##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937966_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1582691 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51651459444705 34.0 31.0 34.0 31.0 34.0 2 32.747456705067506 34.0 31.0 34.0 31.0 34.0 3 32.697021086238564 34.0 31.0 34.0 31.0 34.0 4 36.217937677032346 37.0 35.0 37.0 35.0 37.0 5 36.187042195855035 37.0 35.0 37.0 35.0 37.0 6 36.25291797324936 37.0 37.0 37.0 35.0 37.0 7 36.21513738310258 37.0 37.0 37.0 35.0 37.0 8 36.20996833873447 37.0 37.0 37.0 35.0 37.0 9 37.916475167926016 39.0 38.0 39.0 35.0 39.0 10-11 37.889744113032805 39.0 38.0 39.0 35.0 39.0 12-13 37.8313280987887 39.0 38.0 39.0 35.0 39.0 14-15 39.10792883765687 40.0 38.5 41.0 36.0 41.0 16-17 39.0411710182215 40.0 38.0 41.0 36.0 41.0 18-19 39.054117007046855 40.0 38.5 41.0 36.0 41.0 20-21 39.06803665402786 40.0 38.5 41.0 35.5 41.0 22-23 39.08722075250317 40.0 39.0 41.0 35.5 41.0 24-25 39.03604936149887 40.0 39.0 41.0 35.0 41.0 26-27 38.860413055991344 40.0 38.0 41.0 35.0 41.0 28-29 38.779705261481865 40.0 38.0 41.0 35.0 41.0 30-31 38.54147809016416 40.0 38.0 41.0 34.5 41.0 32-33 38.311080621548996 40.0 38.0 41.0 34.0 41.0 34-35 38.094535509458254 40.0 37.5 41.0 34.0 41.0 36-37 37.995671296544934 40.0 37.5 41.0 33.0 41.0 38-39 37.927017339455396 40.0 37.0 41.0 33.0 41.0 40-41 37.79192527157859 40.0 37.0 41.0 33.0 41.0 42-43 37.58729309764193 40.0 37.0 41.0 33.0 41.0 44-45 37.31045826380513 40.0 37.0 41.0 32.5 41.0 46-47 36.90639802715754 40.0 36.0 41.0 31.0 41.0 48-49 36.489198460091075 40.0 36.0 41.0 30.5 41.0 50-51 36.172452171649425 39.0 35.0 41.0 30.0 41.0 52-53 35.709050598000495 39.0 35.0 40.0 28.0 41.0 54-55 35.39458428714133 38.5 35.0 40.0 28.0 41.0 56-57 35.11874490977708 38.0 35.0 40.0 27.0 41.0 58-59 34.73826824061045 38.0 34.0 40.0 26.0 41.0 60-61 34.32980979862778 37.5 34.0 40.0 26.0 41.0 62-63 33.90086915260149 37.0 33.5 40.0 24.5 41.0 64-65 33.46276120859979 36.0 33.0 39.0 23.0 41.0 66-67 32.95521488401715 36.0 32.5 39.0 22.0 40.0 68-69 32.42386163818459 35.0 32.0 38.5 20.5 40.0 70-71 31.83536552618294 35.0 31.0 37.5 18.5 40.0 72-73 31.37219330873809 35.0 31.0 37.0 17.5 39.0 74-75 30.824255018825532 34.5 30.0 36.5 12.5 39.0 76-77 29.61096196288473 33.5 28.5 35.0 12.0 37.5 78-79 30.29236850402258 34.0 30.0 36.0 9.5 37.5 80-81 30.100259937031296 34.0 30.0 35.0 7.0 37.0 82-83 29.64852204252125 34.0 29.5 35.0 3.5 37.0 84-85 29.14963881136621 34.0 29.0 35.0 2.0 36.0 86-87 28.619809552212025 34.0 29.0 35.0 2.0 36.0 88-89 27.995630859087466 33.0 27.0 35.0 2.0 35.0 90-91 27.472816235133706 33.0 26.5 35.0 2.0 35.0 92-93 26.84399860743506 33.0 25.0 35.0 2.0 35.0 94-95 26.109535910673657 32.0 24.5 34.5 2.0 35.0 96-97 25.294757157272013 32.0 21.5 34.0 2.0 35.0 98-99 24.28547960404147 32.0 9.5 34.0 2.0 35.0 100-101 20.81424737993708 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.086734408287505 1101 2 0.07616756128879842 1101 3 0.2139533468145487 1101 4 0.1267009834293802 1101 5 0.003428012060794572 1101 6 0.017870124191382786 1101 7 0.03590214799298508 1101 8 0.09102009414331746 1101 9 0.03611068538621254 1101 10-11 0.11160091031197794 1101 12-13 0.06225808541421429 1101 14-15 0.14731679675424658 1101 16-17 0.20786100074660396 1101 18-19 0.23301141567707617 1101 20-21 0.20369446122283819 1101 22-23 0.1693416883610368 1101 24-25 0.20596731858607598 1101 26-27 0.10492281733667852 1101 28-29 0.18064013909531695 1101 30-31 0.222356880756152 1101 32-33 0.29175082860208335 1101 34-35 0.1407159169463128 1101 36-37 0.14136222375906016 1101 38-39 0.1616635222241598 1101 40-41 0.11242208975138368 1101 42-43 0.21158326658932225 1101 44-45 0.33831247058212455 1101 46-47 0.21980316775923114 1101 48-49 0.3289172014826818 1101 50-51 0.2642741408610121 1101 52-53 0.26150657938893573 1101 54-55 0.3133145293679789 1101 56-57 0.3970648060537343 1101 58-59 0.43051006526872015 1101 60-61 0.34394138767316207 1101 62-63 0.27648709382683734 1101 64-65 0.2763524132354078 1101 66-67 0.10075775515160501 1101 68-69 0.3041776233636071 1101 70-71 0.24679094562188197 1101 72-73 0.16634326139556066 1101 74-75 0.251559401791031 1101 76-77 0.4990038867274862 1101 78-79 0.6704188985863304 1101 80-81 0.7315535043431005 1101 82-83 0.6687086715525439 1101 84-85 0.6874542689372767 1101 86-87 0.7658383460507459 1101 88-89 0.8770684500573616 1101 90-91 1.1402504999540426 1101 92-93 1.3688543636858945 1101 94-95 1.3845582504940666 1101 96-97 1.5926290914434311 1101 98-99 1.8174987213673042 1101 100-101 2.053599065623729 1102 1 0.08245467030104692 1102 2 0.1586660475551085 1102 3 0.18592175106400788 1102 4 0.13244124507595245 1102 5 -0.04348442991275192 1102 6 -0.034826615317982146 1102 7 0.023845488482152177 1102 8 0.1334812416451996 1102 9 0.10250350386660756 1102 10-11 0.18479933786376534 1102 12-13 0.17380007546944398 1102 14-15 0.20470381717588992 1102 16-17 0.2126416469732746 1102 18-19 0.21939629852327158 1102 20-21 0.21989255850544964 1102 22-23 0.2649461268117008 1102 24-25 0.1695221157457567 1102 26-27 0.19951259550588674 1102 28-29 0.1314121432787232 1102 30-31 0.15034558738538806 1102 32-33 0.2660636852469125 1102 34-35 0.22789688610284742 1102 36-37 0.22494509379814076 1102 38-39 0.3596764027217674 1102 40-41 0.4116750851092732 1102 42-43 0.4458094734479161 1102 44-45 0.43373411422168573 1102 46-47 0.5098836249985439 1102 48-49 0.5386842241061203 1102 50-51 0.4541454276263934 1102 52-53 0.4698112435235373 1102 54-55 0.5705001773377418 1102 56-57 0.5791986201121944 1102 58-59 0.6480652477424371 1102 60-61 0.7949184339658899 1102 62-63 0.8477328049147133 1102 64-65 0.8804252742838301 1102 66-67 0.711427972211844 1102 68-69 0.8732453927623354 1102 70-71 0.9628200093087855 1102 72-73 0.9704333239632241 1102 74-75 0.8943306353463818 1102 76-77 0.9304867952979698 1102 78-79 0.9235204471817688 1102 80-81 1.0450190829563333 1102 82-83 1.078221341044845 1102 84-85 1.2536305776288579 1102 86-87 1.4330272508414872 1102 88-89 1.4277996293017807 1102 90-91 1.6097372983614129 1102 92-93 1.7702229165564844 1102 94-95 1.9690858279697068 1102 96-97 2.1250078551358413 1102 98-99 2.3745256566528923 1102 100-101 2.86107897424926 1103 1 0.2708418379565103 1103 2 0.26551069708268216 1103 3 0.37833001298447044 1103 4 0.21689521123723665 1103 5 0.10314817830522571 1103 6 0.1805710994699723 1103 7 0.16192503143974335 1103 8 0.2063828017418814 1103 9 0.22792099452799874 1103 10-11 0.3565390653961771 1103 12-13 0.22306397351383112 1103 14-15 0.3629767242292914 1103 16-17 0.3146192343167158 1103 18-19 0.4148396076860834 1103 20-21 0.39301110388009874 1103 22-23 0.5239084427862153 1103 24-25 0.5464612500019683 1103 26-27 0.5437418857717589 1103 28-29 0.5705189329952773 1103 30-31 0.6525126935159093 1103 32-33 0.5598837247986594 1103 34-35 0.608571463118885 1103 36-37 0.6190343049605218 1103 38-39 0.6516342141917733 1103 40-41 0.7671436634436972 1103 42-43 0.6180161313253052 1103 44-45 0.7568444196490702 1103 46-47 0.8360591908082498 1103 48-49 0.912121380774984 1103 50-51 0.9668514263080041 1103 52-53 0.9255380068722516 1103 54-55 0.9269328295021069 1103 56-57 0.8680746913053383 1103 58-59 1.0192968451935513 1103 60-61 1.1487747292856056 1103 62-63 1.2700053823702149 1103 64-65 1.1677851951803362 1103 66-67 1.0223678755297954 1103 68-69 1.188145964065349 1103 70-71 1.2488011123630542 1103 72-73 1.320007593354564 1103 74-75 1.2607061572097074 1103 76-77 1.0444727324546363 1103 78-79 1.4325047363489851 1103 80-81 1.4911051080057725 1103 82-83 1.5545232527126132 1103 84-85 1.522280324937146 1103 86-87 1.6928630012260157 1103 88-89 1.558606045474054 1103 90-91 1.4754151713563566 1103 92-93 2.034337946220294 1103 94-95 2.1149301478993934 1103 96-97 2.063686167594639 1103 98-99 2.1965153292523247 1103 100-101 2.7334394444755823 1104 1 0.1409110395559665 1104 2 0.11139327569068058 1104 3 0.16930577029194893 1104 4 0.07080310966286874 1104 5 0.07857497068567199 1104 6 0.06735492193615045 1104 7 0.07073975116588826 1104 8 0.013295584118345971 1104 9 0.129105832147701 1104 10-11 0.12020527465355713 1104 12-13 0.07706114052724189 1104 14-15 0.1457095234266106 1104 16-17 0.21058886824173584 1104 18-19 0.27880977279608743 1104 20-21 0.2538347357098729 1104 22-23 0.2122526211463267 1104 24-25 0.15281702590092294 1104 26-27 0.2013031564842862 1104 28-29 0.09997830472147484 1104 30-31 0.12229564542891325 1104 32-33 0.17913696724034622 1104 34-35 0.20864523881127184 1104 36-37 0.26697596314298266 1104 38-39 0.25531414572793665 1104 40-41 0.2583038599821421 1104 42-43 0.2153306961224999 1104 44-45 0.07012699530604039 1104 46-47 0.2513418254857811 1104 48-49 0.2118948893993604 1104 50-51 0.30374443679890817 1104 52-53 0.3347813633711496 1104 54-55 0.40014856438177304 1104 56-57 0.4165054823283185 1104 58-59 0.4679917531952569 1104 60-61 0.5048855698858432 1104 62-63 0.3765957649709577 1104 64-65 0.385116580835394 1104 66-67 0.2878674107429333 1104 68-69 0.5729230876986477 1104 70-71 0.65333684193088 1104 72-73 0.6336833934519497 1104 74-75 0.5083873871980522 1104 76-77 0.6839530564774776 1104 78-79 0.8276008660761605 1104 80-81 0.8570163115824201 1104 82-83 1.039089377865693 1104 84-85 1.1183045528745232 1104 86-87 0.9136323341462038 1104 88-89 1.1731782202339112 1104 90-91 1.110718315676369 1104 92-93 1.2035343813788408 1104 94-95 1.3989171338672541 1104 96-97 1.5320209026661296 1104 98-99 1.8360775442937118 1104 100-101 2.2971949223207417 1105 1 0.21743652523998236 1105 2 0.2060130573484713 1105 3 0.14502908034301498 1105 4 0.16255127540058112 1105 5 0.1264896367351369 1105 6 0.03997702032256001 1105 7 0.06250026833864553 1105 8 0.03663757087448971 1105 9 0.15560763497502705 1105 10-11 0.17398334866795295 1105 12-13 0.1536988276857869 1105 14-15 0.18461914022426384 1105 16-17 0.2615446578934595 1105 18-19 0.2596594847628424 1105 20-21 0.27834306143020626 1105 22-23 0.2050465046266723 1105 24-25 0.25209622879197724 1105 26-27 0.316350839329111 1105 28-29 0.31290128109834114 1105 30-31 0.27117698745498586 1105 32-33 0.32611348059714373 1105 34-35 0.3911668083856412 1105 36-37 0.4192389277915467 1105 38-39 0.47420902240460094 1105 40-41 0.5718965957063133 1105 42-43 0.49488502815651714 1105 44-45 0.5668223373313737 1105 46-47 0.49101400430488695 1105 48-49 0.5910631741782026 1105 50-51 0.7273118650632213 1105 52-53 0.7708752980747136 1105 54-55 0.7976179296700892 1105 56-57 0.7263256521104751 1105 58-59 0.8515579648617617 1105 60-61 0.769401009736022 1105 62-63 0.8291363958140039 1105 64-65 0.9507763273602095 1105 66-67 0.9441162168636055 1105 68-69 0.9141283281195243 1105 70-71 1.0128622649304404 1105 72-73 0.8689288995510687 1105 74-75 0.9301199040755641 1105 76-77 0.9686949912314873 1105 78-79 1.1974195225293407 1105 80-81 1.2603518666904066 1105 82-83 1.1355075358846918 1105 84-85 1.3516101789346386 1105 86-87 1.431333090402692 1105 88-89 1.5669150782884742 1105 90-91 1.5006597965800488 1105 92-93 1.6686285953806212 1105 94-95 1.7938101307519503 1105 96-97 2.1278791660543597 1105 98-99 2.46536405789902 1105 100-101 2.6182046391538947 1106 1 0.058526731339426874 1106 2 0.1209266854128046 1106 3 0.1445909223786188 1106 4 0.08307347559183853 1106 5 0.04403108621574603 1106 6 0.1296199347225624 1106 7 0.056021387590078575 1106 8 -0.005314458525269572 1106 9 0.016499051772136397 1106 10-11 0.04834202169885771 1106 12-13 0.006719921218440561 1106 14-15 -0.07573924070133131 1106 16-17 -0.007994390166125243 1106 18-19 -0.027950857266937135 1106 20-21 0.022538782500419074 1106 22-23 0.039924404231804544 1106 24-25 0.06737716614214406 1106 26-27 -0.03694058302475156 1106 28-29 -0.004137169046458666 1106 30-31 0.06744705045286992 1106 32-33 0.061349678527399476 1106 34-35 -0.1195890873169958 1106 36-37 0.12075367028644024 1106 38-39 0.1230798551637946 1106 40-41 0.031598828509736165 1106 42-43 0.07098015086139497 1106 44-45 0.11040985609951548 1106 46-47 -0.2942767662561536 1106 48-49 -0.1531201568210392 1106 50-51 -0.123835324098998 1106 52-53 -0.2239676164754698 1106 54-55 -0.23820664644868828 1106 56-57 -0.26405082958244463 1106 58-59 -0.2680231155749553 1106 60-61 -0.1980177968147956 1106 62-63 -0.20151269747684353 1106 64-65 -0.19277071717609573 1106 66-67 0.045837573178395985 1106 68-69 -0.4292448424207933 1106 70-71 -0.14650952843471643 1106 72-73 -0.09036859093391669 1106 74-75 -0.020006681149538963 1106 76-77 0.10850023433406619 1106 78-79 0.10462821600386718 1106 80-81 0.1854638372636117 1106 82-83 0.15196474151819217 1106 84-85 0.32003671125789523 1106 86-87 0.4517217031014624 1106 88-89 0.6531285712259454 1106 90-91 0.651270871125849 1106 92-93 0.522478134113296 1106 94-95 0.9181625148831962 1106 96-97 1.0170706313229267 1106 98-99 1.2319837424177784 1106 100-101 1.850007285597961 1107 1 0.06189585525824981 1107 2 0.06513746559236466 1107 3 0.17932529695865895 1107 4 0.11412346109980831 1107 5 0.036924181545686 1107 6 0.060225643346370816 1107 7 0.08922011577848821 1107 8 0.0965659455662049 1107 9 0.025406745186216995 1107 10-11 0.09821947508284268 1107 12-13 0.06268148356793546 1107 14-15 0.13847798280058754 1107 16-17 0.18291191724355116 1107 18-19 0.0924818841088566 1107 20-21 0.13716236389802816 1107 22-23 0.07947074929896303 1107 24-25 0.11255215646263395 1107 26-27 0.11784280293751692 1107 28-29 0.23036616847830516 1107 30-31 0.1629313493038893 1107 32-33 0.18828403727153642 1107 34-35 0.1074951676584206 1107 36-37 0.17785298389464543 1107 38-39 0.1589165681540976 1107 40-41 0.049552597853327995 1107 42-43 0.08266185073623689 1107 44-45 0.09642287059372023 1107 46-47 0.2687748115587638 1107 48-49 0.3010792258694437 1107 50-51 0.17962954163363776 1107 52-53 0.25743627959163007 1107 54-55 0.31197608384923825 1107 56-57 0.3840469738039616 1107 58-59 0.4558952307503574 1107 60-61 0.32241995684107394 1107 62-63 0.22170807021559824 1107 64-65 0.2630011775892669 1107 66-67 0.5034589266538276 1107 68-69 0.576921533315705 1107 70-71 0.7271334330528134 1107 72-73 0.5025872305388361 1107 74-75 0.675191300281778 1107 76-77 0.61671038543615 1107 78-79 0.851763770576337 1107 80-81 0.9903137993809459 1107 82-83 0.940253658059369 1107 84-85 1.2960731091283328 1107 86-87 1.308765845307839 1107 88-89 1.436507269326146 1107 90-91 1.4093589841040917 1107 92-93 1.6653653454622592 1107 94-95 1.8171521772371229 1107 96-97 1.694228944552659 1107 98-99 1.8622937628373712 1107 100-101 2.4322184856467786 1108 1 0.06502271092043088 1108 2 0.08713272893893986 1108 3 0.10815991688443916 1108 4 0.09798257403618749 1108 5 0.01670274092202817 1108 6 0.014630163514524952 1108 7 0.022785852416681962 1108 8 0.012093780671975196 1108 9 -0.014648566523504769 1108 10-11 -2.581379317518895E-4 1108 12-13 -0.0123962725705411 1108 14-15 0.11629516348136093 1108 16-17 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Count 2 3.0 3 7.0 4 11.0 5 28.0 6 67.0 7 147.0 8 365.0 9 672.0 10 1178.0 11 2006.0 12 3116.0 13 4799.0 14 6494.0 15 8775.0 16 11670.0 17 16730.0 18 16940.0 19 12251.0 20 10452.0 21 10693.0 22 11239.0 23 11836.0 24 13435.0 25 15352.0 26 18207.0 27 21391.0 28 26094.0 29 31067.0 30 38347.0 31 47052.0 32 59538.0 33 77575.0 34 108066.0 35 163372.0 36 244590.0 37 343110.0 38 229729.0 39 16287.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.57022311998994 18.956827327633757 15.010447396238433 22.46250215613787 2 16.07940079734446 20.001731434316397 39.69136235444735 24.22750541389179 3 19.699721217543832 24.191897363572256 29.672891881440368 26.43548953744354 4 13.001463962327453 16.944558350303378 36.38770928753623 33.666268399832944 5 13.571927722871118 37.409884209502074 33.90609381772506 15.11209424990175 6 32.193878002332745 35.973718096956695 16.941326327521693 14.891077573188872 7 28.73732143545392 33.239653223528784 19.79325086198127 18.22977447903602 8 26.882206875635784 34.471816969842486 19.169578376055956 19.476397778465778 9 26.6200414357572 16.607853333341758 20.05337744385986 36.71872778704118 10-11 25.205235892539985 26.790573775929733 26.28362074466842 21.72056958686187 12-13 26.295846757200238 24.040289608015716 27.199560748118234 22.464302886665813 14-15 22.927564508801783 25.321777908637884 26.06456345553238 25.686094127027953 16-17 21.93331545238946 28.608720403641776 26.854839907107547 22.60312423686122 18-19 21.83300467368551 26.555941747315178 29.931490101352697 21.679563477646617 20-21 22.45226642471588 25.60585104736174 30.4972669965268 21.444615531395577 22-23 22.25339627255099 25.563802409946096 30.33232639851999 21.850474918982922 24-25 22.00401728481336 25.843310727944896 30.37829958531865 21.774372401923088 26-27 21.499486671956365 26.32587625207646 30.26319240432491 21.911444671642265 28-29 21.628432650159933 26.51657386565573 30.192852347668126 21.66214113651621 30-31 21.677004544791117 26.148155262145295 30.18103344240916 21.993806750654425 32-33 21.38727483653771 26.163476192762925 30.13039071721213 22.318858253487235 34-35 22.19647393048656 26.30295431630228 29.86564510477192 21.634926648439233 36-37 22.23111431549435 25.903404906434503 29.778658491698074 22.086822286373074 38-39 21.71004422282998 26.210597995674096 29.831656215290455 22.24770156620548 40-41 22.144733029366435 26.041656462635547 29.87528593670162 21.938324571296395 42-43 21.84106933744027 26.05252397837917 29.71442077449991 22.39198590968065 44-45 21.856988762852136 26.38597072136259 29.765961735688478 21.991078780096796 46-47 22.271492437867874 26.717943355329066 28.8872720563635 22.12329215043956 48-49 22.10087089262466 26.61225075212305 28.94395068132571 22.342927673926578 50-51 22.31010348829936 27.013453668467186 28.630162173159512 22.046280670073944 52-53 22.548494936788043 26.744197066894298 28.58527027701554 22.122037719302128 54-55 22.066530990572385 26.909769500174068 28.76183664404486 22.261862865208688 56-57 22.16440227435425 27.15890214830311 28.55190937460313 22.12478620273951 58-59 22.221299040684507 26.686763240582025 28.529826731813095 22.562110986920377 60-61 22.019838363900472 27.01793969890522 28.682067440833364 22.280154496360947 62-63 21.95627573544046 27.0635582056131 28.79529232174821 22.184873737198227 64-65 21.711281608349324 26.943035627295536 28.722315347721068 22.623367416634075 66-67 21.65697114215111 27.137138123788056 28.576302735407904 22.629587998652926 68-69 22.040987180054213 26.79435012541938 28.689004163797076 22.47565853072933 70-71 21.8994421526375 27.002112225317514 28.318446241243556 22.77999938080143 72-73 21.928538166957416 27.07559466756303 28.2522930881644 22.743574077315152 74-75 21.942185808853402 27.446955849246628 27.95570961103589 22.65514873086408 76-77 21.986129952087932 27.07654242047247 28.28483260472196 22.652495022717638 78-79 22.078093576067594 27.20711117963014 28.2246502949723 22.49014494932997 80-81 22.043595370163853 27.420134441909383 28.041576024631464 22.494694163295296 82-83 22.0675438735053 27.44312635253606 27.99298655756867 22.49634321638997 84-85 22.072307168352566 27.438288122923954 27.888422929578926 22.600981779144554 86-87 22.125669948552375 27.440934745075985 27.77975930029965 22.653636006071988 88-89 22.026068899762777 27.591749618766272 27.625047348170728 22.757134133300223 90-91 22.019715810022316 27.52043502037894 27.77418848334601 22.685660686252735 92-93 21.997681155757462 27.19097609442182 27.853363113948763 22.957979635871954 94-95 21.811143173241017 27.182627562802846 27.92759294138906 23.07863632256707 96-97 22.065867118977764 27.009360350265197 27.821176208933355 23.103596321823684 98-99 21.814402856915397 27.161452113685215 27.553570322856206 23.47057470654318 100-101 22.039981246548493 26.743477046609403 27.803539903528403 23.413001803313698 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1811.0 1 1206.5 2 906.5 3 1160.5 4 855.5 5 708.5 6 1414.0 7 3038.0 8 4701.0 9 5243.0 10 4082.5 11 2702.5 12 2422.5 13 2485.5 14 2552.5 15 2625.0 16 2705.5 17 2801.5 18 2902.5 19 3091.0 20 3423.5 21 3904.0 22 4545.5 23 5323.5 24 6518.5 25 8012.0 26 9880.5 27 12164.0 28 14102.5 29 16266.5 30 18720.5 31 21548.0 32 25840.0 33 28147.5 34 28808.5 35 30685.0 36 33572.0 37 36685.0 38 38387.5 39 39880.5 40 42050.5 41 45115.0 42 47435.0 43 49148.5 44 51606.0 45 54451.0 46 55592.5 47 56744.0 48 57880.5 49 57132.0 50 56715.0 51 55239.5 52 53820.0 53 54418.0 54 55765.0 55 52845.5 56 47589.5 57 43401.5 58 39976.0 59 35555.5 60 28948.5 61 24126.0 62 20539.5 63 15531.0 64 11125.5 65 8628.0 66 6470.5 67 5074.0 68 3920.0 69 2828.5 70 2112.5 71 1511.5 72 1183.0 73 1013.0 74 784.0 75 552.0 76 367.5 77 226.5 78 144.0 79 79.5 80 46.5 81 33.0 82 19.0 83 9.0 84 4.0 85 3.0 86 3.5 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011878503131691531 3 0.006002435093141997 4 0.0 5 3.1591763648115774E-4 6 3.1591763648115774E-4 7 0.0 8 6.318352729623155E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.1591763648115774E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0018955058188869466 26-27 0.023346313335957554 28-29 2.2114234553681042E-4 30-31 0.0 32-33 0.003980562219662588 34-35 0.017533428824704253 36-37 0.008624551475935606 38-39 0.022967212172180167 40-41 0.024736350936474652 42-43 0.020724196953163947 44-45 0.010867566694951826 46-47 0.0022430152190162198 48-49 2.8432587283304194E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.263670545924631E-4 68-69 6.318352729623155E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0010109364367397048 84-85 0.0034119104739965037 86-87 8.529776184991259E-4 88-89 3.1591763648115774E-5 90-91 0.0033171351830521565 92-93 3.791011637773893E-4 94-95 0.0 96-97 2.8432587283304194E-4 98-99 3.1591763648115774E-4 100-101 0.003095992837515346 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1582691.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.64587125289993 #Duplication Level Percentage of deduplicated Percentage of total 1 79.2425630507504 45.680065873733625 2 12.444357428819323 14.34731652333575 3 3.575163545736199 6.182802523967104 4 1.5066715089107872 3.474135672923348 5 0.8050611126074714 2.3204224624043333 6 0.5027159123165579 1.7387698054910652 7 0.312840956159632 1.2623792636988578 8 0.23144176584134502 1.067332978898719 9 0.17694488184449605 0.9180127689900668 >10 1.079218368830544 11.581276898755183 >50 0.08063918361095261 3.191701450759065 >100 0.037673314514010625 4.139815324099033 >500 0.002956795152881907 1.2787315164655044 >1k 0.0014236421106468443 1.2175599702838757 >5k 2.1902186317643757E-4 0.9334184539128628 >10k+ 1.0951093158821878E-4 0.6662585122816417 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10554 0.6668389470844277 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9189 0.5805934323250717 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5597 0.35363820227700793 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2393 0.1511981808198821 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1874 0.11840593015313791 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1709 0.10798064814925971 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1682 0.10627469291226146 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.318352729623155E-5 0.0 10-11 0.0 0.0 0.0 6.318352729623155E-5 0.0 12-13 0.0 0.0 0.0 6.318352729623155E-5 0.0 14-15 0.0 0.0 0.0 6.318352729623155E-5 0.0 16-17 0.0 0.0 0.0 1.8955058188869464E-4 0.0 18-19 0.0 0.0 0.0 1.8955058188869464E-4 6.318352729623155E-5 20-21 0.0 0.0 0.0 1.8955058188869464E-4 6.318352729623155E-5 22-23 0.0 0.0 0.0 5.370599820179681E-4 6.318352729623155E-5 24-25 0.0 0.0 0.0 0.0010741199640359363 6.318352729623155E-5 26-27 0.0 0.0 0.0 0.0019902811098312934 6.318352729623155E-5 28-29 0.0 0.0 0.0 0.0047387645472173655 6.318352729623155E-5 30-31 0.0 0.0 0.0 0.013363316023152973 6.318352729623155E-5 32-33 0.0 6.318352729623155E-5 0.0 0.029538299010988246 9.477529094434732E-5 34-35 3.1591763648115774E-5 6.318352729623155E-5 0.0 0.05569627931162811 1.263670545924631E-4 36-37 6.318352729623155E-5 6.318352729623155E-5 0.0 0.10182025423787713 1.263670545924631E-4 38-39 6.318352729623155E-5 6.318352729623155E-5 0.0 0.17037438135428837 1.263670545924631E-4 40-41 6.318352729623155E-5 6.318352729623155E-5 0.0 0.23956034374366192 1.263670545924631E-4 42-43 6.318352729623155E-5 6.318352729623155E-5 0.0 0.30833561320561 1.263670545924631E-4 44-45 6.318352729623155E-5 6.318352729623155E-5 0.0 0.3870938799803626 1.263670545924631E-4 46-47 6.318352729623155E-5 6.318352729623155E-5 0.0 0.4811425603608032 1.263670545924631E-4 48-49 6.318352729623155E-5 6.318352729623155E-5 0.0 0.5823309793257181 1.263670545924631E-4 50-51 6.318352729623155E-5 6.318352729623155E-5 0.0 0.6918596238937353 1.263670545924631E-4 52-53 6.318352729623155E-5 6.318352729623155E-5 0.0 0.7947855898592966 1.263670545924631E-4 54-55 6.318352729623155E-5 6.318352729623155E-5 0.0 0.907315451973885 1.263670545924631E-4 56-57 6.318352729623155E-5 6.318352729623155E-5 0.0 1.0338088736209405 1.263670545924631E-4 58-59 6.318352729623155E-5 6.318352729623155E-5 0.0 1.1735708360002048 1.263670545924631E-4 60-61 6.318352729623155E-5 6.318352729623155E-5 0.0 1.3223996345464781 1.263670545924631E-4 62-63 6.318352729623155E-5 6.318352729623155E-5 0.0 1.4649100803631283 1.263670545924631E-4 64-65 6.318352729623155E-5 6.318352729623155E-5 0.0 1.610484927253646 1.263670545924631E-4 66-67 6.318352729623155E-5 6.318352729623155E-5 0.0 1.7772262557884009 1.263670545924631E-4 68-69 6.318352729623155E-5 6.318352729623155E-5 0.0 1.9583418367830485 1.263670545924631E-4 70-71 6.318352729623155E-5 6.318352729623155E-5 0.0 2.151146370327499 1.263670545924631E-4 72-73 6.318352729623155E-5 6.318352729623155E-5 0.0 2.349732196619555 1.263670545924631E-4 74-75 6.318352729623155E-5 6.318352729623155E-5 0.0 2.547401861765815 1.263670545924631E-4 76-77 6.318352729623155E-5 6.318352729623155E-5 0.0 2.758782352335358 1.263670545924631E-4 78-79 6.318352729623155E-5 6.318352729623155E-5 0.0 2.991518875131027 1.263670545924631E-4 80-81 6.318352729623155E-5 6.318352729623155E-5 0.0 3.2430524972973247 1.263670545924631E-4 82-83 6.318352729623155E-5 6.318352729623155E-5 0.0 3.5014099404116155 1.263670545924631E-4 84-85 6.318352729623155E-5 6.318352729623155E-5 0.0 3.757808694179723 1.263670545924631E-4 86-87 6.318352729623155E-5 6.318352729623155E-5 0.0 4.021220819477712 1.263670545924631E-4 88-89 6.318352729623155E-5 6.318352729623155E-5 0.0 4.312054595622266 1.263670545924631E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4225 0.0 58.46889 1 GTATCAA 7240 0.0 42.059845 1 ATCAACG 9345 0.0 32.531773 3 TATCAAC 9410 0.0 32.31012 2 TCAACGC 9490 0.0 32.031673 4 CAACGCA 9580 0.0 31.730751 5 AACGCAG 9925 0.0 30.771336 6 GTGGTAT 1605 0.0 27.230837 1 CGCAGAG 11640 0.0 25.992758 8 TGGTATC 1645 0.0 25.99111 2 ACGCAGA 11680 0.0 25.985071 7 GTACATG 7475 0.0 24.150192 1 TACATGG 7365 0.0 23.736858 2 GAGTACT 7850 0.0 23.687979 12-13 GCAGAGT 12655 0.0 23.64527 9 CAGAGTA 12080 0.0 22.313353 10-11 GTACTTT 8305 0.0 22.190031 14-15 ACATGGG 7470 0.0 22.129198 3 AGTACTT 8090 0.0 20.43133 12-13 AGAGTAC 11990 0.0 19.826715 10-11 >>END_MODULE