FastQCFastQC Report
Thu 26 May 2016
SRR937966_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937966_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582691
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108540.6857940052732971No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89500.5654925693012723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53510.338095054562135No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22400.14153110114355866No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17930.11328806444214315No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17420.11006570455003536No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16530.10444237062067076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42600.056.6165051
GTATCAA75400.041.868881
ATCAACG96750.032.2301333
TATCAAC97300.032.1456532
TCAACGC97900.031.802984
CAACGCA99200.031.290375
AACGCAG102450.030.2939196
GTGGTAT17800.030.0510251
TGGTATC17700.029.0041222
ACGCAGA116650.026.5553677
CGCAGAG117100.026.4533188
GCAGAGT124950.023.992899
TACATGG73000.023.8974972
GTACATG74550.023.767721
GAGTACT78750.023.61576512-13
GGACCGA5850.023.5611446
GTACTTT83000.022.265214-15
ACATGGG75700.022.2288283
CAGAGTA122350.021.85805110-11
AGTACTT80600.020.80465112-13
Produced by FastQC (version 0.11.5)