FastQCFastQC Report
Thu 26 May 2016
SRR937966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937966_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582691
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105540.6668389470844277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91890.5805934323250717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55970.35363820227700793No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23930.1511981808198821No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18740.11840593015313791No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17090.10798064814925971No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16820.10627469291226146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42250.058.468891
GTATCAA72400.042.0598451
ATCAACG93450.032.5317733
TATCAAC94100.032.310122
TCAACGC94900.032.0316734
CAACGCA95800.031.7307515
AACGCAG99250.030.7713366
GTGGTAT16050.027.2308371
CGCAGAG116400.025.9927588
TGGTATC16450.025.991112
ACGCAGA116800.025.9850717
GTACATG74750.024.1501921
TACATGG73650.023.7368582
GAGTACT78500.023.68797912-13
GCAGAGT126550.023.645279
CAGAGTA120800.022.31335310-11
GTACTTT83050.022.19003114-15
ACATGGG74700.022.1291983
AGTACTT80900.020.4313312-13
AGAGTAC119900.019.82671510-11
Produced by FastQC (version 0.11.5)