##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937967_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1581083 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50323797042913 33.0 31.0 34.0 31.0 34.0 2 32.727594945995875 34.0 31.0 34.0 31.0 34.0 3 32.78274258846626 34.0 31.0 34.0 31.0 34.0 4 36.23577826085032 37.0 35.0 37.0 35.0 37.0 5 36.1686742568227 37.0 35.0 37.0 35.0 37.0 6 36.22379723265635 37.0 37.0 37.0 35.0 37.0 7 36.200408833691846 37.0 37.0 37.0 35.0 37.0 8 36.196014377486826 37.0 37.0 37.0 35.0 37.0 9 37.8894947324081 39.0 38.0 39.0 35.0 39.0 10-11 37.86607597450609 39.0 38.0 39.0 35.0 39.0 12-13 37.78596569566557 39.0 38.0 39.0 35.0 39.0 14-15 39.024388030229915 40.0 38.0 41.0 36.0 41.0 16-17 38.934449361608465 40.0 38.0 41.0 35.5 41.0 18-19 39.196118736334526 40.5 39.0 41.0 36.0 41.0 20-21 39.17788724564112 40.0 39.0 41.0 36.0 41.0 22-23 39.06865452351332 40.0 39.0 41.0 35.5 41.0 24-25 38.95666071926648 40.0 38.0 41.0 35.0 41.0 26-27 38.790844629915064 40.0 38.0 41.0 35.0 41.0 28-29 38.662457948127965 40.0 38.0 41.0 34.5 41.0 30-31 38.40477002156117 40.0 38.0 41.0 34.0 41.0 32-33 38.14893968248346 40.0 37.5 41.0 34.0 41.0 34-35 38.02406483404097 40.0 37.0 41.0 33.0 41.0 36-37 37.95274030522117 40.0 37.0 41.0 33.0 41.0 38-39 37.75093780655412 40.0 37.0 41.0 33.0 41.0 40-41 37.51880451563896 40.0 37.0 41.0 33.0 41.0 42-43 37.24282438050375 40.0 36.5 41.0 32.0 41.0 44-45 36.94178578860186 40.0 36.0 41.0 31.0 41.0 46-47 36.522121229562266 39.0 35.5 41.0 30.5 41.0 48-49 36.065751766352555 39.0 35.0 41.0 29.5 41.0 50-51 35.66324348563611 39.0 35.0 40.0 28.0 41.0 52-53 35.130773969488004 38.0 34.5 40.0 26.5 41.0 54-55 34.75034232864435 38.0 34.0 40.0 26.0 41.0 56-57 34.36635584596128 38.0 34.0 40.0 25.0 41.0 58-59 33.85944254665947 37.0 33.0 40.0 23.0 41.0 60-61 33.38274556111223 37.0 33.0 39.5 22.0 41.0 62-63 32.828737011276445 36.0 32.0 39.0 19.5 40.5 64-65 32.260752914299886 35.5 31.0 39.0 17.5 40.0 66-67 31.697809349667285 35.0 31.0 38.0 12.5 40.0 68-69 31.074566610355056 35.0 30.0 38.0 8.0 40.0 70-71 30.454511243242763 34.5 29.0 37.0 6.0 39.5 72-73 29.792962165806603 34.0 29.0 36.5 2.0 39.0 74-75 29.14534942188361 34.0 28.0 36.0 2.0 39.0 76-77 27.83466522630374 32.0 26.5 34.5 2.0 36.5 78-79 28.476302635598508 33.5 28.0 35.0 2.0 37.0 80-81 28.20315410386425 34.0 27.0 35.0 2.0 37.0 82-83 27.64731168445932 33.0 26.0 35.0 2.0 36.0 84-85 27.001974912133015 33.0 25.5 35.0 2.0 36.0 86-87 26.299535192017117 32.5 24.5 35.0 2.0 35.5 88-89 25.499904495842408 32.0 20.5 34.5 2.0 35.0 90-91 24.85388338246632 31.5 18.5 34.0 2.0 35.0 92-93 23.916142606049142 31.0 8.5 34.0 2.0 35.0 94-95 23.02498002951142 30.5 2.0 34.0 2.0 35.0 96-97 21.983283293792926 30.0 2.0 34.0 2.0 35.0 98-99 20.581710764077535 29.0 2.0 34.0 2.0 35.0 100-101 16.733273332266556 21.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18416159579018654 1101 2 0.16113081541764984 1101 3 0.11970828068665895 1101 4 0.0564567342096467 1101 5 -0.022268634136779042 1101 6 0.024916031422620222 1101 7 0.10039291929313521 1101 8 0.13425128031395417 1101 9 0.10338664089573513 1101 10-11 0.19321036462282848 1101 12-13 0.21341346176606635 1101 14-15 0.38214378545333005 1101 16-17 0.38843475838640273 1101 18-19 0.33888071712977563 1101 20-21 0.2687651061698446 1101 22-23 0.35447984240643393 1101 24-25 0.36992733864413907 1101 26-27 0.3609354184544671 1101 28-29 0.4459166109126045 1101 30-31 0.44779117855937045 1101 32-33 0.45091952596410323 1101 34-35 0.4162039396939363 1101 36-37 0.44566438203501946 1101 38-39 0.5357817186769367 1101 40-41 0.6780925010398988 1101 42-43 0.5579006372482809 1101 44-45 0.5357016461038242 1101 46-47 0.6864510445273382 1101 48-49 0.7313943823609961 1101 50-51 0.6271253020778786 1101 52-53 0.8236451088548478 1101 54-55 0.8219821202483786 1101 56-57 0.9024069907260852 1101 58-59 1.0418763334855754 1101 60-61 1.0248084864698157 1101 62-63 1.1671078867675604 1101 64-65 1.2372240555357408 1101 66-67 1.4222871184284394 1101 68-69 1.5109427926148413 1101 70-71 1.6619474913944607 1101 72-73 1.8810902691929279 1101 74-75 1.9271701731228745 1101 76-77 1.7974640967616295 1101 78-79 1.9298702764862945 1101 80-81 2.092141718898258 1101 82-83 2.2982601960528584 1101 84-85 2.387786034356335 1101 86-87 2.643884238890326 1101 88-89 2.8661096116575955 1101 90-91 3.0917237481184294 1101 92-93 3.3215946636617275 1101 94-95 3.415496799807805 1101 96-97 3.5004659694482037 1101 98-99 3.668900049959486 1101 100-101 3.5307507717718813 1102 1 0.17569300016563005 1102 2 0.13247089226231878 1102 3 0.12831409892550028 1102 4 0.08972995096910097 1102 5 0.04054011674536895 1102 6 0.15591788719511612 1102 7 0.12015951016064008 1102 8 0.1474050480548783 1102 9 0.212060417000707 1102 10-11 0.17954037847577808 1102 12-13 0.2733405377481901 1102 14-15 0.46338610752133746 1102 16-17 0.33498001571148706 1102 18-19 0.3091785555468789 1102 20-21 0.2310680937021914 1102 22-23 0.40944730104361327 1102 24-25 0.42194451107415176 1102 26-27 0.44733665737864214 1102 28-29 0.4318088192818763 1102 30-31 0.439642769034819 1102 32-33 0.3303955969539416 1102 34-35 0.32215090642071686 1102 36-37 0.290991625298048 1102 38-39 0.39270754005230657 1102 40-41 0.44030858091653613 1102 42-43 0.524999881870464 1102 44-45 0.540566514500803 1102 46-47 0.4476085499508997 1102 48-49 0.4970933028764293 1102 50-51 0.5102933148330422 1102 52-53 0.6255119898501675 1102 54-55 0.7403542679783257 1102 56-57 0.6968475145244142 1102 58-59 0.8054711739152722 1102 60-61 0.7664771134595512 1102 62-63 0.8957632358357515 1102 64-65 0.9415093217476098 1102 66-67 1.022567444626194 1102 68-69 1.149191309303717 1102 70-71 1.1959198900457615 1102 72-73 1.3626697668274694 1102 74-75 1.4947686466846548 1102 76-77 1.5515154310881911 1102 78-79 1.7365595522122668 1102 80-81 1.727649050506507 1102 82-83 1.8756831553565512 1102 84-85 2.1331760079573208 1102 86-87 2.1381764315770155 1102 88-89 2.37377774203215 1102 90-91 2.7103624997847042 1102 92-93 3.0216881057276446 1102 94-95 3.117929560726804 1102 96-97 3.3027706559272048 1102 98-99 3.7558501793408077 1102 100-101 3.5944285992111737 1103 1 0.1806187325124995 1103 2 0.16399075302985722 1103 3 0.20181890868004615 1103 4 0.11117230113735133 1103 5 0.059056182010088776 1103 6 0.02315851675512448 1103 7 0.07115187138495571 1103 8 0.10652718228281088 1103 9 0.2143282631772081 1103 10-11 0.16533765321906202 1103 12-13 0.28550983480452174 1103 14-15 0.3653952074695823 1103 16-17 0.37240514108324163 1103 18-19 0.19211755614819026 1103 20-21 0.2281519045308258 1103 22-23 0.34679170980552954 1103 24-25 0.3597572633135613 1103 26-27 0.30816748196357224 1103 28-29 0.18958339487945608 1103 30-31 0.21775507395941673 1103 32-33 0.3665784547946984 1103 34-35 0.35976391090568427 1103 36-37 0.385276406695894 1103 38-39 0.3680675513989229 1103 40-41 0.5884604955844495 1103 42-43 0.5847141677254513 1103 44-45 0.6148208708092326 1103 46-47 0.575883937885564 1103 48-49 0.7925820865180029 1103 50-51 0.8906135810057876 1103 52-53 0.8952869436683528 1103 54-55 1.0962408924136398 1103 56-57 1.1631802176542365 1103 58-59 1.2783572384068123 1103 60-61 1.4352915445453434 1103 62-63 1.5038213045172668 1103 64-65 1.418967275485521 1103 66-67 1.5882820427101727 1103 68-69 1.6369316431326482 1103 70-71 1.75229199607951 1103 72-73 1.8736312695845925 1103 74-75 1.8807009087541005 1103 76-77 1.9116533437143062 1103 78-79 1.9573694663197472 1103 80-81 2.1735149959275013 1103 82-83 2.246327277842898 1103 84-85 2.6280732309415136 1103 86-87 2.7513751978292973 1103 88-89 3.0699910052633435 1103 90-91 3.3397092665156727 1103 92-93 3.4478193571404354 1103 94-95 3.8445895822676484 1103 96-97 4.193638253600948 1103 98-99 4.3124150892351665 1103 100-101 4.171778897970977 1104 1 0.08001092277723387 1104 2 0.045746200033036644 1104 3 0.07053739169541018 1104 4 0.014318787880078787 1104 5 -0.036394229100693565 1104 6 -0.09861468492246672 1104 7 0.0023283646417553427 1104 8 0.09485571247527247 1104 9 0.0381367986997887 1104 10-11 0.09631867791078008 1104 12-13 0.1328182419492876 1104 14-15 0.2617392952883577 1104 16-17 0.13538743423129773 1104 18-19 0.14262152611010492 1104 20-21 0.22481454575221704 1104 22-23 0.2528625641424469 1104 24-25 0.3711364588652373 1104 26-27 0.36275869497834634 1104 28-29 0.3422222901524137 1104 30-31 0.22349191384094524 1104 32-33 0.4294517776999456 1104 34-35 0.35069180157700686 1104 36-37 0.4018311084113293 1104 38-39 0.4123938789717343 1104 40-41 0.3031223558601752 1104 42-43 0.4081531237283116 1104 44-45 0.39963830270514933 1104 46-47 0.4302433743975129 1104 48-49 0.3806364964234703 1104 50-51 0.650384339558471 1104 52-53 0.6414603331072044 1104 54-55 0.753068665107385 1104 56-57 0.6915210702593342 1104 58-59 0.6882694446990456 1104 60-61 0.8365183999761356 1104 62-63 0.749300339468995 1104 64-65 0.8628318143770386 1104 66-67 0.8922254468997046 1104 68-69 1.2575222832063062 1104 70-71 1.3330284064873545 1104 72-73 1.581019470619136 1104 74-75 1.5183681191112406 1104 76-77 1.4508283085090738 1104 78-79 1.586699779872685 1104 80-81 1.822111266981576 1104 82-83 1.882687495300516 1104 84-85 1.9390702181255506 1104 86-87 1.9911533758301232 1104 88-89 1.7657824881268773 1104 90-91 2.0023384162181266 1104 92-93 2.492852575189101 1104 94-95 2.63801970271993 1104 96-97 2.741840058876189 1104 98-99 3.1372518079819045 1104 100-101 2.89389201066162 1105 1 0.14484869514915744 1105 2 0.10716113058859378 1105 3 0.12109383090033532 1105 4 0.031644812913349085 1105 5 0.025708128683731957 1105 6 0.0195630221633607 1105 7 0.03234515397974036 1105 8 -0.008510471822795296 1105 9 0.09616397351682338 1105 10-11 0.053342131004590954 1105 12-13 0.14352664285581085 1105 14-15 0.2886386216356627 1105 16-17 0.2770370776527926 1105 18-19 0.28649133856851705 1105 20-21 0.2221655468749688 1105 22-23 0.20768663994917347 1105 24-25 0.22469496684927037 1105 26-27 0.24829686343310442 1105 28-29 0.31203851707357444 1105 30-31 0.427503162177608 1105 32-33 0.4232363439840441 1105 34-35 0.3624150997722779 1105 36-37 0.2868046509096729 1105 38-39 0.35385730628377843 1105 40-41 0.33383891752851014 1105 42-43 0.36892883792491915 1105 44-45 0.5659723013744795 1105 46-47 0.6133563151826067 1105 48-49 0.7759350919407666 1105 50-51 0.8962948622630549 1105 52-53 0.9678953536910342 1105 54-55 1.077666214799656 1105 56-57 1.1365983635893357 1105 58-59 1.1573527624385633 1105 60-61 1.1258284517476014 1105 62-63 1.2957192204570163 1105 64-65 1.3851524075656627 1105 66-67 1.3553967605224564 1105 68-69 1.6058724578570036 1105 70-71 1.8430539283602734 1105 72-73 1.935229670012017 1105 74-75 2.046154993279572 1105 76-77 1.9505729598314296 1105 78-79 2.109852692796835 1105 80-81 2.231155808107662 1105 82-83 2.278854146373373 1105 84-85 2.474986666478845 1105 86-87 2.5743924965470235 1105 88-89 2.956568234342779 1105 90-91 3.0303338576269745 1105 92-93 3.0689042478853743 1105 94-95 3.0900427399787453 1105 96-97 3.4426623725821095 1105 98-99 3.885747005087694 1105 100-101 4.1204220235525035 1106 1 0.16221220967141647 1106 2 0.11487395971454362 1106 3 0.11321728706211331 1106 4 0.08980213172442575 1106 5 -0.07057732869954236 1106 6 -0.01935951448159301 1106 7 0.07940634765509458 1106 8 0.0730482958210672 1106 9 0.15739751721931583 1106 10-11 0.18684511688950778 1106 12-13 0.15716607180532804 1106 14-15 0.0683261216356641 1106 16-17 0.029945982715226194 1106 18-19 -0.12686837573658494 1106 20-21 -0.14271489048930874 1106 22-23 -0.24522226511326295 1106 24-25 -0.05401194140263499 1106 26-27 -0.13603763202021213 1106 28-29 -0.040416991544780956 1106 30-31 0.058010932264465964 1106 32-33 0.13989495215451342 1106 34-35 0.17341855220170288 1106 36-37 0.16481046999219018 1106 38-39 0.08389344227726525 1106 40-41 0.08683137647258832 1106 42-43 0.03608813318822257 1106 44-45 0.08714493981096183 1106 46-47 0.22646027826621662 1106 48-49 0.3813514617154752 1106 50-51 0.5442051669590384 1106 52-53 0.5295154931454249 1106 54-55 0.6680942057741461 1106 56-57 0.7515731701854307 1106 58-59 0.9720101066975246 1106 60-61 0.9372225291770206 1106 62-63 0.9726757385080447 1106 64-65 1.1096801372752125 1106 66-67 1.172361591548512 1106 68-69 1.24149440411108 1106 70-71 1.24780038262195 1106 72-73 1.4353702695641353 1106 74-75 1.8133692135564452 1106 76-77 1.7504226052575973 1106 78-79 1.9203393411410268 1106 80-81 1.6369835853199284 1106 82-83 2.0807604048559973 1106 84-85 2.3717000724234687 1106 86-87 2.1488943542424614 1106 88-89 2.2653284559090103 1106 90-91 2.270328386332185 1106 92-93 2.000158701264855 1106 94-95 2.5067263719005695 1106 96-97 3.1071015012466034 1106 98-99 3.2456623484644567 1106 100-101 3.3359787384004953 1107 1 0.2383793832971861 1107 2 0.2507612746708787 1107 3 0.21291224930426011 1107 4 0.042092932949749695 1107 5 0.05882895788073483 1107 6 0.007880465081960608 1107 7 0.07402173061221617 1107 8 0.16079310748450126 1107 9 0.2151826628505944 1107 10-11 0.2424070438658319 1107 12-13 0.2960171034365473 1107 14-15 0.4024756914569707 1107 16-17 0.3533965957009926 1107 18-19 0.25791487499583354 1107 20-21 0.3755164510085507 1107 22-23 0.31564214506443733 1107 24-25 0.4878467840784779 1107 26-27 0.4406620103418746 1107 28-29 0.4652614533878179 1107 30-31 0.4451971906907346 1107 32-33 0.46411926161585626 1107 34-35 0.6021619475414184 1107 36-37 0.6487988979590824 1107 38-39 0.5945169480772918 1107 40-41 0.6204607652976435 1107 42-43 0.6990002844697045 1107 44-45 0.7502873245379504 1107 46-47 0.7962580910484789 1107 48-49 0.9283859126582854 1107 50-51 0.9009659031024597 1107 52-53 0.9726424427462916 1107 54-55 1.2131223058955172 1107 56-57 1.2488804305152144 1107 58-59 1.2708375976056772 1107 60-61 1.419175719354591 1107 62-63 1.382884263612759 1107 64-65 1.4217186733526646 1107 66-67 1.6449569131531625 1107 68-69 1.832869272880167 1107 70-71 1.8891460410615828 1107 72-73 1.9791711046993115 1107 74-75 1.8779522819129255 1107 76-77 2.0463573034642337 1107 78-79 2.030385538732201 1107 80-81 2.1309843656992093 1107 82-83 2.2700207044612846 1107 84-85 2.4203056295000245 1107 86-87 2.6055035415916983 1107 88-89 2.980177435272079 1107 90-91 2.968977166073369 1107 92-93 3.1700886611464583 1107 94-95 3.4019119491117706 1107 96-97 3.493854670729032 1107 98-99 3.646336880721929 1107 100-101 3.249137398993714 1108 1 0.12648239700668995 1108 2 0.1145941891264215 1108 3 0.07730096067197678 1108 4 0.053957302262361395 1108 5 -0.015923922270594915 1108 6 0.045138708087016255 1108 7 0.14174278733011647 1108 8 0.1518827626693664 1108 9 0.17629402208532952 1108 10-11 0.07479322166580715 1108 12-13 0.14906182839602877 1108 14-15 0.25189803710942016 1108 16-17 0.28852084022095426 1108 18-19 0.215602353820735 1108 20-21 0.18446171358424834 1108 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58-59 0.6407469073561458 2316 60-61 0.8716338832952104 2316 62-63 0.9369695066291754 2316 64-65 1.076296094607784 2316 66-67 1.2517437728850958 2316 68-69 1.3926684743405247 2316 70-71 1.5327960872430602 2316 72-73 1.6023763904790478 2316 74-75 1.9539202748729778 2316 76-77 2.0874111867087173 2316 78-79 1.972462869078889 2316 80-81 2.0742540510105876 2316 82-83 2.2989012216938853 2316 84-85 2.5602931568634624 2316 86-87 2.573931043937133 2316 88-89 2.8491885697365547 2316 90-91 2.9892817456764256 2316 92-93 3.144417211484278 2316 94-95 3.5721512564622593 2316 96-97 4.00469877368101 2316 98-99 4.556628987688423 2316 100-101 4.707073523094632 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 2.0 4 9.0 5 39.0 6 73.0 7 174.0 8 415.0 9 779.0 10 1399.0 11 2329.0 12 3691.0 13 5549.0 14 7213.0 15 10158.0 16 13656.0 17 18840.0 18 19728.0 19 15618.0 20 14844.0 21 15299.0 22 16422.0 23 18161.0 24 20431.0 25 22645.0 26 26022.0 27 30098.0 28 35790.0 29 42709.0 30 51312.0 31 61101.0 32 73836.0 33 93428.0 34 121824.0 35 168582.0 36 227346.0 37 280874.0 38 153526.0 39 7156.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63319319732108 18.992551308185593 14.956710052539938 22.417545441953397 2 16.055616353434587 20.083120892189545 39.65657281301595 24.20468994135992 3 19.497951668613695 24.089920387997036 29.845677821539322 26.566450121849943 4 13.026772183443343 16.96417051011863 36.33137344691229 33.67768385952573 5 13.569518039330909 37.37702758837296 33.945008155945736 15.10844621635039 6 32.28110099216803 35.940744413797376 16.911572637236628 14.866581956797967 7 28.75870526721241 33.14639395907742 19.82261525802251 18.272285515687663 8 26.897201215119548 34.43907609929674 19.164697020199498 19.49902566538421 9 26.46888240528802 16.620063589324534 20.097869624807807 36.81318438057964 10-11 25.254208665832216 26.761624785036588 26.27616639986642 21.708000149264777 12-13 26.258014285145055 24.08902631930139 27.152464481624307 22.500494913929252 14-15 22.914522170727967 25.337450765535635 26.067362076298988 25.680664987437407 16-17 21.978384436490682 28.59660119044984 26.873099008717443 22.551915364342037 18-19 21.86311534562069 26.56169853195563 29.908138914908328 21.667047207515356 20-21 22.45163599886913 25.661682530265644 30.436858786034637 21.44982268483059 22-23 22.308355438504616 25.637444831943775 30.221342653556626 21.832857075994987 24-25 22.023657991648076 25.88926187761788 30.329032982358495 21.758047148375546 26-27 21.507107607803253 26.33157746261501 30.15666845036819 22.00464647921355 28-29 21.62416520827812 26.5385498421019 30.149492468137346 21.687792481482628 30-31 21.7177719322768 26.130822986522528 30.102562610565037 22.048842470635634 32-33 21.412822729736515 26.171269945980065 30.070179743884413 22.345727580399004 34-35 22.213932252214413 26.322085049399295 29.76004701140431 21.70393568698198 36-37 22.22759480428797 25.918885465756482 29.70852705725795 22.14499267269759 38-39 21.730158172932324 26.19447240742094 29.78934627990154 22.286023139745197 40-41 22.17681945611141 26.06011593308166 29.793277551185643 21.969787059621282 42-43 21.91871194020174 26.086025620144436 29.579644303463194 22.41561813619063 44-45 21.92769819335615 26.427182863274503 29.666757430541125 21.978361512828222 46-47 22.30053703695505 26.739361564193658 28.83042825708707 22.129673141764222 48-49 22.177931202852726 26.59623182337676 28.85708719909075 22.36874977467976 50-51 22.364354053519012 26.93982542345974 28.60365331864298 22.092167204378267 52-53 22.56620936408778 26.769404262774316 28.496796183375572 22.167590189762336 54-55 22.091724469872865 26.96142454254457 28.652543857596346 22.29430712998622 56-57 22.204224701818124 27.237992716380298 28.364310010486488 22.193472571315088 58-59 22.256073843055677 26.72791371483976 28.428868060690043 22.58714438141451 60-61 22.0522894749991 27.04712529323255 28.604032805361896 22.296552426406457 62-63 22.013139095164515 27.051710757752755 28.66740708742046 22.26774305966227 64-65 21.783265015182632 26.965282657520195 28.60308408856461 22.648368238732566 66-67 21.737758232806247 27.071665434388958 28.503721815995746 22.68685451680905 68-69 22.128393389162596 26.801714408054544 28.51333953799024 22.55655266479262 70-71 22.038438209758755 26.951779255105517 28.2302067633388 22.779575771796924 72-73 22.004305280910565 27.028619334297442 28.138808783734426 22.828266601057567 74-75 21.949922932572168 27.445807715344483 27.91728833970133 22.686981012382017 76-77 21.972059657842124 27.096711557837256 28.311764784011974 22.61946400030865 78-79 22.071485178197474 27.169320016722715 28.214869175116043 22.544325629963765 80-81 22.110505407376348 27.38610482321905 27.968427930004854 22.534961839399752 82-83 22.128725928165412 27.472551451599543 27.874752774898187 22.52396984533686 84-85 22.140316278034224 27.4080431925334 27.876381866321235 22.575258663111143 86-87 22.196282888567932 27.43644292252028 27.687859790979292 22.679414397932497 88-89 22.081857815181113 27.541975974695827 27.505956360292284 22.87020984983078 90-91 22.078509801391604 27.449905158024627 27.701288573233878 22.770296467349883 92-93 22.070473213613706 27.143767910982536 27.763975705260258 23.021783170143504 94-95 21.846101691056067 27.1281457077206 27.82769785014449 23.19805475107885 96-97 22.09548403908465 26.908678303009143 27.734885182985565 23.26095247492064 98-99 21.85381792100731 27.059173996558055 27.43575131729327 23.651256765141362 100-101 22.164902158836696 26.49835587379031 27.728082586429682 23.608659380943315 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1733.0 1 1158.0 2 868.0 3 1119.5 4 836.0 5 694.5 6 1358.5 7 2878.5 8 4458.0 9 4996.5 10 3919.0 11 2618.5 12 2326.0 13 2374.5 14 2487.5 15 2593.5 16 2707.0 17 2866.0 18 3048.5 19 3219.0 20 3394.0 21 3847.0 22 4557.5 23 5403.0 24 6526.0 25 8018.5 26 10068.0 27 12481.0 28 14343.5 29 16247.0 30 18560.0 31 21546.0 32 25990.5 33 28151.5 34 28772.5 35 30491.5 36 33193.0 37 36418.0 38 38217.5 39 39473.0 40 41733.5 41 44969.5 42 47117.0 43 49013.0 44 51289.5 45 53819.5 46 55136.0 47 56444.5 48 57633.0 49 57061.5 50 56561.5 51 54764.5 52 53443.0 53 54416.0 54 56032.5 55 53029.5 56 47434.5 57 43426.0 58 40075.0 59 35714.5 60 29399.5 61 24488.5 62 20737.0 63 15835.5 64 11558.5 65 9010.0 66 6816.0 67 5241.0 68 4016.5 69 3019.5 70 2354.0 71 1621.0 72 1221.0 73 1046.5 74 820.0 75 631.0 76 416.0 77 234.0 78 162.0 79 107.5 80 61.5 81 37.0 82 15.0 83 8.5 84 6.5 85 3.5 86 3.0 87 4.5 88 2.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016001689980854897 3 0.002529911459423699 4 0.0024666636729381063 5 0.11808361736860115 6 0.0 7 0.0 8 2.529911459423699E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 3.1623893242796236E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.1623893242796236E-4 24-25 6.64101758098721E-4 26-27 0.006388026435044839 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01201707943226257 36-37 6.324778648559247E-4 38-39 0.007811101630970671 40-41 0.010815371489036312 42-43 0.006356402541802043 44-45 0.0036367477229215673 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 6.324778648559247E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 7.90597331069906E-4 70-71 0.0 72-73 9.487167972838871E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.2649557297118494E-4 82-83 0.0021504247405101443 84-85 0.00436409726750588 86-87 0.0027512787121232725 88-89 0.0 90-91 0.002023929167538959 92-93 0.0 94-95 0.0 96-97 1.2649557297118494E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1581083.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.577065308663975 #Duplication Level Percentage of deduplicated Percentage of total 1 79.29519038305526 45.65584355348117 2 12.355631533471602 14.228020074649649 3 3.64311330141665 6.292793174475868 4 1.4950455907289886 3.4432135046733237 5 0.8024420767291701 2.310112992912769 6 0.472687905953432 1.6329589459017821 7 0.3323655072667995 1.3395641362773423 8 0.23221351950930108 1.0696138382673395 9 0.17026878393977257 0.8823219203634375 >10 1.0752278955494627 11.605290107105805 >50 0.0816615841285716 3.2046970850443035 >100 0.038990099096868826 4.145143316345725 >500 0.0032947781835410053 1.3332373130013486 >1k 0.0015375631523191356 1.28726579305485 >5k 2.196518789027337E-4 0.914751042016577 >10k+ 1.0982593945136685E-4 0.6551732024287125 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10361 0.6553103157772235 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9026 0.5708745208189576 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5440 0.344067958481623 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2319 0.14667161686008895 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1885 0.11922207752534181 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1664 0.10524431671202587 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1656 0.10473833442014113 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.324778648559247E-5 0.0 14-15 0.0 0.0 0.0 1.2649557297118494E-4 0.0 16-17 0.0 0.0 0.0 1.2649557297118494E-4 0.0 18-19 0.0 0.0 0.0 1.2649557297118494E-4 6.324778648559247E-5 20-21 0.0 6.324778648559247E-5 0.0 2.529911459423699E-4 6.324778648559247E-5 22-23 0.0 6.324778648559247E-5 0.0 5.692300783703322E-4 6.324778648559247E-5 24-25 0.0 6.324778648559247E-5 0.0 0.0012333318364690531 6.324778648559247E-5 26-27 0.0 6.324778648559247E-5 0.0 0.0018974335945677742 6.324778648559247E-5 28-29 6.324778648559247E-5 6.324778648559247E-5 0.0 0.004838455666147824 6.324778648559247E-5 30-31 6.324778648559247E-5 6.324778648559247E-5 0.0 0.01227007057820494 6.324778648559247E-5 32-33 6.324778648559247E-5 6.324778648559247E-5 0.0 0.029062357890129738 6.324778648559247E-5 34-35 6.324778648559247E-5 6.324778648559247E-5 0.0 0.056322153865420096 6.324778648559247E-5 36-37 6.324778648559247E-5 6.324778648559247E-5 0.0 0.09816056462563952 6.324778648559247E-5 38-39 6.324778648559247E-5 6.324778648559247E-5 0.0 0.16030151484773414 6.324778648559247E-5 40-41 6.324778648559247E-5 6.324778648559247E-5 0.0 0.2283877569994744 6.324778648559247E-5 42-43 6.324778648559247E-5 6.324778648559247E-5 0.0 0.29359622486612025 9.487167972838871E-5 44-45 6.324778648559247E-5 6.324778648559247E-5 0.0 0.37578672340414765 1.2649557297118494E-4 46-47 6.324778648559247E-5 6.324778648559247E-5 0.0 0.4729036995527749 1.2649557297118494E-4 48-49 6.324778648559247E-5 6.324778648559247E-5 0.0 0.5723292199081262 1.2649557297118494E-4 50-51 6.324778648559247E-5 6.324778648559247E-5 0.0 0.679755585253905 1.2649557297118494E-4 52-53 6.324778648559247E-5 6.324778648559247E-5 0.0 0.7808571719511246 1.2649557297118494E-4 54-55 6.324778648559247E-5 6.324778648559247E-5 0.0 0.891920285019825 1.2649557297118494E-4 56-57 6.324778648559247E-5 6.324778648559247E-5 0.0 1.0162970571437426 1.2649557297118494E-4 58-59 6.324778648559247E-5 6.324778648559247E-5 0.0 1.149876382201314 1.2649557297118494E-4 60-61 6.324778648559247E-5 6.324778648559247E-5 0.0 1.2963266318087032 1.2649557297118494E-4 62-63 6.324778648559247E-5 6.324778648559247E-5 0.0 1.4386341514012864 1.2649557297118494E-4 64-65 6.324778648559247E-5 6.324778648559247E-5 0.0 1.583313462987079 1.2649557297118494E-4 66-67 6.324778648559247E-5 6.324778648559247E-5 0.0 1.7533867608468372 1.2649557297118494E-4 68-69 6.324778648559247E-5 6.324778648559247E-5 0.0 1.9364258549361417 1.2649557297118494E-4 70-71 6.324778648559247E-5 6.324778648559247E-5 0.0 2.129426475396927 1.2649557297118494E-4 72-73 6.324778648559247E-5 6.324778648559247E-5 0.0 2.321288635700972 1.2649557297118494E-4 74-75 6.324778648559247E-5 6.324778648559247E-5 0.0 2.514036265015815 1.2649557297118494E-4 76-77 6.324778648559247E-5 9.487167972838871E-5 0.0 2.7221847303399 1.2649557297118494E-4 78-79 6.324778648559247E-5 1.2649557297118494E-4 0.0 2.958573332329802 1.2649557297118494E-4 80-81 6.324778648559247E-5 1.8974335945677742E-4 0.0 3.202361925338518 1.2649557297118494E-4 82-83 6.324778648559247E-5 1.8974335945677742E-4 0.0 3.4480479519418017 1.8974335945677742E-4 84-85 6.324778648559247E-5 1.8974335945677742E-4 0.0 3.702525420866583 1.8974335945677742E-4 86-87 6.324778648559247E-5 1.8974335945677742E-4 0.0 3.9596909207170023 1.8974335945677742E-4 88-89 6.324778648559247E-5 1.8974335945677742E-4 0.0 4.239024769730621 1.8974335945677742E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3800 0.0 58.559986 1 GTATCAA 7015 0.0 43.583492 1 TGGTATC 1485 0.0 33.940495 2 ATCAACG 9115 0.0 33.175056 3 TATCAAC 9185 0.0 32.92431 2 TCAACGC 9265 0.0 32.635887 4 CAACGCA 9405 0.0 32.09953 5 AACGCAG 9755 0.0 31.208635 6 GTGGTAT 1670 0.0 30.465342 1 ACGCAGA 11160 0.0 27.151918 7 CGCAGAG 11235 0.0 26.886116 8 GTACCGT 135 1.3443323E-5 24.626818 6 GCAGAGT 12345 0.0 24.16087 9 TACATGG 6940 0.0 23.979897 2 TACCGTA 100 0.0016550188 23.747288 7 GTACATG 7085 0.0 23.690466 1 TAAGGTG 885 0.0 23.637043 5 GAGTACT 7840 0.0 22.778013 12-13 ACATGGG 7100 0.0 22.366568 3 TACCTGG 1115 0.0 21.748737 2 >>END_MODULE