FastQCFastQC Report
Thu 26 May 2016
SRR937967_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937967_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581083
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106520.673715421644531No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89350.5651189722487687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56200.3554525600490297No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22050.1394613692007314No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17360.10979815733898853No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16950.10720499809307923No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16720.10575029900391061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39850.056.3367921
GTATCAA70400.043.8734551
TGGTATC15550.034.8456542
TATCAAC91000.033.9504932
ATCAACG91900.033.6697273
TCAACGC92150.033.5268064
CAACGCA94000.032.8669665
GTGGTAT17000.032.2439651
AACGCAG97200.031.828796
ACGCAGA110500.027.728367
CGCAGAG111400.027.4190588
GTACATG73050.025.3821771
TACATGG70650.025.3631572
GCAGAGT118600.025.1937439
ACATGGG73550.023.8461273
GAGTACT75850.022.98279612-13
TACCTGG11650.022.8473722
CAGAGTA113950.022.42892310-11
GTAAGGT9200.022.2154314
TAAGGTG9300.021.9765575
Produced by FastQC (version 0.11.5)