 0.34283544015405454 1101 44-45 0.40373503244640574 1101 46-47 0.3683739165906985 1101 48-49 0.4758117946248106 1101 50-51 0.24640111054355174 1101 52-53 0.07782583290152445 1101 54-55 0.14837438203478115 1101 56-57 0.12698096196812259 1101 58-59 0.3356378119229291 1101 60-61 0.4060359484486753 1101 62-63 0.38083007857459705 1101 64-65 0.34961991360653855 1101 66-67 0.24754633877530807 1101 68-69 0.23948713397811616 1101 70-71 0.16746501695051919 1101 72-73 0.07312475333117519 1101 74-75 -0.08356234290098996 1101 76-77 -0.11337959123468622 1101 78-79 -0.20804473678494517 1101 80-81 -0.24737696888655947 1101 82-83 -0.15861139325897256 1101 84-85 -0.23047472445033534 1101 86-87 -0.33095477836160825 1101 88-89 -0.24433308886069227 1101 90-91 -0.36513427250813635 1101 92-93 -0.6780132357652988 1101 94-95 -0.6899630975069435 1101 96-97 -0.34241744450864076 1101 98-99 -0.41371915285859373 1101 100-101 -0.8998257978854944 1102 1 0.09903187387240564 1102 2 0.1319786216156018 1102 3 0.09595760323229996 1102 4 0.06577464402985811 1102 5 0.0672127361127437 1102 6 0.06544133219644976 1102 7 0.12387770755325533 1102 8 0.07055666975208652 1102 9 0.10998337112341261 1102 10-11 0.1628948275418196 1102 12-13 0.18400838119229235 1102 14-15 0.1947216682928925 1102 16-17 0.21135308111012563 1102 18-19 0.2008426189655168 1102 20-21 0.14880860973411103 1102 22-23 0.22180959533690014 1102 24-25 0.2227440742908442 1102 26-27 0.1645886789801807 1102 28-29 0.11761277397934577 1102 30-31 0.08149698662953142 1102 32-33 -0.020736337034200858 1102 34-35 0.09905357598496067 1102 36-37 0.14776078755383537 1102 38-39 0.1113631471645462 1102 40-41 0.20231957798649347 1102 42-43 0.23123903850249405 1102 44-45 0.07249416915654905 1102 46-47 -0.0744577326166791 1102 48-49 -0.09893246662983302 1102 50-51 0.004108928358441233 1102 52-53 -0.044412652169540934 1102 54-55 0.010677920482059733 1102 56-57 0.153447827354519 1102 58-59 0.027959588135033187 1102 60-61 -0.04582214640909399 1102 62-63 0.10981894337624709 1102 64-65 -0.0572435830525464 1102 66-67 -0.13443259241679328 1102 68-69 -0.14021822717598198 1102 70-71 -0.00852433276530462 1102 72-73 -0.12186003961760505 1102 74-75 -0.2531240838855098 1102 76-77 -0.30555919132310905 1102 78-79 -0.1009931576048757 1102 80-81 -0.16649105564340871 1102 82-83 -0.26784918661422097 1102 84-85 -0.3925005069092755 1102 86-87 -0.4786685506373516 1102 88-89 -0.5664936667642912 1102 90-91 -0.7276234867332363 1102 92-93 -0.6542461122334124 1102 94-95 -0.7329320202130454 1102 96-97 -1.0503342032852316 1102 98-99 -1.3037439155715482 1102 100-101 -1.390897226456925 1103 1 0.040587194551584105 1103 2 0.06168571284639057 1103 3 0.08479748104796414 1103 4 0.09043028274927423 1103 5 0.0726229068371822 1103 6 0.05656635509917152 1103 7 -0.023207014756323474 1103 8 0.0363721298439188 1103 9 0.05602591686733405 1103 10-11 0.11860112709032933 1103 12-13 0.09184234395177526 1103 14-15 0.0571545667251101 1103 16-17 0.06424135409421439 1103 18-19 0.03702745558361897 1103 20-21 0.06037024746357389 1103 22-23 0.15761222990237655 1103 24-25 0.19140750540627494 1103 26-27 0.20529810287066397 1103 28-29 0.11516730981288248 1103 30-31 0.13165651949597645 1103 32-33 0.10633755604630579 1103 34-35 0.11446881949220966 1103 36-37 0.0689649855208927 1103 38-39 0.014203119325770786 1103 40-41 -0.038585818032395025 1103 42-43 -0.014637445389034554 1103 44-45 0.050412694248358036 1103 46-47 -0.00976915499215636 1103 48-49 -0.08901062987226283 1103 50-51 -0.14220427974598238 1103 52-53 -0.07994229991877688 1103 54-55 -0.2040930727715491 1103 56-57 -0.12304789154494955 1103 58-59 -0.23830920919156284 1103 60-61 -0.2855865081272242 1103 62-63 -0.3141093116138123 1103 64-65 -0.43049408827983626 1103 66-67 -0.22982056458896238 1103 68-69 -0.17473041279334112 1103 70-71 -0.23821354619683177 1103 72-73 -0.4529426958962297 1103 74-75 -0.6488122526792814 1103 76-77 -0.4472469034296971 1103 78-79 -0.4888087708373199 1103 80-81 -0.5244433947295413 1103 82-83 -0.5127282055298252 1103 84-85 -0.6537896705701023 1103 86-87 -0.9339624302086662 1103 88-89 -0.9720858970658988 1103 90-91 -0.9424206301138689 1103 92-93 -0.9602417381546502 1103 94-95 -0.6910586592961714 1103 96-97 -0.6620232758820848 1103 98-99 -0.9035493791363898 1103 100-101 -1.4459360653776123 1104 1 0.07976904681716945 1104 2 0.14306670343240313 1104 3 0.05771196440490911 1104 4 0.05725773058336614 1104 5 0.043754913741878454 1104 6 0.09210232004849672 1104 7 0.06398845752315907 1104 8 0.08798173295179623 1104 9 0.11583228848488858 1104 10-11 0.14110041371586846 1104 12-13 0.17230858374540503 1104 14-15 0.1993569680139231 1104 16-17 0.11900691941307429 1104 18-19 0.12295420898126252 1104 20-21 0.15376775890636196 1104 22-23 0.11365892617888562 1104 24-25 0.1427110612740563 1104 26-27 0.07971460734861324 1104 28-29 0.00540777138855475 1104 30-31 0.023138988398599736 1104 32-33 0.011714032219593662 1104 34-35 0.13005349224206952 1104 36-37 0.11153184758178014 1104 38-39 0.2264144265988648 1104 40-41 0.18117286306299007 1104 42-43 0.20459964061385705 1104 44-45 0.1859392861610658 1104 46-47 0.2206535145766253 1104 48-49 0.28169760701156576 1104 50-51 0.316542522864907 1104 52-53 0.20544603170629472 1104 54-55 0.21427225684583817 1104 56-57 0.30129458833661005 1104 58-59 0.3087141638987916 1104 60-61 0.03192494745098173 1104 62-63 0.09940571147648569 1104 64-65 0.08772975007143202 1104 66-67 0.1037266211328074 1104 68-69 0.12468964256195747 1104 70-71 -0.07802275211828658 1104 72-73 0.014146589155405564 1104 74-75 -0.10369307755768631 1104 76-77 -0.15352168759393336 1104 78-79 -0.13317528791927913 1104 80-81 -0.10856223971551415 1104 82-83 -0.22478964018664271 1104 84-85 -0.26985937397267534 1104 86-87 -0.49250967991019934 1104 88-89 -0.4988307214222303 1104 90-91 -0.8661760955859066 1104 92-93 -0.7174402622692675 1104 94-95 -0.7212655890732549 1104 96-97 -0.8930166450184984 1104 98-99 -0.7941105393199841 1104 100-101 -1.3520670230140475 1105 1 0.10353939199362117 1105 2 0.12925822382230479 1105 3 0.12250585010112047 1105 4 0.07162685389418044 1105 5 0.02283221208488584 1105 6 0.01235971266731184 1105 7 0.0863478641944937 1105 8 0.05620274207492315 1105 9 0.0850795154753925 1105 10-11 0.06451538838176418 1105 12-13 0.10365727391823754 1105 14-15 0.03703891807072779 1105 16-17 0.051108169355323696 1105 18-19 0.10531333133250342 1105 20-21 0.15544598792383368 1105 22-23 0.10746237687718718 1105 24-25 0.0838039344353021 1105 26-27 0.06183131719638624 1105 28-29 0.07677389393762724 1105 30-31 0.1251336419960225 1105 32-33 0.204042068906368 1105 34-35 0.24471638337471546 1105 36-37 0.2231972348079907 1105 38-39 0.12211988992808642 1105 40-41 0.21995738278118182 1105 42-43 0.2209881770642923 1105 44-45 0.251505426717074 1105 46-47 0.2398668897050129 1105 48-49 0.3240439764215637 1105 50-51 0.20294449936348258 1105 52-53 0.18530683924645786 1105 54-55 0.20790881756772706 1105 56-57 0.27314438084217585 1105 58-59 0.25530300386093785 1105 60-61 0.141557594032804 1105 62-63 0.27698938155144504 1105 64-65 0.2518048093936329 1105 66-67 -0.014452954258814543 1105 68-69 0.062229402951192725 1105 70-71 0.01595940561065845 1105 72-73 0.08335735801619748 1105 74-75 0.032522906207635316 1105 76-77 0.062357678940880845 1105 78-79 0.0025952610199091453 1105 80-81 -0.23101046680197967 1105 82-83 -0.05993655075256754 1105 84-85 -0.1777664640867478 1105 86-87 -0.1854928374981739 1105 88-89 -0.45548523340454494 1105 90-91 -0.5018016708898614 1105 92-93 -0.45536006386122097 1105 94-95 -0.7007274599706044 1105 96-97 -0.8112116803156582 1105 98-99 -1.0125156160923332 1105 100-101 -1.4436508222177125 1106 1 0.14481250044146776 1106 2 0.16965473527656627 1106 3 0.15640675197462173 1106 4 0.07871558865312522 1106 5 0.114800553745674 1106 6 0.021994845330176815 1106 7 0.10476189595238594 1106 8 0.10530671901964439 1106 9 0.10176782770941628 1106 10-11 0.15199973601841776 1106 12-13 0.1510269657711234 1106 14-15 0.1932782717162027 1106 16-17 0.20613250526522364 1106 18-19 0.28973146421969176 1106 20-21 0.28548359238335763 1106 22-23 0.36178443318238607 1106 24-25 0.32091485563142896 1106 26-27 0.28439952012730174 1106 28-29 0.25692928303744367 1106 30-31 0.31968758923600404 1106 32-33 0.31556899614299994 1106 34-35 0.32515595539369 1106 36-37 0.26942576674721863 1106 38-39 0.1140173474735775 1106 40-41 0.11102162066219279 1106 42-43 0.1262930757287748 1106 44-45 0.029586590806850666 1106 46-47 0.07349737821390079 1106 48-49 -0.012510926283276547 1106 50-51 0.05356891310692191 1106 52-53 -0.058880534007379026 1106 54-55 -0.19359911026946008 1106 56-57 -0.1204837093076847 1106 58-59 -0.11923437466153786 1106 60-61 -0.07755205881280602 1106 62-63 -0.0866938062103273 1106 64-65 -0.12282905297934121 1106 66-67 -0.271621849804788 1106 68-69 -0.5891393748101379 1106 70-71 -0.8789501258301478 1106 72-73 -0.8592370135329297 1106 74-75 -0.5134297497972149 1106 76-77 -0.5791745945030833 1106 78-79 -0.5810683584594187 1106 80-81 -0.8048377435784282 1106 82-83 -0.759762524269771 1106 84-85 -0.905423422463258 1106 86-87 -0.8918683998357899 1106 88-89 -1.0701473201320546 1106 90-91 -1.164653481392346 1106 92-93 -0.8595370550002208 1106 94-95 -0.8442970456750913 1106 96-97 -0.9578714966828059 1106 98-99 -1.1651380374523406 1106 100-101 -1.781215330641384 1107 1 0.012074473240069494 1107 2 0.02748173524125974 1107 3 0.01490490010836254 1107 4 0.051435794372160615 1107 5 0.049692000092420585 1107 6 -0.002398383464019105 1107 7 0.005555245152301325 1107 8 -0.017443977113309472 1107 9 -0.07519356765639884 1107 10-11 0.05990626838762125 1107 12-13 0.018675679099594333 1107 14-15 0.09357072834590241 1107 16-17 -0.05726861513031878 1107 18-19 0.04662337495428659 1107 20-21 -0.04005528603619268 1107 22-23 0.01981005470508279 1107 24-25 0.0855879559050976 1107 26-27 0.08650311931270949 1107 28-29 0.03295250721767928 1107 30-31 -0.004341729436397657 1107 32-33 -0.013130981176196599 1107 34-35 0.03911188761939144 1107 36-37 -0.007053467156453053 1107 38-39 -0.00628124912957162 1107 40-41 -0.006806175224980393 1107 42-43 -0.05241231745394259 1107 44-45 0.015347507661680027 1107 46-47 -0.012748004089203846 1107 48-49 -0.0054107591395506915 1107 50-51 0.039968064043144125 1107 52-53 0.0570541034020593 1107 54-55 0.07445202266335826 1107 56-57 0.07964277055457814 1107 58-59 0.14603566747447871 1107 60-61 0.0305541124589368 1107 62-63 0.048190219505748644 1107 64-65 -0.10149907847878836 1107 66-67 -0.03498392212361523 1107 68-69 -0.08959119546785743 1107 70-71 -0.39175355631589426 1107 72-73 -0.40106932283918084 1107 74-75 -0.28229176616209983 1107 76-77 -0.3755874975435667 1107 78-79 -0.4591831532221313 1107 80-81 -0.4419313518421397 1107 82-83 -0.4348274697490808 1107 84-85 -0.5756634619122885 1107 86-87 -0.6276391909169092 1107 88-89 -0.7910538100121691 1107 90-91 -0.9406704219740405 1107 92-93 -0.7217470145637712 1107 94-95 -0.8746040383627793 1107 96-97 -0.8952397281250981 1107 98-99 -1.2629458395273652 1107 100-101 -1.8643117324134764 1108 1 -0.02771950589829686 1108 2 -0.06231786736687184 1108 3 -0.026152677493122667 1108 4 -0.03037920212398859 1108 5 -0.014544126038643412 1108 6 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-0.4363870231372289 2316 50-51 -0.34847875964408104 2316 52-53 -0.29507437320230423 2316 54-55 -0.31648059558859387 2316 56-57 -0.3608075770327659 2316 58-59 -0.2104906891101379 2316 60-61 -0.0749625369042306 2316 62-63 -0.03138798994539371 2316 64-65 0.014767909367556342 2316 66-67 0.046434750454352525 2316 68-69 0.14258205863967177 2316 70-71 0.32295878936208666 2316 72-73 0.380411387587106 2316 74-75 0.625189254975453 2316 76-77 0.4760332542941832 2316 78-79 0.4340546377877921 2316 80-81 0.5297822291637182 2316 82-83 0.6365121927950916 2316 84-85 0.7294357944687775 2316 86-87 0.7062381307519416 2316 88-89 0.9964849349314662 2316 90-91 0.9910140256740334 2316 92-93 1.2565518495860815 2316 94-95 1.458483245611454 2316 96-97 1.8351965288732153 2316 98-99 2.2352261216203644 2316 100-101 2.7638256579292495 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 26.0 5 44.0 6 107.0 7 243.0 8 712.0 9 1345.0 10 2310.0 11 3812.0 12 6201.0 13 8832.0 14 11875.0 15 16812.0 16 22029.0 17 30948.0 18 31773.0 19 25146.0 20 22767.0 21 21845.0 22 22645.0 23 24193.0 24 27993.0 25 31464.0 26 35761.0 27 41731.0 28 50043.0 29 60495.0 30 73790.0 31 91196.0 32 114746.0 33 150666.0 34 209367.0 35 304280.0 36 436421.0 37 529179.0 38 274841.0 39 13949.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.53187901437451 18.55034634109838 14.931120761128 22.986653883399114 2 15.784559949407251 20.237729609614107 38.96282640677741 25.014884034201234 3 19.217193252567853 23.526546138349477 30.50918410365801 26.747076505424662 4 12.972493220264269 16.81107753809932 36.55837981263074 33.65804942900568 5 13.363528332723359 37.402410166975116 34.262755022662915 14.97130647763861 6 32.418821535176654 36.50276750238744 16.292591420927817 14.785819541508092 7 28.48772276985662 33.64980102541459 19.95991244599645 17.90256375873234 8 27.49683192750309 33.31708395827368 19.46613394399374 19.71995017022949 9 26.683893967641765 16.405225828653304 20.039035542791485 36.87184466091345 10-11 25.10170985160345 26.224157659438042 26.74568110502665 21.92845138393186 12-13 26.74179354351893 23.66742956693232 27.152581720065054 22.438195169483695 14-15 22.66810046410734 25.183185156566307 25.96504433449363 26.18367004483272 16-17 21.79361682226546 28.318301326070305 27.334440321279637 22.553641530384596 18-19 22.08525291423775 26.19517178713368 30.054200803010527 21.66537449561804 20-21 22.033022780117435 25.256307344334754 30.740471426968014 21.970198448579804 22-23 22.049414151995617 25.241119858526716 30.912516007054403 21.796949982423264 24-25 22.158936629164536 25.57961852808548 30.553185767242784 21.708259075507197 26-27 21.188413888972764 25.913789401671277 30.4935225066796 22.404274202676362 28-29 21.609643092444333 26.821290982577956 30.083782672936398 21.485283252041317 30-31 21.49518273597481 25.902335220528027 29.781317680119297 22.821164363377868 32-33 21.083175192093915 26.03577727144593 30.01571349141407 22.865334045046083 34-35 22.676713099124857 26.09485380277276 29.443051301932737 21.785381796169645 36-37 22.690381316424954 25.28244323445706 29.707760757365342 22.319414691752645 38-39 21.8247587754433 25.821506330321363 29.62384805376536 22.729886840469973 40-41 22.382569509978154 25.770779832359242 29.700882285663482 22.14576837199912 42-43 22.043219871539126 25.883173136941334 29.20756777461299 22.866039216906543 44-45 22.00511114461413 26.042722768004488 29.82822212698146 22.123943960399927 46-47 22.96583198945468 26.732242141527973 28.434576925021666 21.867348943995683 48-49 22.31290921897622 26.773772275931222 28.202458272652564 22.710860232439988 50-51 22.446343035981105 26.960460813565838 28.643095640596833 21.950100509856227 52-53 23.22884431189522 26.35362377469299 28.38137131398404 22.036160599427756 54-55 21.960358367265137 26.85270611961616 28.70842678741406 22.47850872570464 56-57 22.4447201664508 26.91888191062754 28.229003962690836 22.407393960230827 58-59 22.428371540797578 26.336242358090335 28.06526233927235 23.17012376183974 60-61 21.494495341984486 26.9292511742511 28.86156311579999 22.714690367964426 62-63 21.836141409908784 26.970687707836287 28.953856476415474 22.23931440583945 64-65 21.2608909976768 27.290214742047787 28.473212401802023 22.975681858473394 66-67 21.249092576549124 27.038579227259586 28.54295254983443 23.16937564635687 68-69 22.265838201842516 26.422749014657214 28.757799279459405 22.55361350404086 70-71 22.023354909675195 26.903641500895365 27.81819855730018 23.254805032129255 72-73 21.835076461673587 26.98603830325877 28.167961186268204 23.010924048799446 74-75 21.846882101111024 27.792221456763837 27.792110311959384 22.568786130165755 76-77 22.053438461739386 26.839773349756406 28.218185038171782 22.888603150332422 78-79 22.20248608620937 27.30650651496272 28.03972002352034 22.451287375307576 80-81 22.714665944505334 27.285324793849675 27.531184421764937 22.46882483988005 82-83 22.28911030796825 27.378943259235406 27.825491018187066 22.506455414609274 84-85 22.046097578190434 27.768345395975714 27.8040803835296 22.38147664230426 86-87 22.645934523971633 27.786247577399546 26.96992316412693 22.59789473450189 88-89 22.110062357714796 28.014677154653466 26.657371950495445 23.21788853713629 90-91 21.995457416989485 27.76413026764558 26.968381621435363 23.272030693929572 92-93 22.403662555930655 27.186434033922698 27.383767146926658 23.026136263219993 94-95 21.892835006840862 26.69723968507951 27.848201388474674 23.56172391960495 96-97 22.17332720313781 26.126154829918562 27.980588372807276 23.719929594136353 98-99 21.815100889877375 26.976856445799278 27.03237074587176 24.175671918451584 100-101 22.05197814121816 25.899992850069665 27.566500835208185 24.48152817350399 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1163.0 1 803.5 2 651.5 3 873.0 4 724.5 5 694.5 6 1361.5 7 2932.5 8 4830.0 9 5508.0 10 4401.5 11 3252.0 12 3075.0 13 3237.0 14 3491.5 15 3649.0 16 3799.0 17 4106.0 18 4531.0 19 5022.5 20 5640.0 21 6736.5 22 8054.0 23 9859.0 24 12292.0 25 15380.5 26 19251.0 27 23460.0 28 27222.5 29 30609.5 30 34639.0 31 41946.0 32 54734.5 33 56729.5 34 49612.0 35 49772.5 36 53560.0 37 58176.5 38 61658.5 39 63892.0 40 68064.0 41 74641.0 42 78150.5 43 78837.0 44 80714.5 45 88302.5 46 92136.0 47 96878.0 48 103766.5 49 99338.0 50 93016.0 51 89232.5 52 88051.5 53 92470.5 54 99005.5 55 95291.0 56 86901.5 57 80742.0 58 76286.0 59 67173.0 60 50994.0 61 41965.5 62 37871.0 63 28083.0 64 17876.0 65 12855.0 66 9598.5 67 7680.0 68 6205.5 69 4719.0 70 3612.0 71 2551.0 72 1806.5 73 1338.0 74 914.0 75 614.0 76 438.0 77 281.0 78 186.0 79 114.5 80 62.0 81 30.0 82 20.0 83 13.5 84 5.5 85 2.5 86 2.5 87 2.5 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008482766463512436 2 0.014446632841789736 3 0.00937179002300719 4 7.408529662456277E-5 5 0.0010001515044315973 6 0.001666919174052662 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.075448836508938E-4 14-15 0.0 16-17 0.0027226346509526813 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0037042648312281376 26-27 0.01905844255666877 28-29 0.00616760094399485 30-31 0.0030189758374509325 32-33 0.0 34-35 0.009557003264568596 36-37 8.149382628701903E-4 38-39 0.0030930611340754953 40-41 0.0025374214093912746 42-43 0.003407923644729887 44-45 0.0 46-47 1.666919174052662E-4 48-49 4.630331039035172E-4 50-51 0.003870956748633404 52-53 0.008019733359608918 54-55 0.00672324066867907 56-57 0.015372699049596771 58-59 0.021355086752030215 60-61 0.003870956748633404 62-63 0.018576888128609113 64-65 0.01909548520498105 66-67 0.012131467322272152 68-69 0.029041436276828602 70-71 0.015391220373752913 72-73 0.004963714873845705 74-75 0.015168964483879225 76-77 0.02009563670941265 78-79 0.024077721402982893 80-81 0.010612718741468616 82-83 0.03287535037714972 84-85 0.020577191137472306 86-87 0.029134042897609305 88-89 0.020984660268907402 90-91 0.025411256742225028 92-93 0.02228115295983725 94-95 0.01370577987554411 96-97 0.014224376951916048 98-99 0.010612718741468616 100-101 0.009779259154442284 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2699591.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.483062823203966 #Duplication Level Percentage of deduplicated Percentage of total 1 67.57699000304706 26.68146541693333 2 15.655375666739344 12.362443619414563 3 6.373012346403706 7.548781405383361 4 3.2870897488954713 5.191374842445986 5 1.8815772435398126 3.714521625669668 6 1.1512018884803954 2.727178589103749 7 0.802499097643313 2.2179585601470793 8 0.5679758087096829 1.7940339629875588 9 0.4231431395273653 1.5036288445050052 >10 2.0443807733403365 14.237956691761669 >50 0.14039351645921305 3.8199815808137845 >100 0.07877805812128907 5.9824539288764536 >500 0.008650316773090841 2.4008476140566146 >1k 0.008086165679192703 7.032700442418069 >5k 6.581762762133595E-4 1.789563471039406 >10k+ 1.8805036463238843E-4 0.9951094044437564 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14793 0.5479718964835785 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12012 0.44495629152712396 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7914 0.29315551874339485 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 7276 0.26952230912015934 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 7189 0.2662995987169908 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 7084 0.26241012064420133 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 6507 0.24103651256801492 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 6471 0.2397029772287728 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5764 0.2135138248719899 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4927 0.18250912823461035 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4876 0.18061995317068402 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4843 0.1793975457763787 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4662 0.1726928264318558 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 4497 0.16658078946032936 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4060 0.1503931521478624 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3892 0.14416998723139912 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3776 0.1398730400271745 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3696 0.136909628162192 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 3617 0.13398325894552174 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3554 0.13164957210184802 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3540 0.13113097502547608 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3465 0.12835277640205497 No Hit TCTTAGGAGTGGGGGTGGCTTTTGGGAGGGTGAGGGACTTCCTGTAACCA 3412 0.12638951604150409 No Hit CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT 3377 0.12509302335057423 No Hit TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG 3282 0.12157397176090748 No Hit GGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTG 3237 0.11990705258685481 No Hit GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA 3229 0.11961071140035658 No Hit CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA 3190 0.11816604811617759 No Hit TTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCTCCCA 3115 0.1153878494927565 No Hit GCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTT 3011 0.11153541406827923 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2905 0.10760889334717741 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2790 0.10334898879126504 No Hit GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG 2766 0.10245996523177028 No Hit GCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTA 2710 0.10038557692628254 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.704264831228138E-5 6 0.0 0.0 0.0 0.0 3.704264831228138E-5 7 0.0 0.0 0.0 0.0 7.408529662456276E-5 8 0.0 0.0 0.0 0.0 7.408529662456276E-5 9 0.0 0.0 0.0 0.0 7.408529662456276E-5 10-11 0.0 0.0 0.0 0.0 1.1112794493684414E-4 12-13 0.0 0.0 0.0 0.0 7.778956145579089E-4 14-15 0.0 0.0 0.0 3.704264831228138E-5 8.890235594947531E-4 16-17 0.0 0.0 0.0 9.260662078070344E-5 9.631088561193158E-4 18-19 1.852132415614069E-5 0.0 0.0 1.1112794493684414E-4 0.0011298007735245819 20-21 3.704264831228138E-5 0.0 0.0 1.4817059324912554E-4 0.001426141960022833 22-23 7.408529662456276E-5 0.0 0.0 4.0746913143509516E-4 0.0016483978498965214 24-25 3.333838348105324E-4 0.0 0.0 8.890235594947531E-4 0.001666919174052662 26-27 3.333838348105324E-4 0.0 0.0 0.0016669191740526623 0.0017039618223649434 28-29 4.4451177974737656E-4 0.0 0.0 0.005519354598529926 0.0017039618223649434 30-31 5.556397246842206E-4 0.0 0.0 0.016965532927024872 0.0017224831465210842 32-33 5.556397246842206E-4 0.0 0.0 0.04254348158665516 0.0018336110914579282 34-35 5.92682372996502E-4 0.0 0.0 0.07875267031191022 0.001852132415614069 36-37 6.297250213087834E-4 0.0 0.0 0.13561313547126214 0.001852132415614069 38-39 6.297250213087834E-4 0.0 0.0 0.22397837302020934 0.0018891750639263504 40-41 6.297250213087834E-4 0.0 0.0 0.31380679517749166 0.0018891750639263504 42-43 6.667676696210648E-4 0.0 0.0 0.40345000409321263 0.0018891750639263504 44-45 7.038103179333462E-4 0.0 0.0 0.5156521858311129 0.001981781684707054 46-47 7.593742904017683E-4 0.0 0.0 0.6468016821807452 0.0020743883054877574 48-49 7.778956145579089E-4 0.0 0.0 0.7799885241875528 0.0021484736021123198 50-51 7.778956145579089E-4 0.0 0.0 0.9281220747883661 0.0022225588987368826 52-53 7.778956145579089E-4 0.0 0.0 1.0649390963297773 0.002259601547049164 54-55 7.778956145579089E-4 0.0 0.0 1.2094054247476747 0.002259601547049164 56-57 7.778956145579089E-4 0.0 0.0 1.3762084700978776 0.0022966441953614454 58-59 7.778956145579089E-4 0.0 0.0 1.541585373488058 0.002333686843673727 60-61 7.778956145579089E-4 0.0 0.0 1.7244834495299473 0.002333686843673727 62-63 7.778956145579089E-4 0.0 0.0 1.9017695643525259 0.0024818574369228526 64-65 7.778956145579089E-4 0.0 0.0 2.0812226741013733 0.0025003787610789934 66-67 8.149382628701903E-4 0.0 0.0 2.2952921386980476 0.0025189000852351338 68-69 8.149382628701903E-4 0.0 0.0 2.524604653075225 0.0025189000852351338 70-71 8.519809111824717E-4 0.0 0.0 2.7526577174097855 0.0025189000852351338 72-73 8.890235594947531E-4 0.0 0.0 2.9976207506989025 0.0027041133267965405 74-75 9.260662078070345E-4 0.0 0.0 3.2387128272393855 0.0029263692166702293 76-77 9.260662078070345E-4 0.0 0.0 3.498807782364069 0.0030004545132947917 78-79 9.260662078070345E-4 0.0 0.0 3.795778693883629 0.003093061134075495 80-81 0.0010371941527438787 0.0 0.0 4.09999144314824 0.0032227104031684797 82-83 0.0010371941527438787 0.0 0.0 4.4108718691090605 0.003296795699793043 84-85 0.0010371941527438787 0.0 0.0 4.736939780877918 0.003296795699793043 86-87 0.0010371941527438787 0.0 0.0 5.066749000126316 0.0034449662930421685 88-89 0.0010371941527438787 0.0 0.0 5.4081340469723 0.0035931368862912936 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 17805 0.0 31.134367 1 GGTATCA 12260 0.0 30.72514 1 TAAGGTG 2520 0.0 28.832972 5 AGGTAAG 2260 0.0 28.372412 2 GTGTAAG 1005 0.0 28.359354 1 GTAAGGT 2700 0.0 26.383112 4 TATCAAC 21365 0.0 25.788494 2 ATCAACG 21630 0.0 25.448233 3 AAGGTAA 2475 0.0 25.334354 1 TCAACGC 21775 0.0 25.233278 4 CAACGCA 21845 0.0 25.1959 5 AACGCAG 22240 0.0 24.769754 6 GAGTACT 13800 0.0 24.519997 12-13 TGTAAGG 1180 0.0 24.151281 2 GGTAAGG 2865 0.0 24.036657 3 TAAGGTA 1220 0.0 23.7448 4 AGAGTAC 17790 0.0 23.077864 10-11 GTACTTT 14685 0.0 22.783142 14-15 GTACATG 8290 0.0 22.461702 1 ACGCAGA 24320 0.0 22.45602 7 >>END_MODULE