FastQCFastQC Report
Thu 26 May 2016
SRR937965_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937965_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584342
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108720.6862154762040014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91980.5805564707619946No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55750.3518810963794433No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22710.14334026365519567No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17840.11260195084142187No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17560.11083465564884351No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16330.1030711803385885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40600.058.4296231
GTATCAA73750.043.5567781
TATCAAC94950.033.8767932
ATCAACG95200.033.7878273
TCAACGC96600.033.298154
CAACGCA97300.033.009765
AACGCAG99500.032.27996
GTGGTAT16800.028.6955681
ACGCAGA113850.028.1891067
CGCAGAG114150.028.1566728
TGGTATC17150.027.7041242
GTACATG74250.026.3566471
TACATGG72450.026.2975042
GAGTACT77450.025.5357212-13
GCAGAGT123850.025.4907519
ACATGGG75350.023.961223
GTACTTT83200.023.52377314-15
TAAGGTG9000.023.228375
CAGAGTA119800.022.40359310-11
CATGGGG36100.022.1111154
Produced by FastQC (version 0.11.5)