FastQCFastQC Report
Thu 26 May 2016
SRR937967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937967_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581083
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103610.6553103157772235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90260.5708745208189576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54400.344067958481623No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23190.14667161686008895No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18850.11922207752534181No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16640.10524431671202587No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16560.10473833442014113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38000.058.5599861
GTATCAA70150.043.5834921
TGGTATC14850.033.9404952
ATCAACG91150.033.1750563
TATCAAC91850.032.924312
TCAACGC92650.032.6358874
CAACGCA94050.032.099535
AACGCAG97550.031.2086356
GTGGTAT16700.030.4653421
ACGCAGA111600.027.1519187
CGCAGAG112350.026.8861168
GTACCGT1351.3443323E-524.6268186
GCAGAGT123450.024.160879
TACATGG69400.023.9798972
TACCGTA1000.001655018823.7472887
GTACATG70850.023.6904661
TAAGGTG8850.023.6370435
GAGTACT78400.022.77801312-13
ACATGGG71000.022.3665683
TACCTGG11150.021.7487372
Produced by FastQC (version 0.11.5)